Product FAQ

 

You are not authorized to download the resource

Sorted alphabetically
Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
Will REPLI-g work at high temperatures?
FAQ ID -656
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
Will QIAseq FastSelect work in other species?
FAQ ID -147911
Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
Will pipetting error affect the miRNA qPCR Assay results?
FAQ ID -2728
Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741
Will pancreas tissue swim on top of RNAprotect?
FAQ ID -536
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2991
Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
Why should I work with diluted or undiluted screens?
FAQ ID -2419
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
Why is there no male DNA obtained from sexual assault samples?
FAQ ID - 143767
Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
Why is the copy number call lower than expected?
FAQ ID — 3413
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
Why is my plasmid DNA yield low?
FAQ ID -768
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
Why is it not recommended to stabilize cells with RNAprotect?
FAQ ID -941
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
Why is DTT used only for semen samples?
FAQ ID - 143761
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
Back to top