PCR

Real-time qPCR

SARS-CoV-2 epidemiology

Reliable genotyping of virus isolates is essential to understand the evolution, virulence and transmission of SARS-CoV-2 and emerging variants. Moreover, during the respiratory virus season, when there is a significant overlap between SARS-CoV-2, flu A/B and RSV A/B symptoms, it is critical to accurately discriminate between the co-circulating viruses by multiplex, real-time PCR. Discover a robust, same-day genotyping approach and a simple, cost-effective multiplexing strategy to match your research goals.

COVID
PCR

Gene expression analysis

Analyzing gene expression by quantifying the corresponding mRNA or lncRNA is critical to many areas such as cancer and disease research and biomarker identification. For quantification of known targets, reverse transcription qPCR (RT-qPCR) remains the gold standard method, providing fast analysis from just a few to many genes

Our gene expression products enable sensitive quantification with any type of RNA or transcript abundance to deliver unbiased gene expression profiles and get you to your insights faster.

Female scientists

miRNA quantification and profiling

MicroRNAs (miRNAs) have emerged as potential biomarkers for numerous diseases and biological processes, such as cancer, heart disease, nervous system function, hemostasis and DNA repair.

Quantitative PCR is the tool of choice for analyzing known miRNA targets and offers several benefits over NGS. qPCR is fast, simple and inexpensive for most labs to set up and provides extremely sensitive analyses from low amounts of input RNA. It can be easily scaled to various research needs, from screening and profiling, to validation of NGS studies and verification of results from functional studies.

Microbial analysis

Standard PCR kits and reagents can introduce tiny amounts of contaminating nucleic acids into your experiments, jeopardizing results and conclusions.

Designed with microbiome and sensitive microbial applications in mind (e.g., microbial ID, food surveillance, pathogen detection, QC or residual DNA testing, and microbial-host interactions), our new UltraClean Production (UCP) real-time PCR kits bring greater precision to your experiments and confidence to your results.

UCP

Detecting microbial contamination

Up to 70% of mammalian cell cultures used to publish work are unknowingly contaminated by bacteria, viruses, or parasites. One company has set the course to help researchers and scientists detect contamination and obtain valid results. Perfectus Biomed has built a microbiology lab from the ground up using QIAGEN PCR technology to ensure valid results, cell line detection, and no contamination.

dPCR
PCR

DNA quantitation

Quantitation of DNA targets plays an important role in many research areas: from detection of genetically modified organisms (GMO) in food to the analysis of genetic variations such as single nucleotide polymorphisms (SNPs), copy number variations (CNV) and somatic mutations.

Our selection of QuantiNova Real-Time PCR Kits help remove the experimental variation and human errors from your qPCR workflow to deliver precise and reproducible quantitation.

Analyzing copy number variation? Go digital.
Digital PCR can easily detect small fold-change differences such as less than a 1.2-fold change in CNV from four to five copies, without requiring a standard curve.
qPCR

Discover the benefits of QuantiNova 

QuantiNova kits represent today’s most advanced portfolio of real-time PCR and RT-PCR kits for gene expression analysis, gene quantification and pathogen detection. A unique combination of in-process controls removes variables and minimizes human error, so your experiments are bound to work. Simple and fast procedures increase your efficiency, saving you time and money. Plus, flexibility in detection chemistry and full real-time cycler compatibility mean there’s no need to compromise.

Experience the benefits of QuantiNova ​

Efficient and foolproof workflow
Efficient and foolproof workflow
The very simple and fast procedures just work, so you can finish your qPCR jobs sooner and more affordably – the first time. Plus, room temperature setup supports workflow automation for even more efficiency gains.
Precise results you can trust
Precise results you can trust
In process monitoring tools – including visual pipetting verification, internal control RNA and integrated gDNA removal – minimize variation and human error, giving you confidence in your results.
Flexibility and options
Flexibility and options
You can choose – fluorescent dye- or probe-based detection technology, one- or two-step RT-PCR, singleplex or multiplex, real-time cycler platform. Plus, you can easily connect to related NGS and dPCR assay technologies via GeneGlobe.

Looking for one of our other qPCR solutions?

Does your project have unique requirements? Our complete range of real-time PCR solutions has you covered. Check out these other offerings or contact one of our experts to find the best tools for your research.

QuantiTect

FastLane

Microbial DNA qPCR

Contact us

Ensuring high-quality, reproducible results

qPCR is a ubiquitous research tool, but improperly designed experiments will generate questionable findings. The MIQE guidelines define the standards for designing and reporting qPCR experiments.

What makes a good qPCR assay? High accuracy, dynamic range, reliability, reproducibility, sensitivity and specificity. The primer design algorithm and the reaction buffer conditions must work together to provide the best results. Designing assays without fully understanding the critical design aspects can unknowingly compromise the results.

We can help remove the guesswork from your experiments. Our broad selection of qPCR kits and an unparalleled choice of predesigned assays and panels along the option to design your own using our advanced algorithms ensure quality qPCR results time and again.

Consider these factors for useful and meaningful results

Experiment design
Experiment design
Define the problem, form hypotheses and cover all factors. Consider controls, type and number of replicates, sample handling and sources of variation.
Sample management
Sample management
mRNA profiles can quickly change after tissue/blood collection. Standardize sampling techniques and timepoints and use a reliable RNA stabilization method.
RNA isolation / gDNA removal
RNA isolation / gDNA removal
gDNA contamination leads to inaccurate quantification. Avoid unwanted DNA detection by removing gDNA or using specially designed primers.
Reference gene selection
Reference gene selection
Choose appropriate reference genes to avoid bias. Consider using 2–5 validated, stably-expressed reference genes for normalization.
Data analysis
Data analysis
Enable transparent data exchange by using real-time cyclers with built-in RDML export capabilities. Ensure accurate data processing and interpretation.
Not sure which PCR products to pick?
Not sure which PCR products to pick?
This smart online tool can help you find the right products and instruments for every step of your protocol.
Tty it now
PCR tips & tricks workshop
PCR tips & tricks workshop
Hear our panel of experts share how you can overcome the most common PCR pain points.
Watch now
Designing optimal assays
Designing optimal assays
Find out how to achieve optimal primer designs for successful RNA and miRNA qPCR profiling.
Watch webinar
Achieving reproducible results
Achieving reproducible results
Learn how to tackle qRT-PCR challenges and achieve high accuracy and reproducibility.
Watch webinar
GeneGlobe Design & Analysis Hub
GeneGlobe Design & Analysis Hub
One place to explore targets and pathways, find or customize PCR assays and panels and analyze your data.
Discover GeneGlobe

Guides and tips for great results​

10 tips to maximize your qPCR success​
10 tips to maximize your qPCR success​
Improve your real-time PCR by simply following these tips and tricks
Discover top tips
Choosing your qPCR detection chemistry​
Choosing your qPCR detection chemistry​
SYBR® Green vs. hydrolysis probe: each has its own unique benefits. Which is right for you?
Read about it
Biofluid miRNA profiling guidelines​
Biofluid miRNA profiling guidelines​
Maximize your miRNA qPCR success for biofluid and liquid biopsy samples
Get the guidelines
Avoid common pitfalls in real-time RT-PCR​
Avoid common pitfalls in real-time RT-PCR​
Find out more in our application note: Guidelines for real-time RT-PCR assay optimization
Download now

Raise your qPCR workflow to the next level

It is increasingly challenging to find the right real-time PCR cycler for your laboratory’s application requirements. For best results, you need low operating costs, high throughput, the ability to multiplex with fast analysis times and reliable reproducibility. As the list of features and specifications grows, an understanding of some of the key performance benefits such as precision, uniformity, dynamic range and resolution becomes critical.

Choose the instrument that suits your needs from our portfolio of real-time PCR cyclers.

QIAquant cyclers​
QIAquant cyclers​
All-round systems for fast and high performance in standard or high-throughput real-time PCR applications such as gene expression analysis, genotyping and pathogen detection
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Rotor-Gene Q​
Rotor-Gene Q​
Multiple optimized design features enable outstanding performance and reliable results in specialty applications such as FRET, HRM and 6+plex analysis
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Featured topics

Digital PCR
Digital PCR
Go beyond the limits of real-time PCR for even higher accuracy, sensitivity and absolute quantification
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Microbiome research​
Microbiome research​
Reliable and dedicated solutions that address the specific demands of microbial analysis workflows
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Liquid biopsy
Liquid biopsy
Discover, detect and validate biomarkers in circulating cell-free nucleic acids, CTCs and exosomes.
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Molecular biology resources
Molecular biology resources
Check out this portal to find numerous resources and tools that can help you navigate the twists and turns of your research
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