PCR/qPCR/dPCR
๋ฏฟ๊ณ ์ฌ์ฉํ ์ ์๋ ํน์ด์ , ๋ฏผ๊ฐํ๋ฉฐ ๊ฒฌ๊ณ ํ PCR.
์ ์ ์ ์ฆํญ์ ์ํํ ๋ ์คํ์ ์ฑ๊ณต ์ฌ๋ถ๋ ์ฒ๋ฆฌ๋, ๋ถ์ ๋ฏผ๊ฐ๋, ๊น๋ค๋ก์ด ํ ํ๋ฆฟ์์๋ ๊ฒฌ๊ณ ํ ๋ฐ์, ์ ๋ขฐํ ์ ์๋ ๋ฐ์ดํฐ ์ ๊ทํ ๋ฑ ๋ช ๊ฐ์ง ์ค์ํ ์์์ ๋ฐ๋ผ ๋ฌ๋ผ์ง ์ ์์ต๋๋ค. ๋ํ ์ฌ์ฉ ์ค์ธ PCR ๋ฐฉ์๊ณผ ๊ด๋ จ๋ ํน์ ๋ฌธ์ ๋ฅผ ๊ทน๋ณตํด์ผ ํ ์๋ ์์ต๋๋ค.
์ด๋ค ๋ฐฉ์์ ์ ํํ์๋ , ๋น์ฌ๋ end-point PCR, qPCR, dPCR์ ์ต์ ํ๋ ๋ค์ํ ์๋ฃจ์ ์ ์ ๊ณตํฉ๋๋ค. ๋น์ฌ์ ํคํธ, ๋ถ์, ์ฅ๋น, ๋ถ์ํ์ ์์ ์๊ฒ ํ์ ์ ์ฆํญ ๋ฐ ๊ฒ์ถํ๊ณ ๊ฑฐ์ ๋ชจ๋ ์์ฉ ๋ถ์ผ์์ ์ํฌํ๋ก์ฐ๋ฅผ ๊ฐ์ํํ ์ ์๋๋ก ๋์์ค๋๋ค.
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ฮฮCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/ยตL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probeโbased or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
๊ฒ์ PCR/qPCR/dPCR
์ ํ
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DNA methylation analysis
156 ๊ฐ์ ์ฐพ์ ์ ํ
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
dPCR RNA-Plex Assays
For accurate and reliable probe-based gene expression analysis of mRNA and lncRNA targets in multiplex dPCR workflows.
QIAGEN OneStep RT-PCR Kit
One-step RT-PCR์ ๋น ๋ฅด๊ณ ์ฑ๊ณต์ ์ผ๋ก ํ ์ ์์ต๋๋ค
QIAGEN Multiplex PCR Kit
์กฐ๊ฑด์ ์ก์ ํ์์์ด ์ฝ๊ณ ๋น ๋ฅด๊ฒ multiplex ๋ถ์์ ์ํ ํ ์ ์์ต๋๋ค
Rotor-Gene Q (CA)
For outstanding performance in real-time PCR
QuantiTect Reverse Transcription Kit
Sensitive real-time PCR์ two-step RT-PCR๋ก ํ ๋ cDNA ํฉ์ฑ์ ์ ์ํ ํ ์ ์์ต๋๋ค
HotStarTaq DNA Polymerase
๋ชจ๋ applications์ ์ต์ ์ amplication์ด ๊ฐ๋ฅํฉ๋๋ค
RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis using laboratory-verified assays
REPLI-g Single Cell Kit
For highly uniform whole genome amplification (WGA) from single cells or limited sample material
QuantiTect RT-PCR Kits
์ ์ ์ ๋ฐํ ๋ถ์์ ์ํด SYBRยฎ Green I์ ์ฌ์ฉํ ์์คํ
qRT-PCR ๋ฐ ์์ด ํน์ด์ ํ๋ก๋ธ๋ฅผ ์ฌ์ฉํ ๋ฉํฐํ๋ ์ค qRT-PCR ๋ฐ ์์คํ
qRT-PCR์ ์ฌ์ฉ
RT2 SYBRยฎ Green qPCR Mastermixes
For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
HotStarTaq Master Mix Kit
High PCR specificity๋ฅผ ์ํ Premixed solution
QuantiTect PCR Kits
SYBR Green I์ ์ฌ์ฉํ qPCR ๋ฐ ํฌ์คํ
qRT-PCR์ฉ ๋ฐ qPCR ๋ฐ ํฌ์คํ
qRT-PCR์ฉ, ๋ํ ์์ด ํน์ด์ ํ๋ก๋ธ๋ฅผ ์ฌ์ฉํ ๋ฉํฐํ๋ ์ค qPCR ๋ฐ ํฌ์คํ
๋ฉํฐํ๋ ์ค qRT-PCR์ฉ
Rotor-Gene Q
For outstanding performance in real-time PCR
QuantiFast Pathogen +IC Kits
๋ฐ์ด๋ฌ์ค RNA/DNA์ ๋ฐํ
๋ฆฌ์ DNA์ ์์ ์ ์ธ ๊ณ ๊ฐ๋ ๊ฒ์ถ์ฉ, ๋ด๋ถ ๋์กฐ๋ฌผ์ง ํฌํจ
HotStarTaq Plus DNA Polymerase
๋ณ๋๋ก ์กฐ๊ฑด์ setupํ ํ์์์ด ํน์ด์ฑ์ด ๋์ hot-start PCR์ ์ํํ ์ ์์ต๋๋ค
miRCURY LNA SYBRยฎ Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Taq DNA Polymerase
For standard and specialized PCR applications
QuantiTect Primer Assays
For use in 2-step and 1-step qRT-PCR with SYBRยฎ Green detection for gene expression analysis
miRCURY LNA miRNA PCR Assays
LNA์ ์ต์ ํ๋ SYBRยฎ Green ๊ธฐ๋ฐ miRNA PCR์ ์ฌ์ฉํ๋ ๋งค์ฐ ๋ฏผ๊ฐํ๊ณ ํน์ด์ ์ธ miRNA ์ ๋ํ์ฉ
miRCURY PCR Controls
Validated primer sets for normalization of miRNA expression levels in real-time PCR analyses
RT2 First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays
miRCURY LNA RT Kit
SYBR Green ๋๋ ๊ฐ์๋ถํด ํ๋ก๋ธ๋ฅผ ์ฌ์ฉํ์ฌ miRCURY LNA miRNA PCR Systems์์ ์ต์ ์ ์ต์ด ๊ฐ๋ฅ cDNA ํฉ์ฑ
HotStarTaq Plus Master Mix Kit
Fast & specific amplification์ ์ํ premixed solution
Custom Microbial DNA qPCR Arrays
For real-time PCR-based, customized microbial identification or profiling
QuantiTect Virus Kits
๋ฐ์ด๋ฌ์ค RNA ๋ฐ/๋๋ DNA์ ๊ณ ๊ฐ๋ ๊ฒ์ถ์ฉ
RT2 qPCR Primer Assays
For accurate and reliable gene expression analysis using laboratory-verified assays
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
QuantiNova PCR Kits
๋์ ๋ฏผ๊ฐ๋, ํน์ด๋, ์ด๊ณ ์ ํ๋ก๋ธ ๊ธฐ๋ฐ real-time PCR ๋ฐ ๋ฉํฐํ๋ ์ค PCR์ฉ, SYBR Green ๊ธฐ๋ฐ qPCR์ ์ฌ์ฉํ ํ์ํ ๊ฒฐ๊ณผ ์ ๊ณต
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
Omniscript RT Kit
Reaction๋น 50 ng๋ถํฐ 2 ug๊น์ง์ RNA๋ฅผ reverse transcription์ ํ ์ ์์ต๋๋ค
Custom RT2 Profiler PCR Arrays
For reliable and sensitive gene expression profiling using laboratory-verified assays
Microbial DNA qPCR Assays
For real-time PCR-based microbial detection; includes one assay with Microbial qPCR Mastermix for 100 reactions
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
QuantiNova Reverse Transcription Kit
For fast cDNA synthesis and reproducible real-time two-step RT-PCR
QuantiNova Pathogen +IC Kit
๋ฐ์ด๋ฌ์ค RNA/DNA์ ๋ฐํ
๋ฆฌ์ DNA์ ์ด๊ณ ์ ๋์ ๊ฒ์ถ์ฉ, ๋ด๋ถ ๋์กฐ๋ฌผ์ง ํฌํจ
miRCURY LNA miRNA Custom PCR Panels
LNA ๊ฐํ SYBRยฎ Green ๊ธฐ๋ฐ miRNA qPCR ๋ถ์์ ๋ง์ถคํ 96-well ๋ฐ 384-well ํ๋ ์ดํธ
Taq PCR Master Mix Kit
ํธ๋ฆฌํ PCR setup์ ์ํ premixed solution
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QuantiNova RT-PCR Kits
์์ด ํน์ด์ ํ๋ก๋ธ๋ฅผ ์ฌ์ฉํ ๊ณ ๊ฐ๋ ๋ฐ ํน์ด์ ์์คํ
๋ฐ ๋ฉํฐํ๋ ์ค qRT-PCR ๋ฐ ๋ฉํฐํ๋ ์ค qRT-PCR ๋๋ ์ ์ ์ ๋ฐํ ๋ถ์์ ์ํ SYBRยฎ Green I์ ์ฌ์ฉํ ์์คํ
qPCR์ ์ฌ์ฉ
QuantiFast SYBRยฎ Green RT-PCR Kit
SYBR Green based real-time์ one-step RT-PCR๋ก ์ ์ํ ์ํํ ์ ์์ต๋๋ค
Taq PCR Core Kit
For standard and specialized PCR applications, includes dNTP mix
miRCURY LNA miRNA Focus PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBRยฎ Green-based miRNA qPCR
FastLane Cell RT-PCR Kits
For SYBRยฎ Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
REPLI-g Advanced DNA Single Cell Kit
For whole genome amplification (WGA) from single eukaryotic cells, limited samples or purified genomic DNA
miRCURY LNA miRNA miRNome PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBRยฎ Green-based miRNA qPCR
QIAGEN Fast Cycling PCR Kit
๋ชจ๋ thermal cycler๋ฅผ ์ด์ฉํ์ฌ ๋น ๋ฅด๊ณ ํน์ด์ฑ์ด ๋์ PCR์ ์ํํ ์ ์์ต๋๋ค
Sensiscript RT Kit
์๋์ RNA (์ฆ, carrier RNA๋ฅผ ํฌํจํ์ฌ 51 ng RNA ๋ณด๋ค ์ ์ ์)๋ก reverse transcription์ด ๊ฐ๋ฅํฉ๋๋ค
REPLI-g WTA Single Cell Kit
For whole transcriptome amplification of total RNA or mRNA from single cells
miScript miRNA Mimics
ํฉ์ฑ miRNA๋ฅผ ์ฌ์ฉํ miRNA ๊ธฐ๋ฅ ๋ฐ ์ ์ ์ ์กฐ์ ์ ๋ํ ์ฐ๊ตฌ
Microbial DNA qPCR Arrays
For real-time PCR-based, application-specific microbial identification or profiling
qBiomarker Somatic Mutation PCR Arrays
For rapid and accurate profiling of the somatic DNA mutation status of gene panels
QIAGEN OneStep Ahead RT-PCR Kit
For faster one-step RT-PCR with high sensitivity, specificity and fidelity
QuantiTect Whole Transcriptome Kit
For unlimited real-time PCR analysis from precious RNA samples
qBiomarker Somatic Mutation PCR Assays
For detecting the presence of specific DNA sequence mutations in cancer and oncogenesis
REPLI-g Cell WGA & WTA Kit
For parallel whole genome and whole transcriptome amplification from cells and limited samples
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
Microbial DNA qPCR Assay Kits
For real-time PCR-based microbial detection; includes one assay and everything required for a successful qPCR run for 20 reactions
miRCURY LNA miRNA Custom PCR Assays
For SYBRยฎ Green qPCR-based miRNA quantification using LNA-optimized miRNA PCR primer sets
miRCURY LNA miRNA PCR Starter Kit
SYBRยฎ Green์ ๊ธฐ๋ฐ์ผ๋ก ํ qPCR์ ์ฌ์ฉํ์ฌ miRNA ์ ๋ํ ์คํ์ ์์ํ๋ ๋ฐ ์ฌ์ฉ
RT2 SYBRยฎ Green FAST Mastermixes
For use with 100-ring disc format RT2 Profiler and RT2 lncRNA PCR Arrays on the Rotor-Gene Q
RT2 PreAMP cDNA Synthesis Kit
For preamplification of cDNA from small or FFPE samples of RNA for analysis on RT2 Profiler PCR Arrays
FastLane Cell cDNA Kit
RNA ๋ถ๋ฆฌ ์ ์ ๋จ๊ณ์์ด real-time PCR์ฉ cDNA๋ฅผ ํฉ์ฑํ ์ ์์ต๋๋ค
UCP Multiplex PCR Kit
Ultra-Clean Production master mix for multiplex hot-start PCR and microbiome applications
REPLI-g FFPE Kit
Formalin-fixed, paraffin-embedded (FFPE) tissue๋ก ๋ถํฐ ์ป์ด์ง DNA๋ฅผ ์ด์ฉํ์ฌ ๊ณง๋ฐ๋ก whole genome amplificationํ ์ ์์ต๋๋ค
qBiomarker Copy Number PCR Assays
For locus-specific analysis of copy number variations and alterations
RT2 lncRNA PCR Arrays
For lncRNA expression analysis by pathway and disease using laboratory-verified SYBRยฎ Green qPCR assays
REPLI-g Mitochondrial DNA Kit
Total DNA์ํ์ ํฌํจ๋ human mitochondrial DNA๋ก ๋ถํฐ ์ ๋ขฐ์ฑ์๋ whole genome amplification์ด ๊ฐ๋ฅํฉ๋๋ค
QIAGEN PCR Cloning Kits
For direct cloning of PCR products generated by Taq DNA polymerases
RNA Spike-In Kit, For RT
For quality control of the RNA isolation and cDNA synthesis steps of miRCURY LNA miRNA PCR experiments
Type-it Mutation Detect PCR Kit
For accurate and reliable multiplex PCR analysis of mutations
Certal Residual DNA Detection Kits
๋ด๋ถ ๋์กฐ๋ฌผ์ง์ ํฌํจํ ์๋ฅ CHO ์์ฃผ ์ธํฌ DNA์ ๊ณ ๊ฐ๋ ํ๋ก๋ธ ๊ธฐ๋ฐ ๊ฒ์ถ์ ์ฌ์ฉ
RT2 HT First Strand Kit
For cDNA synthesis and genomic DNA elimination in 96 RNA samples for use with RT2 Profiler PCR Arrays
Custom qBiomarker Somatic Mutation PCR Arrays
For rapid and accurate profiling of somatic mutations using a custom gene panel
miRCURY LNA miRNA QC PCR Panel
SYBRยฎ Green ๊ธฐ๋ฐ qPCR ํ๋กํ์ผ๋ง ์ ์ miRNA ์ํ์ ์ ๋ ๊ด๋ฆฌ๋ฅผ ์ฝ๊ณ ๋น ๋ฅด๊ฒ ์ํ
RT2 lncRNA qPCR Assay
For long noncoding gene expression analysis by real-time PCR
XpressRef Universal Total RNA
For use as a positive control for gene expression detection methods
QIAsymphony mericon Bacteria Kit
For automated isolation of pathogen DNA from primary bacterial enrichment cultures
UCP HiFidelity PCR Kit
Ultra-Clean Production master mix for high-fidelity hot-start PCR and microbiome applications
RT2 Profiler PCR Array Modification
For gene expression profiling using a partially modified pathway or disease panel
RT2 PreAMP Pathway Primer Mixes
For amplification of cDNA templates prior to analysis on RTยฒ Profiler PCR Arrays
RT2 Easy First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays
Microbial DNA qPCR Multi-Assay Kits
For real-time PCR-based, application-specific microbial identification or profiling; includes 3โ6 assay tubes in one box for 20 reactions
Rotor-Gene SYBRยฎ Green PCR Demo Kit
For evaluation of Rotor-Gene Q performance
Microbial DNA Positive Control
For use as a positive control for Microbial DNA qPCR Assays
mericon Pathogen Detection Assay
For real-time PCR detection of pathogen DNA in food or animal feed
qBiomarker SYBRยฎ Green Mastermixes
For use with qBiomarker Copy Number PCR Assays and Arrays
Custom RT2 PreAMP Pathway Primer Mixes
For amplification of cDNA templates prior to analysis on Custom RTยฒ Profiler PCR Arrays
RNA QC PCR Arrays
For quality assessment of RNA samples before characterization using PCR arrays
RT2 qPCR Primer gDNA Controls
For detection of genomic DNA contamination in RNA and cDNA
Supplemental Microbial qPCR Mastermix (ROX)
For real-time PCR applications
RT2 lncRNA PreAMP Primer Mixes
For cDNA synthesis prior to real-time PCR
mericon MeatTracker Kit
For detection of animal DNA in food, animal feed or pharmaceutical samples using real-time PCR
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
Supplemental Microbial qPCR Mastermix (Fluorescein)
For real-time PCR applications
ํ์ฐ์คํคํ ์ ์ ์
์๋ ์ ์ ์ ๋ฐํ ํ๋กํ์ผ๋ง ์คํ์ ์ ๊ทํํ๋ ๋ฐ ์ฌ์ฉ
miRCURY LNA miRNA Custom Bulk Plate
Customer-configurable SYBRยฎ Green-based miRNA qPCR Assays in convenient plate format
miRCURY LNA miRNA Custom Probe PCR Assays
LNA ๊ฐํ ํ๋ก๋ธ ๊ธฐ๋ฐ qPCR์ ์ฌ์ฉํ์ฌ ๋งค์ฐ ๋ฏผ๊ฐํ๊ณ ์ ํํ mature miRNA ์ ๋ํ
miRCURY LNA miRNA Probe PCR Assays
LNA ๊ฐํ ํ๋ก๋ธ ๊ธฐ๋ฐ qPCR์ ์ฌ์ฉํ์ฌ ๋งค์ฐ ๋ฏผ๊ฐํ๊ณ ์ ํํ mature miRNA ์ ๋ํ
miRCURY LNA Probe PCR Kits
miRCURY LNA miRNA Probe PCR System์ ์ฌ์ฉํ ๊ณ ์ฑ๋ฅ PCR์ฉ
qBiomarker Copy Number PCR Arrays
For profiling copy number variations and alterations
QIAcuity Probe PCR Kit
QIAcuity ๋์งํธ PCR ๊ธฐ๊ธฐ์ ํจ๊ป ์ฌ์ฉ
QIAcuity UCP Probe PCR Kit
QIAcuity ๋์งํธ PCR ๊ธฐ๊ธฐ์ ํจ๊ป ์ฌ์ฉํ๊ธฐ ์ํ Ultra Clean Production ํคํธ
QIAcuity OneStep Advanced Probe Kit
QIAcuity ๋์งํธ PCR ๊ธฐ๊ธฐ์ ์์คํ
RT-PCR์ฉ