PCR/qPCR/dPCR
Spezifische, sensitive, robuste und zuverlässige PCR
Bei der Durchführung von Genamplifikationen kann der Erfolg Ihrer Experimente von mehreren kritischen Faktoren abhängen, einschließlich Durchsatz, Assay-Sensitivität, Robustheit der Reaktion auch bei schwierigen Templates und zuverlässiger Datennormalisierung. Sie müssen möglicherweise auch die besonderen Herausforderungen in Verbindung mit der verwendeten PCR-Methode überwinden.
Egal welche Methode Sie verwenden, wir bieten eine Fülle optimierter Lösungen für Endpunkt-PCR, qPCR und dPCR an. Unsere Kits, Assays, Geräte und unser Zubehör helfen Ihnen, Ihre Targets zuverlässig zu amplifizieren und nachzuweisen, und ermöglichen die Optimierung Ihres Workflows für praktisch jede Applikation.
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
Durchsuchen PCR/qPCR/dPCR
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DNA methylation analysis
156 Produkte gefunden
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
QIAGEN OneStep RT-PCR Kit
For highly sensitive and specific one-step RT-PCR
QIAGEN Multiplex PCR Kit
For highly specific and sensitive multiplex PCR without optimization requirements
QuantiTect Reverse Transcription Kit
For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis
HotStarTaq DNA Polymerase
For highly specific amplification with minimal optimization
RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis using laboratory-verified assays
REPLI-g Single Cell Kit
For highly uniform whole genome amplification (WGA) from single cells or limited sample material
QuantiTect-RT-PCR-Kits
Für Ein-Schritt-qRT-PCR mit SYBR® Green I und Ein-Schritt-qRT-PCR und Multiplex-qRT-PCR mit sequenzspezifischen Sonden für die Genexpressionsanalyse
RT2 SYBR® Green qPCR Mastermixes
For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
HotStarTaq Master Mix Kit
For highly specific amplification for any PCR application
QuantiTect-PCR-Kits
Für qPCR und Zwei-Schritt-qRT-PCR mit SYBR Green I und für qPCR, Zwei-Schritt-qRT-PCR und auch Multiplex-qPCR und Zwei-Schritt-qRT-PCR im Multiplex-Format mit sequenzspezifischen Sonden
Rotor-Gene Q
For outstanding performance in real-time PCR
QuantiFast Pathogen +IC Kits
Für sensitiven und zuverlässigen Nachweis von viraler RNA/DNA und bakterieller DNA, einschließlich interner Kontrolle
HotStarTaq Plus DNA Polymerase
For fast and highly specific amplification in all applications
miRCURY LNA SYBR® Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Taq DNA Polymerase
Taq DNA Polymerase delivers fast PCR set up with minimal optimization for efficient amplification of routine, low-copy, longer or difficult targets
QuantiTect Primer Assays
For SYBR® Green-based expression analysis of individual genes using bioinformatically-verified assays
miRCURY LNA miRNA PCR Assays
Für die hoch sensitive und spezifische miRNA-Quantifizierung mit LNA-optimierter, SYBR® Green basierter miRNA-PCR
miRCURY PCR Controls
Validated primer sets for normalization of miRNA expression levels in real-time PCR analyses
RT2 First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays
miRCURY LNA RT Kit
Für eine optimale cDNA-Erststrangsynthese mit miRCURY LNA miRNA PCR Systemen auf Basis von SYBR Green oder Hydrolysesonde
HotStarTaq Plus Master Mix Kit
For fast and highly specific amplification in all applications
Custom Microbial DNA qPCR Arrays
For real-time PCR-based, customized microbial identification or profiling
QuantiTect Virus Kits
Für den hoch sensitiven Nachweis von viraler RNA und/oder DNA
RT2 qPCR Primer Assays
For accurate and reliable gene expression analysis using laboratory-verified assays
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
QuantiNova-PCR-Kits
Für hoch sensitive, spezifische und ultraschnelle Real-time- und Multiplex-PCR auf Sondenbasis und unerreichte Ergebnisse bei der qPCR auf SYBR Green-Basis
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
Omniscript RT Kit
For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
Custom RT2 Profiler PCR Arrays
For reliable and sensitive gene expression profiling using laboratory-verified assays
Microbial DNA qPCR Assays
For real-time PCR-based microbial detection; includes one assay with Microbial qPCR Mastermix for 100 reactions
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
QuantiNova Reverse Transcription Kit
For fast cDNA synthesis and reproducible real-time two-step RT-PCR
QuantiNova Pathogen +IC Kit
Für den ultraschnellen, gleichzeitigen Nachweis von viraler RNA/DNA und bakterieller DNA, einschließlich interner Kontrolle
miRCURY LNA miRNA Custom PCR Panels
Benutzerdefinierte 96- und 384-Well-Platten mit LNA-optimierten, SYBR® Green-basierten miRNA qPCR-Assays
Taq PCR Master Mix Kit
For convenient PCR setup using a premixed solution
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QuantiNova-RT-PCR-Kits
Für die hoch sensitive und spezifische Ein-Schritt-qRT-PCR und Multiplex-qRT-PCR mit sequenzspezifischen Sonden oder die Ein-Schritt-qPCR mit SYBR® Green I zur Genexpressionsanalyse
QuantiFast SYBR® Green RT-PCR Kit
For fast, one-step qRT-PCR using SYBR Green I for gene expression analysis
Taq PCR Core Kit
For standard and specialized PCR applications, includes dNTP mix
miRCURY LNA miRNA Focus PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
FastLane Cell RT-PCR Kits
For SYBR® Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
REPLI-g Advanced DNA Single Cell Kit
For whole genome amplification (WGA) from single eukaryotic cells, limited samples or purified genomic DNA
miRCURY LNA miRNA miRNome PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
QIAGEN Fast Cycling PCR Kit
For ultrafast and specific PCR on any thermal cycler
Sensiscript RT Kit
For reverse transcription of less than 50 ng RNA per reaction for end-point PCR
REPLI-g WTA Single Cell Kit
For whole transcriptome amplification of total RNA or mRNA from single cells
miScript miRNA Mimics
Für Studien zur miRNA-Funktion und Genregulierung mit synthetischer miRNA
Microbial DNA qPCR Arrays
For real-time PCR-based, application-specific microbial identification or profiling
qBiomarker Somatic Mutation PCR Arrays
For rapid and accurate profiling of the somatic DNA mutation status of gene panels
QIAGEN OneStep Ahead RT-PCR Kit
For faster one-step RT-PCR with high sensitivity, specificity and fidelity
QuantiTect Whole Transcriptome Kit
For unlimited real-time PCR analysis from precious RNA samples
qBiomarker Somatic Mutation PCR Assays
For detecting the presence of specific DNA sequence mutations in cancer and oncogenesis
REPLI-g Cell WGA & WTA Kit
For parallel whole genome and whole transcriptome amplification from cells and limited samples
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
Microbial DNA qPCR Assay Kits
For real-time PCR-based microbial detection; includes one assay and everything required for a successful qPCR run for 20 reactions
miRCURY LNA miRNA Custom PCR Assays
For SYBR® Green qPCR-based miRNA quantification using LNA-optimized miRNA PCR primer sets
miRCURY LNA miRNA PCR Starter Kit
Für den schnellen Einstieg in miRNA-Quantifizierungsexperimente mit SYBR® Green basiertem qPCR-Nachweis
RT2 SYBR® Green FAST Mastermixes
For use with 100-ring disc format RT2 Profiler and RT2 lncRNA PCR Arrays on the Rotor-Gene Q
RT2 PreAMP cDNA Synthesis Kit
For preamplification of cDNA from small or FFPE samples of RNA for analysis on RT2 Profiler PCR Arrays
FastLane Cell cDNA Kit
For high-speed preparation of cDNA without RNA purification for use in real-time RT-PCR
UCP Multiplex PCR Kit
Ultra-Clean Production master mix for multiplex hot-start PCR and microbiome applications
REPLI-g FFPE Kit
For direct whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
qBiomarker Copy Number PCR Assays
For locus-specific analysis of copy number variations and alterations
RT2 lncRNA PCR Arrays
For lncRNA expression analysis by pathway and disease using laboratory-verified SYBR® Green qPCR assays
REPLI-g Mitochondrial DNA Kit
For highly uniform whole genome amplification from human and non-human mitochondrial DNA
QIAGEN PCR Cloning Kits
For direct cloning of PCR products generated by Taq DNA polymerases
RNA Spike-In Kit, For RT
For quality control of the RNA isolation and cDNA synthesis steps of miRCURY LNA miRNA PCR experiments
Type-it Mutation Detect PCR Kit
For accurate and reliable multiplex PCR analysis of mutations
Certal Residual DNA Detection Kits
Für den sensitiven sondenbasierten Nachweis von Rest-DNA aus CHO-Wirtszellen, einschließlich einer internen Kontrolle
RT2 HT First Strand Kit
For cDNA synthesis and genomic DNA elimination in 96 RNA samples for use with RT2 Profiler PCR Arrays
Custom qBiomarker Somatic Mutation PCR Arrays
For rapid and accurate profiling of somatic mutations using a custom gene panel
miRCURY LNA miRNA QC PCR Panel
Für die schnelle und einfache Qualitätskontrolle von miRNA-Proben vor dem SYBR® Green-basierten qPCR-Profiling
RT2 lncRNA qPCR Assay
For long noncoding gene expression analysis by real-time PCR
XpressRef Universal Total RNA
For use as a positive control for gene expression detection methods
QIAsymphony mericon Bacteria Kit
For automated isolation of pathogen DNA from primary bacterial enrichment cultures
UCP HiFidelity PCR Kit
Ultra-Clean Production master mix for high-fidelity hot-start PCR and microbiome applications
RT2 Profiler PCR Array Modification
For gene expression profiling using a partially modified pathway or disease panel
RT2 PreAMP Pathway Primer Mixes
For amplification of cDNA templates prior to analysis on RT² Profiler PCR Arrays
RT2 Easy First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays
Microbial DNA qPCR Multi-Assay Kits
For real-time PCR-based, application-specific microbial identification or profiling; includes 3–6 assay tubes in one box for 20 reactions
Rotor-Gene SYBR® Green PCR Demo Kit
For evaluation of Rotor-Gene Q performance
Microbial DNA Positive Control
For use as a positive control for Microbial DNA qPCR Assays
mericon Pathogen Detection Assay
For real-time PCR detection of pathogen DNA in food or animal feed
qBiomarker SYBR® Green Mastermixes
For use with qBiomarker Copy Number PCR Assays and Arrays
Custom RT2 PreAMP Pathway Primer Mixes
For amplification of cDNA templates prior to analysis on Custom RT² Profiler PCR Arrays
RNA QC PCR Arrays
For quality assessment of RNA samples before characterization using PCR arrays
RT2 qPCR Primer gDNA Controls
For detection of genomic DNA contamination in RNA and cDNA
Supplemental Microbial qPCR Mastermix (ROX)
For real-time PCR applications
RT2 lncRNA PreAMP Primer Mixes
For cDNA synthesis prior to real-time PCR
mericon MeatTracker Kit
For detection of animal DNA in food, animal feed or pharmaceutical samples using real-time PCR
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
Supplemental Microbial qPCR Mastermix (Fluorescein)
For real-time PCR applications
Haushaltsgene (housekeeping genes)
Zur Verwendung bei der Normalisierung Ihres Experiments zur Erstellung relativer Genexpressionsprofile
miRCURY LNA miRNA Custom Bulk Plate
Customer-configurable SYBR® Green-based miRNA qPCR Assays in convenient plate format
miRCURY LNA miRNA Custom Probe PCR Assays
Für eine extrem sensitive und präzise Quantifizierung reifer miRNA mit LNA-optimierter, sondenbasierter qPCR
miRCURY LNA miRNA Probe PCR Assays
Für eine extrem sensitive und präzise Quantifizierung reifer miRNA mit LNA-optimierter, sondenbasierter qPCR
miRCURY LNA Probe PCR Kits
Für eine leistungsstarke PCR mit dem miRCURY LNA miRNA Probe PCR-System
qBiomarker Copy Number PCR Arrays
For profiling copy number variations and alterations
QIAcuity Probe PCR Kit
Zur Verwendung mit den QIAcuity digitalen PCR-Geräten
QIAcuity UCP Probe PCR Kit
Kits aus Ultra Clean Production zur Verwendung mit den QIAcuity Geräten für die digitale PCR
QIAcuity OneStep Advanced Probe Kit
Für Ein-Schritt-RT-PCR auf den QIAcuity Geräten für die digitale PCR
QIAcuity One-Step Viral RT-PCR Kit
Zur Verwendung mit den QIAcuity digitalen PCR-Geräten
QIAcuity Residual DNA Quantification Kits
Zur Überprüfung auf Verschleppung von Wirtszell-DNA mit höherer Genauigkeit und Präzision unter Verwendung des QIAcuity Digital PCR Systems