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Next-generation sequencing

Single-cell sequencing

Image courtesy of Namocell

Maximize insights from minimal sample amounts

Working with single cells, cell populations or low-input DNA or RNA should not limit your ability to uncover a deeper view of the genome or transcriptome. QIAseq Single Cell Library Kits UDI can help you unravel genomic and transcriptomic details from single cells and limited amounts of nucleic acids. Prepare high-complexity whole genome or whole transcriptome libraries using a one-tube workflow with minimal hands-on time. Worried about bias? The PCR-free protocol means you can avoid a negative impact on library diversity from the loss of low-abundance transcripts or GC and length bias. Inclusion of Unique Dual Indices (UDI) minimizes sequencing artifacts due to “index hopping” on Illumina NGS instruments.

Take the QIAseq challenge for single-cell and low-sample input NGS

QIAseq Single Cell DNA Library Kits UDI
  • Whole genome libraries from single cells
    in <4 h
  • Analyze sequence and copy number variations anywhere in the genome
  • Delivers PCR-free NGS libraries and amplified gDNA for follow-up testing
  • REPLI-g DNA polymerase MDA delivers high-fidelity amplification and reduces false-positives
  • Includes Unique Dual Indices to minimize sequencing artifacts due to “index hopping” on Illumina NGS instruments
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QIAseq Single Cell RNA Library Kits UDI
  • Start with single cells, cell populations or enrich for small RNA genomes or viruses
  • Choose to focus on mRNAs or explore the transcriptome
  • REPLI-g RNA amplification and PCR-free protocol reduces bias and provides greater reproducibility
  • Includes Unique Dual Indices which ensure high-quality data from NovaSeq and patterned flow cells
  • NGS-ready libraries from single cells in 5.5 h
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Expand your knowledge by tuning into the webinar

Namocell webinar teaser image

Title: Utilizing RNA and DNA plate-seq workflows for low-input or rare single-cell genomics

Speakers: Felix Alonso-Valenteen Ph.D., Field Application Scientist, Namocell and Ioanna Andreou, Ph.D., Associate Director R&D, NGS Applications Development, QIAGEN

Register now
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Take the fastest way to a single cell with Namocell's single-cell isolation technology.†
Visit the Namocell website†
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Get started with single-cell whole genome library prep using QIAseq Single Cell DNA Library Kits UDI.
Learn more
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Get started with single-cell RNA-seq using the QIAseq Single Cell RNA Library Kits UDI.
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Sample to Insight solution for single-cell and low-input sequencing

single cell infographic

Top 4 considerations for single-cell and low-input NGS

1. Importance of isolation method
Viability and gentle cell handling is mandatory to reveal accurate information from single cells
2. Prerequisites for cell isolation
Cell type, sampling, isolation method and subsequent analysis are important attributes in a complete workflow and must be optimized
3. REPLI-g amplification
QIAseq Single Cell REPLI-g amplification is a superior technology compared to PCR for studying the genome and transcriptome from single cells and low-input DNA or RNA
4. Specific enrichment using QIAseq chemistry
QIAseq Single Cell Library Kits UDI utilize a new buffer composition to allow specific enrichment of small genomes

Related products

  • QIAseq UPX 3’ Targeted RNA Panels
    QIAseq UPX 3’ Targeted RNA Panels

    For ultraplex gene expression analysis using 3' RNA-seq from single cells and low-input samples

    View details

  • Discover the plus
    QIAseq UPX 3’ Transcriptome Kits

    For high-throughput 3' transcriptome analysis from up to 10 ng of purified RNA, cell lysates and single cells

    Learn more

Get in touch

† This link takes you to an external third-party website owned and operated by Namocell, an independent third party unaffiliated with QIAGEN. This link is provided for convenience and informational purposes only. QIAGEN bears no responsibility for and makes no representations or warranties regarding the content of external websites. Please contact the external site directly for answers to any questions regarding its content.