Product FAQ

 

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What is the expected amplicon size of the Microbial DNA qPCR Assays?
FAQ ID — 3396
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
What sample types can be tested on the arrays/assays?
FAQ ID — 3399
What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?
FAQ ID — 3397
Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
What is the QIAxcel QX RNA Size Marker 200-6000nt?

FAQ ID - 3365

What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?
FAQ ID - 3366
3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
3314-What is the concentration of RNase A sold separately?
CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
FAQ ID - 3350
What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3383
3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
Does salt concentration in the food matter when using the DNeasy mericon Food Kit?
FAQ ID - 3346
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit?
FAQ ID - 3348
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K?
FAQ ID - 3362
What is the composition of QIAzol? What is the color of this reagent?
FAQ ID - 3355
Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes (IVD) (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3371
Are PAXgene Blood DNA Tubes (IVD) sterile?
FAQ ID - 3373
What is the additive in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3372
What is the shelf life of PAXgene Blood DNA Tubes (IVD) prior to blood collection?
FAQ ID - 3374
How should blood samples drawn into PAXgene Blood DNA Tubes (IVD) be transported?
FAQ ID - 3375
Can blood samples be frozen in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3377

 

How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at room temperature?
FAQ ID - 3378

Can PAXgene Blood DNA Tubes (IVD) be stored at higher than room temperature (18–25°C)?
FAQ ID - 3380
How long can samples in PAXgene Blood DNA Tubes (IVD) be stored at 2–8°C?
FAQ ID - 3379
Which downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3384
Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
What is the composition of elution buffers used in QIAsymphony DNA Investigator kits?
FAQ ID - 3387
Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323

 

3340 - Which operating system is compatible with PyroMark IdentiFire Software?
3325 - What are the catalog numbers for the packing materials for the QIAcube?
3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?

 

Do I have to use a blood collection set with PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3370
3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
What is the expected DNA yield from 2.5 ml of blood collected in the PAXgene Blood DNA Tube (IVD)?
FAQ ID - 3382
3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
3337 - Which method is used for QIAGEN sequencing service?
3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
3320 - What is the sensitivity of the Type-it HRM PCR Kit?
3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit?
FAQ ID - 3392
3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID -499
What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?
FAQ ID - 3364
3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
I cannot analyze the data from your Investigator HID kits with my GeneMapper software. What can I do?
FAQ ID - 3363
3302 - How long can I store my purified RNA?
Do you sell CoralLoad dye separately?
FAQ ID - 3368
3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?
3195 - Where can I find the Population Data for the Investigator DIPlex kit?
3196 - What is the DIPsorter Freeware?
3188 - How many His residues are required for successful purification on Ni-NTA resins?
3190 - What is the smallest elution volume for RNeasy Mini Spin column?
Is it possible to extract RNA from RNAprotect-stabilized-blood?
FAQ ID -3120
What's the pulsed-field gel electrophoresis (PFGE) conditions of the gel picture showing on your website? http://www.qiagen.com/Search/MagAttract-HMW-DNA-Kit#productdetails
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Is it possible to extract RNA from frozen blood?
FAQ ID -3119
What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit?
FAQ ID -3122
Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
I want to purify RNA from heart tissue. What is the best kit to use?
FAQ ID -3116
The kit says I can use up to 30mg of tissue. When I used 15mg I got a higher yield than when I used 30mg. Why?
FAQ ID -3117
Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
Is it possible to partially use RNeasy 96 plates and use the plate multiple times?
FAQ ID -3124
Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125
Can I use the QIAGEN deparaffinization solution with the IDH 1/2 RGQ PCR kit?
FAQ ID -3100
Can I use stained slide materials with IDH 1/2 RGQ PCR kit?
FAQ ID -3101
Do you have a protocol for obtaining > 20 µg plasmid DNA using the QIAprep spin miniprep kit?
FAQ ID -3102
How to upload a Flexiplate on QIAGEN Webpage?
FAQ ID -3103
How many transfections can be performed with Flexiplate siRNAs?
FAQ ID -3105
What is the standard format for Flexiplate siRNAs delivery?
FAQ ID -3104
Where to look for ordering Custom siRNAs online?
FAQ ID -3106
What is the difference between HiPerFect and HiPerFect HTS?
FAQ ID -3108
Can we do modification XXXXX for custom siRNA that is not on the web page?
FAQ ID -3107
What is the stability of siRNA lyophilized and resuspended?
FAQ ID -3109
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
What are the miScript Controls that can be used with miScript HiSpec Buffer or miScript HiFlex Buffer?
FAQ ID -3111
Do you offer FlexiTube or FlexiPlate siRNAs for rat?
FAQ ID -3114
What is the amplicon size of the PCR product when using the miScript primer assay?
FAQ ID -3156
What are the stopping points in the QIAamp protocol?
FAQ ID -3157
What is the difference between EpiTect Bisulfite Kit and EpiTect Plus Bisulfite Kit?
FAQ ID -3167
What are the benefits of PyroMark Q24 Advanced versus PyroMark Q24?
FAQ ID -3158
Is it possible to use PyroMark Q24 Advanced reagents on PyroMark Q24 system?
FAQ ID -3160
Is it possible to run therascreen mutation or therascreen Pyro kits on PyroMark Q24 Advanced system?
FAQ ID -3161
Is it possible to upgrade existing PyroMark Q24 instruments to PyroMark Q24 Advanced or do I have to purchase a new instrument?
FAQ ID -3162
Do you have data on the DNA yield at different incubation time for the Repli-G Kits?
FAQ ID -3113
How do I perform the PyroMark Q24 Advanced upgrade?
FAQ ID -3163
Which operating system is compatible with PyroMark Q24 Advanced application software?
FAQ ID -3164
What are the differences between new PyroMark Q24 Advanced reagents chemistry compared to PyroMark Q24 Gold reagents?
FAQ ID -3165
What is the miRNA yield from the miRNeasy Serum/Plasma Kit?
FAQ ID -3171
What is the adapted annealing protocol for PyroMark Q24 Advanced procedure?
FAQ ID -3166
What is the RNA size distribution recovered from the miRNeasy Serum/Plasma Kit?
FAQ ID -3172
What is the difference between the 1 nmol FlexiTube and FlexiTube Gene Solution siRNAs?
FAQ ID -3174
What could be a reason for higher viscosity of PCR reaction with STR Plus chemistry?
FAQ ID -3176
Does QIAGEN provide template files for GeneMapper® 5 software?
FAQ ID -3177
Can the Rotor-Gene Q perform gradient PCR?
FAQ ID -3183
Can Rotor-Gene Reports be modified?
FAQ ID -3184
What is the best way to ensure the Rotor-Disc is properly sealed when using the Heat Sealer?
FAQ ID -3179
What is needed for performing delta delta Ct relative quantitation on the Rotor-Gene Q?
FAQ ID -3178
What to do if there is a loss of communication for the Rotor-Gene Q?
FAQ ID -3180
Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q?
FAQ ID -3181
3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates?
Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee?
FAQ ID - 3186
3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns?
3192 - Is QIAGEN Protease compatible with Buffer ATL?
3199 - Which species can be used with the GeneRead rRNA depletion kit?
3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)?
What is the bacterial ribosomal RNA recommended to use in the miRNeasy Serum/Plasma Protocol?
FAQ ID -3173
Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly?
FAQ ID -3136
How do I remove the RNA contamination from purified DNA?
FAQ ID -3133
How can I know whether there is any genomic DNA present in my purified plasmid?
FAQ ID -3134
Is it possible to use RNAprotect Cell Reagent to stabilize buffy coat?
FAQ ID -3139
How do I know whether my miRNA has been successfully purified and how much is expected?
FAQ ID -3135
What is the final concentration of Protease upon reconstitution in the QIAamp DNA Blood Mini?
FAQ ID -3140
Has QIAGEN ever tested Proteinase K treatment for a time period longer than overnight?
Does RNAprotect affect morphology of tissues?
FAQ ID -3142
Is RNAprotect also suitable for the stabilization of miRNA?
FAQ ID -3143
What is the maximum cDNA length that can be synthesized by QuantiTect Reverse Transcription Kit?
FAQ ID -3146
Are miScript primer assays bench-validated?
FAQ ID -3145
What are the minimal and maximum fragment sizes for the QIAquick column in the mericon DNeasy kit?
FAQ ID -3147
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
Can single-stranded DNA be removed by gDNA Eliminator columns?
FAQ ID -3152
How is total RNA purified with RNeasy kits free of genomic DNA contamination?
FAQ ID -3154
Is it possible to quantify protein content in RLT lysates?
FAQ ID -3155
Does RNase-free DNase I affect the isolation of dsRNA?
FAQ ID -3153
What primary cell lines have been tested with HiPerFect Transfection Reagent?
FAQ ID -3137
Is it possible to use the protein resuspended in ALO buffer for downstream BCA assay and Bradford assay?
FAQ ID -3138
What are the miScript Controls that can be used with miScript HiFlex Buffer ONLY?
FAQ ID -3112
How long can I store an RLT lysate?
FAQ ID -3115
What could be a reason for cloudy precipitate in PCR reaction with STR Plus chemistry after cycling protocol is completed?
FAQ ID -3175
Do you have a protocol for the isolation of genomic DNA from yeast/fungi directly from Blood?
FAQ ID -3150
Is REST software Windows 7 compatible?
FAQ ID -3182
Is there cross talk between channels in the Rotor-Gene Q?
FAQ ID -3185
Can QIAzol aqueous phase be used with the gDNA eliminator columns in RNeasy Plus mini kit?
FAQ ID -3151
What is the Spike-in-Control in the miRNeasy Serum/Plasma Kit ?
FAQ ID -3169
What is in the HiSpec Buffer that selectively synthesizes cDNA from mature miRNAs ONLY?
FAQ ID -3168
3197 -Where can I find the Population Data for the Investigator HDplex kit?
How do I prepare the Spike-in-Control catalog # 219610 to get copies/μl?
FAQ ID -3170
Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3416
What is the proper disposal procedure for waste from the sample preparation?
FAQ ID - 3470
Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3415
Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
FAQ ID - 3479
Do you have stability data for RNA isolated with PAXgene miRNA Kit?
FAQ ID - 3480
What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
FAQ ID - 3481
Can the PAXgene Blood DNA Tubes be stored at 4°C?
FAQ ID - 3493
How should I thaw frozen PAXgene Blood DNA Tubes?
FAQ ID - 3495
What is the expected yield from 8.5 ml of blood?
FAQ ID - 3496
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
FAQ ID - 3491
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
FAQ ID - 3498
Does the DNA extracted using the PAXgene Blood DNA System contain RNA?
FAQ ID - 3499
What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?
FAQ ID - 3427
Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
Can I measure virulence factor gene expression?
FAQ ID — 3405
What sample types can I test on qBiomarker Copy Number PCR Arrays?
FAQ ID — 3423
What is the sensitivity for the Microbial DNA qPCR kits?
FAQ ID — 3409
Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
What sequences are used to design the Microbial DNA qPCR Assays?
FAQ ID — 3403
What is the difference between LLOQ and LOD?
FAQ ID — 3402
What is LLOQ?
FAQ ID — 3401
Are the assays species-specific?
FAQ ID — 3400
Why is the copy number call lower than expected?
FAQ ID — 3413
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment?
FAQ ID — 3421
What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3422
What could have gone wrong if the CT values are unusually high for all wells in a sample?
FAQ ID — 3412
How much DNA should I use on a qBiomarker Copy Number PCR Array?
FAQ ID — 3417
How much DNA should I use in a qBiomarker Copy Number PCR Assay?
FAQ ID — 3418
How do you choose which assays to include on the arrays?
FAQ ID — 3420
What testing should be performed in order to assess the quality of a DNA sample?
FAQ ID — 3424
What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?
FAQ ID - 3429
How many samples can be processed with the PAXgene Blood miRNA Kit?
FAQ ID - 3477
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?
FAQ ID - 3460
I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license?
FAQ ID - 3472
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

FAQ ID - 3473

Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
FAQ ID - 3494
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID - 3474
What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID - 3475
Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID - 3476
What is the additive in PAXgene Blood DNA Tubes
FAQ ID - 3486
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?
FAQ ID - 3482
Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
FAQ ID - 3483
Do I have to use a blood collection set with the PAXgene Blood DNA Tube?
FAQ ID - 3484
How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?
FAQ ID - 3489
How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?
FAQ ID - 3492
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?
FAQ ID - 3488
How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?
FAQ ID - 3490
Can the miScript II RT Kit be used to quantify plant miRNAs?
FAQ ID - 3431
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