Custom & bulk formulations for industrial & commercial supply
For effective protein digestion in biological samples, DNA/RNA preparation kits, cartridges for automated devices and other molecular applications.
- Get all the details about Proteinase K and Proteinase K Ultrapure by checking out our Interactive Product Profile
Applications
Product details
Proteinase K is a recombinant subtilisin-related serine protease derived from the Parengyodontium album (Tritirachium album) that is active under a wide range of reaction conditions, including elevated temperatures and the presence of sodium dodecyl sulfate (SDS).
The enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product. An additional purification technology significantly increases solubility (2.5 fold), increases specific activity, and produces remarkable purity with DNA content ≤ 0.1 pg/mg for Proteinase K Ultrapure.
Proteinase K is free of exonucleases, endonucleases, and ribonucleases.
Assay | Proteinase K Powder and Solution Specification | Proteinase K Ultrapure Specification |
---|---|---|
Protein content | >70% | >70% |
Solubility in water | ≥20 mg/mL | ≥50 mg/mL |
Activity | ≥30 U/mg lyophilizate ≥40 U/mg protein ≥800 U/mL liquid |
≥35 U/mg lyophilizate ≥45 U/mg protein |
DNA content | ≤10 pg/mg ≤200 pg/mL |
≤0.1 pg/mg |
Single-stranded exonuclease | Not detected | Not detected |
Double-stranded exonuclease | Not detected | Not detected |
Double-stranded endonuclease | Not detected | Not detected |