ipsogen JAK2 MutaScreen Kits

采用real-time PCR对JAK2 V617F/G1849T突变进行半定量检测

Products

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ipsogen JAK2 MutaScreen Kit (10)

Cat. No. / ID:  673012

For 10 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
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ipsogen JAK2 MutaScreen Kit (24)

Cat. No. / ID:  673013

For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
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ipsogen JAK2 MutaScreen RS Kit (19)

Cat. No. / ID:  673113

For 19 reactions: V617F Positive Control, V617F Negative Control, V617F Reference Scale, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
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Features

  • 可靠的半定量检测
  • 多重分析,无需优化
  • 即用型解决方案,便利的工作流程

Product Details

ipsogen JAK2 MutaScreen Kits仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对基因组DNA中的JAK2 V617F/G1849T突变进行灵敏、可靠的半定量检测。

Performance

为确保检测的准确性,ipsogen NJAK2 MutaScreen Kit已经过优化,利用DNA阳性和阴性对照,检测JAK2 V617F/G1849T突变(参见 Representative allelic discrimination experiments for qualitative detection of the JAK2 V617F/G1849T mutation in genomic DNA Representative allelic discrimination experiments for semi-quantitative detection of the JAK2 V617F/G1849T mutation in genomic DNA)。ipsogen JAK2 MutaScreen Kits的样本阳性DNA浓度检测阈限为2%,推荐的起始样本DNA量最少为25 ng。
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Principle

ipsogen JAK2 MutaScreen Kits为即用型试剂盒,能够对基因组DNA中SNP导致的的JAK2 V617F/G1849T体细胞突变进行半定量检测分析(参见 JAK2 mutation site identification)。该试剂盒能够获得可靠、可重复性的结果。ipsogen JAK2 MutaScreen Kits提供JAK2 V617F的DNA检测临界样本(COS)、DNA阳性对照(PC)和阴性对照(NC)。使用检测临界样本和对照能够实现准确的基因分型分析。ipsogen JAK2 MutaScreen RS Kit提供V617F的参比。
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Procedure

第一步是抽提总DNA,并检测其量与品质。第二步是通过real-time PCR扩增DNA。ipsogen JAK2 MutaScreen Kits能够对JAK2 V617F/G1849T突变进行可靠的检测和半定量。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

Applications

ipsogen JAK2 MutaScreen Kits能够对JAK2 V617F/G1849T突变进行可靠的检测和半定量,仅适用于科研。

Supporting data and figures

Resources

试剂盒操作手册 (2)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.