Let’s talk custom
Need this product modified? Contact us to get started with tailored OEM solutions.
One-Step Probe RT-qPCR Mix ROX (100)
Cat. No. / ID: AM09.1-100
100 rxns (20 μL)
Mu-MLV Reverse Transcriptase and RNase Inhibitor and ROX solution are included in separate tubes.
Components: 2x 1 mL One-Step Probe RT-qPCR Mix, 40 μL 50x High ROX solution, 40 μL 50x Low ROX solution, 10 μL RNase Inhibitor, 10 μL Reverse Transcriptase, 1.9 mL Nuclease-free water
Storage temperature: Store all components at -20°C. It does not lose its activity after eight successive thaw cycles. Aliquoting can be applied if necessary.
OEM Product
One-Step Probe RT-qPCR Mix ROX
One-Step Probe RT-qPCR Mix
Reactions
100
500
The One-step Probe RT-qPCR Mixes is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Let’s talk custom
Need this product modified? Contact us to get started with tailored OEM solutions.
Features
- Detects low copies of RNA targets from a broad range of samples
- Exhibits consistent amplification across a wide dynamic range
- Eliminates non-specific amplification through precisely selected anti-Taq antibody
- Has a high efficiency in multiplex reactions
- Detects molecular targets accurately as fast as 40 minutes with two or three steps cycling profiles
Product Details
One-Step Probe RT-qPCR Mix is a convenient reaction mixture for fast and reliable quantitative Real-Time PCR using probes, including TaqMan ®, Scorpions ® and molecular beacon probes.
It was created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. It is the best choice for probe-based Real-Time PCR assays, including singleplex and multiplex gene expression studies.
Performance
| Assay | Specification |
|---|---|
| DNase contamination | None detected |
| RNase contamination | None detected |
Principle
Using high-affinity antibodies for hot-start polymerase ensures higher specificity by reducing the formation of primer-dimer structures. It allows for a more comprehensive dynamic range by removing competition for reaction reagents. It also leads to higher sensitivity and reproducibility.
Precisely optimized buffer components ensure optimal reverse transcriptase and hot-start polymerase activity conditions. Additionally, RNase Inhibitor protects RNA from unspecific RNases.
The One-Step Probe RT-qPCR Mix provides fast, highly specific one-step Real-Time RT-PCR results, giving consistent results across all commonly used Real-Time PCR platforms.
Procedure
Quality Control
One-Step Probe RT-qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of DNase and RNase contamination.
Applications
This is used for applications such as:
- RT-qPCR
- Gene expression analysis and genetic profiling
- miRNA profiling and quantification
- Mass screening and RNA viral pathogen detection
- Characterization of genetically modified organisms (GMO)
Resources
Certificates of Analysis (1)