QIAxcel Advanced System

DNAフラグメントおよびRNAの容易な解析

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QIAxcel Advanced Instrument

Cat. No. / ID: 9001941

Capillary electrophoresis device: includes computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor

InstrumentSoftware

QIAxcel Advanced

QIAxcel ScreenGel Software

The QIAxcel Advanced Instrument has been discontinued. QIAGEN will maintain a stock and inventory of repair and spare parts for the QIAxcel Advanced Instrument, including providing associated repair services until December 31, 2029.

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The QIAxcel Advanced instrument is intended to be used only in combination with QIAxcel Kits for the applications described in the respective QIAxcel Kit handbooks.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

マニュアル操作なしに最大96サンプルの高速解析
即使用可能なゲルカートリッジで安全かつ簡便なシステム
わずか0.1 ng/μl の核酸濃度でも正確な結果
3～5 bpの高い分離能で標準化された正確な解析
21 CFR Part 11 complianceに準拠したユーザーフレンドリーな解析ソフトウェア

Product Details

QIAxcel Advanced Systemは労力のかかる従来のDNA/RNAゲル解析に代わる革新的なシステムで、能率化したワークフローにより、結果を得るまでの時間を短縮します。QIAxcel Advanced Systemでは、1ランあたり最大96サンプルのキャピラリー電気泳動を高い分離能で完全自動化できます。12サンプルのDNAフラグメント解析をわずか3分で行なえます。即使用可能なゲルカートリッジを用いると、最小限のマニュアル作業で96サンプルを解析でき、マニュアル作業による間違いが減少し時間のかかるゲル調製も不要です。さらに、ユーザーフレンドリーなQIAxcel ScreenGel Softwareによりデータの解析とドキュメンテーションを簡便に行なえます。

Performance

能率的なワークフロー

QIAxcel Advanced Systemは、マニュアルによる電気泳動解析を排除することにより、DNAおよびRNA解析をスピードアップするだけでなく、同時にサンプル精製から解析までの全ワークフローを能率化します（図“能率的なワークフロー”）。QIAxcel Advanced を実証済みのエンドポイントPCR 用QIAGEN キットと組み合わせることによって、PCR フラグメントの解析を高い信頼性で実現するテスト済みの統合システムを提供します。高い再現性ならびに時間とコストの大幅な節約が可能です。

1台で多彩なアプリケーションに対応

QIAxcel Advanced System は多様性のある電気泳動装置で、様々なアプリケーションを提供します。プログラミング済みのメソッドと対応するゲルカートリッジを組み合わせることにより、複数のPCRフラグメント、制限酵素反応により切断されたDNA、トータルRNA、cRNAなどの様々な核酸の分離と解析を実現します。ジェノタイピングアプリケーションにおいては、QIAxcel Advanced Systemは従来のアガロースゲル電気泳動に比べて、より正確なサイズ測定と高感度な検出を実現します（図“バクテリアの正確なハイスループットジェノタイピング”）。QIAxcel Advanced System では多数のサンプルを処理できるので、品質管理のために迅速な結果を必要とし、96ウェルによるRNA精製を行っているラボに最適です（図“精製したRNAの解析”）。市販のその他の装置は最大12サンプルまでしか同時に処理できませんが、QIAxcel Advanced Systemは一回の操作で96サンプルの処理を手作業なしに行なうことができます。

Principle

QIAxcel Advancedには発光ダイオードアレイやmicro-optical collectorが搭載されています。キャピラリー内のゲルマトリックスを泳動するフラグメントは励起および検出スポットを通過し、シグナルが光電子増倍管からQIAxcel ScreenGel Softwareにデータ解析のために転送されます（図“サンプルの分離プロセス”）。

QIAxcel Advancedの高感度検出により、低濃度の核酸でも正確な結果が得られます。0.5 kb 未満のフラグメントの分離能が3～5 bp であるQIAxcel Advanced System は、より確実なデータ判断ができるだけではなく、スラブゲル法よりもさら高い精度を実現します。解析に必要なサンプル量は0.1 μl 未満で、残りの貴重なサンプルはダウンストリームアプリケーションに使用可能です。サンプルの自動アプライや充填済みゲルカートリッジにより、エチジウムブロマイドなどの有害物質への接触が最小限に抑えられます（図“すぐに泳動が可能なカートリッジ”）。

分離能やスピードなどの様々なアプリケーションのニーズに対応したQIAxcel Kitが入手可能です（下表参照）。

QIAxcel Kits
QIAxcel Kit	分析サンプル	サイズ範囲	100 bp ～ 500 kb*	500 bp ～ 1 kb*	1 bp ～ 5 kb*	5 bp ～ 10 kb*	泳動時間／12サンプル^†
QIAxcel DNA High Resolution Kit	DNA	15 bp ～ 10 kb	3～5 bp	50 bp	200～500 bp	1 ～1.5 kb	7～20分
QIAxcel DNA Screening Kit	DNA	15 bp ～ 5 kb	20～50 bp	100 bp	500 bp	–	5分
QIAxcel RNA QC Kit v2.0	RNA	15 bp ～ 6 kb	200 ～ 500 bpの間で最適な分離能	–	–	–	10分

Procedure

QIAxcel Advanced System は、ゲルカートリッジのセット、バッファートレイの充填とセット、96 ウェルプレート／PCRチューブ／ストリップチューブにサンプルをアプライ、使用するprocess profile を選択というような簡単なステップで成り立っています。手間のかかるゲル作製や専門的なトレーニングは不要なので、ラボのワークフローが能率化され、ルーチンワークへのインテグレーションが容易に行なえます。泳動開始数分後に、コンピューター画面に最初の結果がリアルタイムで表示されます（図“ラン時間の短縮”）。

Applications

QIAxcel Advanced System は広範なアプリケーションに至適化されているので、基礎研究および薬学、バイオテクノロジー、バイオメディカル研究に最適です。プログラミング済みのメソッドと対応するゲルカートリッジを組み合わせることにより、シングルあるいは複数のPCRフラグメント、制限酵素反応により切断されたDNA、トータルRNA、cRNAなどの様々な核酸の分離と解析を実現します。

Software

QIAxcel ScreenGel softwareはさらなる簡便性を提供

QIAxcel Advanced System用に開発されたQIAxcel ScreenGel Softwareは、データ収集や解析用にデザインされた高性能で使い易いツールです。この画期的なソフトウェアにより、解析とデータ解釈が容易に行なえ、データはゲルおよびエレクトロフェログラムの両フォーマットで表示可能です。結果は個々に表示することも、サンプルおよびデータ比較のために重ねて表示させることもできます。複数データセットの同時解析により評価を単純化できます。ユニークなアルゴリズムが計算を実施し、DNAフラグメントのピーク数、各ピークのサイズ、高さ、幅、面積などを含むピークの特性を示す表を作成します。包括的なデータレポートを容易に作成、保存でき、ニーズに合った個々のドキュメンテーションにエクスポートすることも可能です。サンプル泳動からデータ解析、レポート作製、データエクスポートまでをカバーする包括的なprocess profileでサンプルプロセッシングを標準化することにより、ユーザートレーニングを最小限に抑えることができます（図 "データ収集と解析の簡便化および標準化"）。

簡単なセットアップと操作の開始

Process Wizardにより実験を容易に開始できます（図 "使い易いソフトウェア"）。この便利な機能により、セットアップのガイドとランパラメーターの設定ができ、DNA size marker を前もって選択することができます。試薬のロット番号情報も含まれます。マウスを1回クリックするだけでサンプルを選ぶことができ、ランチェックも容易に行なえます。

21 CFR Part 11をサポート

QIAxcel ScreenGel Softwareは21 CFR Part 11の技術要件に準拠しており、電子記録システムの使用が可能です。セキュリティには以下のような特長があります：

不正アクセスやデータ操作を防ぐためにパスワードで保護されたログイン
構成ファイルとシステムイベントの監査証跡ドキュメンテーション
書き込み保護された生データの自動保存とアーカイブ
安全なユーザー管理

様々なユーザープロファイル（Routine、Basic、Advanced、Admin）があり、パスワードにより保護（ユーザーログインが必須）されているので安全です。簡略化されたインターフェイスによりユーザートレーニングの必要性を最小限に抑えられ、経験の浅いユーザーにとってこのソフトウェアは特に有効です。

Services

Are you thinking of upgrading to the latest QIAxcel generation, the QIAxcel Connect?

Then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply trade up your old QIAxcel Advanced to the QIAxcel Connect, or trade in a competitor instrument to the QIAxcel Connect.

Learn more about trade-in/trade-up opportunities.

Resources

For calibration of signal intensity

For optimal DNA size determination

For optimal RNA fragment size determination

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

For optimal use of the QIAxcel Gel Cartridges

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.

These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument

A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher

Addressing critical factors and new solutions

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Method file for use with QIAxcel ScreenGel Software

Alignment marker file QX15/600 bp - English (XAM)

Method file for use with BioCalculator Software

Method file for use with QIAxcel ScreenGel Software

Process Profile and Method file for use with QIAxcel ScreenGel Software

Method file for use with BioCalculator Software

The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.

For effortless DNA and RNA analysis

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

For easy upgrade of QIAxcel ScreenGel Software Version 1.6.

This study demonstrates that the QIAxcel Advanced capillary electrophoresis system in combination with the QIAxpert UV/Vis spectrophotometer can cover the comprehensive assessment of key quality parameters of nucleic acid samples.

FAQ

The QIAxcel System or QIAxcel Advanced works on the basis of fluorescence detection technology. Each channel has a separate excitation light source, with its own optical detection. So each gel cartridge channel is different in the fluorescence emission signal collection mode with its own background and/or baseline noise. When you use a new cartridge, calibration (intensity normalization) is necessary to equalize the variations.

FAQ ID -9019

First, check that the glass capillaries at the bottom of the cartridge are intact. You should see approximately 1 mm of glass capillary. Second, check that each tube has at least 10 µl sample volume.

Check the capillary is unblocked by performing the gel-droplet test as outlined in Appendix D in the QIAxcel DNA handbook.

Perform a detector test.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.

FAQ -9015

When the QIAxcel or QIAxcel Advanced is first installed and the Transport Locking assembly is removed, there is a possibility that the Transport lower stop is hitting the bottom. When the Transport Locking assembly is first removed, it is recommended to slowly pull up on the Transport assembly so that the lower stop is not hitting the bottom (be sure that the power is turned off before the Transport Lock is removed).

FAQ ID -9017

Yes, if a different method is used with the QIAxcel System or QIAxcel Advanced, a new reference marker table needs to be created because of different relative migration time.

Please follow the instructions described in the manual and run the DNA marker and the Calibration marker with the new method.

FAQ ID -1857

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
Analyze the samples immediately.

FAQ ID -1842

Make sure the Gel Cartridge has been calibrated with the computer currently connected. If not, please re-calibrate the Gel Cartridge.

Make sure the cartridge has equilibrated to room temperature (20°–25°C [68°– 77°F]) and stood upright in the cartridge stand for 20 min prior to use. Empty the buffer tray.

Wash the buffer tray in warm water and rinse thoroughly with deionized or reverse-osmosis water. Refill the buffer tray. Perform a control run with the alignment marker and bland samples (10 μl QX DNA Dilution Buffer). The marker bands should now appear within the chosen separation time.

FAQ ID -1833

Refer to Appendix D of the QIAxcel DNA Handbook for hot water soaking and gel droplet testing. The handbook can be accessed by going to the Resources tab in the link below:

http://www.qiagen.com/products/catalog/automated-solutions/detection-and-analysis/qiaxcel-dna-kits#

FAQ ID -1838

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824

Unplug the QIAxcel or QIAxcel Advanced instrument and remove the fuse holder to verify that the fuses are good. If not, please replace the fuses (Cat No. 9241178 Fuse, 4A, 250VAC, QX). If this does not correct the problem, contact QIAGEN Technical Services.

FAQ ID -1851

If you hear a 'hissing" sound when the QIAxcel System or QIAxcel Advanced is purging, remove the Gel Cartridge and verify that the Cartridge Purge Port label is removed.

FAQ ID -9018

If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.

FAQ ID -1834

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858

The revolutionary QIAxcel Advanced System replaces traditional, labor-intensive gel analysis of DNA and RNA.

Rapid analysis of up to 96 samples without manual intervention
Safety and convenience with ready-to-use gel cartridges
Robust results for nucleic acid concentrations as low as 0.1 ng/ul
Standardized and accurate analysis with a resolution down to 3-5 bp
User-friendly analysis software that supports 21 CFR Part 11 compliance

See: http://www.qiagen.com/Knowledge-and-Support/Videos-and-Virtual-Demos/Assay-Demos/QIAxcelAdvancedDemo/ for a virtual demonstration of the special software, features, and applications of the QIAxcel Advanced System.

FAQ ID -2650

The working alignment marker is an indication that the cartridge channel is working well. DNA samples that do not appear in all channels of the QIAxcel System or QIAxcel Advanced may be caused by:

Low sample volume (< 10 ul). The minimum required sample volume is 10 ul.
Trapped air bubble in the well. The air bubble must be removed.
High salt concentration in the sample. Dilute the sample with the QX DNA or RNA dilution buffer and rerun the sample.

FAQ ID -1844

Check that the lower and upper alignment markers are detected correctly, and that the threshold is set properly.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.

FAQ ID -9015

Verify that the Cartridge Purge Port label is removed. If it has been removed, verify that the alignment marker and samples are in the appropriate locations in the Buffer Tray and the 96-well Sample Tray used with the QIAxcel System or QIAxcel Advanced?.

FAQ ID -1847

When using this specific marker combination with the QIAxcel System or QIAxcel Advanced, i.e. for STR analysis, the 1.8 kb fragment lies outside of the range of detectable fragments and does not show up on the electropherogram and/or the gel view image.

FAQ ID -1835

N2 cylinders on the QIAxcel Advanced should be changed when one or both low pressure warnings are shown in the "Status Information" panel.

The left panel of the screen in the "Process" environment is called the "Status Information" panel.

"Pressure 1" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is sufficient for the current sample run; however, the N2 cylinder should be replaced once the run has finished.

"Pressure 2" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is insufficient for the current sample run and the analysis will not be performed. The N2 cylinder should be replaced.

FAQ ID -3001

The solutions used with the QIAxcel System or QIAxcel Advanced should not have to be changed for the life of the Gel Cartridge (based on the number of runs). However, if any contamination is suspected, the Buffer Tray should be cleaned and the buffers should be changed immediately.

FAQ ID -1840

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray.
Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day.
Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.

FAQ ID -1823

The minimum sample volume required for the QIAxcel System and QIAxcel Advanced is 10 ul to guarantee sample injection in each channel.

FAQ ID -1852

If one of the capillaries of the gel cartridge for the QIAxcel System or QIAxcel Advanced is clogged, the corresponding lane in the gel-view image is typically black.

FAQ ID -1836

When running a QIAxcel Advanced instrument with the BioCalculator software, ensure to use version v3.2.07 or higher. You can download the latest version of the software from:

BioCalculator: http://www.qiagen.com/resources/Download.aspx?id=b68d8727-f0d7-44e5-acc5-2bb6ed50b63d&lang=en&ver=1

ScreenGel: http://www.qiagen.com/Products/Catalog/Automated-Solutions/Detection-and-Analysis/QIAxcel-ScreenGel-Software#resources

FAQ ID -9016

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
Analyze the samples immediately.

FAQ ID -9020

Yes, the QIAxcel System or QIAxcel Advanced can be connected to a local network.

FAQ ID -1862

The BioCalculator (version 3.2.05) and ScreenGel software have a feature to close the pressure valve after the software is idled for more than 30 minutes. To re-open the pressure valve, execute a command with the software, such as move the buffer tray, unlatch and latch the cartridge; alternatively, you can also exit the software and re-launch it. Also see FAQ ID 3001.

FAQ -9011

The sample DNA concentration might be too low. You can increase sample injection time.

A very strong sample in the same run can cause the threshold to be set too high. As a result, the signal from weaker samples is below this threshold. Try diluting the sample with high concentration with the QX dilution buffer.

High salt concentration in the samples, such as restriction enzyme digested DNA, can interfere with sample injection. Try reducing the salt concentration by diluting such samples with the QX dilution buffer.

If the issue persists, please send data files to QIAGEN Technical Services. See <a href="/knowledge-and-support/faq/?ID=71818D37-21EF-40B0-BA30-6C8CC8CA82B9"><b>Where are the QIAxcel data stored</a></b>.

FAQ -9013

High or very low salt concentration could lead to the described appearance when running samples on the QIAxcel System and QIAxcel Advanced. Make sure QX DNA Dilution buffer has been used to dilute your samples. Dilute your samples 1:2 in QX DNA Dilution buffer and re-analyze.

FAQ ID -1837