GeneRead Size Selection Kit

快速、可靠的去除小于150 bp的DNA片段，用于NGS应用的文库制备

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GeneRead Size Selection Kit (50)

Cat. No. / ID: 180514

For 50 reactions: Spin columns and buffers

Inquire

GeneRead Size Selection Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

准确的筛选DNA片段
基于QIAGEN成熟的硅胶模纯化柱技术，实验快速
实验操作便利，可在QIAcube自动化核酸纯化仪上自动进行实验

产品详情

GeneRead Size Selection Kit采用便利的纯化柱技术，准确的筛选DNA片段并进行纯化。该试剂盒含有MinElute纯化柱和优化的结合缓冲液，用于选择长度大于150 bp的DNA片段。

绩效

快速、准确的选择纯化DNA片段
GeneRead Size Selection Kit采用便利的纯化柱技术，确保准确选择纯化DNA文库，同时高效去除接头二聚体或接头单体（参见）。短于150 bp的DNA片段被可靠去除。

查看图表

Precise size selection.

原理

GeneRead Size Selection Kit结合了便利的纯化柱技术和独特设计的结合缓冲液的选择性结合特性。该试剂盒中的MinElute纯化柱能够纯化获得高浓度DNA，用于后续反应。该试剂盒中的特殊缓冲液可高效去除小DNA片段，如接头和接头二聚体。在优化浓度的盐溶液中，较大的DNA片段可吸附在硅胶模上，其他小片段DNA和污染物流出纯化柱。这一选择性吸附的分界线（电泳分析可视条带的最小片段的长度）为150 bp。先前实验残留的酶等其他杂质被高效洗去，洗脱获得纯化的特定长度的DNA。再进行两次纯化，去除接头单体和二聚体，并获得较高的DNA回收率，用于选择特定长度的DNA片段，制备文库，用于后续二代测序应用。

程序

GeneRead Size Selection Kit采用便利的纯化柱技术，准确选择性纯化特定大小的DNA，高效去除接头二聚体和接头单体。该试剂盒可在QIAcube全自动核酸纯化仪上自动实验，便利的实验方案无需繁琐的手动操作，极大节省时间，并且确保纯化产物无乙醇或纯化珠残留。

应用

GeneRead Size Selection Kit能够快速、可靠的去除小于150 bp的DNA片段，用于NGS文库制备。

辅助数据和图表

Precise size selection.

The GeneRead Size Selection Kit effectively removes adapter dimers and adapter monomers following library preparation. A scaled-up image of the above data showing the correct size distribution of Illumina-compatible library fragments following size selection is shown in inset. FU: Fluorescence units.

资源

Protocol Sheet for QIAcube Classic - GeneRead Size Selection - sheared DNA protocol

Accelerate your NGS performance through Sample to Insight solutions

For the preparation, size selection, and purification of DNA libraries for NGS applications

Cleanup of sheared DNA and size selection

This protocol is for cleanup and reliable removal of DNA fragments <150 bp for library preparation in NGS applications.

Sample Size	140 µL
Elution volume	17 µL
Applications	Cleanup
Starting material	DNA

For fast and reliable removal of DNA fragments <150 bp for library preparation in next-generation sequencing (NGS)

Download Safety Data Sheets for QIAGEN product components.

FAQ

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560

Full recovery is seen for fragments larger than 300bp. Between 150 and 300bp, recovery increases with size.

It is normal for the buffer to turn yellow and it does not impact the performance of the buffer.

FAQ ID - 3562