PCR/qPCR/dPCR
PCR sensible, específica, robusta y fiable
El éxito de los experimentos de amplificación génica depende de varios factores críticos, como el rendimiento, la sensibilidad del ensayo, la robustez de la reacción incluso con moldes difíciles, y la normalización fiable de los datos. También puede ser necesario superar los retos específicos asociados al método de PCR que se utilice.
Elija el método que elija, ofrecemos numerosas soluciones optimizadas para PCR convencional, qPCR y dPCR. Nuestros kits, ensayos, instrumentos y accesorios le ayudarán a amplificar y detectar dianas con confianza y a optimizar el flujo de trabajo de prácticamente cualquier aplicación.
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
Examinar PCR/qPCR/dPCR
Productos
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DNA methylation analysis
157 productos encontrados
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
QIAGEN OneStep RT-PCR Kit
For highly sensitive and specific one-step RT-PCR
QIAGEN Multiplex PCR Kit
For highly specific and sensitive multiplex PCR without optimization requirements
Rotor-Gene Q (CA)
For outstanding performance in real-time PCR
QuantiTect Reverse Transcription Kit
For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis
HotStarTaq DNA Polymerase
For highly specific amplification with minimal optimization
RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis using laboratory-verified assays
REPLI-g Single Cell Kit
For highly uniform whole genome amplification (WGA) from single cells or limited sample material
QuantiTect RT-PCR Kits
Para la qRT-PCR de un paso mediante SYBR® Green I y qRT-PCR de un paso y qRT-PCR múltiple mediante sondas específicas de secuencia para el análisis de la expresión génica
RT2 SYBR® Green qPCR Mastermixes
For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
HotStarTaq Master Mix Kit
For highly specific amplification for any PCR application
QuantiTect PCR Kits
Para la qPCR y la qRT-PCR de dos pasos mediante SYBR Green I y para la qPCR y la qRT-PCR de dos pasos, así como la qPCR múltiple y la qRT-PCR de dos pasos y múlt. con sondas específicas de secuencia.
Rotor-Gene Q
For outstanding performance in real-time PCR
QuantiFast Pathogen +IC Kits
Para la detección sensible y fiable de ARN/ADN vírico y ADN bacteriano, incluido el control interno
HotStarTaq Plus DNA Polymerase
For fast and highly specific amplification in all applications
miRCURY LNA SYBR® Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Taq DNA Polymerase
Taq DNA Polymerase delivers fast PCR set up with minimal optimization for efficient amplification of routine, low-copy, longer or difficult targets
QuantiTect Primer Assays
For SYBR® Green-based expression analysis of individual genes using bioinformatically-verified assays
miRCURY LNA miRNA PCR Assays
Para una cuantificación sumamente sensible y específica de miARN con PCR de miARN optimizada con LNA y basada en SYBR® Green
miRCURY PCR Controls
Validated primer sets for normalization of miRNA expression levels in real-time PCR analyses
RT2 First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays
miRCURY LNA RT Kit
Para una síntesis óptima de ADNc de primera hebra con los miRCURY LNA miRNA PCR Systems utilizando SYBR Green o sonda de hidrólisis
HotStarTaq Plus Master Mix Kit
For fast and highly specific amplification in all applications
Custom Microbial DNA qPCR Arrays
For real-time PCR-based, customized microbial identification or profiling
QuantiTect Virus Kits
Para la detección muy sensible de ARN y/o ADN vírico
RT2 qPCR Primer Assays
For accurate and reliable gene expression analysis using laboratory-verified assays
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
QuantiNova PCR Kits
Para PCR y PCR múlt. en tiempo real bas. en sondas ultrarrápidas y de gran sensib. y espec. y para ob. de result. inmejorables con qPCR bas. en SYBR Green.
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
Omniscript RT Kit
For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
Custom RT2 Profiler PCR Arrays
For reliable and sensitive gene expression profiling using laboratory-verified assays
Microbial DNA qPCR Assays
For real-time PCR-based microbial detection; includes one assay with Microbial qPCR Mastermix for 100 reactions