Product FAQ

 

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Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
Can I select FITC labeled cells with BioMag?
FAQ ID -278
Can I scale up reagents if I want to use more sample with the MagAttract HMW DNA Kit (cat. no. 67563)?
FAQ ID - 3448
Can I run RUO protocols on the new or upgraded BioRobot EZ1 DSP?
FAQ ID -1563
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology?
FAQ ID -2738
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?

FAQ ID - 3473

Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?
FAQ ID -2896
Can I re-amplify whole genome amplification product?
FAQ ID -670
Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
Can I program my own protocols for the QIAcube?
FAQ ID -1405
Can I program my own protocols for the QIAcube Connect?
FAQ ID - 141486
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
Can I order the nucleotides from PyroMark Gold Reagents separately?
FAQ ID -2827
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased?
FAQ ID -2709
Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
Can I measure virulence factor gene expression?
FAQ ID — 3405
Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
Can I manually set the threshold line?
FAQ ID -2702
Can I make stable cell lines with the Cignal Reporter Assays?
FAQ ID -2763
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?
FAQ ID -1146
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
Can I get IQ/OQ documents from QIAGEN so that I can perform my own IQ/OQ?
FAQ ID -1558
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
Can I directly transfect SureSilencing shRNA Plasmids into my cells?
FAQ ID -2899
Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
Can I change the language on my QIAsymphony SP system?
FAQ ID - 3595
Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?
FAQ ID - 3388
Can I buy the gDNA eliminator plates supplied in your RNeasy Plus 96 kit separately?
FAQ ID - 3389
Can I buy the gDNA eliminator columns supplied in your RNeasy Plus kits separately?
FAQ ID - 3390
Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?
FAQ ID - 3726
Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?
FAQ ID - 3724
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
Can I add bleach to the sample preparation waste?
FAQ ID - 3505
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
Can Glutathione Superflow resin be reused?
FAQ ID -2171
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Can Dithiothreitol (DTT) be used instead of BME in Buffer RLT Plus of RNeasy Plus Mini Kit?
FAQ ID -3125
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
Can cDNA synthesized with the miScript Plant RT Kit be used in real-time PCR with TaqMan® MicroRNA Assays?
FAQ ID - 3445
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Can blood samples be frozen in PAXgene Blood DNA Tubes (IVD)?
FAQ ID - 3377

 

Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
Can BioMag be used for positive and negative selection?
FAQ ID -269
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
Can additional positive controls be used for the Investigator IDplex GO! Kit?
FAQ ID -3094
Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?
FAQ ID -1164
Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?
FAQ ID -1828
Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it?
FAQ ID - 3562
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
At what temperature should I store the Investigator Quantiplex Pro RGQ Kit?
FAQ ID - 3748
At what temperature should I store the Investigator Quantiplex Pro Kit?
FAQ ID - 3737
At what temperature should I store the Investigator Quantiplex Kit?
FAQ ID -2578
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
Aside from the OTV test, what other maintenance should be done for the Rotor-Gene Q?
FAQ ID -3181
As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
Are there stopping points in the Hi-C workflow?
FAQ ID - 143071
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
Are there special requirements for flow sorting?
FAQ ID - 3546
Are there special requirements for flow sorting?
FAQ ID - 3558
Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3635
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
Are there important considerations for plasma generation and urine handling?
FAQ ID - 3699
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
Are there any unique elements that need to be incorporated into an shRNA expression vector?
FAQ ID -2772
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes (IVD) (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3371
Are there any general recommendations to take into account when performing a nucleic acid extraction?
FAQ ID -1494
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Are the QIAshredder columns included in various QIAGEN kits the same?
FAQ ID - 3624
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
Are the primer sets different for the Investigator Nonaplex ESS Kit and Investigator Hexaplex ESS Kit where the loci are the same?
FAQ ID -2400
Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
FAQ ID -2399
Are the primer sets contained in the Investigator IDplex kit the same as in competitor kits?
FAQ ID -2407
Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
Are the miRNA Target Protector & miRNA mimic bench tested? Any guarantee?
FAQ ID - 3186
Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
Are the assays species-specific?
FAQ ID — 3400
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3614
Are scoring sheets available for each crystallization condition Screening Suite?
FAQ ID -1125
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
Are RNeasy spin columns sold separately?
FAQ ID -159
Are real-time PCR results obtained with artus® kits quantifiable and how many standards are required?
FAQ ID -1388
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
Are QR codes recognized by QIAsymphony?
FAQ ID - 3625
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of?
FAQ ID - 3369
Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1196
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
Are plant miScript Primer Assay designs wet-bench validated?
FAQ ID - 3440
Are peak heights of Q48 comparable to other PyroMark instruments?
FAQ ID - 3692
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Are PAXgene Blood DNA Tubes (IVD) sterile?
FAQ ID - 3373
Are PAXgene Blood ccfDNA Tubes sterile?
FAQ ID - 3636
Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID -2398
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
Are miScript primer assays bench-validated?
FAQ ID -3145
Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
Are miScript Primer Assay included in miScript miRNA PCR Arrays the same primers as the miScript Primer Assays available in individual tubes?
FAQ ID - 3441

 

Are MaXtract tubes siliconized?
FAQ ID -1298
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?
FAQ ID - 3609
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?
FAQ ID -482
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples?
FAQ ID -2733
Are EasyXtal 15-Well Tools stackable?
FAQ ID -1116
Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?
FAQ ID -786
Are BioMag particles stable in other solvents?
FAQ ID -267
Are artus® kits legally authorized?
FAQ ID -1372
Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components?
FAQ ID - 3569
Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components?
FAQ ID - 3436
Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?
FAQ ID - 3662
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
A white precipitate has formed in my 10x RT buffer. Is it still ok to use?
FAQ ID -216
895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol?
FAQ - 3345
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
3340 - Which operating system is compatible with PyroMark IdentiFire Software?
3339 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) negatively impact subsequent NGS sequencing?
3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
3337 - Which method is used for QIAGEN sequencing service?
3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
3325 - What are the catalog numbers for the packing materials for the QIAcube?
3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?

 

3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323

 

3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
3320 - What is the sensitivity of the Type-it HRM PCR Kit?
3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
3314-What is the concentration of RNase A sold separately?
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
3302 - How long can I store my purified RNA?
3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
3298-Will the sequence of the QuantiFast Probe Assays be provided?
3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
3292-How do I start using the NeXtal CubicPhase kits?
3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
3285-How are the genes in each iPSC qBiomarker PCR Array selected?
3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
3272 - Are the QuantiFast Probe Assays wet-lab validated?
3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
3261 - Which purification kits are recommended for the therascreen Pyro kits?
3260 - Which purification kits are recommended for the Pyro kits?
3259 - Which probe labels are available for the QuantiFast Probe Assays?
3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
3253 - What species are QuantiTect Probe Assays offered for?
3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
3248 - What is the difference between sponge, cubic and lamellar phase?
3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
3244 -What is the average length of a QuantiFast Probe Assay?
3243 - What does the Investigator Nonaplex ESS Kit contain?
3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
3225 - How pure should my protein be for the NexTal CubicPhase kits?
3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
3217 - How long can I store my purified RNA?
3215 - What is the starting material for SBTexcellerator HLA Kits?
3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
3199 - Which species can be used with the GeneRead rRNA depletion kit?
3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
3197 -Where can I find the Population Data for the Investigator HDplex kit?
3196 - What is the DIPsorter Freeware?
3195 - Where can I find the Population Data for the Investigator DIPlex kit?
3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?
3193 - Where can I get an RNase-free syringe that is recommended for reconstituting QIAGEN's RNase-free DNase (cat. no. 79254)?
3192 - Is QIAGEN Protease compatible with Buffer ATL?
3191 - Is the Allprep DNA Mini Spin Column the same as the gDNA eliminator column? Can I interchange these columns?
3190 - What is the smallest elution volume for RNeasy Mini Spin column?
3188 - How many His residues are required for successful purification on Ni-NTA resins?
3187 - Why does the Ni-NTA resin turn reddish-brown after mixing with the lysates?
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
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