Bacteria, fungi, viruses and parasitic metazoans are ubiquitous in the environment and are part of all aspects of human life, from human health to food production. For example, the human microbiome, found on and in our bodies, is estimated to include about ten times more microbes than cells in the human body. In turn, each microbe can trigger various harmful or beneficial effects on humans. This makes the specific detection and monitoring of microbes important for understanding their biological function, especially in infection or colonization of the human body.
In this webinar, we will introduce DNA-based methods for detecting and identifying microbial species and microbial genes, focusing on hydrolysis probe-based assays and digital PCR. We will present the new dPCR Microbial DNA Detection Assay portfolio that enables rapid profiling and identification of microbial species, antibiotic resistance genes and virulence genes from diverse samples. These may include wastewater samples, infectious diseases, human pathogens, the human microbiome, multiple drug resistance, sepsis, food production or environmental samples.

You will learn:

• How to set up a dPCR microbial DNA detection experiment
• What targets can be analyzed
• How to analyze multiple targets in one reaction
• How to handle viral RNA targets