Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted by relevance What are the SureSilencing shRNA Plasmids? FAQ ID -2784 View What criteria should one use in choosing between siRNA versus shRNA for their studies? FAQ ID -2771 View What does a typical EpiTect Methyl qPCR Assay or Array include? FAQ ID -2740 View Why did the real-time PCR yield Ct values < 12? FAQ ID -2727 View Why can't I find the DNA methylation qPCR Assay for my gene of interest? FAQ ID -2743 View What is the composition of Buffer RPE? FAQ ID -2797 View Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays? FAQ ID -2713 View What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products? FAQ ID -2715 View Which qPCR instrument should I use with your RT² qPCR Primer Assays? FAQ ID -2714 View Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries? FAQ ID -2710 View Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be? FAQ ID -2717 View What is the composition of Buffer S3? FAQ ID -2788 View What MOI should I use for my cells? FAQ ID -2770 View What RT² qPCR Primer Assays are available? FAQ ID -2708 View What transfection reagents are compatible with the Cignal Reporter Assays? FAQ ID -2764 View What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture? FAQ ID -2777 View What is included in the SureSilencing shRNA Plasmids shipment? FAQ ID -2785 View What is the composition of Buffer RLC? FAQ ID -2795 View What is the composition of Buffer RLT? FAQ ID -2793 View What is the composition of Buffer RLT plus? FAQ ID -2794 View What is the composition of Buffer BB? FAQ ID -2790 View What is the composition of Buffer ETR? FAQ ID -2789 View What is the composition of Buffer PB? FAQ ID -2791 View What are the key parameters contributing to unwanted off-target effects in an RNAi experiment? FAQ ID -2775 View What are the structures of the siRNA molecules used in RNAi studies? FAQ ID -2760 View What are the critical factors in designing the siRNA molecules to be used for RNAi studies? FAQ ID -2759 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View What is the composition of gDNA Eliminator Solution? FAQ ID -2799 View What is the composition of Buffer RW1? FAQ ID -2796 View What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays? FAQ ID -2739 View What is the composition of Buffer RWT? FAQ ID -2798 View Can miScript miRNA qPCR Assays be used for pre-miRNA detection? FAQ ID -2724 View Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs? FAQ ID -2723 View Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array? FAQ ID -2736 View Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays? FAQ ID -2725 View Do you always run samples in triplicates? FAQ ID -2703 View Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit? FAQ ID -2722 View Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids? FAQ ID -2787 View Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides? FAQ ID -2737 View Can I make stable cell lines with the Cignal Reporter Assays? FAQ ID -2763 View Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector? FAQ ID -2783 View How are the primers in the EpiTect Methyl qPCR Array designed? FAQ ID -2735 View On which instrumentation will the RT² Profiler PCR Array work? FAQ ID -2719 View Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays? FAQ ID -2747 View What are the advantages of using the SureSilencing shRNA Plasmids over siRNA? FAQ ID -2786 View The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions? FAQ ID -2750 View The pathway reporter luciferase activity values are greater than the positive control, is there a problem? FAQ ID -2766 View The pathway reporter luciferase activity values are less than the negative control, is there a problem? FAQ ID -2767 View How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 View How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 View How can RNA interference be used as a life science research tool? FAQ ID -2756 View How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 View How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 View How can I normalize my EpiTect ChIP results? FAQ ID -2751 View What is the recommended amount of input template for each RT² qPCR Primer Assay? FAQ ID -2716 View What is the RT² Profiler PCR Array? FAQ ID -2718 View Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 View Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 View Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 View Can I manually set the threshold line? FAQ ID -2702 View Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 View Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 View Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 View Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 View Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 View At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 View Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 View Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 View Can I transform the Cignal Reporter Assays? FAQ ID -2762 View Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 View How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 View How many housekeeping genes are included in each PCR Array? FAQ ID -2704 View Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 View What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 View How reliable are the results from the miScript Primer Assays? FAQ ID -2730 View What are EpiTect ChIP qPCR Assays? FAQ ID -2720 View How do you determine the efficiency using the PCR array? FAQ ID -2701 View How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 View How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 View How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 View How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 View How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 View What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 View Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 View What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 View 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? View 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? View 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? View 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? View 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? View 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? View 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? View 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? View What sample types can be tested on the arrays/assays? FAQ ID — 3399 View What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 View Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 View What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 View View What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 View What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 View 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 View 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 View 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 View 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 View 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 View 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 View 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 View Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 View 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? View 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? View 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? View 3314-What is the concentration of RNase A sold separately? View CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 View 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? View Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 View Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 View When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 View Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 View Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 View What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 View Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 View Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately? FAQ ID - 3389 View What is the composition of elution buffers used in QIAsymphony DNA Investigator kits? FAQ ID - 3387 View Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately? FAQ ID - 3390 View 3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube? FAQ ID - 3323 View View 3340 - Which operating system is compatible with PyroMark IdentiFire Software? View 3325 - What are the catalog numbers for the packing materials for the QIAcube? View 3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube? View 3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)? View 3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit? View 3337 - Which method is used for QIAGEN sequencing service? View 3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing? View 3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups? View 3342 - How is the unmethylated unconverted EpiTect DNA control to be used? FAQ - 3342 View 3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it? FAQ - 3343 View 3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)? View 3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes? View 3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit? View 3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit? View 3320 - What is the sensitivity of the Type-it HRM PCR Kit? View 3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit? View 3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit? View Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388 View Do you have data to show lowest dropout rate with Repli-G WTA Single Cells kit? FAQ ID - 3392 View 3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits? 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