illustration of people in lab coats working on dna

PCR

Cell line development (CLD)

Despite advancements in CRISPR and other cell line development technologies, the path from starting cell to a modified one remains long and tricky. What if there is a way to improve and accelerate your cell line development workflow while uncovering any unintended changes to the genome?

The Insight Exchange on Cell Line Development

Find out the best practices in cell line development and cell and gene therapy from renowned global scientists

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Overview

The vulnerabilities of cell line engineering

Given the low success rate of gene editing techniques, screening for successfully edited clones and characterizing the edits could take several months to complete. We are here to make your cell line development process easier and faster.
Gene editing, viral transduction or even routine transfections can cause off-target effects such as chromosomal rearrangements and structural variants, often missed by classical methods like karyotyping and FISH. Choosing your genome assessment method wisely when working with cell line development is indeed an important decision in getting the cell right.

Don’t sacrifice speed for quality – instead, combine both in your clone selection and cell line characterization workflow

Cell line developement benefits illustration

Clone selection and enrichment

Is CRISPR screening keeping you occupied for weeks?

Slash unnecessary tasks from your CRISPR gene edit characterization. Watch the video to learn how.

Analyze gene expression directly from cells and make your edits faster and easier

Take a 3X faster route to your clone of interest

Characterize CRISPR gene edits in 3 days using only 10 cells/µL.

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Gene expression analysis – validate gene edits at the transcript level using QuantiNova PCR

Perform qPCR directly from cells

Minimize the time and effort needed to identify your clone of interest. Streamlining your cell culture and gene editing process can help you obtain the desired clone early on.

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Cell line characterization

Reveal the true genome status within 5 days

Watch this video to discover a simple yet elegant approach to look for unintended genome changes in your engineered cells*.

Identify chromosomal alterations early on during the cell line development process
*Solely for the US: No rights to use this product to perform or offer any service in metagenomics or structural variation analysis are granted by the supply of this product, whether expressly, by implication or by estoppel.

Why choose EpiTect Hi-C over karyotyping or FISH?

Compared to classic methods, EpiTect Hi-C gives you a 10X sharper view into the genome, uncovering genomic blind spots such as chromosomal rearrangements and structural variants*. Learn more in this flyer.

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*Solely for the US: No rights to use this product to perform or offer any service in metagenomics or structural variation analysis are granted by the supply of this product, whether expressly, by implication or by estoppel.