FAQ 

 

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There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?
FAQ ID - 3532
Can I add bleach to the sample preparation waste?
FAQ ID - 3505
What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
FAQ ID - 3506
Can purified RNA be used with the miScript Single Cell qPCR Kit?
FAQ ID - 3566
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What is the recommended range of cells for the miScript Single Cell qPCR Kit?

FAQ ID - 3565

Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression?
FAQ ID - 3567
Can the miScript Single Cell qPCR Kit be used with plant cells?
FAQ ID - 3568
Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components?
FAQ ID - 3569
Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3570
Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required?
FAQ ID - 3571
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I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do?
FAQ ID - 3572
When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3576
Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3573
Does the miScript Single Cell qPCR Kit contain controls?
FAQ ID - 3574
I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause?
FAQ ID - 3577
What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3578
What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3579
Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA?
FAQ ID - 3580
When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3575
How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays?
FAQ ID - 3581
What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit?
FAQ ID - 3582
How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential?
FAQ ID - 3593
Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
How can I determine if a tube is suitable for clot detection on QIAsymphony?
FAQ ID - 3590
In which tubes is clot detection possible on QIAsymphony?

FAQ ID - 3589

Which barcodes on sample tubes are accepted by the QIAsymphony SP?
FAQ ID - 3598
How can I create a report/logfile on my QIAsymphony SP system?
FAQ ID - 3594
Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy?
FAQ ID - 3599
Can I change the language on my QIAsymphony SP system?
FAQ ID - 3595
Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit?
FAQ ID - 3587
Can the Rotor-Gene Q be connected to LIMS?
FAQ ID - 3596
Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans?
FAQ ID - 3584
What is MOI?
FAQ ID -2768
What is RNA interference (RNAi)?
FAQ ID -2755
What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?
FAQ ID -2705
What file format and layout do I need to upload my data into the PCR Array Data Analysis software?
FAQ ID -2700
What are the key factors for success in an RNAi experiment?
FAQ ID -2758
What does the RT² qPCR Primer Assay product information mean when it says that it recognizes another transcript of the same gene?
FAQ ID -2712
What do I need in order to set up an RNAi experiment?
FAQ ID -2757
What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
What are the SureSilencing shRNA Plasmids?
FAQ ID -2784
What criteria should one use in choosing between siRNA versus shRNA for their studies?
FAQ ID -2771
What does a typical EpiTect Methyl qPCR Assay or Array include?
FAQ ID -2740
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
What is the composition of Buffer RPE?
FAQ ID -2797
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products?
FAQ ID -2715
Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741
Will pipetting error affect the miRNA qPCR Assay results?
FAQ ID -2728
Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
What is the composition of Buffer S3?
FAQ ID -2788
What MOI should I use for my cells?
FAQ ID -2770
What RT² qPCR Primer Assays are available?
FAQ ID -2708
What transfection reagents are compatible with the Cignal Reporter Assays?
FAQ ID -2764
What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture?
FAQ ID -2777
What is included in the SureSilencing shRNA Plasmids shipment?
FAQ ID -2785
What is the composition of Buffer RLC?
FAQ ID -2795
What is the composition of Buffer RLT?
FAQ ID -2793
What is the composition of Buffer RLT plus?
FAQ ID -2794
What is the composition of Buffer BB?
FAQ ID -2790
What is the composition of Buffer ETR?
FAQ ID -2789
What is the composition of Buffer PB?
FAQ ID -2791
What are the key parameters contributing to unwanted off-target effects in an RNAi experiment?
FAQ ID -2775
What are the structures of the siRNA molecules used in RNAi studies?
FAQ ID -2760
What are the critical factors in designing the siRNA molecules to be used for RNAi studies?
FAQ ID -2759
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
What is the composition of gDNA Eliminator Solution?
FAQ ID -2799
What is the composition of Buffer RW1?
FAQ ID -2796
What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?
FAQ ID -2739
What is the composition of Buffer RWT?
FAQ ID -2798
Can miScript miRNA qPCR Assays be used for pre-miRNA detection?
FAQ ID -2724
Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs?
FAQ ID -2723
Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array?
FAQ ID -2736
Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays?
FAQ ID -2725
Do you always run samples in triplicates?
FAQ ID -2703
Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit?
FAQ ID -2722
Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids?
FAQ ID -2787
Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides?
FAQ ID -2737
Can I make stable cell lines with the Cignal Reporter Assays?
FAQ ID -2763
Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector?
FAQ ID -2783
How are the primers in the EpiTect Methyl qPCR Array designed?
FAQ ID -2735
On which instrumentation will the RT² Profiler PCR Array work?
FAQ ID -2719
Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays?
FAQ ID -2747
What are the advantages of using the SureSilencing shRNA Plasmids over siRNA?
FAQ ID -2786
The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions?
FAQ ID -2750
The pathway reporter luciferase activity values are greater than the positive control, is there a problem?
FAQ ID -2766
The pathway reporter luciferase activity values are less than the negative control, is there a problem?
FAQ ID -2767
How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays?
FAQ ID -2742
How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?
FAQ ID -2729
How can RNA interference be used as a life science research tool?
FAQ ID -2756
How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system?
FAQ ID -2731
How can I identify the binding sites of my transcription factor using ChIP?
FAQ ID -2753
How can I normalize my EpiTect ChIP results?
FAQ ID -2751
What is the recommended amount of input template for each RT² qPCR Primer Assay?
FAQ ID -2716
What is the RT² Profiler PCR Array?
FAQ ID -2718
Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples?
FAQ ID -2732
Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays?
FAQ ID -2765
Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region?
FAQ ID -2744
Can I manually set the threshold line?
FAQ ID -2702
Can I use your EpiTect One-Day kit with transcription factors?
FAQ ID -2752
Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples?
FAQ ID -2733
Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased?
FAQ ID -2709
Are there any unique elements that need to be incorporated into an shRNA expression vector?
FAQ ID -2772
Can I use total RNA for the miRNA PCR Arrays or Assays?
FAQ ID -2726
At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
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