Deep dive into OEM custom enzymes and bulk manufacturing capabilities
Assay developers are challenged with consolidating the supply chain, while ensuring top-quality components for a consistent end-product. OEM by QIAGEN can help simplify this process by providing a wide range of enzymes in bulk. Custom enzyme manufacturing can be the key to successfully bringing a commercial assay to market. A dedicated project manager will work with you from the start. We can tailor enzymes to your specifications, including sampling and testing. Check out our broad enzyme portfolio. Manufacturing quantities can grow with your business. Allow us to be your trusted partner for OEM enzymes.
Enzyme Portfolio Overview
DNA Polymerases
| Product name |
VeraSeq 2.0 High Fidelity DNA Polymerase |
VeraSeq Ultra DNA Polymerase |
Phoenix Hot-Start Taq DNA Polymerase |
Taq DSC 2.0 DNA Polymerase |
|---|---|---|---|---|
| Description |
For maximized DNA amplification speed, accuracy, and length |
To read and use Uracil residue with maximized speed and accuracy |
For enhanced specificity, sensitivity, and yield |
A nucleic acid based hot
start polymerase with instant activation |
| Features |
• 1kB in 15 secs. • Fidelity 50x higher than Taq • Blunt end • 1.5-3.0mM Mg2+ concentrations |
• 1kB in 15 secs. • Fidelity 25x higher than Taq • Blunt end • 1.5-3.0mM Mg2+ concentrations |
• Reduced nonspecific amplification and primer-dimer formation • Room temperature reaction set up concentrations |
• Thermostable, processive DNA polymerase • No 3' to 5' proofreading • Hot start |
| Applications |
Routine HiFi amplification, NGS library amplification and synthetic biology |
Bisulfite-Seq,
contamination prevention, long-range |
Routine PCR, qPCR, multiplexing, RT-PCR |
Routine PCR, qPCR, multiplexing, RT-PCR |
| Exonuclease activity |
3'➞5' | 3'➞5' | 5'➞3' | 5'➞3' |
| Hot-start | ✔ | ✔ | Antibody based | Nucleic acid based |
| Thermostable | Ultra-thermostable | Ultra-thermostable | ✔ | ✔ |
| Proof reading | ✔ | ✔ | ||
| High fidelity | ✔ | ✔ | ||
| Long range | ✔ | ✔ | ||
| Lyo-ready, glycerol-free |
✔ | ✔ |
| Product name | Taq-B DNA Polymerase | TaqIT DNA Polymerase | Phi29 DNA Polymerase | BstX DNA Polymerase |
|---|---|---|---|---|
| Description | Wild-type Taq DNA polymerase |
Exonuclease deficient derivative of Taq DNA polymerase |
Highly processive DNA polymerase, for accuracy and speed |
Thermostable DNA polymerase for better isothermal amplification |
| Features |
• 5' to 3' exonuclease activity • 5' to 3' nick translation moiety No 3' to 5' proofreading |
• Slightly greater fidelity, inhibition resistance and thermostability than full length Taq |
• Strong strand displacement activity and processivity up to 70,000 base insertions per binding event |
• Strong strand displacement • High salt and detergent tolerance |
| Applications | Industry standard for routine PCR |
Longer PCR amplification, genotyping, allows bypassing of DNA extraction and purification steps |
Whole Genome Amplification (WGA), Rolling Circle Amplification (RCA), Multiple Displacement Amplification (MDA) |
LAMP, strand-displacement Synthesis ideal for isothermal amplification |
| Exonuclease activity |
5'➞3' | 3'➞5' | ||
| Hot-start | ✔ | |||
| Thermostable | ✔ | High thermostability | ✔ | |
| Proof reading | ✔ | |||
| High fidelity | ✔ | |||
| Long range | ✔ | ✔ | ✔ | |
| Lyo-ready, glycerol-free |
✔ | ✔ | ✔ | ✔ |
| Enzyme category | Binding Protein | Other | Other |
|---|---|---|---|
| Product name | T4 gene 32 Protein | Uracil DNA Glycosylase | 10x Uracil Cleavage System |
| Description | Wild-type For ssDNA stabilization and increased processivity |
Uracil DNA glycosylase for removing Uracil from DNA |
Uracil DNA glycosylase (UDG) and Endonuclease VIII |
| Features | • A single-stranded DNA binding protein |
• Catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar |
• Removal of uracil, then cleavage of DNA backbone |
| Applications | DNA sequencing in secondary structure rich regions and PCR amplification |
Creating a basic sites, containing single or double-stranded DNA |
Cloning, removing contaminating PCR products |
| Exonuclease activity |
✔ | ||
| Lyo-ready, glycerol-free |
✔ | ✔ |
RNA Polymerases & RT
| Product name | Poly(A) Polymerase | T7 RNA Polymerase |
|---|---|---|
| Description | Catalyzes addition of AMP from ATP to the 3" hydroxyl of RNA | Synthesizes RNA in 5'➞3' direction of DNA template |
| Features | • Template independent catalysis of the addition of AMP from ATP to the 3' hydroxyl of RNA |
• DNA-dependent RNA polymerase with high specificity to T7 promoter |
| Applications | Addition of poly(A) tail to RNA, 3'-end RNA labeling |
RNA synthesis from DNA template, RNA probe preparation, applications in mRNA therapeutics |
Reverse Transcriptase
| Product name | Omniscript RT | Sensiscript |
EnzScript (MMLV Reverse Transcriptase RNase, H-) |
RNase inhibitor | RNase H |
|---|---|---|---|---|---|
| Description | For viral RNA preparation with carrier use |
For highly sensitive applications with very small amounts of RNA (<50 ng) |
RNA-dependent DNA polymerase |
Porcine-derived non-competitive RNase A, B, C inhibitor |
Cleaves RNA strand of DNA-RNA hybrid |
| Features |
• Efficient and sensitive RT with high cDNA yields from small amounts of RNA |
• Efficient and sensitive RT • For use with less than 50 ng of RNA |
• No RNase H activity • cDNA transcripts > 5kb • Reaction |
• Does not inhibit RNase H activity |
• Degrades the RNA strand of RNA-DNA hybrid • Does not degrade single or double stranded RNA |
| Applications |
cDNA synthesis, one-step RT-PCR |
Single-cell RT-PCR, small biopsies analysis, cDNA synthesis, one-step RT-PCR |
cDNA synthesis, one-step RT-PCR, RNAseq |
cDNA synthesis, one-step RT-PCR |
Removes mRNA during second strand cDNA synthesis |
| Long range | ✔ | ||||
| Lyo-ready, glycerol-free |
✔ | ✔ | ✔ |
Modifying Enzymes
DNA modifying enzymes
| Product name | DNA Polymerase I | Klenow Fragment |
Klenow (3'-5'exo-) Fragment |
Mako DNA Polymerase |
T4 DNA Polymerase |
T7 DNA Polymerase |
|---|---|---|---|---|---|---|
| Description | Mesophilic DNA polymerase |
Truncated product of E. coli DNA polymerase I |
A derivative of E. coli DNA polymerase I |
Exonuclease- deficient polymerase |
Extension of a primed DNA template in 5'➞3' direction |
Highly processive DNA polymerase |
| Features |
• 5'➞3' DNA synthesis |
• 5'➞3' polymerase |
• 5'➞3' polymerase |
• No strand displacement activity |
• Polishing 5' and 3' of ends • No strand displacement |
• Exceptionally high synthesis and replication fidelity rate |
| Applications |
DNA labeling by nick translation, cDNA synthesis |
DNA blunting by fill-in 5’ overhang, second strand cDNA synthesis, sequencing and site-specific mutagenesis |
A-tailing for NextGen sequencing, strand displacement amplification, DNA labeling |
Sequencing |
Double-stranded DNA labeling |
Highly processive, site-specific mutagenesis, second strand cDNA synthesis |
| Exonuclease activity |
Both 3'➞5' and 5'➞3' exonuclease activity |
3'➞5' exonuclease activity, no 5'➞3' exonuclease activity |
Deficient in proofreading (3'➞5') and nick-translation (5'➞3') nuclease activities |
No 3'➞5' or 5'➞3' exonuclease activity |
Powerful 3'➞5' exonuclease activity, no 5'➞3' exonuclease activity |
3'➞5' exonuclease activity |
| Lyo-ready, glycerol-free |
✔ |
✔ |
✔ | ✔ |
| Product name |
T4 Polynucleotide Kinase |
Terminal Deoxynucleotidyl Transferase |
Thermostable Pyrophosphatase |
End Repair Mix | E. coli fpg | Endonuclease VIII |
|---|---|---|---|---|---|---|
| Description | 5' phosphorylation of DNA or RNA, removes 3' phosphoryl groups |
A template- independent DNA polymerase |
Catalyzes the hydrolysis of inorganic pyrophosphate to produce orthophosphate |
Combination of T4 DNA Polymerase and T4 PNK |
Participates in the base-excision pathway of DNA repair enzymes |
DNA repair protein, removes modified pyrimidines |
| Features | • Exhibits 3'-phosphatase and 2', 3' cyclic phosphodiesterase activities |
• Catalyzes deoxynucleotides addition to the 3' hydroxyl terminus of single or double stranded DNA |
• Eliminates pyrophosphate during amplification, thus minimizing inhibition • Functional under PCR conditions |
• Converts DNA with 5'- and/or 3'-protruding ends to 5'- phosphorylated, blunt-ended DNA. (in high/low concentrations) |
• Acts both as a N-glycosylase and an AP-lyase |
• Possesses both DNA glycosylase and apurinic/ apyrimidinic (AP) lyase activities |
| Applications | Phosphorylation of 5' ends of DNA prior to ligation |
Homopolymeric tailing to the 3'- OH, TUNEL assay, 5’-RACE, labeling of 3’-end |
In-vitro synthesis |
Generate blunt-ended DNA for cloning or NGS library preparation |
Excises damaged purines from duplex DNA, cleaves AP sites leaving 3' and 5' phosphates |
Excises damaged pyrimidines from duplex DNA, cleaves AP sites leaving 3' and 5' phosphates |
| Thermostable | ✔ | |||||
| Lyo-ready, glycerol-free |
✔ | ✔ | ||||
| NGS applications | ✔ |
NGS library preparation
| Product name | 5X WGS Fragmentation Mix | 5X ER/A-Tailing Enzyme Mix |
|---|---|---|
| Description | A single tube solution for library construction on Illumina platforms |
A NGS library preparation module |
| Features | • Fragmentation, end-repair and dA-tailing in a single reaction step • Ligation optional in the same tube • Profiles similar to mechanical shearing Modifiable fragment size • Input DNA from 1ng to 1ug, with various GC contents |
• End-repair and dA-tailing in a single reaction step • Input DNA from 250 pg to 1 ug • Compatible with EDTA • Total time under 3 hours/1 hour hands-on time • Even coverage across genomic regions • PCR free library prep |
| NGS Illumina library construction |
From intact DNA | From fragmented DNA |
| Lyo-ready, glycerol-free | Feasible | |
| NGS applications | ✔ | ✔ |
Additional modifications
| Product name | Restriction enzymes | Exonucleases |
|---|---|---|
| Description | Example Products: BamHI, BglII, DpnI, EagI, EcoRI, EcoRV,
HaeIII, HindIII, NcoI, NotI, PstI, PvuII, SalI, SphI, XbaI, XhoI |
Example Products in the exonuclease family are: Exonuclease
I, III and Lambda exonuclease |
| Features | • Cleaves double stranded DNA within target recognition sites | • Exonuclease I cleaves single-stranded DNA in the 3'➞5' direction • Exonuclease III acts in 3’-5’ direction on double stranded DNA substrate • Lambda Exonuclease is a highly processive 5'➞3' double stranded exonuclease |
| Applications | Endonuclease application: cloning and restriction site mapping | Removal of chromosomal DNA to clean up plasmid preps, generation of ssDNA from linear dsDNA, removal of oligonucleotide primers post PCR |
Ligases
| Product name | WGS Ligase | T3 DNA Ligase | T7 DNA Ligase | E. coli DNA Ligase | T4 DNA Ligase |
|---|---|---|---|---|---|
| Description | Optimized for ligation following WGS Fragmentation |
ATP-dependent dsDNA ligase. Catalyzes the formation of a phosphodiester bond between a 5' phosphate and a 3' hydroxyl termini in duplex DNA |
Catalyzes the formation of a phosphodiester bond between a 5' phosphate and a 3' hydroxyl termini in duplex DNA |
An NAD+-dependent enzyme |
Catalyzes the formation of phosphodiester bond between the terminal 5’ phosphate and 3’ hydroxyl groups of duplex DNA or RNA |
| Features | • Joins blunt end and cohesive ends and • Repairs single stranded nicks in duplex RNA, RNA, or DNA-RNA hybrid |
• Joins blunt end and cohesive ends and repairs single stranded nicks in duplex DNA |
• Joins blunt end and cohesive ends and repairs single stranded nicks in duplex DNA |
• Ligates DNA at nicks and cohesive termini |
• Joins blunt end and cohesive ends and • Seals nicks in duplex DNA, RNA or DNA-RNA hybrid |
| Applications |
NGS library construction, cloning |
dsDNA ligase that works in high ionic strength up to 1.0 M NaCl |
High cohesive end ligation efficiency |
cDNA cloning by replacement synthesis |
Restriction cloning, TA cloning, adapter ligation |
| Lyo-ready, glycerol-free lyophilized |
Feasible | ✔ |
| Product name | Taq DNA Ligase | T4 RNA Ligase 1 | T4 RNA Ligase 2 | T4 RNA Ligase 2 Truncated |
|---|---|---|---|---|
| Description | Thermostable DNA ligase | Ligates single-stranded RNA and single stranded DNA molecules |
Ligates nicks on double stranded DNA |
Ligates pre-adenylated 5' phosphate DNA or RNA and 3' hydroxyl of RNA |
| Features |
• Seals nicks and discriminates against mismatch ligation |
• Catalyzes ATP-dependent ligation of single-stranded nucleic acids |
• Ligates from 3' OH of RNA to 5' phosphate of DNA in double stranded structures |
• Linker ligations with pre-adenylated 5' DNA to 3' hydroxyl RNA |
| Applications | Ligase Chain Reaction and Ligase Detection Reaction, high temperature ligation |
Single-stranded nucleic acid (RNA or DNA) ligation, labeling of 3'-termini of RNA |
Nick ligation in dsRNA, Ligate the 3' OH of RNA to the 5' phosphate of DNA in a double stranded format |
NGS RNA library construction (miRNA-Seq or directional mRNA-Seq) |
| Thermostable | ✔ | |||
| High fidelity | ✔ |
PCR master mixes
| Product name | QuantiNova Multiplex PCR Kit | QuantiNova Probe PCR Kit | QuantiTect Probe PCR Kit | QuantiTect Multiplex PCR Kit |
|---|---|---|---|---|
| Description | Ultrafast multiplex, real-time PCR and two-step qRT-PCR using sequence-specific probes |
Highly sensitive, specific, and ultrafast PCR mastermix for probe-based real-time PCR |
Chemically inactivated hot-start PCR mastermix for real-time PCR using probe-based detection |
Probe-based detection |
| Features | • Hot-start • Sensitive detection of up to five targets in one tube • Visual pipetting control • Room temperature reaction set up |
• Hot-start • Detection of rare targets down to one copy • Visual pipetting control • Room temperature reaction set up |
• Hot-start • Very high PCR specificity • dUTP for pretreatment with uracil-N-glycosylase (UNG) |
• Hot-start • Very high PCR specificity • Multiplex capability up to five targets • dUTP for pretreatment with uracil-N-glycosylase (UNG) |
| Applications | Multiplex gene expression analysis of cDNA or gDNA targets on any real-time cycler |
Probe-based gene expression analysis of cDNA targets and quantitative gDNA analysis on any real-time cycler |
Real-time PCR of cDNA or gDNA targets with duplex capability |
Multiplex real-time PCR |
| Hot-start | Antibody mediated | Antibody mediated | Chemical modification | Chemical modification |
| Real-time PCR | ✔ | ✔ | ✔ | ✔ |
| Multiplex PCR | ✔ | ✔ | ✔ | ✔ |
| Product name | UltraRun LongRange PCR Kit | AllTaq Master Mix Kit | HiFi PCR Master Mix |
|---|---|---|---|
| Description | Accurate ultra-long-range PCR | Sensitive and specific Hot-start PCR | High fidelity PCR amplification of NGS libraries |
| Features | • Up to 40 kb • Hot-start • Low error rates, ensured by high-fidelity enzyme |
• Room temperature stability • One protocol for all targets • GC-rich or up to 9 kb • Duplex PCR • Visual pipetting control • Gel tracking dyes |
• Highly efficient and low bias PCR master mix • Uniform coverage across challenging AT- and GC-rich regions • Input DNA ≥250 pg |
| Applications | Cloning, sequencing, standard PCR |
PCR, genotyping and genetic analysis with multiple primer pairs or wide range of amplicon sizes |
DNA or RNA seq library amplification, including single cell RNAseq |
| Hot-start | Antibody mediated | Antibody mediated | Antibody mediated |
| End-point PCR | ✔ | ✔ | ✔ |
| Multiplex PCR | ✔ | ✔ | |
| Long range | ✔ | ||
| Contains HiFi in enzyme blend |
✔ | ✔ |
UltraClean® Production
| Product name | UCP Probe PCR Kit | UCP Multiplex PCR Kit | UCP HiFidelity PCR Kit |
|---|---|---|---|
| Description | Probe-based, UCP PCR | UCP Nucleic acid depleted PCR | High fidelity, hot-start UCP PCR |
| Features | • Nucleic acid-depleted reagents, no background from residual DNA. • Inhibitor robustness • Visual pipetting control • Room-temperature reaction setup |
• No background from residual DNA • Inhibitor robustness • Wide GC range • Visual pipetting control • Gel tracking dyes • Room temperature reaction setup |
• Blunt end products • Up to 10 kb • Inhibitor robustness for direct PCR from blood samples • Fidelity 70 times higher than Taq • Room temperature reaction setup |
| Applications | Microbial testing, microbiome/ metagenome workflows, NGS library preparation |
Microbial testing, quality control, genotyping and genetic testing, microbiome/metagenome analysis and sequencing, 16S/18S amplifications |
Microbial testing, quality control, genotyping and genetic testing, microbiome/metagenome analysis and sequencing, 16S/18S amplifications |
| Hot-start | Antibody mediated | Antibody mediated | Antibody mediated |
| Real-time PCR | ✔ | ||
| End-point PCR | ✔ | ✔ | |
| Multiplex PCR | ✔ | ✔ | ✔ |
| Ultra Clean Production (UCP) |
✔ | ✔ |
✔ |
| Room temperature reaction setup |
✔ | ✔ | ✔ |
| Inhibitor robustness |
✔ | ✔ | ✔ |
| Contains HiFi in enzyme blend |
✔ |
RT-PCR
| Product name | QuantiNova Pathoge + IC Kit |
QIAGEN One Step Ahead
RT-PCR kit |
QuantiNova Probe RT-PCR Kit |
QuantiNova Multiplex
RT-PCR Kit |
|---|---|---|---|---|
| Description | Real-time multiplex RT-PCR kit for probe-based pathogen detection |
For highly sensitive and specific RT-PCR using any RNA template |
For one-step qRT-PCR using sequence-specific probes for gene expression analysis |
Quantification of up to five RNA targets in a single tube by multiplex real-time RT-PCR |
| Features | • Ultrafast, simultaneous detection of pathogen DNA and RNA • Room temperature reaction setup • Visual pipetting control |
• One-step RT-PCR • Room-temperature setup • Duplex capability |
• Internal control for positive in-process verification • Duplex capability • Room temperature reaction setup • Visual pipetting control |
• Amplification from a single cell or up to 800 ng template • Internal control RNA for verification • Room temperature reaction setup • Visual pipetting control |
| Applications | 4-plex RT-PCR for detection of both pathogen targets and internal control |
RT-PCR applications for virus detection, gene expression analysis |
Gene expression analysis of RNA targets on any real-time cycler |
|
| Real-time PCR | ✔ | ✔ | ✔ | |
| End-point PCR | ✔ | |||
| Multiplex PCR | ✔ | |||
| Room temperature reaction setup |
✔ | ✔ | ✔ | ✔ |
| Hot-start | Antibody mediated hotstart Taq, inactivated RT |
Antibody mediated hotstart Taq, inactivated RT |
Antibody mediated hotstart Taq, inactivated RT |
Antibody mediated hotstart Taq, inactivated RT |
| Product name | QuantiTect Probe RT-PCR Kit | QuantiTect Multiplex RT-PCR Kit | ZipScript™ One-Step RT-qPCR Kit |
|---|---|---|---|
| Description | For one-step RT-qPCR using sequence-specific probes for gene expression analysis |
For RNA template using the SYBR® Green detection |
A highly sensitive and reproducible RT-qPCR solution optimized for real-time PCR |
| Features | • Sensitive detection of low-copy targets • Use of any sequence-specific probe on any real-time cycler • dUTP for pretreatment with uracil-N-glycosylase (UNG) |
• Omniscript/ Sensiscript mix for cDNA synthesis • dUTP for pretreatment with uracil-N-glycosylase (UNG) • Up to five targets in one reaction |
• The 25X enzyme mix is accompanied by a 2X reaction buffer • Capable of multiplexing up to five targets • Can be obtained lyophilized |
| Applications | Real-time quantification of RNA targets | One-step real-time PCR (1-step RT-qPCR) | Gene expression for RNA template, 1-step RT-qPCR |
| Real-time PCR | ✔ | ✔ | ✔ |
| Multiplex PCR | ✔ | ✔ | ✔ |
| Hot-start | Chemical modification | Chemical modification | Antibody mediated |
Additional OEM Capabilities
| Formulation capabilities | Consulting service |
|---|---|
| • Adjusted concentration • Lyophilized or in the buffer of your choice • Bulk or aliquots in tubes • Customized mixing • Tailored labeling and packaging, flexible volumetric ordering |
• Implement tailored modifications and design • Contract protein manufacturing • Stability studies • Custom mixes, buffer, and diluent support • R&D prototyping of proteins and formulations for ease of evaluation |
Interested in OEM? Contact us now
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