GeneRead QIAcube

For automating and streamlining library template preparation

Products

The GeneRead QIAcube is for Research Use Only. Not for use in diagnostic procedures. Please note: The GeneReader NGS System is currently only available with proprietary new sequencing chemistry in the US. Legacy sequencing chemistry is only available ex-US.

GeneRead QIAcube (230 V)

Cat. No. / ID: 9002345

Robotic workstation for automation of clonal amplification: includes installation and training, 1 year warranty on parts and labor

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GeneRead QIAcube (110 V)

Cat. No. / ID: 9002344

Robotic workstation for automation of clonal amplification: includes installation and training, 1 year warranty on parts and labor

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GR QIAcube, Premium Agreement

Cat. No. / ID: 9243848

On-site GeneRead QIAcube instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.

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GR QIAcube, Full Agreement

Cat. No. / ID: 9243846

On-site GeneRead QIAcube instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.

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GR QIAcube, Preventive Subscription

Cat. No. / ID: 9243850A

One on-site Preventive Maintenance or Inspection Service visit for the GeneRead QIAcube, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

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Features

Significant reduction of hands-on time and increased reproducibility
Scalable preparation of between 1 and 4 library sequencing template pools in parallel
Intuitive touchscreen guiding you through set-up
Built on the success of the QIAcube platform
Proven performance with the GeneReader NGS System

Product Details

The GeneRead QIAcube provides affordable automation for the clonal amplification step of next-generation sequencing template preparation. With a small footprint and intuitive user interface, the GeneRead QIAcube reduces hands-on time to less than 40 minutes and enables simultaneous preparation of multiple library pools.

Principle

The GeneRead QIAcube is designed to automate clonal amplification using the GeneRead Clonal Amp Q Kit. The GeneRead QIAcube controls integrated components, including a centrifuge, heated shaker, magnetic station, pipetting system and robotic gripper. The GeneRead QIAcube has been validated with the GeneReader NGS System.

Procedure

The GeneRead QIAcube instrument is operated via the integrated and intuitive GUI which allows you to select and run protocols and save data files. The user is guided step by step through the workdeck set-up and running of protocols. No additional user documentation is required.When the GeneRead QIAcube is running, the touchscreen displays information on protocol status and the remaining run time. The instrument allows for preparation of 4 library pools per run. Manual interactions are reduced to 40 min for template preparation of 4 library pools. By multiplexing the output capacity can be increased to up to 40 samples per run using 4 x 10 plex.

Applications

The GeneRead QIAcube is designed to automate the relevant steps of the template preparation for the GeneReader NGS System platform.

Resources

Download Safety Data Sheets for QIAGEN product components.

FAQ

For an up-to-date list of available protocols, visit QIAcube Standard Protocols.

Click here for a virtual demonstration on how the QIAcube operates.

FAQ ID -1403

Yes. The heated shaker on the QIAcube can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the shaker, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.7 'Operating the shaker'.

The shaker can be used to heat and shake samples simultaneously, shake samples without heating, or heat samples without shaking.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a virtual demonstration of the heater and shaker on the QIAcube.

FAQ ID -1422

Yes. The QIAcube centrifuge can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the centrifuge, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.8 'Operating the Centrifuge'.

FAQ ID -1423

Yes, this is absolutely necessary. If the lids are not securely fixed in the rotor adapter slots during processing on the QIAcube, they will become detached from the elution tubes and columns during centrifugation and will collect at the bottom of the centrifuge chamber.

FAQ ID -1415

The following disinfectants and detergents are recommended for cleaning the QIAcube:

Mikrozid® Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g Mikrozid Liquid)
RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door
RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)
Note: Alcohol is not recommended for cleaning of the door.

Europe

Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15–30% nonionic surfactants).

USA

DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride).

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

FAQ ID -1416

No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a demonstration on how the QIAcube is used with the RNeasy Mini Kit for automated purification of RNA.

FAQ ID -1406

No, it is not possible to connect the QIAcube with laboratory computer networks.

FAQ ID -1411

The QIAcube has the following weight and dimensions:

Weight: 70.5 kg (155.4 lb.)
Width: 65 cm (25.6 in.)
Depth: 62 cm (24.4 in.)
Height (door closed): 57 cm (22.4 in.)
Height (door open): 81 cm (32 in.)

FAQ ID -1407

Please refer to section 6.5 'Decontaminating the QIAcube' in chapter 6 'Maintenance Procedures' of the QIAcube User Manual.

FAQ ID -1419

Yes, rotor and/or buckets can be removed from the QIAcube for cleaning. For information on how to remove these parts, refer to the QIAcube User Manual.

FAQ ID -1410

You can stop a QIAcube protocol if there is an emergency by pressing “Cancel”. To confirm that you want to stop the protocol run, press “OK”. To cancel the 'stop protocol' command, press “Cancel”.

Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.

FAQ ID -1414

The corresponding manual kits can be used for all available protocols on the QIAcube. The manual kits contain sufficient buffers and reagents to process the number of spin columns provided with the kit.

In addition to the purification kit, Rotor Adapters (10 x 24), cat. no 990394, and the required disposable tips:

Filter-Tips, 200 µl (1024), cat no. 990332,
Filter-Tips, 1000 µl (1024), cat no. 990352,
Filter-Tips, 1000 µl wide-bore (1024), cat no. 990452,

need to be ordered separately. To find out which filter-tips are required for a specific application, refer to the corresponding protocol sheet.

FAQ ID -1402

No. Autoclaving the rotor adapters will cause the plastic to deform and the rotor adapters will no longer fit properly into the centrifuge buckets.

FAQ ID -1409

No. The QIAcube is equipped with an optical sensor, which determines the tip size and the number of tips loaded onto the worktable prior to starting a run.

The QIAcube can use partially used tip racks from previous runs, provided that the racks contain sufficient tips to process the number of samples loaded for the current run.

FAQ ID -1418

Yes. At the end of each protocol run on the QIAcube, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.

FAQ ID -1412