In this presentation, Dr. Ina Scheuerpflug will share her expertise on the critical steps and factors you need to know for efficient and precise gene expression studies using real-time PCR technology. Ina will conclude with a discussion of the NEW QIAGEN solutions for real-time PCR, the QIAquant Instruments.

In this webinar, Ina will cover:

• Introduction to gene expression analysis;
• Critical steps for real-time PCR analysis;
• Improved methods for cDNA synthesis;
• Real-time PCR essentials:
  • PCR kinetic and CT/Cq = threshold cycle
  • Absolute and relative quantification methods
  • Endogenous reference genes
  • Factors influencing PCR specificity
  • Different amplicon detection methods
  • Fluorescent dyes – the principle
• NEW Real-Time PCR solutions from QIAGEN.