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Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
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Why do I observe a CT shift for the internal control in some samples in comparison to the CT value for the internal control of the dilution series of the Male Control DNA M1?
FAQ ID - 3734
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Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
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Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
FAQ ID -2575
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Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
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Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
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Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
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Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
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Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
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Why do I get smeared PCR products?
FAQ ID -87
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Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
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Why do I get different results for the loci SE33 if I analyze the same sample using the Investigator Nonaplex ESS Kit or Investigator Triplex AFS QS Kit?
FAQ ID -2401
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Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
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Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
FAQ ID -1844
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Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
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Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
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Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
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Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
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Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
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Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
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Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
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Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
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Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
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Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
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Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
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Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
FAQ ID -1847
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Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
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Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
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Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
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Why are the fluorescence intensities of the HSV detection markedly lower than those of the HSV-1 detection using the artus® HSV 1/2 LC PCR Kit?
-2
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Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
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Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
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Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
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Why are some of my RNAprotect-stabilized samples frozen atºC while others are not?
ID -758
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Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
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Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
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Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1837
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Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
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Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
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Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
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Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
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Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
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Why and when is it necessary to use carrier DNA/RNA in the extraction procedure?
FAQ ID -1489
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Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
FAQ ID -2568
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Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
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Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
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Which types of collection tubes should preferentially be used for blood samples?
FAQ ID -1496
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Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
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Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
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Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
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Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
FAQ ID -2392
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Which thermal cyclers are validated for use with the Investigator IDplex Kit?
FAQ ID -2403
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Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
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Which siRNA controls are available in FlexiTube format?
FAQ ID -1661
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Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator IDplex Plus Kit?
FAQ ID -3018
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Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
FAQ ID -3031
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Which settings for the filter set Any5Dye have to be adjusted before running the spectral calibration on the Investigator ESSplex Plus?
FAQ ID -2649
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Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
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Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
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Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
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Which sample materials can be tested using the artus Infl./H1 LC/RG RT-PCR Kit?
FAQ ID -2209
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Which sample materials are validated within the CE mark for the artus HSV/2 RG PCR
-1
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Which sample material is validated within the CE mark for artus VZV RG PCR Kit?
FAQ ID -2231
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Which Rotor-Gene Q System can be used to run the Investigator Quantiplex Pro RGQ Kit?
FAQ ID - 3742
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Which Rotor-Gene Q rotor should be used with the therascreen EGFR RGQ PCR kit?
FAQ ID -2312
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Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
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Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
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Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
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Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
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Which real-time PCR files should customers forward when they want their data to be re-analyzed by artus® technical support?
FAQ ID -1508
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Which real-time PCR cyclers can be used with the artus Infl./H1 LC/RG RT-PCR Kits?
FAQ ID -2208
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Which real-time cycler software version can be used to run the Investigator Quantiplex Pro RGQ Kit?
FAQ ID - 3743
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Which real-time cycler software version can be used to run the Investigator Quantiplex Pro Kit?
FAQ ID - 3730
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Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
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Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
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Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
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Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
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Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
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Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
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Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
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Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
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Which purity grade is recommended for pyrosequencing primers?
FAQ ID -2832
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Which purification kits are recommended for the therascreen Pyro kits?
FAQ ID -2386
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Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
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Which protocol line should I use to process plasma samples?
FAQ ID - 3654
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Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
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Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
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Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
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Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
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Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
FAQ ID - 3648
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Which pathogens can be detected using the artus Infl./H1 LC/RG RT-PCR Kit?
FAQ ID -2206
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Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
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Which operating system is compatible with PyroMark Q24 Advanced application software?
FAQ ID -3164
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Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
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Which network settings are supported by the QIAcube Connect and Q-Base?
FAQ ID - 141517
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Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
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Which Microbial qPCR Mastermix should I use?
FAQ ID — 3395
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Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
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Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?
FAQ ID - 3617
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Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
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Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?
FAQ ID -2930
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Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
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Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
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Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
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Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
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Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
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Which human target is used for the quantification?
FAQ ID - 3736
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Which human target is used for the quantification?
FAQ ID - 3747
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Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
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Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
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Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
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Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
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Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
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Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
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Which fixation method is used in the PAXgene Tissue System?
FAQ ID - 3600
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Which filter set should be used with the Investigator ESSplex Plus?
FAQ ID -2648
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Which filter set should be used with Investigator IDplex Plus Kit?
FAQ ID -3017
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Which filter set should be used with Investigator ESSplex SE Plus Kit?
FAQ ID -3030
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Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
FAQ ID -2396
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Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
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Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
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Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
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Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2938
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Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
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Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3655
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Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3512
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Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3384
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Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
FAQ ID - 3658
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Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
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Which DNA polymerases are included in artus® kits?
FAQ ID -1386
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Which disinfectants and cleaning agents do you recommend for the BioRobot EZ1 DSP?
FAQ ID -1561
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Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
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Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
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Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
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Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
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Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?
FAQ ID - 3605
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Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
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Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
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Which chemical products do you use to prepare screening solutions for protein crystallization?
FAQ ID -1122
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Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
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Which cell collection methods are compatible with REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3555
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Which cell collection methods are compatible with REPLI-g Single Cell DNA Library Kit?
FAQ ID - 3543
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Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
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Which BioRobot EZ1 instruments can be upgraded to become CE-IVDcompliant?
FAQ ID -1560
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Which barcodes on sample tubes are accepted by the QIAsymphony SP?
FAQ ID - 3598
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Which Anti-His Antibody is the most sensitive for my protein of interest?
FAQ ID -172
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Which analyses can be performed with the PyroMark Q96 MD software?
FAQ ID -2866
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Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
FAQ ID -2845
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Where to look for ordering Custom siRNAs online?
FAQ ID -3106
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Where to find explanations to the warning given by the PyroMark software after run data analysis?
FAQ ID -2874
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Where should I add a dye-label modification on the siRNA?
FAQ ID -648
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Where can I order the Streptavidin Sepharose beads for pyrosequencing?
FAQ ID -2850
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Where can I find the template files for the Investigator IDplex Plus Kit?
FAQ ID -3019
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Where can I find the template files for the Investigator ESSplex SE Plus Kit?
FAQ ID -3032
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Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
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Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
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Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?
FAQ ID - 141505
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Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
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Where can I find the gene list for a specific RT² Profiler PCR Array?
FAQ ID - 3684
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Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?
FAQ ID -1124
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Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?
FAQ ID - 3695
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Where can I find QIAGEN products for a specific gene or gene product?
FAQ ID -803
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Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
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Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
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Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
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Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
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Where can I find additional information for PreAnalytiX PAXgene products?
FAQ ID - 3515
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Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
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Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
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Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?
FAQ ID -806
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Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
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Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
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Where are the somatic mutations published?
FAQ ID -2429
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When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?
FAQ ID - 3348
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When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
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When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
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When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
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When should the N2 bottle used with the QIAxcel System be changed?
FAQ ID -1830
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When should the N2 bottle used with the QIAxcel Advanced System be changed?
FAQ ID -3001
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When should the EZ1 DNA Investigator Normalization protocol be used?
FAQ ID -1155
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