ipsogen NPM1 mut A, B&D MutaQuant Kits

采用real-time PCR对NPM1基因转录本的A、B和D型突变进行定量检测

Products

ipsogen NPM1 mut B&D MutaQuant Kit (24)

Cat. No. / ID: 677613

For 24 reactions: ABL Control Gene Standards, NPM1 MutB Gene Standards, NPM1 MutD Gene Standards, Primer and Probe Mix ABL, Primer and Probe Mix NPM1 mutB Mutated Gene, Primer and Probe Mix NPM1 mutD Mutated Gene

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ipsogen NPM1 mut A MutaQuant Kit (24)

Cat. No. / ID: 677513

For 24 reactions: ABL Control Gene Standards, NPM1 MutA Gene Standards, Primer and Probe Mix ABL, Primer and Probe Mix NPM1 mutA Mutated Gene

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Features

灵敏的定量检测试剂
特异性定量检测A、B和D型突变
对与ABL对照基因表达相关的转录本进行准确定量
卓越的质量保证，获得ISO 13485认证
即用型解决方案，便利的工作流程

Product Details

ipsogen NPM1 mut A, B&D MutaQuant Kits仅适用于科研，不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。ipsogen NPM1 mut A, B&D MutaQuant Kit中的试剂经过优化，能够NPM1基因转录本的特定突变型（A、B和D）进行可靠、灵敏的定量检测。

Performance

为确保获得高检测灵敏度，ipsogen NPM1 mut A, B&D MutaQuant Kit经过优化，能够检测宽泛的线性范围内的NPM1基因转录本A型、B型和D型突变（参见Sensitive detection of ABL and NPM1 mut A, B, and D standards）。

Principle

ipsogen NPM1 mut A, B&D MutaQuant Kits为即用型试剂盒，能够定量检测人类RNA中NPM1基因转录本A型、B型和D型突变（参见NPM1 alleles）。检测结果为相对于ABL对照基因的相对定量结果（参见ABL control gene transcript）。该试剂盒灵敏度高，可重复性强。ipsogen NPM1 mut A, B&D MutaQuant Kit为ABL基因提供3个标准品稀释液，为NPM1基因提供5个标准品稀释液。

Procedure

第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增DNA。ipsogen NPM1 mut A, B&D MutaQuant Kits能够对NPM1基因转录本的特定突变型（A、B和D）进行可靠、灵敏的定量检测。只需按照试剂盒操作手册中的实验方案，即可便利的开始反应。

Applications

ipsogen NPM1 mut A, B&D MutaQuant Kits能够对NPM1基因转录本的特定突变型（A、B和D）进行可靠、灵敏的定量检测，仅适用于科研。

Supporting data and figures

Sensitive detection of NPM1 mut A and ABL standards.

Detection of [A] NPM1 mut A standards (M1 to M5) with 10¹, 10², 10³, 10⁵, and 10⁶ copies/5 μl and [B] ABL standards (C1, C2, C3) with 10³, 10⁴, and 10⁵ copies/5 μl.

Resources

Download Safety Data Sheets for QIAGEN product components.