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PCR/qPCR/dPCR

特異的かつ高感度、堅牢なPCRを確実に

遺伝子増幅を行う際に、実験の成功はスループット、アッセイ感度、テンプレートの難易度に起因しない反応のロバストネス、データ正規化の信頼性など、いくつかの重要な要素に左右される可能性があります。使用するPCRメソッドに関連する特定の課題を克服することが必要になる場合もあります。

当社は、お客様がどのメソッドを選択しようと、エンドポイントPCR、qPCR、dPCR用に最適化された豊富なソリューションを提供します。当社のキット、アッセイ、装置、および付属品は、お客様がターゲットを確実に増幅および検出し、ほぼすべてのアプリケーションのワークフローを合理化するのに役立ちます。

  • Measure gene expression (mRNA, miRNA)
  • Quantify DNA or RNA targets
  • Detect & quantify pathogen and/or viral RNA/DNA
  • Quantify residual DNA
  • Convert RNA to cDNA for quantification
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  • Mutation detection (including rare/ultra-rare variants)
  • Analyze copy number variation (CNV)
  • Detect viral load
  • Gene expression quantification
  • Quantify low-abundance targets
  • Measure residual DNA
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  • Amplify DNA for cloning or sequencing
  • Qualitative pathogen detection (presence/absence)
  • CRISPR screening
  • Genotyping
  • Convert RNA to cDNA for storage
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  • Cq/Ct values
  • Relative quantification (ΔΔCq) or absolute quantification from standards
  • cDNA for downstream use
  • Absolute target quantification (copies/µL reaction)
  • Fractional abundance (%)
  • Mutation/target frequency
  • Copy number per genome
  • Amplified DNA or cDNA product
  • Band visualization on gel
  • Presence/absence result
  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
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  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Purified samples recommended
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  • Isolated DNA or cDNA
  • Isolated RNA
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  • Quantitative real-time PCR
  • Reverse transcription
  • Amplification and fluorescence-based detection in real-time for quantification
  • Quantitative digital PCR
  • Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
  • Standard end-point PCR
  • DNA amplification and visualization (e.g., gel-based) and downstream applications
  • When reliable quantification is required over a wide linear range
  • When standard curve-based or relative quantification is sufficient
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  • When precise quantification is required
  • When detecting rare alleles or small fold changes
  • When standard curves are undesirable
  • When working near the detection limit (low-abundance targets)
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  • When qualitative detection without quantification is sufficient
  • When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
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  • Real-time PCR thermal cycler
  • Dye-based (e.g., SYBR Green) or probe-based detection
  • QIAcuity dPCR platform
  • Probe‑based or intercalating dye detection
  • Thermal cycler
  • Visualization (e.g., via agarose gel electrophoresis)
閲覧 PCR/qPCR/dPCR

製品

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DNA methylation analysis
156個の製品が見つかりました
QIAcuity High Multiplex Probe PCR Kit
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
ID SOLUTIONS IDNAPTEX dPCR Assays
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAprep&amp Plasmodium Kit
QIAprep&amp Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep&amp Plasmodium Assays
QIAprep&amp Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
QIAcuity RCL Quant Kit
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
QIAGEN OneStep RT-PCR Kit
QIAGEN OneStep RT-PCR Kit
高感度で特異的な1ステップRT-PCR
QIAGEN Multiplex PCR Kit
QIAGEN Multiplex PCR Kit
至適化なしに特異性および感度の高いマルチプレックスPCR
QuantiTect Reverse Transcription Kit
QuantiTect Reverse Transcription Kit
遺伝子発現解析のための高感度な2ステップリアルタイムRT-PCR用cDNAの迅速合成
HotStarTaq DNA Polymerase
HotStarTaq DNA Polymerase
最小限の至適化で特異性の高い増幅反応
RT2 Profiler PCR Arrays
RT2 Profiler PCR Arrays
信頼性と感度の高い遺伝子発現プロファイリング
REPLI-g Single Cell Kit
REPLI-g Single Cell Kit
シングルセルあるいは限られたサンプル量からの全ゲノム増幅(WGA)
QuantiTect RT-PCR Kit
QuantiTect RT-PCR Kit
SYBR® Green Iを用いる1ステップqRT-PCR、遺伝子発現分析用の配列特異的プローブを用いる1ステップqRT-PCRおよびマルチプレックスqRT-PCR用