Pyrosequencing Applications

Quantitative data for new horizons in genetic research

Pyrosequencing is a unique detection technology based on the principle of sequencing-by-synthesis, which provides quantitative real-time data without the need for gels, probes, or labels. In addition to characterizing single nucleotide polymorphisms (SNPs), insertion-deletions (indels), and unknown sequence variants, it can sensitively quantify allele frequencies and DNA methylation levels at both CpG and non-CpG (CpN) sites. Integrating detection and quantification of genetic variation into one powerful system, Pyrosequencing with the PyroMark platform outperforms other sequence-based solutions in the analysis of targeted short DNA sequences. For a range of applications, this means reduced cost and time investment.

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DNA Methylation Analysis
DNA Methylation Analysis
Highly sensitive quantitative methylation analysis at consecutive CpG and non-CpG (CpN) sites
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Mutation Analysis and Genotyping
Mutation Analysis and Genotyping
Unambiguous, fully quantitative genotyping that distinguishes multi-site variations from single nucleotide polymorphisms
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Microbial ID and Drug Resistance Typing
Microbial ID and Drug Resistance Typing
Versatile and reliable microbial genotyping and quantitative analysis of sequence variations
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