Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted by relevance Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be? FAQ ID -2717 View What is the composition of Buffer S3? FAQ ID -2788 View What MOI should I use for my cells? FAQ ID -2770 View What RT² qPCR Primer Assays are available? FAQ ID -2708 View What transfection reagents are compatible with the Cignal Reporter Assays? FAQ ID -2764 View What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture? FAQ ID -2777 View What is included in the SureSilencing shRNA Plasmids shipment? FAQ ID -2785 View What is the composition of Buffer RLC? FAQ ID -2795 View What is the composition of Buffer RLT? FAQ ID -2793 View What is the composition of Buffer RLT plus? FAQ ID -2794 View What is the composition of Buffer BB? FAQ ID -2790 View What is the composition of Buffer ETR? FAQ ID -2789 View What is the composition of Buffer PB? FAQ ID -2791 View What are the key parameters contributing to unwanted off-target effects in an RNAi experiment? FAQ ID -2775 View What are the structures of the siRNA molecules used in RNAi studies? FAQ ID -2760 View What are the critical factors in designing the siRNA molecules to be used for RNAi studies? FAQ ID -2759 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View What is the composition of gDNA Eliminator Solution? FAQ ID -2799 View What is the composition of Buffer RW1? FAQ ID -2796 View What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays? FAQ ID -2739 View What is the composition of Buffer RWT? FAQ ID -2798 View Can miScript miRNA qPCR Assays be used for pre-miRNA detection? FAQ ID -2724 View Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs? FAQ ID -2723 View Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array? FAQ ID -2736 View Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays? FAQ ID -2725 View Do you always run samples in triplicates? FAQ ID -2703 View Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit? FAQ ID -2722 View Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids? FAQ ID -2787 View Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides? FAQ ID -2737 View Can I make stable cell lines with the Cignal Reporter Assays? FAQ ID -2763 View Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector? FAQ ID -2783 View How are the primers in the EpiTect Methyl qPCR Array designed? FAQ ID -2735 View On which instrumentation will the RT² Profiler PCR Array work? FAQ ID -2719 View Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays? FAQ ID -2747 View What are the advantages of using the SureSilencing shRNA Plasmids over siRNA? FAQ ID -2786 View The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions? FAQ ID -2750 View The pathway reporter luciferase activity values are greater than the positive control, is there a problem? FAQ ID -2766 View The pathway reporter luciferase activity values are less than the negative control, is there a problem? FAQ ID -2767 View How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 View How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 View How can RNA interference be used as a life science research tool? FAQ ID -2756 View How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 View How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 View How can I normalize my EpiTect ChIP results? FAQ ID -2751 View What is the recommended amount of input template for each RT² qPCR Primer Assay? FAQ ID -2716 View What is the RT² Profiler PCR Array? FAQ ID -2718 View Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 View Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 View Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 View Can I manually set the threshold line? FAQ ID -2702 View Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 View Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 View Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 View Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 View Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 View At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 View Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 View Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 View Can I transform the Cignal Reporter Assays? FAQ ID -2762 View Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 View How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 View How many housekeeping genes are included in each PCR Array? FAQ ID -2704 View Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 View What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 View How reliable are the results from the miScript Primer Assays? FAQ ID -2730 View What are EpiTect ChIP qPCR Assays? FAQ ID -2720 View How do you determine the efficiency using the PCR array? FAQ ID -2701 View How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 View How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 View How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 View How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 View How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 View What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 View Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 View What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 View 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? View 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? View 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? View 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? View 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? View 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? View 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? View 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? View What sample types can be tested on the arrays/assays? FAQ ID — 3399 View What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 View Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 View What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 View View What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 View What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 View 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 View 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 View 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 View 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 View 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 View 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 View 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 View Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 View 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? View 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? View 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? View 3314-What is the concentration of RNase A sold separately? View CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 View 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? View Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 View Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 View When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 View Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 View Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 View What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 View Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 View Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately? FAQ ID - 3389 View What is the composition of elution buffers used in QIAsymphony DNA Investigator kits? FAQ ID - 3387 View Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately? FAQ ID - 3390 View 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 View View 3340 - Which operating system is compatible with PyroMark IdentiFire Software? View 3325 - What are the catalog numbers for the packing materials for the QIAcube? View 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? View 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? View 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? View 3337 - Which method is used for QIAGEN sequencing service? View 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? View 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? View 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 View 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 View 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? View 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? View 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? View 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? View 3320 - What is the sensitivity of the Type-it HRM PCR Kit? View 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? View 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? View Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388 View Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 View 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? View View View View 3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit? View 3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit? View 3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit? View 3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit? View 3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit? View What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit? FAQ ID -499 View What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap? FAQ ID - 3364 View 3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits? View 3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? View I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do? FAQ ID - 3363 View 3302 - How long can I store my purified RNA? View Do you sell CoralLoad dye separately? FAQ ID - 3368 View 3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit? View 3194 - What DNA fragment sizes are required for the Investigator DIPlex kit? View 3195 - Where can I find the Population Data for the Investigator DIPlex kit? View 3196 - What is the DIPsorter Freeware? View 3188 - How many His residues are required for successful purification on Ni-NTA resins? View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care