Product FAQ
Print
Bookmark
Share
more..
You are not authorized to download the resource
Sort options
Sort alphabetically
Sort by relevance
Sorted alphabetically
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
View
What is the maximum DNA input volume that can be used in the Investigator IDplex Plus Kit?
FAQ ID -3014
View
What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
FAQ ID -3027
View
What is the maximum DNA input volume that can be used in the Investigator ESSplex Plus Kit?
FAQ ID -2645
View
What is the maximum cDNA length that can be synthesized by QuantiTect Reverse Transcription Kit?
FAQ ID -3146
View
What is the maximum binding capacity of RNeasy spin columns?
FAQ ID -290
View
What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
View
What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
View
What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?
FAQ ID -307
View
What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
View
What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
View
What is the linear range of the Multi-Analyte ELISArray Kits?
FAQ ID -2909
View
What is the Limit of Detection (LOD) for an ELISArray Kit?
FAQ ID -2907
View
What is the length of the MDA whole genome amplification product?
FAQ ID -690
View
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
View
What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?
FAQ ID -1047
View
What is the label of the probe that is used in the Internal Control Assay to detect the Internal Control supplied with Certal Residual DNA Detection Kits?
FAQ ID -2976
View
What is the key technical challenge in isolating high quality RNA from cell or tissue samples?
FAQ ID -2656
View
What is the intended use of the QIAsymphony DSP AXpH kit?
FAQ ID -2273
View
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
View
What is the input sample volume when using the QIAsymphony DSP AXpH kit?
FAQ ID -2272
View
What is the Globally Harmonized System of Classification and Labelling of Chemicals (GHS) and what is the impact for me as a customer?
FAQ ID - 3428
View
What is the genotype of the EZ Competent Cells?
FAQ ID -157
View
What is the general sensitivity level of the LiquiChip System?
FAQ ID -731
View
What is the fixation reagent of PAXgene Tissue FIX?
FAQ ID - 3601
View
What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
View
What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?
FAQ ID -3140
View
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
View
What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
View
What is the expected yield from 8.5 ml of blood?
FAQ ID - 3496
View
What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3511
View
What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3383
View
What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
View
What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
View
What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?
FAQ ID - 3510
View
What is the expected DNA yield from 2.5 ml of blood collected in the PAXgene Blood DNA Tube (IVD)?
FAQ ID - 3382
View
What is the expected ccfDNA yield from plasma generated from 10 ml of blood?
FAQ ID - 3637
View
What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
View
What is the expected amplicon size of the Microbial DNA qPCR Assays?
FAQ ID — 3396
View
What is the error rate of HotStar HiFidelity DNA Polymerase?
FAQ ID -1048
View
What is the enzyme used in the whole transcriptome amplification step?
FAQ ID - 3554
View
What is the enzyme used in the whole genome amplification step?
FAQ ID - 3542
View
What is the enzyme used in the REPLI-g reaction?
FAQ ID -704
View
What is the Energy Mix in the EasyXpress Kits?
FAQ ID -846
View
What is the elution volume for all CE-marked artus QS-Rotor-Gene Q assays?
FAQ ID -2432
View
What is the effect of using amounts of input material per sample that fall outside the recommended range?
FAQ ID -
143064
View
What is the EasyXtal 15-Well Tool made of?
FAQ ID -1106
View
What is the dynamic range of detection of the LiquiChip Reader?
FAQ ID -1158
View
What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
View
What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3579
View
What is the dissociation curve temperature of PCR products generated with the miScript Plant qPCR system?
FAQ ID - 3444
View
What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
View
What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?
FAQ ID -1206
View
What is the difference between TurboCapture Microplate Holders Type A and Type B?
FAQ ID -1038
View
What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?
FAQ ID - 3715
View
What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
FAQ ID -295
View
What is the difference between the Microbial DNA qPCR Assays and the QuantiFast Pathogen +IC Kits?
FAQ ID — 3410
View
What is the difference between the Influenza Master and the Influenza H1 Master of the artus Infl./H1 LC/RG RT-PCR Kit?
FAQ ID -2207
View
What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?
FAQ ID -275
View
What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?
FAQ ID -3174
View
What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
View
What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version?
FAQ ID - 3685
View
What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
View
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
FAQ ID -761
View
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
View
What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?
FAQ ID — 3397
View
What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?
FAQ ID -764
View
What is the difference between LLOQ and LOD?
FAQ ID — 3402
View
What is the difference between HiPerFect and HiPerFect HTS?
FAQ ID -3108
View
What is the difference between EpiTect Bisulfite Kit and EpiTect Plus Bisulfite Kit?
FAQ ID -3167
View
What is the difference between disruption and homogenization in the RNeasy System?
FAQ ID -139
View
What is the difference between Buffers RLT and RLT Plus?
FAQ ID -1043
View
What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
View
What is the difference between adding the Internal Control (IC) template used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit to the amplification reaction versus adding the IC template at the extraction step?
FAQ ID -2600
View
What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
FAQ ID -2692
View
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
View
What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
View
What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
View
What is the detection limit for the Investigator Quantiplex Kit?
FAQ ID -2572
View
What is the delta Rn value?
FAQ ID -2681
View
What is the delivery time for QuantiTect Primer Assays?
FAQ ID -1142
View
What is the dead volume of the cartridges in PyroMark Q48?
FAQ ID - 3719
View
What is the data analysis method, and where can I find it for the qBiomarker Somatic Mutatation PCR arrays?
FAQ ID -2915
View
What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
FAQ ID -2395
View
What is the correct matrix to use with Investigator IDplex Plus Kit?
FAQ ID -3016
View
What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
FAQ ID -3029
View
What is the correct matrix to use with ESSplex Plus?
FAQ ID -2647
View
What is the correct matrix and filter/settings to be used on the Investigator ID plex kit?
FAQ ID -2405
View
What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?
FAQ ID -850
View
What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
View
What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
View
What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
View
What is the concentration of SureENTRY Transduction Reagent?
FAQ ID - 3708
View
What is the concentration of PyroMark Control Oligo?
FAQ ID -2846
View
What is the composition of the siRNA resuspension & annealing buffer?
FAQ ID -522
View
What is the composition of the QIAGEN Multiplex PCR Buffer?
FAQ ID -289
View
What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?
FAQ ID -606
View
What is the composition of the 5x miScript Plant RT Buffer?
FAQ ID - 3437
View
What is the composition of RBC Lysis Solution?
FAQ ID -2808
View
What is the composition of QIAzol? What is the color of this reagent?
FAQ ID - 3355
View
What is the composition of Protein Precipitation Solution?
FAQ ID -2810
View
What is the composition of PBS?
FAQ ID -361
View
What is the composition of gDNA Eliminator Solution?
FAQ ID -2799
View
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
View
What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?
FAQ ID - 3387
View
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
View
What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit?
FAQ ID -3122
View
What is the composition of DNA Hydration Solution in Gentra Puregene Kits?
FAQ ID -2813
View
What is the composition of Cell Suspension Solution?
FAQ ID -2811
View
What is the composition of Cell Lysis Solution?
FAQ ID -2809
View
What is the composition of buffer TE?
FAQ ID -416
View
What is the composition of buffer STE?
FAQ ID -415
View
What is the composition of Buffer S3?
FAQ ID -2788
View
What is the composition of Buffer RWT?
FAQ ID -2798
View
What is the composition of Buffer RW1?
FAQ ID -2796
View
What is the composition of Buffer RPE?
FAQ ID -2797
View
What is the composition of Buffer RLT?
FAQ ID -2793
View
What is the composition of Buffer RLT plus?
FAQ ID -2794
View
What is the composition of Buffer RLC?
FAQ ID -2795
View
What is the composition of Buffer RDD?
FAQ ID -2800
View
What is the composition of buffer QN?
FAQ ID -414
View
What is the composition of buffer QF?
FAQ ID -413
View
What is the composition of buffer QC?
FAQ ID -412
View
What is the composition of buffer QBT?
FAQ ID -411
View
What is the composition of Buffer PKD?
FAQ ID -2801
View
What is the composition of Buffer PE?
FAQ ID -1691
View
What is the composition of Buffer PB?
FAQ ID -2791
View
What is the composition of buffer P3?
FAQ ID -418
View
What is the composition of Buffer P2?
FAQ ID -203
View
What is the composition of buffer OW2?
FAQ ID -419
View
What is the composition of buffer OL2?
FAQ ID -424
View
What is the composition of buffer OEB?
FAQ ID -420
View
What is the composition of buffer OCL?
FAQ ID -422
View
What is the composition of buffer OCD?
FAQ ID -423
View
What is the composition of buffer OBB?
FAQ ID -421
View
What is the composition of Buffer N3?
FAQ ID -767
View
What is the composition of Buffer G2?
FAQ ID -2943
View
What is the composition of buffer FWB2?
FAQ ID -417
View
What is the composition of Buffer FRN?
FAQ ID -2802
View
What is the composition of Buffer FG3?
FAQ ID -356
View
What is the composition of Buffer ETR?
FAQ ID -2789
View
What is the composition of Buffer ER? Is it available separately?
FAQ ID -571
View
What is the composition of Buffer EB?
FAQ ID -199
View
What is the composition of Buffer BB?
FAQ ID -2790
View
What is the composition of Buffer B2?
FAQ ID -2945
View
What is the composition of Buffer B1?
FAQ ID -2944
View
What is the composition of Buffer APP?
FAQ ID -2804
View
What is the composition of Buffer APL?
FAQ ID -2803
View
What is the composition of Buffer ALO?
FAQ ID -2805
View
What is the composition of buffer AE?
FAQ ID -730
View
What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?
FAQ ID - 3429
View
What is the composition and size of the LiquiChip Beads?
FAQ ID -475
View
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
FAQ ID -2812
View
What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?
FAQ ID -2693
View
What is the cellular composition of human blood?
FAQ ID -2951
View
What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
FAQ ID -782
View
What is the blood draw volume of the PAXgene Blood ccfDNA Tube
FAQ ID - 3631
View
What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
View
What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
View
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
View
What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
View
What is the best way to ensure the Rotor-Disc is properly sealed when using the Heat Sealer?
FAQ ID -3179
View
What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?
FAQ ID -2705
View
What is the batch size of the QIASymphony Rotor-Gene Q and how many PCR´s can be processed per assay module run?
FAQ ID -2433
View
What is the basic technology behind the Strep-tag Protein Purification System?
FAQ ID -740
View
What is the bacterial ribosomal RNA recommended to use in the miRNeasy Serum/Plasma Protocol?
FAQ ID -3173
View
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
View
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
View
What is the average molecular weight of a siRNA, and how do I convert uM to ug values?
FAQ ID -388
View
What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
View
What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?
FAQ ID -721
View
What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
View
What is the amplicon size of the PCR product when using the miScript primer assay?
FAQ ID -3156
View
What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3578
View
What is the amplicon size of PCR products generated with the miScript Plant qPCR System?
FAQ ID - 3443
View
What is the amplicon length of the PyroMark CpG LINE assay?
-1
View
What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
View
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
View
What is the advantage of running an analytical gel with fractions of my plasmid preparation?
FAQ ID -769
View
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
View
What is the additive in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3372
View
What is the additive in PAXgene Blood DNA Tubes
FAQ ID - 3486
View
What is the adapted annealing protocol for PyroMark Q24 Advanced procedure?
FAQ ID -3166
View
What is the accuracy of the high-speed dispensing system of the BioRobot Universal?
FAQ ID - 3523
View
What is the acceptable number of reads per sample per miRNA sequencing run?
FAQ ID - 3673
View
What is the acceptable maximum g force for centrifugation of Xa Removal Resin?
FAQ ID -320
View
What is the "seed sequence" of a miRNA?
FAQ ID -2262
View
What is RNA interference (RNAi)?
FAQ ID -2755
View
What is REPLI-g whole genome amplification?
FAQ ID -654
View
What is qPCR?
FAQ ID -2668
View
What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
View
What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?
FAQ ID -3178
View
What is MOI?
FAQ ID -2768
View
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
View
What is LLOQ?
FAQ ID — 3401
View
What is included in the SureSilencing shRNA Plasmids shipment?
FAQ ID -2785
View
What is included in a PyroMark Custom Assay?
FAQ ID -2815
View
What is in the HiSpec Buffer that selectively synthesizes cDNA from mature miRNAs ONLY?
FAQ ID -3168
View
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
View
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
View
What is a quenched FRET assay?
FAQ ID -542
View
What is a QuantiTect Primer Assay?
FAQ ID -1141
View
What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?
FAQ ID -631
View
What is a PyroMark instrument method or instrument code?
FAQ ID -2941
View
What is a miScript Target Protector?
FAQ ID -2249
View
What is a miScript Precursor Assay?
FAQ ID -2214
View
What is a homogeneous LiquiChip assay?
FAQ ID -476
View
What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
View
What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
View
What instrument can I use for the therascreen EGFR RGQ PCR kit?
FAQ ID -2311
View
What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
View
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
View
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
View
What if only one heater shaker is available?
FAQ ID -
143764
View
What has to be done to a RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
View
What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
View
What happens if the starting volume is too low when using QIAsymphony mericon Bacteria Kit?
FAQ ID -2962
View
What happens if the starting volume is too high when using QIAsymphony mericon Bacteria Kit? Can spillage occur when the sample is initially transferred?
FAQ ID -2963
View
What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
View
What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
View
What format are the EasyXtal 15-well tools?
FAQ ID -2219
View
What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?
FAQ ID - 3364
View
What file format and layout do I need to upload my data into the PCR Array Data Analysis software?
FAQ ID -2700
View
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
View
What EZ1 DNA Investigator protocol is recommended for very precious samples?
FAQ ID -1154
View
What extraction procedure should I use for the therascreen EGFR RGQ PCR kit?
FAQ ID -2313
View
What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?
FAQ ID -170
View
What elution formats can be used for the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2986
View
What elution formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
FAQ ID -2937
View
What else do I need to complete an experiment using the SureSilencing shRNA Plasmids?
FAQ ID -2889
View
What effects can be predicted with poor sample quality? How robust is the platform with questionable sample quality (such as FFPE samples)?
FAQ ID -2918
View
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
View
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
View
What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
FAQ ID - 3506
View
What downstream applications can I use the eluates from the QIAsymphony DSP AXpH DNA Kit?
FAQ ID -2275
View
What does transcript variant mean when searching for specific QuantiTect Primer Assays?
FAQ ID -1137
View
What does the RT² qPCR Primer Assay product information mean when it says that it recognizes another transcript of the same gene?
FAQ ID -2712
View
What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?
FAQ ID - 3501
View
What does the Investigator Nonaplex ESS Kit contain?
FAQ ID -2391
View
What does the Investigator IDplex Plus Kit contain?
FAQ ID -3013
View
What does the Investigator IDplex Kit contain?
FAQ ID -2402
View
What does the Investigator ESSplex SE Plus Kit contain?
FAQ ID -3026
View
What does the ESSplex Plus kit contain?
FAQ ID -2644
View
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
View
What does PAXgene Tissue STABILIZER contain?
FAQ ID - 3513
View
What does PAXgene Tissue FIX contain?
FAQ ID -3020
View
What does a typical EpiTect Methyl qPCR Assay or Array include?
FAQ ID -2740
View
What does 'QIAGEN Certified Solution' for Protein Crystallization mean?
FAQ ID -1121
View
What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?
FAQ ID -942
View
What do you recommend for the cleanup of genomic DNA (gDNA)?
FAQ ID -618
View
What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
View
What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
View
What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box?
FAQ ID - 3697
View
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
View
What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
View
What do I need in order to set up an RNAi experiment?
FAQ ID -2757
View
What do I have to do if precipitates are visible in the lysis buffer (well 1 of the reagent cartridge [RCB])?
FAQ ID -1568
View
What do I have to consider if I am using the Applied Biosystems SDS software version 1.2.3 in order to run an Investigator Quantiplex Pro reaction?
FAQ ID - 3731
View
What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?
FAQ ID -2570
View
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
View
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
View
What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
View
What Disinfectant Solution do you recommend for the BioRobot MDx?
FAQ ID -809
View
What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
View
What dedicated QIAcube Kits are available?
FAQ ID -2337
View
What dedicated QIAcube Connect kits are available?
FAQ ID - 141504
View
What data documentation does QIAGEN's Sequencing Service provide for customers?
FAQ ID -832
View
What criteria should one use in choosing between siRNA versus shRNA for their studies?
FAQ ID -2771
View
What could have gone wrong if the CT values are unusually high for all wells in a sample?
FAQ ID — 3412
View
What could be a reason for higher viscosity of PCR reaction with STR Plus chemistry?
FAQ ID -3176
View
What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?
FAQ ID -3175
View
What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
View
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
View
What concentration should be used for the sequencing primer in pyrosequencing?
FAQ ID -2826
View
What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1205
View
What cell lines have been tested with the Fastlane Cell cDNA Kit?
FAQ ID -1175
View
What cell lines can I use with the SureSilencing shRNA Plasmids?
FAQ ID -2891
View
What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?
FAQ ID -2885
View
What can I use to isolate RNA smaller than 200 nucleotides?
FAQ ID -115
View
What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?
FAQ ID -572
View
What can be used to validate results obtained with the QIAseq miRNA Sequencing System?
FAQ ID - 3675
View
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
FAQ ID -728
View
What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
View
What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
View
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
View
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
View
What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
View
What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?
FAQ ID -1093
View
What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
View
What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?
FAQ ID -1221
View
What are your recommendations for cotransfecting several plasmids?
FAQ ID -124
View
What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
View
What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3613
View
What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
View
What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
View
What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
View
What are the temperature ramp rates of the LightCycler® (LC), the Rotor-Gene® (RG) and the ABI PRISM® PCR instruments (TM) for artus kits?
FAQ ID -1510
View
What are the technological principles behind somatic mutation PCR arrays and assays?
FAQ ID -2924
View
What are the SureSilencing shRNA Plasmids?
FAQ ID -2784
View
What are the structures of the siRNA molecules used in RNAi studies?
FAQ ID -2760
View
What are the storage conditions for the Microbial DNA qPCR products?
FAQ ID — 3394
View
What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
View
What are the stopping points in the QIAamp protocol?
FAQ ID -3157
View
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
View
What are the software requirements to run the QIAlink software package?
FAQ - 3426
View
What are the sequences of the FlexiTube siRNAs?
FAQ ID -851
View
What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?
FAQ ID -160
View
What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
View
What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?
FAQ ID -1077
View
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
FAQ ID -2993
View
What are the recommended culture and buffer volumes for a very low-copy plasmid?
FAQ ID -168
View
What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?
FAQ ID -1220
View
What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments?
FAQ ID -2657
View
What are the most commonly used protease inhibitors?
FAQ ID -53
View
What are the molecular weights of proteins in the 6xHis Protein Ladder?
FAQ ID -169
View
What are the miScript Controls that can be used with miScript HiSpec Buffer or miScript HiFlex Buffer?
FAQ ID -3111
View
What are the miScript Controls that can be used with miScript HiFlex Buffer ONLY?
FAQ ID -3112
View
What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
View
What are the minimal and maximum fragment sizes for the QIAquick column in the mericon DNeasy kit?
FAQ ID -3147
View
What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?
FAQ ID -167
View
What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
View
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
View
What are the main differences between other magnetic beads and the BioMag particles?
FAQ ID -272
View
What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
View
What are the key parameters contributing to unwanted off-target effects in an RNAi experiment?
FAQ ID -2775
View
What are the key factors for success in an RNAi experiment?
FAQ ID -2758
View
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
View
What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?
FAQ ID -2674
View
What are the features of the new PyroMark Q96 ID software 2.5?
FAQ ID -2867
View
What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
View
What are the features of PyroMark CpG Assays e.g. in terms of design, validation?
FAQ ID -2821
View
What are the features and benefits of the QIAexpress 6xHis Tag System?
FAQ ID -193
View
What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
View
What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID - 3475
View
What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
FAQ ID - 3481
View
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
View
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -2990
View
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
View
What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit? 8
FAQ ID -45
View
What are the expected average DNA yields from plant extractions on the BioSprint 96?
FAQ ID -771
View
What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
View
What are the dissociation constants for the Anti-His Antibodies?
FAQ ID -385
View
What are the dimensions of the TissueLyser LT?
FAQ ID -2545
View
What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
View
What are the dimensions and weight of the QIAcube?
FAQ ID -1407
View
What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
View
What are the differences between one-step and two-step RT--PCR?
FAQ ID -2666
View
What are the differences between new PyroMark Q24 Advanced reagents chemistry compared to PyroMark Q24 Gold reagents?
FAQ ID -3165
View
What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?
FAQ ID -665
View
What are the critical factors in designing the siRNA molecules to be used for RNAi studies?
FAQ ID -2759
View
What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
View
What are the control features on the qBiomarker Somatic Mutation PCR Arrays?
FAQ ID -2914
View
What are the compatibilities of different reagents with Ni-NTA matrices?
FAQ ID -49
View
What are the common primer and probe chemistries utilized for qPCR assays?
FAQ ID -2669
View
What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?
FAQ ID -2849
View
What are the calculated molecular weights of the TAGZyme enzymes?
FAQ ID -329
View
What are the benefits of PyroMark Q24 Advanced versus PyroMark Q24?
FAQ ID -3158
View
What are the applications the EasyXtal 15-Well Tool can be used for?
FAQ ID -1111
View
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
View
What are the advantages of using the SureSilencing shRNA Plasmids over siRNA?
FAQ ID -2786
View
What are the advantages of RT-PCR based somatic mutation PCR arrays and assays compared to other platforms?
FAQ ID -2921
View
What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
View
What are the advantages of EpiTect ChIP qPCR Assays and Arrays?
FAQ ID -2721
View
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
View
What are the additional plasmid bands I see on my gel?
FAQ ID -1059
View
What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
View
What are recommended stopping points during the QIAseq miRNA Library Kit procedure?
FAQ ID - 3663
View
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
View
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
View
What are possible reasons for blood draws with lower than expected blood volume?
FAQ ID - 3462
View
What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
View
What are Hot-Start DNA polymerases?
FAQ ID -1387
View
What are exo-resistant random hexamers used in the REPLI-g reaction?
FAQ ID -710
View
What are EpiTect ChIP qPCR Assays?
FAQ ID -2720
View
What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
View
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
View
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
View
What applications are offered on the QIAcube?
FAQ ID -1403
View
What annealing temperature should be used with the QuantiTect Primer Assays?
FAQ ID -849
View
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID -499
View
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID - 3474
View
What 96-well plates do you recommend for use with the LiquiChip Workstation?
FAQ ID -630
View
Were all assays on the qBiomarker Somatic Mutation PCR Arrays bench-validated?
FAQ ID -2912
View
We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyse the results. Any suggestions?
FAQ ID - 3621
View
Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit?
FAQ ID - 3660
View
There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?
FAQ ID - 3532
View
The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?
FAQ ID -776
View
The QIAsymphony RNA kit uses RLT plus as lysis buffer. Can samples lysed in RLT and then stored in the freezer be used on the QIAsymphony?
FAQ ID - 3533
View
The pathway reporter luciferase activity values are less than the negative control, is there a problem?
FAQ ID -2767
View
The pathway reporter luciferase activity values are greater than the positive control, is there a problem?
FAQ ID -2766
View
The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
View
The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why?
FAQ ID -3117
View
The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
View
The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions?
FAQ ID -2750
View
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
View
The concentrations of the quantitation standards (QS) of several artus® kits are stated as IU/µl (international units per µl). Is it possible to convert this concentration unit into copies per µl?
FAQ ID -1501
View
Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?
FAQ ID - 3659
View
Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?
FAQ ID -2031
View
Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3570
View
Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene?
FAQ ID -2882
View
Should I use the SureSilencing shRNA Plasmids with the GFP, neomycin, or hygromycin, or puromycin resistance marker?
FAQ ID -2900
View
Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?
FAQ ID -297
View
Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?
FAQ ID -147
View
Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays?
FAQ ID -2747
View
Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?
FAQ ID -1211
View
Should cells be washed before collection?
FAQ ID - 3544
View
Should cells be washed before collection?
FAQ ID - 3556
View
Should carrier DNA or RNA be used for nucleic acid extraction of Varicella Zoster Virus (VZV) and Herpes Simplex Virus (HSV) samples for artus® assays?
FAQ ID -1530
View
Should a negative control without the addition of IC be included in PCR run?
FAQ ID -1400
View
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
View
On which real-time cyclers is the Investigator Quantiplex PRO RGQ Kit validated?
FAQ ID - 3741
View
On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?
FAQ ID - 3728
View
On which instrumentation will the RT² Profiler PCR Array work?
FAQ ID -2719
View
On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
View
On which cyclers can Certal Residual Detection Kits be used?
FAQ ID -2973
View
On which basis are the species of Mycobacteria grouped in complexes?
FAQ ID -1535
View
On what cyclers have Certal Residual DNA Detection Kits been validated?
FAQ ID -2978
View
My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
View
My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?
FAQ ID -872
View
My BioMag is about to expire, is it still any good?
FAQ ID -268
View
My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
View
Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?
FAQ ID - 3559
View
May I try the data analysis tool without using your PCR array kit?
FAQ ID -2698
View
kemot solk - Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit? test
FAQ ID -1589
View
Is your Nuclease-Free Water fluorescence-free?
FAQ ID -1291
View
Is V600E the only mutation that can be detected with the therascreen BRAF Pyro Kit codon 600 assay?
FAQ ID -2388
View
Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
View
Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
View
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
View
Is there cross talk between channels in the Rotor-Gene Q?
FAQ ID -3185
View
Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?
FAQ ID - 3460
View
Is there also a miRNeasy Plus Universal Kit available?
View
Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
View
Is there a user manual available for the PyroMark Assay design software?
FAQ ID -2851
View
Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?
FAQ ID -837
View
Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
View
Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?
FAQ ID -2980
View
Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
View
Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?
FAQ ID -3088
View
Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?
FAQ ID -1071
View
Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
View
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
View
Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
View
Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?
FAQ ID -1040
View
Is the T790M mutation included in the therascreen EGFR PCR kit?
FAQ ID -2309
View
Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
View
Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide?
FAQ ID -1107
View
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
View
Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
View
Is the REPLI-g Single Cell RNA Library Kit compatible with adapters and amplification primers from other suppliers?
FAQ ID - 3552
View
Is the REPLI-g Single Cell DNA Library Kit compatible with adapters and amplification primers from other suppliers?
FAQ ID - 3540
View
Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?
FAQ ID -875
View
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
View
Is the QuantiFast SYBR Green PCR Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2652
View
Is the QuantiFast Probe PCR +ROX Vial Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2651
View
Is the QuantiFast Multiplex PCR +R Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2653
View
Is the quality and size of DNA extracted with the QIAamp DNA Mini and QIAamp Blood Mini kit good enough to generate DNA-libraries for next generation sequencing (NGS)?
FAQ ID - 3518
View
Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?
FAQ ID - 3517
View
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
View
is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
View
Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH?
FAQ ID -1123
View
Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
View
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
View
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
View
Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3573
View
Is the Microbial qPCR mastermix used in the Microbial DNA assay and in the Microbial DNA arrays free of genomic bacterial DNA?
FAQ ID - 3535
View
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
View
Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?
FAQ ID -3087
View
Is the Fast Reaction Mix ready to use in the Investigator ESSplex Plus?
FAQ ID -2639
View
Is the Fast Reaction Mix of the Investigator IDplex Plus Kit ready to use?
FAQ ID -3008
View
Is the Fast Reaction Mix of the Investigator ESSplex SE Plus Kit ready to use?
FAQ ID -3020
View
Is the expression level of miRNA precursors lower compared to that of mature miRNAs?
FAQ ID -2807
View
Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots?
FAQ ID -1118
View
Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?
FAQ ID -842
View
Is the CpG software included in the PyroMark instruments to study methylation status?
FAQ ID -2842
View
Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required?
FAQ ID - 3571
View
Is the annual preventive maintenance mandatory for the BioRobot EZ1 DSP?
FAQ ID -1556
View
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
View
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
View
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
View
Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?
FAQ ID -1217
View
Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?
FAQ ID -797
View
Is RNAprotect also suitable for the stabilization of miRNA?
FAQ ID -3143
View
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
View
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
View
Is RNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2960
View
Is REST software Windows 7 compatible?
FAQ ID -3182
View
Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?
FAQ ID -147909
View
Is QIAGEN carrier RNA sold separately?
FAQ ID -351
View
Is Q-Solution required for PCR with QIAGEN's PCR kits?
FAQ ID -380
View
Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?
FAQ ID -1
View
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
View
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
View
Is mRNA isolation necessary for sensitive RT-PCR?
FAQ ID -111
View
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
View
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
View
Is it possible to withdraw an aliquot of the LightCycler® sample for further gelelectrophoretic post-PCR analysis?
FAQ ID -1491
View
Is it possible to use the REPLI-g WTA Single Cell kit to amplify miRNA?
FAQ ID - 3563
View
Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?
FAQ ID -785
View
Is it possible to use the protein resuspended in ALO buffer for downstream BCA assay and Bradford assay?
FAQ ID -3138
View
Is it possible to use the PhosphoProtein Purification Kit in batch format?
FAQ ID -1069
View
Is it possible to use the native signal sequence of antibodies with EasyXpress Disulfide Insect Kits?
FAQ ID -2968
View
Is it possible to use templates with signal sequences with EasyXpress Disulfide E. coli Kits?
FAQ ID -2969
View
Is it possible to use RNAprotect Cell Reagent to stabilize buffy coat?
FAQ ID -3139
View
Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -876
View
Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?
FAQ ID -1225
View
Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system?
FAQ ID -3160
View
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
View
Is it possible to use Lyse&Spin baskets?
FAQ ID -
143762
View
Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
View
Is it possible to use LiquiChip beads for FACS applications?
FAQ ID -733
View
Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
View
Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?
FAQ ID -835
View
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3610
View
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
View
Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
View
Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
View
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
View
Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument?
FAQ ID -3162
View
Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?
FAQ ID -397
View
Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?
FAQ ID -147916
View
Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
View
Is it possible to store TAGzyme enzymes at°C?
-80
View
Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?
FAQ ID - 3680
View
Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?
FAQ ID - 3362
View
Is it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2996
View
Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?
FAQ ID -1063
View
Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system?
FAQ ID -3161
View
Is it possible to run the LightCycler® selftest after insertion of the carousel carrying the capillaries?
FAQ ID -1514
View
Is it possible to quantify protein content in RLT lysates?
FAQ ID -3155
View
Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
View
Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
View
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
View
Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?
FAQ ID - 3712
View
Is it possible to process samples on the AS-module while SP is running?
FAQ ID -2435
View
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
View
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID - 3606
View
Is it possible to perform laser microdissection with RNAprotect-stabilized tissue?
FAQ ID -608
View
Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?
FAQ ID -855
View
Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
View
Is it possible to partially use RNeasy 96 plates and use the plate multiple times?
FAQ ID -3124
View
Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
View
Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?
FAQ ID - 3709
View
Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
View
Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
View
Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy?
FAQ ID - 3599
View
Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
View
Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?
FAQ ID -576
View
Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID - 3476
View
Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
FAQ ID - 3483
View
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
FAQ ID -358
View
Is it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2995
View
Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?
FAQ ID -563
View
Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
View
Is it possible to introduce an affinity tag using the EasyXpress Linear Template Fab Kit?
FAQ ID -2972
View
Is it possible to interrupt the PAXgene Blood DNA kit protocol?
FAQ ID - 3503
View
Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?
FAQ ID - 3608
View
Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
View
Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?
FAQ ID -3090
View
Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?
FAQ ID -1232
View
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
View
Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?
FAQ ID - 3710
View
Is it possible to fix over a weekend?
FAQ ID -3027
View
Is it possible to extract RNA from RNAprotect-stabilized-blood?
FAQ ID -3120
View
Is it possible to extract RNA from frozen blood?
FAQ ID -3119
View
Is it possible to extend the number of PCR cycles to increase sensitivity of the Investigator IDplex Plus Kit?
FAQ ID -3010
View
Is it possible to extend the number of PCR cycles to increase sensitivity of the Investigator ESSplex SE Plus Kit?
FAQ ID -3022
View
Is it possible to extend the number of PCR cycles in the Investigator ESSplex Plus Kit to increase sensitivity?
FAQ ID -2641
View
Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
View
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
View
Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?
FAQ ID -854
View
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
View
Is it possible to edit the sample sheet after the run in the Rotor-Gene® software?
FAQ ID -1520
View
Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?
FAQ ID -1187
View
Is it possible to cleave the 6xHis-tag from an expressed protein?
FAQ ID -140
View
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
View
Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?
FAQ ID -763
View
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
View
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
View
Is it possible to analyze LightCycler® runs generated with an old software version with a newer version?
FAQ ID -1517
View
Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?
FAQ ID -841
View
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
View
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
View
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?
FAQ ID - 141496
View
Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
View
Is it necessary to include the external positive control (quantitation standards, QS) in the extraction procedure?
FAQ ID -1488
View
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
View
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
View
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
View
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID - 3607
View
Is it necessary to adjust the sample DNA concentration of artus® kits?
FAQ ID -1486
View
Is it mandatory to perform IQ/OQ?
FAQ ID -1559
View
Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
View
Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
View
Is it advisable to include the IC in the extraction procedure?
FAQ ID -1487
View
Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector?
FAQ ID -2783
View
Is GFP excited and detected by the green laser of the LiquiChip System?
FAQ ID -737
View
Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2959
View
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
View
Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?
FAQ ID -1049
View
Is double centrifugation required for plasma processing?
FAQ ID - 3643
View
Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
View
Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
View
Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
View
Is both fluorescence excitation and detection in the ABI PRISM® 7700 SDS mediated by glass fiber optics?
FAQ ID -1521
View
Is barcode reading supported by QS-RGQ?
FAQ ID -2437
View
Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?
FAQ ID - 3725
View
Is a weak or late (high) CT value of the IC an indicator for PCR inhibition?
FAQ ID -1398
View
Is a special processing protocol needed?
FAQ ID -2523
View
Is a special processing protocol needed?
FAQ ID -3035
View
Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?
FAQ ID -1213
View
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
View
Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
View
In which tubes is clot detection possible on QIAsymphony?
FAQ ID - 3589
View
In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
View
In which formats are crystallization condition Screening Suites available?
FAQ ID -1127
View
In which format can PyroMark CpG Assays be ordered?
FAQ ID -2824
View
In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
View
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
View
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
View
If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
View
If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?
FAQ ID -718
View
If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
View
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
View
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
View
If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
View
If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?
FAQ ID -266
View
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
View
If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?
FAQ ID -1145
View
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
View
If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?
FAQ ID -279
View
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
View
If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
View
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
View
If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?
FAQ ID -1136
View
If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?
FAQ ID -1857
View
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?
FAQ ID -1858
View
If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed?
FAQ ID -2977
View
If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)
FAQ ID -
143766
View
I would like to stop during the library preparation procedure. When can I do that?
FAQ ID - 3687
View
Show more results
Back to top
Contact QIAGEN
Global contacts
Technical Service
Customer Care
FAQListPage