Unravel the complexity of the genome with DNA-seq solutions

Redefine amplicon sequencing: Find the variant that matters with targeted DNA-seq

PCR duplicates, false positives and library bias can undermine the quality of your targeted sequencing data. Introduce precision and confidence into your workflow by adopting the digital DNA sequencing approach.

Our platform-agnostic, expertly curated, targeted DNA sequencing panels utilize unique molecular indices to ensure superior coverage of:

• GC-rich genomic regions
• Exonic regions of genes
• Hotspots
• SNPs
• Intronic and promoter regions

Error or low-frequency mutation?

Targeted DNA sequencing with panels is a powerful approach to detect low-frequency variants. However, a major drawback is that all DNA fragments look exactly the same, making it almost impossible to distinguish sequencing errors and unique DNA molecules. This limits your ability to detect low-frequency DNA variants confidently. Not with QIAseq – all catalog and custom panels contain unique molecular indices, which tag the original DNA molecules and eliminate false reads due to PCR, polymerase and machine-read errors.

Access the most difficult regions of the genome

QIAseq products include optimized amplicon sequencing buffers and enzymology, allowing you to access the most difficult regions of the genome. The chemistry used in the QIAseq Targeted DNA panels and workflow is compatible with both regular and GC-rich genomic regions. Achieve 100% coverage for genes rich in GC content such as CEBPA and CCND1 using tiling methods.

Automatable and scalable exome sequencing with a single-day workflow

Examining the exome to identify disease-causing variants but worried about coverage uniformity due to GC composition?

Detect pathogenic variants with confidence, ease and efficiency with our scalable single-day, automation-compatible workflow. Based on hybrid capture technology, QIAseq Human Exome Kits ensure:

• Highly sensitive variant calling of targets of >500 genes
• Reduced sequencing costs per sample by 50%
• A 40% decrease in overall turnaround time from sample to data

Seamless integration with QIAGEN CLC Genomics Workbench enables rapid variant calling and QCI Translational ensures accurate variant interpretation and disease-specific insights – without the burden of manual curation.

Capture a comprehensive view of the genome

Whole genome and hybrid capture sequencing provide a holistic view of the entire genome and unparalleled insights, but they can be time-and cost-intensive approaches. QIAseq solutions make these methodologies more accessible by maximizing per-sample data quality.

Benefit from:

• Advanced chemistries for picogram sample input and fast, automatable protocols
• Enzymatic DNA fragmentation – no need for expensive mechanical shearing equipment
• Increased ligation efficiencies for high conversion rates and yields – no adapter-dimers
• Uniform G/C representation from any organism
• Convenient kit formats with included dual-barcoded adapters

Featured success story

Dr. Egbert Schulze from the Endocrinology and Nuclear Medicine Community Practice, Heidelberg, discusses how the all-enzymatic QIAseq FX workflow enabled uniform coverage and helped him achieve reliable sequencing results in his research.