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PCR/qPCR/dPCR

믿고 사용할 수 있는 특이적, 민감하며 견고한 PCR.

유전자 증폭을 수행할 때 실험의 성공 여부는 처리량, 분석 민감도, 까다로운 템플릿에서도 견고한 반응, 신뢰할 수 있는 데이터 정규화 등 몇 가지 중요한 요소에 따라 달라질 수 있습니다. 또한 사용 중인 PCR 방식과 관련된 특정 문제를 극복해야 할 수도 있습니다.

어떤 방식을 선택하시든, 당사는 end-point PCR, qPCR, dPCR에 최적화된 다양한 솔루션을 제공합니다. 당사의 키트, 분석, 장비, 부속품은 자신 있게 표적을 증폭 및 검출하고 거의 모든 응용 분야에서 워크플로우를 간소화할 수 있도록 도와줍니다.

  • Measure gene expression (mRNA, miRNA)
  • Quantify DNA or RNA targets
  • Detect & quantify pathogen and/or viral RNA/DNA
  • Quantify residual DNA
  • Convert RNA to cDNA for quantification
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  • Mutation detection (including rare/ultra-rare variants)
  • Analyze copy number variation (CNV)
  • Detect viral load
  • Gene expression quantification
  • Quantify low-abundance targets
  • Measure residual DNA
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  • Amplify DNA for cloning or sequencing
  • Qualitative pathogen detection (presence/absence)
  • CRISPR screening
  • Genotyping
  • Convert RNA to cDNA for storage
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  • Cq/Ct values
  • Relative quantification (ΔΔCq) or absolute quantification from standards
  • cDNA for downstream use
  • Absolute target quantification (copies/µL reaction)
  • Fractional abundance (%)
  • Mutation/target frequency
  • Copy number per genome
  • Amplified DNA or cDNA product
  • Band visualization on gel
  • Presence/absence result
  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
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  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Purified samples recommended
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  • Isolated DNA or cDNA
  • Isolated RNA
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  • Quantitative real-time PCR
  • Reverse transcription
  • Amplification and fluorescence-based detection in real-time for quantification
  • Quantitative digital PCR
  • Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
  • Standard end-point PCR
  • DNA amplification and visualization (e.g., gel-based) and downstream applications
  • When reliable quantification is required over a wide linear range
  • When standard curve-based or relative quantification is sufficient
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  • When precise quantification is required
  • When detecting rare alleles or small fold changes
  • When standard curves are undesirable
  • When working near the detection limit (low-abundance targets)
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  • When qualitative detection without quantification is sufficient
  • When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
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  • Real-time PCR thermal cycler
  • Dye-based (e.g., SYBR Green) or probe-based detection
  • QIAcuity dPCR platform
  • Probe‑based or intercalating dye detection
  • Thermal cycler
  • Visualization (e.g., via agarose gel electrophoresis)
검색 PCR/qPCR/dPCR

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DNA methylation analysis
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