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RNeasy DSP Midi Kit
Cat no. / ID. 78344
50 RNeasy Midi Spin Columns, Elution Tubes (15 mL), RNase-free Reagents and Buffers
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The RNeasy DSP Midi Kit is intended for in vitro diagnostic use.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Fast procedure delivering high-quality total RNA in minutes
- Consistent RNA yields from up to 10 mL human blood or 3 mL bone marrow aspirates
- Ready-to-use RNA for high performance in any downstream application
- No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
- RNA purification system for use with other QIAGEN IVD products
Product Details
The RNeasy DSP Midi Kit uses silica-membrane technology (RNeasy technology) for manual isolation and purification of total RNA from human blood and bone marrow aspirates for use with in-vitro diagnostic QIAGEN products that list the RNeasy Midi Kit as an accessory.
Principle
The RNeasy DSP Midi Kit uses well-established RNeasy technology for purifying RNA. RNeasy technology simplifies total RNA isolation by combining the stringency of a guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification.
Procedure
With the RNeasy DSP Midi Kit, total RNA is easily purified from human blood or bone marrow aspirates. Erythrocytes from blood are lysed (erythrocyte lysis buffer not included in the kit, Buffer EL, cat. no. 79218) and white blood cells are collected, lysed, and homogenized. Ethanol is added to provide ideal binding conditions and the lysate is then loaded onto the RNeasy Midi column. Subsequent washing and centrifugation steps are applied before RNA is eluted in RNase-free water. Detailed protocols can be found in the instructions for use of the corresponding downstream applications.
Applications
RNA purified using the RNeasy DSP Midi kit can either be directly used for sensitive analytical detection in downstream assays such as qPCR or RNA can further be concentrated using the RNeasy DSP MinElute Cleanup Kit. The specific procedure can be found in the instructions for use of the corresponding downstream applications.
Specifications
| Features | Specifications |
|---|---|
| Applications | Downstream detection procedures in molecular diagnostics, such as PCR |
| Elution volume | 300-500 µL |
| Format | Spin column |
| Sample type | Human blood and bone marrow aspirates |
| Processing | Manual (centrifugation) |
| Technology | Silica technology |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Total RNA |
Resources
Safety Data Sheets (1)
Certificates of Analysis (1)
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