DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples.

In this webinar, we present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries, especially for whole genome sequencing applications. The following technologies will be highlighted:
- Enzymatic DNA fragmentation technology
- Enhanced ultralow-input ligation chemistry
- Phi29-based MDA technology for whole genome amplification