MaXtract High Density

유기 용제에서 더 안전하고 편리한 핵산 추출용

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MaXtract High Density (200 x 2 ml)

Cat. No. / ID: 129056

200 x 2ml MaXtract High Density 튜브

US$294.00

Quantity

200 x 2 ml

200 x 1.5 ml

100 x 15 ml

25 x 50 ml

Quantity

MaXtract High Density은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

유기 용제에서 더 안전한 핵산 추출
오염 물질 캐리오버 감소
높은 핵산 회수율
편리한 핵산 회수

Product Details

MaXtract High Density는 유기 용제(예: 페놀/클로로포름)에서 핵산 추출을 간소화합니다. MaXtract 겔은 유기 용제와 핵산 함유 수상 사이에 안정적인 벽을 형성하여, 유기 솔루션, 단백질 및 기타 오염 물질의 캐리오버를 방지하면서도 쉽게 수상을 회수할 수 있도록 합니다.

Performance

MaXtract High Density를 사용하면 전통적인 추출 방법 대비 30% 더 높은 회수율을 달성할 수 있습니다. 단백질과 기타 오염 물질은 유기상 및 중간상으로 이동합니다. MaXtract High Density 겔로 형성된 벽은 이러한 오염 물질이 벽 아래에 계속 갇히도록 하여 수상을 제거할 때 캐리오버를 방지할 만큼 충분히 안정적입니다. 수상은 디캔팅(decanting)이나 피펫팅(pipetting)으로 제거할 수 있으며, 전통적인 유기 추출 방법 대비 최대 30% 더 높은 핵산 회수율을 보입니다.

MaXtract High Density는 다양한 튜브 사이즈로 제공되어 100µl~20ml 범위의 샘플 용량에서 추출이 가능합니다.

MaXtract High Density를 이용한 상 분리
수상	페놀:클로로포름	클로로포름
<0.5M NaCl, <1mg/ml 단백질	예	예
≥0.5M NaCl	예	예
≥1Mg/ml 단백질	예	예
플라스미드 DNA 분리	예	예
유전체 DNA 분리	예	예
RNA 분리	예	예

* 페놀:클로로포름:이소아밀 알코올 포함.

Principle

MaXtract High Density 겔은 페놀/클로로포름과 같은 용매를 사용하여 유기 추출을 통해 안전하고 빠르게 핵산을 회수합니다(흐름도 '' 참조).

See figures

MaXtract 절차.

Procedure

핵산 용액과 유기 용제를 MaXtract 겔이 담긴 튜브에 첨가하기만 하면 됩니다. 혼합 및 원심분리 이후에 MaXtract High Density 겔은 안정적인 벽을 형성하여 유기상 및 수상을 분리합니다. 이 벽은 안전하게 위험 유기 용제와 오염 물질을 가두어, 핵산 함유 수상을 쉽게 새로운 튜브로 디캔팅(decanting)하거나 피펫팅(pipetting)할 수 있도록 합니다(그림 ' 안전하고 쉬운 핵산 추출' 참조). 두 번째 추출이 필요한 경우, 최대 튜브 용량을 초과하지 않는다면 동일한 튜브에서 수행할 수 있습니다.

MaXtract High Density 겔을 사용한 분리는 수성 매체와 유기성 매체 사이의 밀도 차이에 의존합니다. 유기층은 겔과 수층보다 밀도가 더 높아야 하며, 겔은 수상보다 밀도가 더 높아야 합니다.

See figures

안전하고 쉬운 핵산 추출.

Applications

MaXtract High Density는 플라스미드 DNA, 유전체 DNA 또는 RNA 분리에 매우 적합합니다. 저용해점 아가로스에서 DNA 회수 등 특정 애플리케이션에 최적화된 프로토콜 또한 해당됩니다. MaXtract High Density 겔을 사용하여 추출한 핵산은 여러 다운스트림 애플리케이션에 적합합니다.

Supporting data and figures

MaXtract 절차.

MaXtract 절차는 신뢰성과 편리성을 제공합니다.

Resources

Download Safety Data Sheets for QIAGEN product components.

For improved recovery of nucleic acids during organic extraction procedures

FAQ

Yes. The organic phase can be placed in MaXtract Low and High Density tubes without any problem 3-4 hours before preparation of the samples. Make sure to perform the centrifugation step first, so that the gel collects at the bottom of the tube.

FAQ ID -1299

No. After autoclaving, the MaXtract gel no longer has the same properties. Since the MaXtract tubes are manufactured and filled in a fully automated process, autoclaving is not required.

FAQ ID -1305

DNA purified by organic extraction can principally be used for these downstream reactions. It should be noted that phenol, like all organic solvents, alters the active centre of enzymes, thus deactivating them. An ethanol precipitation should therefore generally be carried out prior to enzyme controlled downstream reactions. Potential phenol residue is thereby effectively removed.

The use of MaXtract Low and High Density tubes has the advantage that no recontamination of the sample by organic solvents or proteins occurs.

FAQ ID -1303

Generally, this is determined by the density of the denatured proteins and the gel type in the MaXtract Tubes. If proteins show a higher density than the gel, they will accumulate underneath in the organic phase. If they have the same density as the MaXtract gel, they will collect in the gel. The quantity and ratio of phenol:chloroform can also influence the behavior of the proteins.

FAQ ID -1312

Yes, we have the following protocols:

Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).

FAQ ID -1550

Yes, this application is possible with both MaXtract Low and High Density Tubes. A sufficient quantity of BCP should be used when using MaXtract High Density, in order to achieve a stable gel phase. BCP increases the density of the organic phase in the same way as chloroform.

-3

No. The functional efficiency of the MaXtract Low and High Density Tubes does not depend on the pipetting sequence.

FAQ ID -1307

If organic solvents are added immediately following the filling of MaXtract Low and High Density Tubes with the frozen sample, it should not have any negative effect on the functionality of the tubes.

FAQ ID -1301

Yes. When using MaXtract Tubes with QIAzol Lysis Reagent, please follow the User-Developed protocol 'Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density' (RY31) .

FAQ ID -1304

MaXtract High Density gel is found in the yellow tubes, and MaXtract Low Density gel is contained in the green tubes.

FAQ ID -1315

Yes, if a sufficiently large MaXtract tube has been selected for the extraction it is possible to perform multiple purification steps in this tube. Sample and organic solvent are placed in the MaXtract tube, mixed and centrifuged. Organic solvent is then added again to the aqueous phase separated by the gel, followed by mixing and centrifuging. Following the last extraction step, the sample is transferred to a new tube for storage.

For the purification of samples with high protein content, we recommend using a new MaXtract tube for each extraction step.

FAQ ID -1302

CTAB as a rule increases the density of the aqueous phase. Therefore, MaXtract High Density should be used for the organic extraction of DNA from CTAB samples.

Depending on the amount of CTAB used, the density of the sample can become too high for the MaXtract High Density Tube, leading to a gel barrier above the aqueous phase. In this case, puncture the gel with the tip of a pipette and dilute the aqueous phase with either distilled water or TE buffer. Mix again and then centrifuge. However, we recommend to repeat the separation in a new MaXtract tube.

FAQ ID -1308

A phenol-chloroform ratio of 1:1 is advantageous for the phase separation when using MaXtract Low and High Density, since the MaXtract interphase has the greatest stability at this ratio.

Methods using a phenol-chloroform ratio of as much as 6:1 are also possible with MaXtract. However, MaXtract can partly precipitate onto the bottom of the tube in this case.

If the phenol-chloroform ratio is greater than 1:1, it is necessary to use MaXtract Low Density Tubes. Note that this MaXtract type is not compatible with applications in which the aqueous phase has a high density (e.g., isolation of plasmid DNA and RNA).

FAQ ID -1309

In this case, both MaXtract Low and High Density Tubes can be used with the same success. We principally recommend testing both MaXtract types.

FAQ ID -1310

No, MaXtract Low and High Density tubes are not coated in any way.

FAQ ID -1298