Disposable probes를 이용하여 low-throughput으로 샘플을 균일하게 파쇄할 수 있습니다
Products
TissueRuptor은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
TissueRuptor Disposable Probes (25)
Cat. No. / ID: 990890
25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)
TissueRuptor II (120 V, 60 Hz, US)
Cat. No. / ID: 9002755
Handheld rotor–stator homogenizer, 120 V, 60 Hz (for North America), 5 TissueRuptor Disposable Probes
Features
- 각각의 샘플마다 새로운 probe를 사용함으로써 cross-contamination제거되는 일회용 probe 사용
- 빠르고 효과적인 파쇄 — 다양한 sample type에서의 안정적인 파쇄
- 시간 절감 효과 — 각각의 sample을 파쇄한 후, 일회용 probe는 버림
- 최적화된 protocols — 검증된 QIAGEN 정제기술 통합
- Flexibility — 여러가지 샘플 형태, lysis buffer, 파쇄용기를 자유롭게 사용가능
Product Details
The TissueRuptor is a handheld rotor-stator homogenizer that provides rapid, efficient, and flexible disruption of human, animal, and plant samples for a wide range of downstream applications. The TissueRuptor uses transparent disposable probes, which helps to minimize the risk of cross-contamination and enables visual control of the sample disruption process. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample.
Performance
QIAGEN has tested use of the TissueRuptor in combination with proven QIAGEN sample preparation kits. Sample disruption with the TissueRuptor ensures maximal yields of nucleic acids and proteins that perform well in downstream applications such as PCR, western blotting, and enzyme assays (see figures "Successful purification of phosphoprotein " and " High performance results in PCR"). For most tissues, disruption and homogenization using the TissueRuptor gives comparable results to traditional rotor–stator homogenization (see figure "Pure RNA with high RIN values"). Effective disruption of tissue samples using the TissueRuptor allows reproducible purification of high-quality DNA and RNA using QIAGEN nucleic acid purification kits (see figures "Pure RNA with high RIN values", "Reproducible purification of high-quality genomic DNA", and "Efficient disruption and homogenization of animal tissues").
See figures
Principle
Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueRuptor system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).
The TissueRuptor is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.
QIAGEN also provides RNAlater RNA Stabilization Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor.
| Analyte purified | Sample type | QIAGEN kit |
|---|---|---|
| RNA | Easy-to-lyse tissue | RNeasy Kits |
| RNeasy Plus Kits | ||
| RNeasy Protect Kits | ||
| RNA | Fiber-rich tissue | RNeasy Fibrous Tissue Kits |
| RNA | All types of tissue | RNeasy Lipid Tissue Kits |
| RNeasy Universal Tissue Kits | ||
| RNA | Plant tissue | RNeasy Plant Mini Kit |
| RNA | Yeast | RNeasy Kits |
| RNA | Bacteria | RNeasy Protect Bacteria Kits |
| microRNA | All types of tissue | miRNeasy Kits |
| DNA | Human tissue | QIAamp DNA Kits |
| DNA | Animal tissue | DNeasy Blood & Tissue Kits |
| Protein | Tissue | Qproteome Mammalian Protein Prep Kit |
| Phosphoprotein | Tissue | PhosphoProtein Purification Kit |
| Glycoprotein | Tissue | Qproteome Glycoprotein Kits |
| DNA and RNA | Tissue | AllPrep DNA/RNA Kits |
| DNA, RNA, and protein | Tissue | AllPrep DNA/RNA/Protein Mini Kit |
Procedure
The TissueRuptor is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal, and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.
After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.
Applications
The TissueRuptor enables efficient disruption and homogenization of human, plant, and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids, or protein can then be performed using QIAGEN kits.
Supporting data and figures
Reproducible purification of high-quality genomic DNA.
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.
Specifications
| Features | Specifications |
|---|---|
| Disruption principle | Simultaneous disruption and homogenization by rotation of the blade of the disposable probe |
| Technology | Handheld rotor-stator homogenizer |
| Kits compatible with instrument | All Kits for purification of RNA/DNA/protein |
| Technical data | AUS/EU/UK: 230 V, 144 W, 50/60 Hz, 5000-35,000 rpm; NA: 120 V, 144 W, 50/60 Hz, 5000-35,000 rpm; Japan: 100 V, 125 W, 50/60 Hz, 5000-28,000 rpm |
| Typical run time | 15–120 seconds |
| Features | Rapid, effective disruption of a range of sample types. Disposable probes minimize risk of cross-contamination. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies. |
| Throughput | 1 sample per run |
Resources
Brochures & Guides (1)
Supplementary Protocols (3)
User-Developed Protocols (5)
Kit Handbooks (1)
Safety Data Sheets (1)
Gene Expression Analysis (1)
Instrument User Manuals (1)
Certificates of Analysis (1)
FAQ
2. Can the TissueRuptor stainless steel probe be autoclaved?
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?
What is the minimum liquid volume that should be used with the TissueRuptor
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
Can I use Buffer ATL for disruption of my samples with TissueRuptor, TissueLyser II, or TissueLyser LT instruments?
3. Can the TissueRuptor instrument be autoclaved?
Can the TissueRuptor Disposable Probes be autoclaved?
Can the disposable probes be re-used? How should they be washed?
What is the catalog number for steel probes and disposable probes for use with QIAGEN’s TissueRuptor instrument? What are the dimensions of these probes?