GeneRead QIAcube

ライブラリー・テンプレート調製の自動化および能率化

Products

GeneRead QIAcube は研究用です。疾病の診断、治療または予防の目的には使用することはできません。日本での販売は、GeneReader NGS Systemとして、GeneReader Platform、GeneReader QIAcube、QCI Analyzer などを含めたパッケージ販売となっております。資料請求・お問い合わせは、弊社テクニカルサポート techservice-jp@qiagen.com までお願いいたします。

GeneRead QIAcube (230 V)

Cat. No. / ID: 9002345

Robotic workstation for automation of clonal amplification: includes installation and training, 1 year warranty on parts and labor

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GeneRead QIAcube (110 V)

Cat. No. / ID: 9002344

Robotic workstation for automation of clonal amplification: includes installation and training, 1 year warranty on parts and labor

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GR QIAcube, Premium Agreement

Cat. No. / ID: 9243848

On-site GeneRead QIAcube instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.

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GR QIAcube, Full Agreement

Cat. No. / ID: 9243846

On-site GeneRead QIAcube instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.

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GR QIAcube, Preventive Subscription

Cat. No. / ID: 9243850A

One on-site Preventive Maintenance or Inspection Service visit for the GeneRead QIAcube, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

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Features

顕著な手作業時間の短縮と再現性の増大
シーケンス用ライブラリー・テンプレート同時調製が1～4 つの間でスケール化可能
直感的なタッチスクリーンでセットアップを通じてガイド
実績のあるQIAcube プラットフォームをベースにした装置
GeneReader NGS System で実証済みのバフォーマンス

Product Details

GeneRead QIAcube は、次世代シークエンシング用のテンプレート調製におけるクローナル増幅ステップをお手頃なお値段で自動化できます。場所をとらず直感的なユーザーインターフェイスを利用したGeneRead QIAcube は、手作業時間を40 分まで短縮し、複数のライブラリー・プールを同時に調製可能です。

Principle

GeneRead QIAcube は、GeneRead Clonal Amp Q Kit を用いてクローナル増幅を自動化するためにデザインされています。GeneRead QIAcube は、遠心機、ヒートシェーカー、マグネットステーション、ピペッティングシステム、自動グリッパーを含む内装装置を制御します。GeneRead QIAcube は、GeneReader NGS System 上で検証済みです。

Procedure

GeneRead QIAcube は、直感的な内蔵GUI により、プロトコールの選択およびデータ保存を可能にします。お客様による追加のドキュメンテーションは不要です。GeneRead QIAcube の作動中は、タッチスクリーンにプロトコールの現状情報およびプロセス終了までの残り時間が示されます。本装置は、1 回のランで4 ライブラリー・プールの調製を実現します。4 ライブラリー・プールのテンプレート調製では手作業に要する時間は40分まで短縮できます。4 x 10 plex を用いてアウトプット能力をランあたり40 サンプルまで増加できます。尚、別途Deep Well Block が利用可能なPCR 装置が必要となります。

Applications

GeneRead QIAcube は、GeneReader NGS System プラットフォーム用にテンプレート調製に相当する工程を自動化するためにデザインされています。

Resources

Download Safety Data Sheets for QIAGEN product components.

FAQ

For an up-to-date list of available protocols, visit QIAcube Standard Protocols.

Click here for a virtual demonstration on how the QIAcube operates.

FAQ ID -1403

Yes. The heated shaker on the QIAcube can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the shaker, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.7 'Operating the shaker'.

The shaker can be used to heat and shake samples simultaneously, shake samples without heating, or heat samples without shaking.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a virtual demonstration of the heater and shaker on the QIAcube.

FAQ ID -1422

Yes. The QIAcube centrifuge can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the centrifuge, refer to the QIAcube User Manual, chapter 5 'Operating Procedures, section 5.8 'Operating the Centrifuge'.

FAQ ID -1423

Yes, this is absolutely necessary. If the lids are not securely fixed in the rotor adapter slots during processing on the QIAcube, they will become detached from the elution tubes and columns during centrifugation and will collect at the bottom of the centrifuge chamber.

FAQ ID -1415

The following disinfectants and detergents are recommended for cleaning the QIAcube:

Mikrozid® Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g Mikrozid Liquid)
RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door
RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)
Note: Alcohol is not recommended for cleaning of the door.

Europe

Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15–30% nonionic surfactants).

USA

DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride).

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

FAQ ID -1416

No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.

See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a demonstration on how the QIAcube is used with the RNeasy Mini Kit for automated purification of RNA.

FAQ ID -1406

No, it is not possible to connect the QIAcube with laboratory computer networks.

FAQ ID -1411

The QIAcube has the following weight and dimensions:

Weight: 70.5 kg (155.4 lb.)
Width: 65 cm (25.6 in.)
Depth: 62 cm (24.4 in.)
Height (door closed): 57 cm (22.4 in.)
Height (door open): 81 cm (32 in.)

FAQ ID -1407

Please refer to section 6.5 'Decontaminating the QIAcube' in chapter 6 'Maintenance Procedures' of the QIAcube User Manual.

FAQ ID -1419

Yes, rotor and/or buckets can be removed from the QIAcube for cleaning. For information on how to remove these parts, refer to the QIAcube User Manual.

FAQ ID -1410

You can stop a QIAcube protocol if there is an emergency by pressing “Cancel”. To confirm that you want to stop the protocol run, press “OK”. To cancel the 'stop protocol' command, press “Cancel”.

Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.

FAQ ID -1414

The corresponding manual kits can be used for all available protocols on the QIAcube. The manual kits contain sufficient buffers and reagents to process the number of spin columns provided with the kit.

In addition to the purification kit, Rotor Adapters (10 x 24), cat. no 990394, and the required disposable tips:

Filter-Tips, 200 µl (1024), cat no. 990332,
Filter-Tips, 1000 µl (1024), cat no. 990352,
Filter-Tips, 1000 µl wide-bore (1024), cat no. 990452,

need to be ordered separately. To find out which filter-tips are required for a specific application, refer to the corresponding protocol sheet.

FAQ ID -1402

No. Autoclaving the rotor adapters will cause the plastic to deform and the rotor adapters will no longer fit properly into the centrifuge buckets.

FAQ ID -1409

No. The QIAcube is equipped with an optical sensor, which determines the tip size and the number of tips loaded onto the worktable prior to starting a run.

The QIAcube can use partially used tip racks from previous runs, provided that the racks contain sufficient tips to process the number of samples loaded for the current run.

FAQ ID -1418

Yes. At the end of each protocol run on the QIAcube, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.

FAQ ID -1412