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PCR/qPCR/dPCR

믿고 사용할 수 있는 특이적, 민감하며 견고한 PCR.

유전자 증폭을 수행할 때 실험의 성공 여부는 처리량, 분석 민감도, 까다로운 템플릿에서도 견고한 반응, 신뢰할 수 있는 데이터 정규화 등 몇 가지 중요한 요소에 따라 달라질 수 있습니다. 또한 사용 중인 PCR 방식과 관련된 특정 문제를 극복해야 할 수도 있습니다.

어떤 방식을 선택하시든, 당사는 end-point PCR, qPCR, dPCR에 최적화된 다양한 솔루션을 제공합니다. 당사의 키트, 분석, 장비, 부속품은 자신 있게 표적을 증폭 및 검출하고 거의 모든 응용 분야에서 워크플로우를 간소화할 수 있도록 도와줍니다.

  • Measure gene expression (mRNA, miRNA)
  • Quantify DNA or RNA targets
  • Detect & quantify pathogen and/or viral RNA/DNA
  • Quantify residual DNA
  • Convert RNA to cDNA for quantification
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  • Mutation detection (including rare/ultra-rare variants)
  • Analyze copy number variation (CNV)
  • Detect viral load
  • Gene expression quantification
  • Quantify low-abundance targets
  • Measure residual DNA
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  • Amplify DNA for cloning or sequencing
  • Qualitative pathogen detection (presence/absence)
  • CRISPR screening
  • Genotyping
  • Convert RNA to cDNA for storage
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  • Cq/Ct values
  • Relative quantification (ΔΔCq) or absolute quantification from standards
  • cDNA for downstream use
  • Absolute target quantification (copies/µL reaction)
  • Fractional abundance (%)
  • Mutation/target frequency
  • Copy number per genome
  • Amplified DNA or cDNA product
  • Band visualization on gel
  • Presence/absence result
  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
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  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Purified samples recommended
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  • Isolated DNA or cDNA
  • Isolated RNA
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  • Quantitative real-time PCR
  • Reverse transcription
  • Amplification and fluorescence-based detection in real-time for quantification
  • Quantitative digital PCR
  • Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
  • Standard end-point PCR
  • DNA amplification and visualization (e.g., gel-based) and downstream applications
  • When reliable quantification is required over a wide linear range
  • When standard curve-based or relative quantification is sufficient
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  • When precise quantification is required
  • When detecting rare alleles or small fold changes
  • When standard curves are undesirable
  • When working near the detection limit (low-abundance targets)
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  • When qualitative detection without quantification is sufficient
  • When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
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  • Real-time PCR thermal cycler
  • Dye-based (e.g., SYBR Green) or probe-based detection
  • QIAcuity dPCR platform
  • Probe‑based or intercalating dye detection
  • Thermal cycler
  • Visualization (e.g., via agarose gel electrophoresis)
검색 PCR/qPCR/dPCR

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DNA methylation analysis
156 개의 찾은 제품
QuantiTect RT-PCR Kits
QuantiTect RT-PCR Kits
유전자 발현 분석을 위해 SYBR® Green I을 사용한 원스텝 qRT-PCR 및 서열 특이적 프로브를 사용한 멀티플렉스 qRT-PCR 및 원스텝 qRT-PCR에 사용
RT2 SYBR® Green qPCR Mastermixes
RT2 SYBR® Green qPCR Mastermixes
For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
HotStarTaq Master Mix Kit
HotStarTaq Master Mix Kit
High PCR specificity를 위한 Premixed solution
QuantiTect PCR Kits
QuantiTect PCR Kits
SYBR Green I을 사용한 qPCR 및 투스텝 qRT-PCR용 및 qPCR 및 투스텝 qRT-PCR용, 또한 서열 특이적 프로브를 사용한 멀티플렉스 qPCR 및 투스텝 멀티플렉스 qRT-PCR용
QuantiFast Pathogen +IC Kits
QuantiFast Pathogen +IC Kits
바이러스 RNA/DNA와 박테리아 DNA의 안정적인 고감도 검출용, 내부 대조물질 포함
HotStarTaq Plus DNA Polymerase
HotStarTaq Plus DNA Polymerase
별도로 조건을 setup할 필요없이 특이성이 높은 hot-start PCR을 수행할 수 있습니다
miRCURY LNA SYBR® Green PCR Kits
miRCURY LNA SYBR® Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Taq DNA Polymerase
Taq DNA Polymerase
For standard and specialized PCR applications
QuantiTect Primer Assays
QuantiTect Primer Assays
For use in 2-step and 1-step qRT-PCR with SYBR® Green detection for gene expression analysis
miRCURY LNA miRNA PCR Assays
miRCURY LNA miRNA PCR Assays
LNA에 최적화된 SYBR® Green 기반 miRNA PCR을 사용하는 매우 민감하고 특이적인 miRNA 정량화용
miRCURY PCR Controls
miRCURY PCR Controls
Validated primer sets for normalization of miRNA expression levels in real-time PCR analyses
RT2 First Strand Kit
RT2 First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays