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EZ1 Advanced XL, Priority Package
Cat no. / ID. 9001875
Instrument and service agreement package: robotic workstation for automated purification of nucleic acids from up to fourteen samples using EZ1 Kits; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits.
Service optionService Options
Priority Package
System
Priority Package Plus
System PRV-2
EZ1 Advanced Instruments are intended to be used only in combination with QIAGEN kits indicated for use with EZ1 Advanced Instruments for the applications described in the kit handbooks.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- High-quality genomic DNA and RNA from a range of sample materials
- Viral RNA/DNA and bacterial DNA from biomedical samples
- Throughput of up to 14 samples per run
- Effortless data management with full traceability
- UV lamp to help minimize contamination risk
Product Details
Performance
Standardization and traceability
The EZ1 Advanced XL sets new standards in data management. Barcode reading enables complete tracking of samples and reagents throughout the entire purification process. Notes and assay identification information can also be easily entered. For increased process control and reliability, reagent identification and lot numbers are logged together with the expiration date, and a warning is issued if the expiration date has been exceeded. The run report, automatically generated at the end of each protocol, contains all relevant instrument information, including maintenance procedures and decontamination data. The instruments generate a pdf and a csv file after each run. Data can be easily transferred to a printer or computer within the network and the report file can be subsequently processed by a LIMS (Laboratory Information Management System).
Proven technology with improved performance
Proven EZ1 Kits, in combination with EZ1 Advanced XL Cards, ensure high-performance results in purification procedures. The unrivaled ease of use of the EZ1 Advanced XL enables fast and reproducible purification, without the need for specialized training or previous experience with laboratory automation. The extensive application range, including DNA purification from blood, tissues, and human identity and forensic samples, RNA purification from cells and tissues, and purification of viral nucleic acids from a range of sample types, delivers the flexibility that is essential for laboratories.
Safety and reliability
The EZ1 Advanced XL has been designed to ensure high process safety, high performance, and user convenience. A sensor ensures that the instrument door remains closed during sample preparation, reducing contact of laboratory personnel with potentially infectious samples and protecting sample integrity. At the end of each run, an intelligent-design UV lamp decontaminates the inner surface of the instrument. UV decontamination is highly effective in eliminating Gram-positive and Gram-negative bacteria (see tables). If required, the instrument can be placed in a safety cabinet, providing increased user safety.
| Irradiation (min) | Run | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|---|
| 30 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 30 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
| 30 | 3 | 10 | 0 | 0 | 0 | 0 | 3 |
| 30 | Control | 311 | 352 | 367 | 367 | 351 | 396 |
| 60 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 60 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| 60 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| 60 | Control | 348 | 307 | 326 | 326 | 396 | 385 |
| Irradiation (min) | Run | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|---|
| 30 | 1 | 12 | 10 | 17 | 2 | 6 | 4 |
| 30 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| 30 | 3 | 10 | 0 | 2 | 11 | 0 | 3 |
| 30 | Control | 248 | 256 | 396 | 314 | 321 | 267 |
| 60 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 60 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| 60 | 3 | 10 | 0 | 0 | 0 | 0 | 0 |
| 60 | Control | 264 | 305 | 368 | 292 | 347 | 388 |
Principle
Automated sample preparation enables efficient, standardized purification of nucleic acids. Manual sample handling is minimized, ensuring safety to the user and reliable processing of samples.
The EZ1 Advanced XL instrument uses proven EZ1 Kits for purification of DNA, RNA, and viral nucleic acids from a wide range of starting materials. Purified nucleic acids are ready for immediate use in downstream applications. The combination of an easy-to-use instrument, with error-free protocol selection and worktable setup, and prefilled, sealed reagent cartridges makes nucleic acid purification easy.
Procedure
Flexibility and ease of use
The self-contained EZ1 Advanced XL instrument ensures optimal ease of use and walkaway automation. A wide range of protocols is available for purification of DNA, RNA, or viral nucleic acids. Up to 14 samples can be processed per run in as little as 20 minutes. The instrument is operated via a keypad, and instrument information is displayed on the VFD (vacuum fluorescent display).
All processing steps are performed by the instruments — from piercing reagent cartridges to elution of pure nucleic acids. A separate computer is not required for operation, and EZ1 Kits include all reagents and accessories required to process your samples. EZ1 Kits provide prefilled, foil-sealed reagent cartridges that remain sealed until the instrument door is closed and the protocol run started, reducing the risk of contamination during setup.
If desired, up to 4 EZ1 Advanced XL instruments can be connected to an external computer, expanding throughput up to 56 samples per run. This enables you to easily adapt your laboratory setup to changing sample preparation requirements.
EZ1 Kits and EZ1 Advanced XL Cards for a wide range of applications
The EZ1 Advanced XL uses proven EZ1 Kits with preprogrammed protocols on EZ1 Advanced XL Cards. EZ1 Kits are available for a broad range of applications including work in human identity and forensics, biomedical research, and gene expression analysis (see table).
| Card | Kit | Sample |
|---|---|---|
| EZ1 Advanced XL DNA Blood Card | EZ1 DNA Blood 200 µl Kit | Blood and blood-derived samples |
| EZ1 Advanced XL DNA Blood Card | EZ1 DNA Blood 350 µl Kit | Blood and blood-derived samples |
| EZ1 Advanced XL DNA Dried Blood Card | EZ1 DNA Tissue Kit | Dried blood |
| EZ1 Advanced XL DNA Buffy Coat Card | EZ1 DNA Blood 350 µl Kit | Buffy coat |
| EZ1 Advanced XL DNA Tissue Card | EZ1 DNA Tissue Kit | Tissues |
| EZ1 Advanced XL DNA Paraffin Section Card | EZ1 DNA Tissue Kit | Paraffin-embedded tissues |
| EZ1 Advanced XL DNA Buccal Swab Card | EZ1 DNA Tissue Kit | Buccal swabs |
| EZ1 Advanced XL DNA Investigator Card | EZ1 DNA Investigator Kit | Forensic and human identity samples |
| EZ1 Advanced XL DNA Bacteria Card | EZ1 DNA Tissue Kit | Human samples, swabs, biopsies, bacterial cultures |
| EZ1 Advanced XL Virus Card v2.0 | EZ1 Virus Mini Kit v2.0 | Viral DNA and RNA and bacterial DNA from serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swab samples |
| EZ1 Advanced XL RNA Card | EZ1 RNA Universal Tissue Kit | Any type of human or animal tissue |
| EZ1 Advanced XL DSP DNA Blood Card* | EZ1 DSP DNA Blood Kit* | Human whole blood |
| EZ1 Advanced XL DSP Virus Card* | EZ1 DSP Virus Kit* | Viral DNA and RNA and bacterial DNA from serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swab samples |
Applications
Nucleic acids purified using the EZ1 Advanced XL are ready for use in a wide range of downstream applications, including:
- Oncology, forensics, and genetic identity testing
- Genotyping
- Infectious disease research, including bacterial genotyping
- Gene expression analysis
Services
Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?
If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!
QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.
Learn more about trade-in/trade-up opportunities.
Supporting data and figures
Simple protocol.
1. Insert the EZ1 Advanced XL Card with the desired protocol into the card slot and start the instrument. 2. Place samples and prefilled, sealed reagent cartridges into the instrument. Samples are processed automatically and purified nucleic acids are transferred to elution tubes. 3. Remove pure nucleic acids in 1.5 ml microcentrifuge tubes at a concentration suited to the downstream application.
Specifications
| Features | Specifications |
|---|---|
| Features | Touch LCD user interface, IC card with protocols/software, barcode reader, UV lamp |
| Pipetting range | 50-1000 µl |
| Kits designed for this instrument | QIAGEN EZ1 Kits |
| Technology | Magnetic-particle technology |
| Instrument dimensions | 510 x 510 x 570 mm (w x d x h) |
| Technical data of the instrument | Weight 48 kg, 100–240 V AC, 50–60 Hz |
| Protocols/main application on this instrument | Purification of DNA, mRNA, total RNA, and viral RNA and DNA |
| Samples per run (throughput) | 1–14 samples per run |
Resources
User-Developed Protocols (2)
Instrument User Manuals (10)
Brochures & Guides (3)
Application Notes (3)
Supplementary Protocols (4)
Kit Handbooks (1)
Validation Reports (1)
Certificates of Analysis (1)
FAQ
What to do if I get error 24 (Open door in motion) on my EZ1 instruments?
What is the lifetime of the UV lamp on an EZ1 Advanced and EZ1 Advanced XL?
I observe more than normal magnetic bead carryover in my eluates after extraction on EZ1 instruments. What should I do?
What to do if I get error 29 (Backup memory on the CPU is destroyed) on my EZ1 instrument?
What to do if I get error 25 (Abnormal input from bottom sensor in motion) on my EZ1 instrument?
I get “Master Data Error” after turning on my EZ1 instrument? What should I do?
What is the part number for transport lock or knurled knob for packing the EZ1 Advanced XL instrument?
What to do if I get error 13 (Failed to return to P axis, protocol in run) on my EZ1 instrument?
What to do if I get error 14 (Failed to return to M axis, protocol in run) on my EZ1 instrument?
What to do if I get error 19 (Y axis end limit, protocol in run) on my EZ1 instrument?
I get “UV lamp did not ignite error” on my EZ1 Advanced and EZ1 Adavanced XL. What should I do?
What to do if I get error 0182 (Attempt to move beyond the z-m axis, interference prevention value) on my EZ1 instrument?
What is the part number for silicone grease for greasing the O-ring of tip adapters on EZ1 series of instruments?
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