![Qiagen Logo](/sfc/images/qiagen-logo.png)
Handling DNA
Working with DNA: Good laboratory practice
Handling DNA
TE Buffer, pH 7.4
Conversions for nucleic acids: DNA
Molecular weight conversions for DNA
- MW of a double-stranded DNA molecule (sodium salt) = (number of base pairs) x (662 daltons/base pair)
- MW of a single-stranded DNA molecule (sodium salt) = (number of base pairs) x (331 daltons/base pair)
- MW of a DNA oligonucleotide (sodium salt, pH ≥7):
- MW = (NA x 335.2) + (NC x 311.2) + (NC x 351.2) + (NT x 326.2) + P
Where NX = the number of residues of the respective nucleotide within the oligonucleotide (the MW listed for each nucleotide is the MW of that nucleotide, with associated sodium, incorporated in the oligonucleotide)
For dephosphorylated oligonucleotides: P = –84.0
For phosphorylated oligonucleotides: P = 40.0
Molecular conversions for DNA
Molar conversions for DNA can be found in the tables Microgram DNA conversions and Picomole DNA conversions. Protein/DNA conversions can be found in the table Protein/DNA conversions.