Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays? FAQ ID -2430 View How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay? FAQ ID -2431 View How do I get started using the NeXtal CubicPhase kits? FAQ ID -2413 View After bisulfite conversion, can the DNA be stored? FAQ ID -2409 View Can I buy QIAquick and MinElute columns separately? FAQ ID -2460 View How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit? FAQ ID -2497 View How pure should my protein be with the NexTal CubicPhase kits? FAQ ID -2414 View How many samples can be processed with the PAXgene Blood RNA MDx Kit? FAQ ID -2490 View How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate? FAQ ID -2485 View In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page? FAQ ID -2455 View If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit? FAQ ID -2492 View How do I determine if my gene expression results are statistically significant in the REST software? FAQ ID -2458 View How does the DNA Protection work in the EpitTect Bisulfite kits? FAQ ID -2410 View How is the hypothesis test performed in the REST software? FAQ ID -2459 View How many different genes and gene mutations are on a Somatic Mutation PCR Array? FAQ ID -2427 View How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q? FAQ ID -2452 View How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am? FAQ ID -2421 View How do I harvest the crystals from meso phase? Are special tools needed? FAQ ID -2422 View How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit? FAQ ID -2495 View What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit? FAQ ID -2359 View Will the sequence of the QuantiFast Probe Assays be provided? FAQ ID -2369 View Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)? FAQ ID -2366 View Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit? FAQ ID -2345 View Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA? FAQ ID -2365 View What dedicated QIAcube Kits are available? FAQ ID -2337 View What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components? FAQ ID -2355 View What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays? FAQ ID -2438 View What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit? FAQ ID -2358 View What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays? FAQ ID -2370 View What is the average length of a QuantiFast Probe Assay? FAQ ID -2374 View Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues? FAQ ID -2343 View Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products? FAQ ID -2331 View Is the T790M mutation included in the EGFR PCR kit? FAQ ID -2314 View Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers? FAQ ID -2373 View Is there also a miRNeasy Plus Universal Kit available? FAQ ID -2346 View I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit? FAQ ID -2342 View I would like to order the QuantiFast Probe Assays without master mix. Is this possible? FAQ ID -2360 View My ADAPT report states "Run Invalid" and does not display any sample results. Why is this? FAQ ID -2306 View Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods. FAQ ID -2349 View Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit? FAQ ID -2382 View Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit? FAQ ID -2315 View Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection? FAQ ID -2320 View Which probe labels are available for the QuantiFast Probe Assays? FAQ ID -2361 View Which purification kits are recommended for the Pyro kits? FAQ ID -2380 View What should be the concentration of my protein with the NexTal Cubic Phase products? FAQ ID -2325 View What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit? FAQ ID -2316 View What plug-ins are available for the Pyrosequencing kits? FAQ ID -2381 View Which crystallization solutions are compatible with lipidic cubic phase crystallization? FAQ ID -2418 View Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2352 View What species are QuantiTect Probe Assays offered for? FAQ ID -2367 View Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2348 View Can the insertions in Exon 20 be detected with the EGFR Pyro Kit? FAQ ID -2383 View Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA? FAQ ID -2364 View Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids? FAQ ID -2317 View Can I use the RNeasy Plus Universal Mini Kit for all types of tissue? FAQ ID -2338 View Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits? FAQ ID -2300 View Can QuantiFast Probe Assays be ordered offline via e-mail or fax? FAQ ID -2368 View For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used? FAQ ID -2319 View For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used? FAQ ID -2339 View Does the KRAS PCR kit cover the codon 61 mutation? FAQ ID -2308 View Do you have any information or guidelines regarding the choice of reference genes for real-time PCR? FAQ ID -2371 View Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors? FAQ ID -2318 View Are the QuantiFast Probe Assays hydrolysis or FRET probes? FAQ ID -2363 View Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles? FAQ ID -2334 View Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit? FAQ ID -2333 View Can I use more than 10ng DNA for the PCR reaction for the Pyro kits? FAQ ID -2379 View Can I do singleplex PCR with the QuantiFast Probe Assays? FAQ ID -2362 View Are the QuantiFast Probe Assays wet-lab validated? FAQ ID -2375 View Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits? FAQ ID -2301 View At what temperature can I perform the experiment with the NexTal Cubic Phase kits? FAQ ID -2323 View How much can the mixed standard ?Ct values vary in the KRAS PCR kit? FAQ ID -2307 View How many samples can be processed per RNeasy Mini QIAcube kit? FAQ ID -2332 View How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2353 View How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate? FAQ ID -2357 View How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be? FAQ ID -2344 View How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit? FAQ ID -2351 View How much time can be saved when using the RNeasy Mini QIAcube Kit? FAQ ID -2335 View How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work? FAQ ID -2341 View How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination? FAQ ID -2354 View How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered? FAQ ID -2440 View How are the genes in each iPSC qBiomarker PCR Array selected? FAQ ID -2439 View How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix? FAQ ID -2356 View How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit? FAQ ID -2350 View How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit? FAQ ID -2340 View How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products? FAQ ID -2330 View Is a special processing protocol needed? FAQ ID -2523 View What is the detection limit for the Investigator Quantiplex Kit? FAQ ID -2572 View Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks? FAQ ID -2524 View The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest? FAQ ID -2555 View What are the dimensions of the TissueLyser LT? FAQ ID -2545 View On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used? FAQ ID -2596 View What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -2534 View What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction? FAQ ID -2570 View What is in the elution buffer in the PAXgene Bone Marrow RNA kit? FAQ ID -2511 View What are the storage conditions for the mericon Assay kits? FAQ ID -2540 View Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C? -20 View Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing? FAQ ID -2525 View Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues? FAQ ID -2531 View Is the morphology after H&E staining comparable to formalin-fixed samples? FAQ ID -2526 View Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube? FAQ ID -2509 View Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System? FAQ ID -2514 View Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex? FAQ ID -2568 View What is the maximum tissue size that can be fixed in a PAXgene Tissue Container? FAQ ID -2518 View Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1? FAQ ID -2575 View What RNA yields are expected with the PAXgene Bone Marrow RNA System? FAQ ID -2506 View What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID -2538 View What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits? FAQ ID -2543 View What is the expected amplicon size when using the mericon Assay kits? FAQ ID -2544 View What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use? FAQ ID -2560 View What is the typical amount of tissue used for disruption with the TissueLyser LT? FAQ ID -2552 View What kind of sample tubes are recommended to use with the TissueLyser LT? FAQ ID -2553 View What is the purity of nucleic acids extracted with the PAXgene Tissue kits? FAQ ID -2533 View Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA? FAQ ID -2507 View Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples? FAQ ID -2501 View Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach? FAQ ID -2515 View Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS? FAQ ID -2530 View Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin? FAQ ID -2502 View Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen? FAQ ID -2549 View Can the TissueLyser LT Adapter be removed for cleaning? FAQ ID -2546 View Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes? FAQ ID -2595 View Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins? FAQ ID -2548 View When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent? FAQ ID -2556 View When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen? FAQ ID -2594 View Which human target is used for the quantification in the Investigator Quantiplex Kit? FAQ ID -2577 View What sample numbers can be processed on the TissueLyser LT? FAQ ID -2547 View What size bead should I use for tougher samples with the TissueLyser LT? FAQ ID -2554 View When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX? FAQ ID -2597 View Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low? FAQ ID -2574 View Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex? FAQ ID -2569 View Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation? FAQ ID -2508 View Are there any accessories required for sample disruption with the TissueLyser LT? FAQ ID -2551 View At what temperature should I store the Investigator Quantiplex Kit? FAQ ID -2578 View Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube? FAQ ID -2503 View Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target? FAQ ID -2598 View Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit? FAQ ID -2512 View Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System? FAQ ID -2510 View Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization? FAQ ID -2529 View How long is the fixation time? FAQ ID -2519 View Can HiPerFect HTS be used for miRNA? FAQ ID -2561 View Can HiPerFect HTS Reagent be used for transfecting shRNAs? FAQ ID -2559 View How long does the sample disruption with the TissueLyser LT take? FAQ ID -2550 View How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed? FAQ ID -2599 View How long can I store the dilutions of the Control DNA Z1 standards at 2°C? -8 View How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted? -20 View How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long? FAQ ID -2505 View How long does an Investigator Quantiplex run take? FAQ ID -2566 View How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube? FAQ ID -2504 View I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation? FAQ ID -2513 View Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System? FAQ ID -2571 View Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA? FAQ ID -2557 View How can I streamline the quantification workflow for the Investigator QuantiPlex Kit? FAQ ID -2579 View How is stability and performance of these mericon Assay kits guaranteed? FAQ ID -2542 View How long are the target sequences of Investigator Quantiplex? FAQ ID -2576 View For which real-time cyclers has the Investigator Quantiplex Kit been validated? FAQ ID -2567 View How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed? FAQ ID -2516 View View What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced? FAQ ID -3055 View Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings? FAQ ID -3054 View Why are two reagents used in the PAXgene Tissue System? FAQ ID -3022 View Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit? FAQ ID -3095 View Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay? FAQ ID -3092 View Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples? FAQ ID -3091 View Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit? FAQ ID -3090 View Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers? FAQ ID -3089 View What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit? FAQ ID -3096 View Can additional positive controls be used for the Investigator IDplex GO! Kit? FAQ ID -3094 View Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit? FAQ ID -3088 View Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit? FAQ ID -3093 View What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)? FAQ ID -3024 View 5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container? FAQ ID -3023 View What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)? FAQ ID -3025 View Is it possible to fix over a weekend? FAQ ID -3027 View How long is the fixation time when using PAXgene Tissue FIX Container? FAQ ID -3026 View How long can a tissue specimen stay in the PAXgene Tissue STABILIZER? FAQ ID -3028 View What is the minimum incubation time required when using the PAXgene Tissue STABILIZER? FAQ ID -3029 View Is it possible to integrate the PAXgene Tissue STABILIZER into the processing? FAQ ID -3037 View Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3038 View Is the morphology after H&E staining comparable to formalin-fixed samples? FAQ ID -3039 View Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3040 View Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3043 View is the purity of nucleic acids isolated with the PAXgene Tissue Kits? FAQ ID -3044 View What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3045 View What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3046 View What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID -3050 View How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed? FAQ ID -3052 View What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue? FAQ ID -3051 View What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3047 View Does the PAXgene Tissue RNA Kit co-purify small RNAs? FAQ ID -3048 View How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue? FAQ ID -3049 View Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization? FAQ ID -3042 View What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)? FAQ ID -3086 View What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols? FAQ ID -3083 View What are the typical yields obtained from Buccal swabs on BioSprint 96? FAQ ID -3071 View Where can I find a copy of the .kpf file for a BioSprint protocol? FAQ ID -3073 View Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730? FAQ ID -3075 View Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C? FAQ ID -3079 View Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification? FAQ ID -3080 View What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility? FAQ ID -3064 View How precise is the QIAgility? FAQ ID -3063 View How long does it take to set up a 96-well plate PCR reaction on the QIAgility? FAQ ID -3066 View Can I use a bar code scanner or reader on the QIAgility? FAQ ID -3067 View Can I use a USB drive to transfer protocols to BioSprint? FAQ ID -3072 View Can BioSprint 96 be connected to LIMS? FAQ ID -3070 View How can I find the run parameters for a BioSprint protocol that I am running? FAQ ID -3069 View Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored? FAQ ID -3077 View Can I use specific primers with the FastLane Kits? FAQ ID -3078 View What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits? FAQ ID -3082 View What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit? FAQ ID -3081 View What is the concentration of the different Proteinase K solutions sold by QIAGEN? FAQ ID -3084 View Is the Fast Reaction Mix ready to use in the Investigator IDplex GO? FAQ ID -3087 View Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit? FAQ ID -3085 View Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples? FAQ ID -3032 View Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures? FAQ ID -3030 View Is it possible to manually process PAXgene Tissue treated samples without using an automated processor? FAQ ID -3036 View Is a special processing protocol needed? FAQ ID -3035 View Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run? FAQ ID -3033 View Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue? FAQ ID -3034 View Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS? FAQ ID -3041 View When should the N2 bottle used with the QIAxcel Advanced System be changed? FAQ ID -3001 View Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same? FAQ ID -3000 View How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit? FAQ ID -3003 View How do I clean up RNA preparations containing miRNA? FAQ ID -3002 View What is the thermal output for a BioRobot MDx and BioRobot Universal? FAQ ID -3056 View What is the thermal output for a QIAsymphony SP and AS instrument? FAQ ID -3057 View Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits? FAQ ID -3053 View What is the minimum number of samples that can be extracted on a BioSprint 96? FAQ ID -3076 View What is the fixation method used in the PAXgene Tissue System? FAQ ID -3019 View What does PAXgene Tissue FIX contain? FAQ ID -3020 View Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells? FAQ ID -3098 View What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit? FAQ ID -1357 View Can the MaXtract High Density Tube be used with QIAzol to isolate RNA? FAQ ID -1304 View I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set? FAQ ID -1366 View What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes? FAQ ID -1312 View If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use? FAQ ID -1310 View How will service work carried out on my QIAGEN BioRobot be documented? FAQ ID -1331 View If I cannot order FlexiPlate siRNA online, how do I order? FAQ ID -1371 View What information about my gene or siRNA do I need to order FlexiPlate siRNA? FAQ ID -1365 View Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)? -3 View What information is provided with FlexiPlate siRNAs? FAQ ID -1363 View Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water? FAQ ID -1355 View Which formats are available for FlexiPlate siRNA? FAQ ID -1358 View Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot? FAQ ID -1334 View When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used? FAQ ID -1308 View Which controls can I choose for FlexiPlate siRNA? FAQ ID -1368 View Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA? FAQ ID -1328 View Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure? FAQ ID -1327 View Which density gel type is contained in the yellow or green MaXtract Tubes? FAQ ID -1315 View What is the composition of FlexiPlate siRNA buffer? FAQ ID -1361 View Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument? FAQ ID -1336 View What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit? FAQ ID -1356 View Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit? FAQ ID -1325 View Can MaXtract tubes be autoclaved prior to use? FAQ ID -1305 View Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit? FAQ ID -1326 View Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit? FAQ ID -1321 View Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time? FAQ ID -1352 View Can several extraction steps be performed in the same MaXtract tube? FAQ ID -1302 View Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density? FAQ ID -1309 View Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing? FAQ ID -1303 View Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit? FAQ ID -1319 View Can I order Alexa Fluor labeled controls with FlexiPlate siRNA? FAQ ID -1369 View Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes? FAQ ID -1301 View Can HP Validated siRNAs be ordered on the FlexiPlate? FAQ ID -1367 View Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit? FAQ ID -1337 View How do I resuspend my FlexiPlate siRNAs? FAQ ID -1360 View How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction? FAQ ID -1320 View Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit? FAQ ID -1323 View Do you have stability data for genomic DNA isolated with the BioSprint Kits? FAQ ID -1318 View Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence? FAQ ID -1307 View Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit? FAQ ID -1317 View How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot? FAQ ID -1330 View How can I upload my gene list for FlexiPlate siRNA to GeneGlobe? FAQ ID -1364 View How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit? FAQ ID -1354 View How can I ensure optimal performance of my QIAGEN instrument? FAQ ID -1329 View What is the "seed sequence" of a miRNA? FAQ ID -2262 View What is the turn around time for the ordering of miScript Target Protector? FAQ ID -2254 View What is the sample throughput of Pyrosequencing systems? FAQ ID -2215 View What is the processing time for 96 normalizations with the SeqTarget System? FAQ ID -2237 View What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System? FAQ ID -2247 View What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit? FAQ ID -2292 View What technologies are used by the KRAS and EGFR PCR kits? FAQ ID -2286 View What kind of automation can be done with the EasyXtal 15-well Tool? FAQ ID -2220 View What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis? FAQ ID -2216 View What dyes are used in the KRAS and EGFR PCR kits? FAQ ID -2288 View What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits? FAQ ID -2298 View What format are the EasyXtal 15-well tools? FAQ ID -2219 View What do I need to provide when ordering a miScript Target Protector? FAQ ID -2251 View What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector? FAQ ID -2253 View What is a miScript Precursor Assay? FAQ ID -2214 View What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2203 View What is a miScript Target Protector? FAQ ID -2249 View Which transfection reagent is recommended for transfection of the miScript Target Protectors? FAQ ID -2252 View Which variants of the EasyXtal 15-well tool are available? FAQ ID -2221 View Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software? FAQ ID -2205 View Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software? FAQ ID -2204 View Why does the SeqTarget Prep Protocol require a 2-step procedure? FAQ ID -2238 View Why does the miScript Target Protector not induce the degradation process? FAQ ID -2263 View Why do I have wavy DNA bands on my agarose gel? FAQ ID -2260 View What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2? FAQ ID -2242 View When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them? FAQ ID -2294 View What type of samples can I use for the KRAS PCR and EGFR kits? FAQ ID -2293 View What voltage should be used to run my agarose gel with the GelPilot Agarose? FAQ ID -2261 View Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised? FAQ ID -2202 View Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance? FAQ ID -2267 View Does the miScript Target Protector match the target sequence? FAQ ID -2250 View Do the KRAS and EGFR PCR kits contain a passive reference dye? FAQ ID -2295 View Do I have to order at least 4 FlexiTube siRNA Premixes? FAQ ID -2266 View How do I store my FlexiTube siRNA Premix? FAQ ID -2270 View How is ARMS technology superior to alternative methods such as dye terminator sequencing? FAQ ID -2290 View Effects of low A260/A230 ratios in RNA preparations on downstream applications FAQ ID -2248 View How can I check the success of the normalization procedure for the SeqTarget System? FAQ ID -2245 View How do I search for all the miScript Target Protectors for one species? FAQ ID -2255 View Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick? FAQ ID -2240 View Can PCR fragments be normalized for the SeqTarget System without a previous purification step? FAQ ID -2246 View Can I use a 384 well plate for the KRAS and EGFR PCR kits? FAQ ID -2296 View Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used? FAQ ID -2234 View Can the miScript Precursor Assay differentiate between pri- and pre-miRNA? FAQ ID -2211 View Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System? FAQ ID -2244 View What are EasyXtal 15-well Tools used for? FAQ ID -2217 View What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software? FAQ ID -2200 View Is the SeqTarget System compatible with standard-size PCRs? FAQ ID -2241 View What are the amplicon lengths of the KRAS and EGFR PCR kits? FAQ ID -2289 View What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2201 View What does QIAGEN use for a positive control in precursor detection experiments? FAQ ID -2212 View What controls are used in the KRAS and EGFR PCR kits to ensure reliable results? FAQ ID -2291 View What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits? FAQ ID -2297 View What are the corresponding QIAGEN names for former Biotage instruments? FAQ ID -2285 View What buffer conditions give the best resolution for agarose gel electrophoresis? FAQ ID -2256 View What can be the reason for low DNA yields after normalization with the SeqTarget System? FAQ ID -2239 View How much buffer should be used for agarose gel electrophoresis? FAQ ID -2257 View How much DNA should be loaded per well of an agarose gel? FAQ ID -2259 View How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved? FAQ ID -2258 View How long does it take to generate results using the KRAS and EGFR PCR kits? FAQ ID -2299 View How long should PCR fragments be for the SeqTarget System? FAQ ID -2236 View How many miScript Precursor Assays do you offer? FAQ ID -2213 View Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix? FAQ ID -2264 View Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types? FAQ ID -2268 View Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types? FAQ ID -2269 View If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do? FAQ ID -2265 View Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed? FAQ ID -2235 View Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System? FAQ ID -2243 View Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits? FAQ ID -1484 View Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits? FAQ ID -1451 View What applications are offered on the QIAcube? FAQ ID -1403 View Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits? FAQ ID -1449 View Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits? FAQ ID -1446 View Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits? FAQ ID -1447 View Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers? FAQ ID -1442 View What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates? FAQ ID -1468 View Can purification columns from other suppliers be processed on the QIAcube? FAQ ID -1406 View Can QIAcube protocol runs be interrupted, and subsequently be continued to completion? FAQ ID -1414 View Are run reports and/or log files available on the QIAcube? FAQ ID -1412 View Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR? FAQ ID -1480 View Are FlexiPlate siRNAs available at a higher final concentration than 10 µM? FAQ ID -1476 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits? FAQ ID -1452 View Can different amounts of siRNA be ordered on one FlexiPlate? FAQ ID -1473 View Can 2 µl reaction volumes be used with QuantiFast PCR Kits? FAQ ID -1440 View Are there any specific cleaning solutions recommended for the QIAcube? FAQ ID -1416 View Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits? FAQ ID -1439 View Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex? FAQ ID -1437 View Do I need to buy special kits for the QIAcube? FAQ ID -1402 View Do I need to discard partially used QIAcube tip racks? FAQ ID -1418 View What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format? FAQ ID -1463 View What are the turnaround times for FlexiPlate siRNA orders? FAQ ID -1478 View What are the dimensions and weight of the QIAcube? FAQ ID -1407 View How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits? FAQ ID -1438 View How many reactions can I perform with the new QuantiFast Kits for real-time PCR? FAQ ID -1425 View If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs? FAQ ID -1477 View How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling? FAQ ID -1430 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? FAQ ID -1415 View Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits? FAQ ID -1450 View Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration? FAQ ID -1443 View What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex? FAQ ID -1436 View What is the detection limit of the QuantiFast Kits for real-time PCR? FAQ ID -1432 View Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs? FAQ ID -1459 View Which cell lines have been tested with FastLane Cell RT-PCR Kits? FAQ ID -1485 View Can I adjust the ROX concentration in the QuantiFast master mix? FAQ ID -1427 View Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates? FAQ ID -1458 View Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates? FAQ ID -1482 View Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes? FAQ ID -1433 View Can FlexiPlate siRNA be ordered in solution on dry-ice? FAQ ID -1474 View Can I program my own protocols for the QIAcube? FAQ ID -1405 View How can I load new protocols onto the QIAcube? FAQ ID -1421 View How can I get software updates for the QIAcube? FAQ ID -1413 View How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR? FAQ ID -1441 View Do you offer trial-kit sizes for the new QuantiFast Kits? FAQ ID -1429 View How can I decontaminate the QIAcube system? FAQ ID -1419 View Can the QIAcube heater/shaker be used independently from protocol runs? FAQ ID -1422 View Can the QIAcube rotor adapters be autoclaved? FAQ ID -1409 View Can the QIAcube centrifuge be used independently from protocol runs? FAQ ID -1423 View Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options? FAQ ID -1428 View Can the QIAcube be connected to a laboratory information management system? FAQ ID -1411 View Can the QIAcube rotor and/or buckets be removed for cleaning? FAQ ID -1410 View Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity? FAQ ID -1444 View Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments? FAQ ID -1431 View Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye? FAQ ID -1435 View What are the Tms of PCR products obtained using the miScript System? FAQ ID -1599 View Do you have a protocol for cleanup of REPLI-g amplified DNA? FAQ ID -1545 View What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1580 View What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System? FAQ ID -1596 View What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits? FAQ ID -1554 View Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits? FAQ ID -1553 View Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent? FAQ ID -1572 View Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol? -8 View Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification? FAQ ID -1573 View Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis? FAQ ID -1587 View Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit? FAQ ID -1547 View Do you have a protocol for concentration of RNA in 96-well plates? FAQ ID -1546 View Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density? FAQ ID -1550 View Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit? FAQ ID -1592 View What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure? FAQ ID -1584 View What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit? FAQ ID -1591 View Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues? FAQ ID -1579 View What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System? FAQ ID -1597 View Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein? FAQ ID -1575 View Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers? FAQ ID -1555 View Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1583 View Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone? FAQ ID -1576 View Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1577 View Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts? FAQ ID -1585 View Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies? FAQ ID -1571 View Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1590 View Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants? FAQ ID -1574 View Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1586 View How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System? FAQ ID -1598 View How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument? FAQ ID -1519 View What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies? FAQ ID -2010 View What is the advantage of using the Type-it Microsatellite PCR Kit? FAQ ID -2059 View What is the advantage of using the Type-it Mutation Detect PCR Kit? FAQ ID -2064 View What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit? FAQ ID -2005 View What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit? FAQ ID -2029 View What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K? -16 View What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP? FAQ ID -2028 View What is the principle behind the DNA Polymerase in the EpiTect MSP Kit? FAQ ID -2003 View Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit? FAQ ID -2072 View What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007 View Is magnetic particle carry-over in the eluates on the BioSprint 96 normal? FAQ ID -2073 View What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template? FAQ ID -2009 View Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP? FAQ ID -2031 View What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA? FAQ ID -2011 View What are the features of the expression vector used for QIAgenes Insect/Mammalia? FAQ ID -2050 View Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)? FAQ ID -2035 View Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit? FAQ ID -2068 View Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 View Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon? FAQ ID -2043 View Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs? FAQ ID -2067 View Will the optimized DNA sequence for QIAgenes be provided? FAQ ID -2045 View Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp? FAQ ID -2060 View Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping? FAQ ID -2057 View What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits? FAQ ID -2037 View What sample types can be processed using the QIAsymphony Virus/Bacteria protocols? FAQ ID -2036 View Can data be analyzed on the Rotor-Gene Q while a run is in progress? FAQ ID -2091 View Are there two vectors for protein expression using QIAgenes in insect or mammalian cells? FAQ ID -2048 View Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit? FAQ ID -2066 View Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit? FAQ ID -2058 View Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP? FAQ ID -2030 View Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results? FAQ ID -2079 View Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run? FAQ ID -2092 View Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q? FAQ ID -2093 View What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit? FAQ ID -2004 View What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit? FAQ ID -2034 View Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs? FAQ ID -2049 View Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol? FAQ ID -2038 View What types of reaction vessels are required for use in the Rotor-Gene Q? FAQ ID -2085 View Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits? FAQ ID -2063 View Can the EpiTect PCR Control DNAs be used for mouse and rat assays? FAQ ID -2008 View Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification? FAQ ID -2006 View Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples? FAQ ID -2001 View Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument? FAQ ID -2027 View Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models? -15 View Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments? FAQ ID -2074 View Do all QIAsymphony DNA Investigator protocols use carrier RNA? FAQ ID -2033 View Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months? FAQ ID -2052 View Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp? FAQ ID -2065 View Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates? FAQ ID -2062 View Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled? FAQ ID -2082 View How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q? FAQ ID -2083 View Can membrane proteins be expressed with QIAgenes expression kits? FAQ ID -2047 View Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure? FAQ ID -2000 View Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run? FAQ ID -2040 View Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument? FAQ ID -2039 View Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q? FAQ ID -2081 View How can a C-terminal 6xHis-tagged QIAgene be expressed? FAQ ID -2041 View How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned? FAQ ID -2051 View Does the Rotor-Gene Q software have the capacity to export data? FAQ ID -2089 View Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL? FAQ ID -2032 View Can frozen samples be used with the MagAttract 96 cador Pathogen Kit? FAQ ID -2070 View How fast does the rotor spin during a run on the Rotor-Gene Q? FAQ ID -2080 View How is the log graph threshold value (Ct) for the Rotor-Gene Q determined? FAQ ID -2088 View How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit? FAQ ID -2002 View How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24? FAQ ID -2095 View How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware? FAQ ID -2071 View If orders cannot be placed online, how can QIAgenes be ordered? FAQ ID -2046 View If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes? FAQ ID -2087 View How long does a single run on the PyroMark Q24 take? FAQ ID -2096 View How much space does the PyroMark Q24 instrument need? FAQ ID -2098 View Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used? FAQ ID -2061 View How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094 View Is there a charge for user training on the QIAsymphony SP System? FAQ ID -1900 View Is the QIAsymphony RNA Kit compatible with stabilized samples? FAQ ID -1944 View Is sample pretreatment required for purifying RNA on the QIAsymphony SP? FAQ ID -1938 View Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols? FAQ ID -1920 View What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -1919 View QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean? FAQ ID -1982 View What are the dimensions and weight of the QIAsymphony SP System? FAQ ID -1901 View What is the range of elution volumes on the QIAsymphony SP? FAQ ID -1908 View How many PCR reactions can be performed for different scales of miScript Primer Assays? FAQ ID -1990 View How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP? FAQ ID -1940 View How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle? FAQ ID -1905 View How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate? FAQ ID -1994 View How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation? FAQ ID -1941 View How much sample can be used with the Allprep DNA/RNA 96 Kit? FAQ ID -1997 View How much sample can be used with the RNeasy Plus 96 Kit? FAQ ID -1992 View How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP? FAQ ID -1917 View What sample types can be used with the AllPrep DNA/RNA 96 Kit? FAQ ID -1996 View Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP? FAQ ID -1937 View What sample types can be used with the RNeasy Plus 96 Kit? FAQ ID -1991 View Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921 View What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP? FAQ ID -1918 View Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus? FAQ ID -1999 View Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit? FAQ ID -1993 View What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays? FAQ ID -1985 View Is it possible to modify QIAsymphony protocols? FAQ ID -1903 View What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP? FAQ ID -1939 View What are the expected yields of total RNA isolated with the QIAsymphony SP? FAQ ID -1942 View What kind of molecules are miScript miRNA Inhibitors? FAQ ID -1983 View What sample input volumes can be used on the QIAsymphony SP? FAQ ID -1907 View What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP? FAQ ID -1915 View What kind of molecules are miScript miRNA Mimics? FAQ ID -1986 View What RNA elution volumes are available with the QIAsymphony RNA Kit? FAQ ID -1943 View Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -1916 View Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits? FAQ ID -1976 View Can uneven sample numbers be processed on the QIAsymphony SP module? FAQ ID -1906 View Does the QIAsymphony SP system generate run reports and/or log files? FAQ ID -1911 View Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors? FAQ ID -1984 View Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits? FAQ ID -1964 View Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit? FAQ ID -1953 View Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits? FAQ ID -1960 View Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits? FAQ ID -1969 View Can I program my own protocols for the QIAsymphony SP? FAQ ID -1904 View Are QuantiFast Multiplex PCR Kits available in trial sizes? FAQ ID -1981 View Are there any specific cleaning solutions recommended for the QIAsymphony SP? FAQ ID -1913 View Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit? FAQ ID -1978 View Can the QIAsymphony RNA Kit be used to purify RNA from blood? FAQ ID -1945 View Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)? FAQ ID -1909 View Can protocol runs on the QIAsymphony SP be interrupted and resumed? FAQ ID -1912 View Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes? FAQ ID -1979 View Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples? FAQ ID -1995 View Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits? FAQ ID -1950 View Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits? FAQ ID -1951 View Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits? FAQ ID -1959 View Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits? FAQ ID -1975 View Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits? FAQ ID -1946 View Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits? FAQ ID -1947 View Do you have a protocol for the purification of DNA from fruit flies? FAQ ID -1970 View Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits? FAQ ID -1974 View How can the QIAsymphony SP system be decontaminated? FAQ ID -1914 View Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits? FAQ ID -1958 View Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits? FAQ ID -1955 View Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits? FAQ ID -1954 View Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits? FAQ ID -1967 View Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits? FAQ ID -1968 View Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits? FAQ ID -1948 View Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits? FAQ ID -1972 View Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits? FAQ ID -1973 View Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits? FAQ ID -1971 View Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits? FAQ ID -1965 View Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits? FAQ ID -1952 View What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits? FAQ ID -1751 View What is the fidelity of TopTaq DNA Polymerase? FAQ ID -1739 View Is TopTaq DNA Polymerase a hot-start polymerase? FAQ ID -1748 View Which nucleotide analogs can be used with TopTaq DNA Polymerase? FAQ ID -1741 View Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit? FAQ ID -1755 View What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits? FAQ ID -1738 View What is the shelf-life of TopTaq DNA Polymerase and Master Mix? FAQ ID -1743 View What is the principle of the REPLI-g FFPE Kit? FAQ ID -1753 View What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit? FAQ ID -1752 View What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit? FAQ ID -1750 View Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit? FAQ ID -1737 View What is the recommended sample size for use with the REPLI-g FFPE Kit? FAQ ID -1756 View Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit? FAQ ID -1749 View Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits? FAQ ID -1702 View Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits? FAQ ID -1740 View At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done? FAQ ID -1758 View Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex? FAQ ID -1714 View How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit? FAQ ID -1734 View Do you have protocols for sample processing on the Autopure LS instrument? FAQ ID -1759 View Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order? FAQ ID -1754 View Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples? FAQ ID -1760 View Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48? FAQ ID -1733 View Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications? FAQ ID -1744 View Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions? FAQ ID -1761 View Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? FAQ ID -1745 View Is FlexiTube GeneSolution available for other scales than the 1 nmol scale? FAQ ID -1666 View What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit? FAQ ID -1601 View What is the binding capacity of the Ni-NTA Superflow Cartridges? FAQ ID -1603 View Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit? FAQ ID -1610 View Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination? FAQ ID -1697 View Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification? FAQ ID -1609 View Is sequence information for HP Validated siRNAs provided in the FlexiTube format? FAQ ID -1665 View Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit? FAQ ID -1614 View Which siRNA controls are available in FlexiTube format? FAQ ID -1661 View Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits? FAQ ID -1690 View Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit? FAQ ID -1620 View What is the composition of Buffer PE? FAQ ID -1691 View What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1616 View Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series? FAQ ID -1606 View Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused? FAQ ID -1607 View Can I store cDNA generated by the miScript System? FAQ ID -1602 View Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples? FAQ ID -1612 View Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants? FAQ ID -1615 View Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase? FAQ ID -1617 View Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time? FAQ ID -1618 View Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs? FAQ ID -1664 View Can I still reorder siRNA that has been removed from the GeneGlobe data base? FAQ ID -1668 View Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields? FAQ ID -1608 View Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution? FAQ ID -1667 View Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube? FAQ ID -1658 View Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits? FAQ ID -1611 View How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644 View How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges? FAQ ID -1605 View How long is cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1613 View How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1623 View Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure? FAQ ID -1619 View Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer? FAQ ID -1659 View Do you have a protocol for purification of total RNA from plant latex? FAQ ID -1694 View Do I need to use the miScript Universal Primer for detection of mRNA? FAQ ID -1600 View Do you offer a QuantiFast Kit for one-step RT-PCR? FAQ ID -1695 View Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit? FAQ ID -1693 View How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol? FAQ ID -2159 View How often can the Strep-Tactin Superflow Plus resin be reused? FAQ ID -2169 View If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit? FAQ ID -2129 View If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software? FAQ ID -2150 View Is it normal that the binding capacity of GST-resins decreases with flow rate? FAQ ID -2174 View Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow? FAQ ID -2172 View Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 View Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits? FAQ ID -2118 View Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122 View Why do I need normalization using Rotor-Gene ScreenClust HRM Software? FAQ ID -2198 View Why is there an asterisk for my miScript Primer Assays? FAQ ID -2176 View Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers? FAQ ID -2121 View Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits? FAQ ID -2139 View Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC? FAQ ID -2124 View What is miRNA*? Do I need to consider this in my profiling experiment? FAQ ID -2179 View What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits? FAQ ID -2119 View What are possible reasons for reduced DNA yields with REPLI-g Kits? FAQ ID -2148 View What is the residuals plot in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2199 View What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2158 View What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates? FAQ ID -2109 View What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits? FAQ ID -2144 View What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit? FAQ ID -2145 View How should DNA isolated on the QIAsymphony be stored? FAQ ID -2165 View How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2157 View How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be? FAQ ID -2193 View Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis? FAQ ID -2188 View Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow? FAQ ID -2173 View Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2160 View What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2156 View Which plate formats are offered for QuantiTect Primer Assay Plates? FAQ ID -2106 View What species are QuantiTect Primer Assay Plates offered for? FAQ ID -2110 View Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits? FAQ ID -2147 View Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2154 View How many reactions can be performed per well of the QuantiTect Primer Assay Plates? FAQ ID -2107 View Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types? FAQ ID -2190 View Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate? FAQ ID -2108 View Can Glutathione Superflow resin be reused? FAQ ID -2171 View Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use? FAQ ID -2138 View Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax? FAQ ID -2112 View Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix? FAQ ID -2168 View Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits? FAQ ID -2134 View Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2130 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2131 View Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers? FAQ ID -2142 View Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit? FAQ ID -2135 View Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis? FAQ ID -2191 View Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument? FAQ ID -2116 View Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)? FAQ ID -2136 View Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online? FAQ ID -2111 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits? FAQ ID -2126 View How does template quality influence the results with the Type-it HRM PCR Kit? FAQ ID -2197 View How does magnetic-particle carry-over in QIAsymphony eluates need to be handled? FAQ ID -2166 View Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits? FAQ ID -2105 View How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments? FAQ ID -2189 View How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits? FAQ ID -2125 View How many elution steps are recommended when using Glutathione Superflow resin? FAQ ID -2175 View How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit? FAQ ID -2192 View Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits? FAQ ID -2104 View Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163 View Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2162 View Can Vapor-Lock be used with samples processed on the QIAgility? FAQ ID -2153 View Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits? FAQ ID -2123 View Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment? FAQ ID -2117 View Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit? FAQ ID -2128 View What main input voltage is required for the QIAxcel Analyzer? FAQ ID -1821 View What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced? FAQ ID -1852 View Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits? FAQ ID -1882 View The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence? FAQ ID -1850 View Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System? FAQ ID -1832 View What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly? FAQ ID -1834 View Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced?? FAQ ID -1847 View Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced? FAQ ID -1844 View Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on? FAQ ID -1851 View Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System? FAQ ID -1828 View Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used? FAQ ID -1841 View Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate? FAQ ID -1861 View How can a positive threshold be set when running the QIAxcel System? FAQ ID -1843 View How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System? FAQ ID -1827 View How long will the intensity calibration procedure on the QIAxcel System take? FAQ ID -1826 View Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed? FAQ ID -1824 View Will the QIAxcel System run using a green Data Review Software Key? FAQ ID -1853 View Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer? FAQ ID -1848 View Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced? FAQ ID -1835 View When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider? FAQ ID -1842 View What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced? FAQ ID -1833 View Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced? FAQ ID -1837 View When should an intensity calibration be re-performed on the QIAxcel System? FAQ ID -1825 View When should the N2 bottle used with the QIAxcel System be changed? FAQ ID -1830 View Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System? FAQ ID -1855 View Can the QIAxcel System or QIAxcel Advanced be connected to a network? FAQ ID -1862 View How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced? FAQ ID -1836 View Can multiple QIAxcel Systems be run from one computer? FAQ ID -1860 View If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated? FAQ ID -1858 View If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created? FAQ ID -1857 View How often should the alignment marker for the QIAxcel System be changed? FAQ ID -1839 View How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced? FAQ ID -1840 View In the results table generated with the QIAxcel System, what does "normalized area" mean? FAQ ID -1854 View How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented? FAQ ID -1823 View If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created? FAQ ID -1856 View How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced? FAQ ID -1838 View What is the composition of buffer QBT? FAQ ID -411 View What is the composition of buffer QC? FAQ ID -412 View What is the composition of buffer P3? FAQ ID -418 View What is the composition of buffer QF? FAQ ID -413 View What is the composition of buffer TE? FAQ ID -416 View What is the composition of buffer QN? FAQ ID -414 View What is the composition of buffer OCD? FAQ ID -423 View What is the composition of buffer FWB2? FAQ ID -417 View What is the composition of buffer OBB? FAQ ID -421 View What is the composition of buffer OW2? FAQ ID -419 View What is the composition of buffer OL2? FAQ ID -424 View What is the composition of buffer OCL? FAQ ID -422 View Can RNAi experiments be performed in Drosophila? FAQ ID -436 View What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors? FAQ ID -470 View Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant? FAQ ID -472 View Has RNAi been successful using siRNA in Zebrafish and Xenopus? FAQ ID -400 View How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit? FAQ ID -468 View How do I calculate the percentage of silencing with real-time RT-PCR for siRNA? FAQ ID -498 View Are Northern Blots sensitive enough to detect siRNA-induced gene silencing? FAQ ID -403 View Can QIAquick Kits be used to clean up RNA samples? FAQ ID -490 View Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit? FAQ ID -482 View Can Ni-NTA resins be used to purify protein with an internal His-tag? FAQ ID -496 View What is the composition of buffer STE? FAQ ID -415 View Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction? FAQ ID -429 View What is the composition of buffer OEB? FAQ ID -420 View Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed? FAQ ID -474 View Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues? FAQ ID -467 View Why is the pQE DNA provided in QIAexpress Kits blue in color? FAQ ID -487 View Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473 View What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit? FAQ ID -432 View What is the minimum elution volume when using the RNeasy Micro Kit? FAQ ID -428 View What is the smallest sample size that can be used with RNeasy Mini Kits? FAQ ID -485 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care

How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?

FAQ ID -2430

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How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?

FAQ ID -2431

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How do I get started using the NeXtal CubicPhase kits?

FAQ ID -2413

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After bisulfite conversion, can the DNA be stored?

FAQ ID -2409

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Can I buy QIAquick and MinElute columns separately?

FAQ ID -2460

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How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?

FAQ ID -2497

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How pure should my protein be with the NexTal CubicPhase kits?

FAQ ID -2414

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How many samples can be processed with the PAXgene Blood RNA MDx Kit?

FAQ ID -2490

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How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?

FAQ ID -2485

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In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?

FAQ ID -2455

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If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?

FAQ ID -2492

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How do I determine if my gene expression results are statistically significant in the REST software?

FAQ ID -2458

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How does the DNA Protection work in the EpitTect Bisulfite kits?

FAQ ID -2410

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How is the hypothesis test performed in the REST software?

FAQ ID -2459

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How many different genes and gene mutations are on a Somatic Mutation PCR Array?

FAQ ID -2427

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How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?

FAQ ID -2452

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How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?

FAQ ID -2421

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How do I harvest the crystals from meso phase? Are special tools needed?

FAQ ID -2422

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How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?

FAQ ID -2495

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What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?

FAQ ID -2359

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Will the sequence of the QuantiFast Probe Assays be provided?

FAQ ID -2369

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Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?

FAQ ID -2366

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Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?

FAQ ID -2345

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Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?

FAQ ID -2365

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What dedicated QIAcube Kits are available?

FAQ ID -2337

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What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?

FAQ ID -2355

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What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?

FAQ ID -2438

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What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?

FAQ ID -2358

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What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?

FAQ ID -2370

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What is the average length of a QuantiFast Probe Assay?

FAQ ID -2374

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Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?

FAQ ID -2343

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Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?

FAQ ID -2331

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Is the T790M mutation included in the EGFR PCR kit?

FAQ ID -2314

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Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?

FAQ ID -2373

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Is there also a miRNeasy Plus Universal Kit available?

FAQ ID -2346

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I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?

FAQ ID -2342

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I would like to order the QuantiFast Probe Assays without master mix. Is this possible?

FAQ ID -2360

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My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?

FAQ ID -2306

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Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.

FAQ ID -2349

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Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?

FAQ ID -2382

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Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?

FAQ ID -2315

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Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?

FAQ ID -2320

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Which probe labels are available for the QuantiFast Probe Assays?

FAQ ID -2361

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Which purification kits are recommended for the Pyro kits?

FAQ ID -2380

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What should be the concentration of my protein with the NexTal Cubic Phase products?

FAQ ID -2325

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What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?

FAQ ID -2316

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What plug-ins are available for the Pyrosequencing kits?

FAQ ID -2381

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Which crystallization solutions are compatible with lipidic cubic phase crystallization?

FAQ ID -2418

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Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2352

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What species are QuantiTect Probe Assays offered for?

FAQ ID -2367

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Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2348

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Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?

FAQ ID -2383

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Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?

FAQ ID -2364

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Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?

FAQ ID -2317

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Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?

FAQ ID -2338

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Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?

FAQ ID -2300

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Can QuantiFast Probe Assays be ordered offline via e-mail or fax?

FAQ ID -2368

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For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?

FAQ ID -2319

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For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?

FAQ ID -2339

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Does the KRAS PCR kit cover the codon 61 mutation?

FAQ ID -2308

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Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?

FAQ ID -2371

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Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?

FAQ ID -2318

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Are the QuantiFast Probe Assays hydrolysis or FRET probes?

FAQ ID -2363

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Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?

FAQ ID -2334

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Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?

FAQ ID -2333

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Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?

FAQ ID -2379

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Can I do singleplex PCR with the QuantiFast Probe Assays?

FAQ ID -2362

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Are the QuantiFast Probe Assays wet-lab validated?

FAQ ID -2375

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Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?

FAQ ID -2301

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At what temperature can I perform the experiment with the NexTal Cubic Phase kits?

FAQ ID -2323

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How much can the mixed standard ?Ct values vary in the KRAS PCR kit?

FAQ ID -2307

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How many samples can be processed per RNeasy Mini QIAcube kit?

FAQ ID -2332

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How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2353

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How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?

FAQ ID -2357

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How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?

FAQ ID -2344

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How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?

FAQ ID -2351

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How much time can be saved when using the RNeasy Mini QIAcube Kit?

FAQ ID -2335

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How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?

FAQ ID -2341

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How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?

FAQ ID -2354

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How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?

FAQ ID -2440

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How are the genes in each iPSC qBiomarker PCR Array selected?

FAQ ID -2439

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How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?

FAQ ID -2356

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How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?

FAQ ID -2350

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How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?

FAQ ID -2340

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How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?

FAQ ID -2330

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Is a special processing protocol needed?

FAQ ID -2523

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What is the detection limit for the Investigator Quantiplex Kit?

FAQ ID -2572

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Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?

FAQ ID -2524

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The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?

FAQ ID -2555

View

What are the dimensions of the TissueLyser LT?

FAQ ID -2545

View

On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?

FAQ ID -2596

View

What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -2534

View

What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?

FAQ ID -2570

View

What is in the elution buffer in the PAXgene Bone Marrow RNA kit?

FAQ ID -2511

View

What are the storage conditions for the mericon Assay kits?

FAQ ID -2540

View

Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?

-20

View

Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?

FAQ ID -2525

View

Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?

FAQ ID -2531

View

Is the morphology after H&E staining comparable to formalin-fixed samples?

FAQ ID -2526

View

Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?

FAQ ID -2509

View

Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?

FAQ ID -2514

View

Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?

FAQ ID -2568

View

What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?

FAQ ID -2518

View

Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?

FAQ ID -2575

View

What RNA yields are expected with the PAXgene Bone Marrow RNA System?

FAQ ID -2506

View

What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?

FAQ ID -2538

View

What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?

FAQ ID -2543

View

What is the expected amplicon size when using the mericon Assay kits?

FAQ ID -2544

View

What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?

FAQ ID -2560

View

What is the typical amount of tissue used for disruption with the TissueLyser LT?

FAQ ID -2552

View

What kind of sample tubes are recommended to use with the TissueLyser LT?

FAQ ID -2553

View

What is the purity of nucleic acids extracted with the PAXgene Tissue kits?

FAQ ID -2533

View

Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?

FAQ ID -2507

View

Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?

FAQ ID -2501

View

Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?

FAQ ID -2515

View

Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?

FAQ ID -2530

View

Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?

FAQ ID -2502

View

Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?

FAQ ID -2549

View

Can the TissueLyser LT Adapter be removed for cleaning?

FAQ ID -2546

View

Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?

FAQ ID -2595

View

Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?

FAQ ID -2548

View

When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?

FAQ ID -2556

View

When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?

FAQ ID -2594

View

Which human target is used for the quantification in the Investigator Quantiplex Kit?

FAQ ID -2577

View

What sample numbers can be processed on the TissueLyser LT?

FAQ ID -2547

View

What size bead should I use for tougher samples with the TissueLyser LT?

FAQ ID -2554

View

When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?

FAQ ID -2597

View

Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?

FAQ ID -2574

View

Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?

FAQ ID -2569

View

Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?

FAQ ID -2508

View

Are there any accessories required for sample disruption with the TissueLyser LT?

FAQ ID -2551

View

At what temperature should I store the Investigator Quantiplex Kit?

FAQ ID -2578

View

Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?

FAQ ID -2503

View

Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?

FAQ ID -2598

View

Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?

FAQ ID -2512

View

Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?

FAQ ID -2510

View

Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?

FAQ ID -2529

View

How long is the fixation time?

FAQ ID -2519

View

Can HiPerFect HTS be used for miRNA?

FAQ ID -2561

View

Can HiPerFect HTS Reagent be used for transfecting shRNAs?

FAQ ID -2559

View

How long does the sample disruption with the TissueLyser LT take?

FAQ ID -2550

View

How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?

FAQ ID -2599

View

How long can I store the dilutions of the Control DNA Z1 standards at 2°C?

-8

View

How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?

-20

View

How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?

FAQ ID -2505

View

How long does an Investigator Quantiplex run take?

FAQ ID -2566

View

How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?

FAQ ID -2504

View

I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?

FAQ ID -2513

View

Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?

FAQ ID -2571

View

Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?

FAQ ID -2557

View

How can I streamline the quantification workflow for the Investigator QuantiPlex Kit?

FAQ ID -2579

View

How is stability and performance of these mericon Assay kits guaranteed?

FAQ ID -2542

View

How long are the target sequences of Investigator Quantiplex?

FAQ ID -2576

View

For which real-time cyclers has the Investigator Quantiplex Kit been validated?

FAQ ID -2567

View

How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?

FAQ ID -2516

View

What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?

FAQ ID -3055

View

Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?

FAQ ID -3054

View

Why are two reagents used in the PAXgene Tissue System?

FAQ ID -3022

View

Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?

FAQ ID -3095

View

Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?

FAQ ID -3092

View

Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples?

FAQ ID -3091

View

Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?

FAQ ID -3090

View

Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers?

FAQ ID -3089

View

What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?

FAQ ID -3096

View

Can additional positive controls be used for the Investigator IDplex GO! Kit?

FAQ ID -3094

View

Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?

FAQ ID -3088

View

Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit?

FAQ ID -3093

View

What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?

FAQ ID -3024

View

5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?

FAQ ID -3023

View

What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?

FAQ ID -3025

View

Is it possible to fix over a weekend?

FAQ ID -3027

View

How long is the fixation time when using PAXgene Tissue FIX Container?

FAQ ID -3026

View

How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?

FAQ ID -3028

View

What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?

FAQ ID -3029

View

Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?

FAQ ID -3037

View

Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3038

View

Is the morphology after H&E staining comparable to formalin-fixed samples?

FAQ ID -3039

View

Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3040

View

Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3043

View

is the purity of nucleic acids isolated with the PAXgene Tissue Kits?

FAQ ID -3044

View

What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3045

View

What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3046

View

What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?

FAQ ID -3050

View

How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?

FAQ ID -3052

View

What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?

FAQ ID -3051

View

What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3047

View

Does the PAXgene Tissue RNA Kit co-purify small RNAs?

FAQ ID -3048

View

How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

FAQ ID -3049

View

Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?

FAQ ID -3042

View

What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?

FAQ ID -3086

View

What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?

FAQ ID -3083

View

What are the typical yields obtained from Buccal swabs on BioSprint 96?

FAQ ID -3071

View

Where can I find a copy of the .kpf file for a BioSprint protocol?

FAQ ID -3073

View

Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?

FAQ ID -3075

View

Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?

FAQ ID -3079

View

Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?

FAQ ID -3080

View

What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?

FAQ ID -3064

View

How precise is the QIAgility?

FAQ ID -3063

View

How long does it take to set up a 96-well plate PCR reaction on the QIAgility?

FAQ ID -3066

View

Can I use a bar code scanner or reader on the QIAgility?

FAQ ID -3067

View

Can I use a USB drive to transfer protocols to BioSprint?

FAQ ID -3072

View

Can BioSprint 96 be connected to LIMS?

FAQ ID -3070

View

How can I find the run parameters for a BioSprint protocol that I am running?

FAQ ID -3069

View

Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?

FAQ ID -3077

View

Can I use specific primers with the FastLane Kits?

FAQ ID -3078

View

What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?

FAQ ID -3082

View

What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?

FAQ ID -3081

View

What is the concentration of the different Proteinase K solutions sold by QIAGEN?

FAQ ID -3084

View

Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?

FAQ ID -3087

View

Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?

FAQ ID -3085

View

Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?

FAQ ID -3032

View

Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?

FAQ ID -3030

View

Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?

FAQ ID -3036

View

Is a special processing protocol needed?

FAQ ID -3035

View

Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?

FAQ ID -3033

View

Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?

FAQ ID -3034

View

Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?

FAQ ID -3041

View

When should the N2 bottle used with the QIAxcel Advanced System be changed?

FAQ ID -3001

View

Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?

FAQ ID -3000

View

How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?

FAQ ID -3003

View

How do I clean up RNA preparations containing miRNA?

FAQ ID -3002

View

What is the thermal output for a BioRobot MDx and BioRobot Universal?

FAQ ID -3056

View

What is the thermal output for a QIAsymphony SP and AS instrument?

FAQ ID -3057

View

Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?

FAQ ID -3053

View

What is the minimum number of samples that can be extracted on a BioSprint 96?

FAQ ID -3076

View

What is the fixation method used in the PAXgene Tissue System?

FAQ ID -3019

View

What does PAXgene Tissue FIX contain?

FAQ ID -3020

View

Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?

FAQ ID -3098

View

What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?

FAQ ID -1357

View

Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?

FAQ ID -1304

View

I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?

FAQ ID -1366

View

What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?

FAQ ID -1312

View

If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?

FAQ ID -1310

View

How will service work carried out on my QIAGEN BioRobot be documented?

FAQ ID -1331

View

If I cannot order FlexiPlate siRNA online, how do I order?

FAQ ID -1371

View

What information about my gene or siRNA do I need to order FlexiPlate siRNA?

FAQ ID -1365

View

Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?

-3

View

What information is provided with FlexiPlate siRNAs?

FAQ ID -1363

View

Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?

FAQ ID -1355

View

Which formats are available for FlexiPlate siRNA?

FAQ ID -1358

View

Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?

FAQ ID -1334

View

When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?

FAQ ID -1308

View

Which controls can I choose for FlexiPlate siRNA?

FAQ ID -1368

View

Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?

FAQ ID -1328

View

Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?

FAQ ID -1327

View

Which density gel type is contained in the yellow or green MaXtract Tubes?

FAQ ID -1315

View

What is the composition of FlexiPlate siRNA buffer?

FAQ ID -1361

View

Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?

FAQ ID -1336

View

What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?

FAQ ID -1356

View

Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?

FAQ ID -1325

View

Can MaXtract tubes be autoclaved prior to use?

FAQ ID -1305

View

Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?

FAQ ID -1326

View

Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?

FAQ ID -1321

View

Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?

FAQ ID -1352

View

Can several extraction steps be performed in the same MaXtract tube?

FAQ ID -1302

View

Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?

FAQ ID -1309

View

Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?

FAQ ID -1303

View

Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?

FAQ ID -1319

View

Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?

FAQ ID -1369

View

Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?

FAQ ID -1301

View

Can HP Validated siRNAs be ordered on the FlexiPlate?

FAQ ID -1367

View

Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?

FAQ ID -1337

View

How do I resuspend my FlexiPlate siRNAs?

FAQ ID -1360

View

How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?

FAQ ID -1320

View

Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?

FAQ ID -1323

View

Do you have stability data for genomic DNA isolated with the BioSprint Kits?

FAQ ID -1318

View

Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?

FAQ ID -1307

View

Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?

FAQ ID -1317

View

How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?

FAQ ID -1330

View

How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?

FAQ ID -1364

View

How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?

FAQ ID -1354

View

How can I ensure optimal performance of my QIAGEN instrument?

FAQ ID -1329

View

What is the "seed sequence" of a miRNA?

FAQ ID -2262

View

What is the turn around time for the ordering of miScript Target Protector?

FAQ ID -2254

View

What is the sample throughput of Pyrosequencing systems?

FAQ ID -2215

View

What is the processing time for 96 normalizations with the SeqTarget System?

FAQ ID -2237

View

What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?

FAQ ID -2247

View

What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?

FAQ ID -2292

View

What technologies are used by the KRAS and EGFR PCR kits?

FAQ ID -2286

View

What kind of automation can be done with the EasyXtal 15-well Tool?

FAQ ID -2220

View

What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?

FAQ ID -2216

View

What dyes are used in the KRAS and EGFR PCR kits?

FAQ ID -2288

View

What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?

FAQ ID -2298

View

What format are the EasyXtal 15-well tools?

FAQ ID -2219

View

What do I need to provide when ordering a miScript Target Protector?

FAQ ID -2251

View

What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?

FAQ ID -2253

View

What is a miScript Precursor Assay?

FAQ ID -2214

View

What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2203

View

What is a miScript Target Protector?

FAQ ID -2249

View

Which transfection reagent is recommended for transfection of the miScript Target Protectors?

FAQ ID -2252

View

Which variants of the EasyXtal 15-well tool are available?

FAQ ID -2221

View

Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2205

View

Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2204

View

Why does the SeqTarget Prep Protocol require a 2-step procedure?

FAQ ID -2238

View

Why does the miScript Target Protector not induce the degradation process?

FAQ ID -2263

View

Why do I have wavy DNA bands on my agarose gel?

FAQ ID -2260

View

What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?

FAQ ID -2242

View

When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?

FAQ ID -2294

View

What type of samples can I use for the KRAS PCR and EGFR kits?

FAQ ID -2293

View

What voltage should be used to run my agarose gel with the GelPilot Agarose?

FAQ ID -2261

View

Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?

FAQ ID -2202

View

Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?

FAQ ID -2267

View

Does the miScript Target Protector match the target sequence?

FAQ ID -2250

View

Do the KRAS and EGFR PCR kits contain a passive reference dye?

FAQ ID -2295

View

Do I have to order at least 4 FlexiTube siRNA Premixes?

FAQ ID -2266

View

How do I store my FlexiTube siRNA Premix?

FAQ ID -2270

View

How is ARMS technology superior to alternative methods such as dye terminator sequencing?

FAQ ID -2290

View

Effects of low A260/A230 ratios in RNA preparations on downstream applications

FAQ ID -2248

View

How can I check the success of the normalization procedure for the SeqTarget System?

FAQ ID -2245

View

How do I search for all the miScript Target Protectors for one species?

FAQ ID -2255

View

Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?

FAQ ID -2240

View

Can PCR fragments be normalized for the SeqTarget System without a previous purification step?

FAQ ID -2246

View

Can I use a 384 well plate for the KRAS and EGFR PCR kits?

FAQ ID -2296

View

Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?

FAQ ID -2234

View

Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?

FAQ ID -2211

View

Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?

FAQ ID -2244

View

What are EasyXtal 15-well Tools used for?

FAQ ID -2217

View

What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?

FAQ ID -2200

View

Is the SeqTarget System compatible with standard-size PCRs?

FAQ ID -2241

View

What are the amplicon lengths of the KRAS and EGFR PCR kits?

FAQ ID -2289

View

What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2201

View

What does QIAGEN use for a positive control in precursor detection experiments?

FAQ ID -2212

View

What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?

FAQ ID -2291

View

What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?

FAQ ID -2297

View

What are the corresponding QIAGEN names for former Biotage instruments?

FAQ ID -2285

View

What buffer conditions give the best resolution for agarose gel electrophoresis?

FAQ ID -2256

View

What can be the reason for low DNA yields after normalization with the SeqTarget System?

FAQ ID -2239

View

How much buffer should be used for agarose gel electrophoresis?

FAQ ID -2257

View

How much DNA should be loaded per well of an agarose gel?

FAQ ID -2259

View

How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?

FAQ ID -2258

View

How long does it take to generate results using the KRAS and EGFR PCR kits?

FAQ ID -2299

View

How long should PCR fragments be for the SeqTarget System?

FAQ ID -2236

View

How many miScript Precursor Assays do you offer?

FAQ ID -2213

View

Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?

FAQ ID -2264

View

Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?

FAQ ID -2268

View

Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?

FAQ ID -2269

View

If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?

FAQ ID -2265

View

Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?

FAQ ID -2235

View

Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?

FAQ ID -2243

View

Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?

FAQ ID -1484

View

Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?

FAQ ID -1451

View

What applications are offered on the QIAcube?

FAQ ID -1403

View

Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?

FAQ ID -1449

View

Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?

FAQ ID -1446

View

Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?

FAQ ID -1447

View

Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?

FAQ ID -1442

View

What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?

FAQ ID -1468

View

Can purification columns from other suppliers be processed on the QIAcube?

FAQ ID -1406

View

Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?

FAQ ID -1414

View

Are run reports and/or log files available on the QIAcube?

FAQ ID -1412

View

Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?

FAQ ID -1480

View

Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?

FAQ ID -1476

View

Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?

FAQ ID -1452

View

Can different amounts of siRNA be ordered on one FlexiPlate?

FAQ ID -1473

View

Can 2 µl reaction volumes be used with QuantiFast PCR Kits?

FAQ ID -1440

View

Are there any specific cleaning solutions recommended for the QIAcube?

FAQ ID -1416

View

Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?

FAQ ID -1439

View

Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?

FAQ ID -1437

View

Do I need to buy special kits for the QIAcube?

FAQ ID -1402

View

Do I need to discard partially used QIAcube tip racks?

FAQ ID -1418

View

What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?

FAQ ID -1463

View

What are the turnaround times for FlexiPlate siRNA orders?

FAQ ID -1478

View

What are the dimensions and weight of the QIAcube?

FAQ ID -1407

View

How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?

FAQ ID -1438

View

How many reactions can I perform with the new QuantiFast Kits for real-time PCR?

FAQ ID -1425

View

If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?

FAQ ID -1477

View

How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?

FAQ ID -1430

View

Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

FAQ ID -1415

View

Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?

FAQ ID -1450

View

Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?

FAQ ID -1443

View

What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?

FAQ ID -1436

View

What is the detection limit of the QuantiFast Kits for real-time PCR?

FAQ ID -1432

View

Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?

FAQ ID -1459

View

Which cell lines have been tested with FastLane Cell RT-PCR Kits?

FAQ ID -1485

View

Can I adjust the ROX concentration in the QuantiFast master mix?

FAQ ID -1427

View

Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?

FAQ ID -1458

View

Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?

FAQ ID -1482

View

Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?

FAQ ID -1433

View

Can FlexiPlate siRNA be ordered in solution on dry-ice?

FAQ ID -1474

View

Can I program my own protocols for the QIAcube?

FAQ ID -1405

View

How can I load new protocols onto the QIAcube?

FAQ ID -1421

View

How can I get software updates for the QIAcube?

FAQ ID -1413

View

How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?

FAQ ID -1441

View

Do you offer trial-kit sizes for the new QuantiFast Kits?

FAQ ID -1429

View

How can I decontaminate the QIAcube system?

FAQ ID -1419

View

Can the QIAcube heater/shaker be used independently from protocol runs?

FAQ ID -1422

View

Can the QIAcube rotor adapters be autoclaved?

FAQ ID -1409

View

Can the QIAcube centrifuge be used independently from protocol runs?

FAQ ID -1423

View

Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?

FAQ ID -1428

View

Can the QIAcube be connected to a laboratory information management system?

FAQ ID -1411

View

Can the QIAcube rotor and/or buckets be removed for cleaning?

FAQ ID -1410

View

Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?

FAQ ID -1444

View

Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?

FAQ ID -1431

View

Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?

FAQ ID -1435

View

What are the Tms of PCR products obtained using the miScript System?

FAQ ID -1599

View

Do you have a protocol for cleanup of REPLI-g amplified DNA?

FAQ ID -1545

View

What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1580

View

What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?

FAQ ID -1596

View

What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?

FAQ ID -1554

View

Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?

FAQ ID -1553

View

Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?

FAQ ID -1572

View

Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?

-8

View

Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?

FAQ ID -1573

View

Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?

FAQ ID -1587

View

Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?

FAQ ID -1547

View

Do you have a protocol for concentration of RNA in 96-well plates?

FAQ ID -1546

View

Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

FAQ ID -1550

View

Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?

FAQ ID -1592

View

What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?

FAQ ID -1584

View

What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1591

View

Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?

FAQ ID -1579

View

What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?

FAQ ID -1597

View

Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?

FAQ ID -1575

View

Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?

FAQ ID -1555

View

Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1583

View

Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?

FAQ ID -1576

View

Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1577

View

Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?

FAQ ID -1585

View

Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?

FAQ ID -1571

View

Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1590

View

Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?

FAQ ID -1574

View

Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1586

View

How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?

FAQ ID -1598

View

How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

FAQ ID -1519

View

What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?

FAQ ID -2010

View

What is the advantage of using the Type-it Microsatellite PCR Kit?

FAQ ID -2059

View

What is the advantage of using the Type-it Mutation Detect PCR Kit?

FAQ ID -2064

View

What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?

FAQ ID -2005

View

What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?

FAQ ID -2029

View

What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?

-16

View

What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?

FAQ ID -2028

View

What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?

FAQ ID -2003

View

Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?

FAQ ID -2072

View

What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?

FAQ ID -2007

View

Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?

FAQ ID -2073

View

What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?

FAQ ID -2009

View

Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?

FAQ ID -2031

View

What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?

FAQ ID -2011

View

What are the features of the expression vector used for QIAgenes Insect/Mammalia?

FAQ ID -2050

View

Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?

FAQ ID -2035

View

Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?

FAQ ID -2068

View

Why is there more than one QIAgene for my gene of interest?

FAQ ID -2044

View

Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?

FAQ ID -2043

View

Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?

FAQ ID -2067

View

Will the optimized DNA sequence for QIAgenes be provided?

FAQ ID -2045

View

Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?

FAQ ID -2060

View

Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?

FAQ ID -2057

View

What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?

FAQ ID -2037

View

What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?

FAQ ID -2036

View

Can data be analyzed on the Rotor-Gene Q while a run is in progress?

FAQ ID -2091

View

Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?

FAQ ID -2048

View

Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?

FAQ ID -2066

View

Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?

FAQ ID -2058

View

Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?

FAQ ID -2030

View

Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?

FAQ ID -2079

View

Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?

FAQ ID -2092

View

Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?

FAQ ID -2093

View

What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?

FAQ ID -2004

View

What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?

FAQ ID -2034

View

Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?

FAQ ID -2049

View

Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?

FAQ ID -2038

View

What types of reaction vessels are required for use in the Rotor-Gene Q?

FAQ ID -2085

View

Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?

FAQ ID -2063

View

Can the EpiTect PCR Control DNAs be used for mouse and rat assays?

FAQ ID -2008

View

Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?

FAQ ID -2006

View

Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?

FAQ ID -2001

View

Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?

FAQ ID -2027

View

Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?

-15

View

Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?

FAQ ID -2074

View

Do all QIAsymphony DNA Investigator protocols use carrier RNA?

FAQ ID -2033

View

Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?

FAQ ID -2052

View

Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?

FAQ ID -2065

View

Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?

FAQ ID -2062

View

Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?

FAQ ID -2082

View

How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?

FAQ ID -2083

View

Can membrane proteins be expressed with QIAgenes expression kits?

FAQ ID -2047

View

Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?

FAQ ID -2000

View

Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?

FAQ ID -2040

View

Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?

FAQ ID -2039

View

Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?

FAQ ID -2081

View

How can a C-terminal 6xHis-tagged QIAgene be expressed?

FAQ ID -2041

View

How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?

FAQ ID -2051

View

Does the Rotor-Gene Q software have the capacity to export data?

FAQ ID -2089

View

Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?

FAQ ID -2032

View

Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?

FAQ ID -2070

View

How fast does the rotor spin during a run on the Rotor-Gene Q?

FAQ ID -2080

View

How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?

FAQ ID -2088

View

How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?

FAQ ID -2002

View

How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?

FAQ ID -2095

View

How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?

FAQ ID -2071

View

If orders cannot be placed online, how can QIAgenes be ordered?

FAQ ID -2046

View

If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?

FAQ ID -2087

View

How long does a single run on the PyroMark Q24 take?

FAQ ID -2096

View

How much space does the PyroMark Q24 instrument need?

FAQ ID -2098

View

Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?

FAQ ID -2061

View

How accurate and reliable is PyroMark Q24 in mutation analysis?

FAQ ID -2094

View

Is there a charge for user training on the QIAsymphony SP System?

FAQ ID -1900

View

Is the QIAsymphony RNA Kit compatible with stabilized samples?

FAQ ID -1944

View

Is sample pretreatment required for purifying RNA on the QIAsymphony SP?

FAQ ID -1938

View

Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?

FAQ ID -1920

View

What are the expected DNA yields from tissue processed on the QIAsymphony SP?

FAQ ID -1919

View

QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?

FAQ ID -1982

View

What are the dimensions and weight of the QIAsymphony SP System?

FAQ ID -1901

View

What is the range of elution volumes on the QIAsymphony SP?

FAQ ID -1908

View

How many PCR reactions can be performed for different scales of miScript Primer Assays?

FAQ ID -1990

View

How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?

FAQ ID -1940

View

How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?

FAQ ID -1905

View

How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?

FAQ ID -1994

View

How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?

FAQ ID -1941

View

How much sample can be used with the Allprep DNA/RNA 96 Kit?

FAQ ID -1997

View

How much sample can be used with the RNeasy Plus 96 Kit?

FAQ ID -1992

View

How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?

FAQ ID -1917

View

What sample types can be used with the AllPrep DNA/RNA 96 Kit?

FAQ ID -1996

View

Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?

FAQ ID -1937

View

What sample types can be used with the RNeasy Plus 96 Kit?

FAQ ID -1991

View

Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?

FAQ ID -1921

View

What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?

FAQ ID -1918

View

Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?

FAQ ID -1999

View

Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?

FAQ ID -1993

View

What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?

FAQ ID -1985

View

Is it possible to modify QIAsymphony protocols?

FAQ ID -1903

View

What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?

FAQ ID -1939

View

What are the expected yields of total RNA isolated with the QIAsymphony SP?

FAQ ID -1942

View

What kind of molecules are miScript miRNA Inhibitors?

FAQ ID -1983

View

What sample input volumes can be used on the QIAsymphony SP?

FAQ ID -1907

View

What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?

FAQ ID -1915

View

What kind of molecules are miScript miRNA Mimics?

FAQ ID -1986

View

What RNA elution volumes are available with the QIAsymphony RNA Kit?

FAQ ID -1943

View

Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

FAQ ID -1916

View

Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?

FAQ ID -1976

View

Can uneven sample numbers be processed on the QIAsymphony SP module?

FAQ ID -1906

View

Does the QIAsymphony SP system generate run reports and/or log files?

FAQ ID -1911

View

Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?

FAQ ID -1984

View

Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?

FAQ ID -1964

View

Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?

FAQ ID -1953

View

Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?

FAQ ID -1960

View

Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?

FAQ ID -1969

View

Can I program my own protocols for the QIAsymphony SP?

FAQ ID -1904

View

Are QuantiFast Multiplex PCR Kits available in trial sizes?

FAQ ID -1981

View

Are there any specific cleaning solutions recommended for the QIAsymphony SP?

FAQ ID -1913

View

Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?

FAQ ID -1978

View

Can the QIAsymphony RNA Kit be used to purify RNA from blood?

FAQ ID -1945

View

Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?

FAQ ID -1909

View

Can protocol runs on the QIAsymphony SP be interrupted and resumed?

FAQ ID -1912

View

Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?

FAQ ID -1979

View

Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?

FAQ ID -1995

View

Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?

FAQ ID -1950

View

Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?

FAQ ID -1951

View

Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?

FAQ ID -1959

View

Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?

FAQ ID -1975

View

Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?

FAQ ID -1946

View

Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?

FAQ ID -1947

View

Do you have a protocol for the purification of DNA from fruit flies?

FAQ ID -1970

View

Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?

FAQ ID -1974

View

How can the QIAsymphony SP system be decontaminated?

FAQ ID -1914

View

Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?

FAQ ID -1958

View

Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?

FAQ ID -1955

View

Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?

FAQ ID -1954

View

Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?

FAQ ID -1967

View

Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?

FAQ ID -1968

View

Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?

FAQ ID -1948

View

Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?

FAQ ID -1972

View

Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?

FAQ ID -1973

View

Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?

FAQ ID -1971

View

Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?

FAQ ID -1965

View

Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?

FAQ ID -1952

View

What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?

FAQ ID -1751

View

What is the fidelity of TopTaq DNA Polymerase?

FAQ ID -1739

View

Is TopTaq DNA Polymerase a hot-start polymerase?

FAQ ID -1748

View

Which nucleotide analogs can be used with TopTaq DNA Polymerase?

FAQ ID -1741

View

Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?

FAQ ID -1755

View

What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?

FAQ ID -1738

View

What is the shelf-life of TopTaq DNA Polymerase and Master Mix?

FAQ ID -1743

View

What is the principle of the REPLI-g FFPE Kit?

FAQ ID -1753

View

What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?

FAQ ID -1752

View

What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?

FAQ ID -1750

View

Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?

FAQ ID -1737

View

What is the recommended sample size for use with the REPLI-g FFPE Kit?

FAQ ID -1756

View

Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?

FAQ ID -1749

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Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?

FAQ ID -1702

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Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?

FAQ ID -1740

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At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?

FAQ ID -1758

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Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?

FAQ ID -1714

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How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?

FAQ ID -1734

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Do you have protocols for sample processing on the Autopure LS instrument?

FAQ ID -1759

View

Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?

FAQ ID -1754

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Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?

FAQ ID -1760

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Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?

FAQ ID -1733

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Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?

FAQ ID -1744

View

Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?

FAQ ID -1761

View

Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?

FAQ ID -1745

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Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?

FAQ ID -1666

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What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?

FAQ ID -1601

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What is the binding capacity of the Ni-NTA Superflow Cartridges?

FAQ ID -1603

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Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1610

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Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?

FAQ ID -1697

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Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?

FAQ ID -1609

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Is sequence information for HP Validated siRNAs provided in the FlexiTube format?

FAQ ID -1665

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Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1614

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Which siRNA controls are available in FlexiTube format?

FAQ ID -1661

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Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?

FAQ ID -1690

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Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?

FAQ ID -1620

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What is the composition of Buffer PE?

FAQ ID -1691

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What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1616

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Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?

FAQ ID -1606

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Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?

FAQ ID -1607

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Can I store cDNA generated by the miScript System?

FAQ ID -1602

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Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?

FAQ ID -1612

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Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?

FAQ ID -1615

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Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?

FAQ ID -1617

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Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?

FAQ ID -1618

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Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?

FAQ ID -1664

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Can I still reorder siRNA that has been removed from the GeneGlobe data base?

FAQ ID -1668

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Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?

FAQ ID -1608

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Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?

FAQ ID -1667

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Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?

FAQ ID -1658

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Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?

FAQ ID -1611

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How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?

FAQ ID -1644

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How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?

FAQ ID -1605

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How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1613

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How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1623

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Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?

FAQ ID -1619

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Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?

FAQ ID -1659

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Do you have a protocol for purification of total RNA from plant latex?

FAQ ID -1694

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Do I need to use the miScript Universal Primer for detection of mRNA?

FAQ ID -1600

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Do you offer a QuantiFast Kit for one-step RT-PCR?

FAQ ID -1695

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Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?

FAQ ID -1693

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How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?

FAQ ID -2159

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How often can the Strep-Tactin Superflow Plus resin be reused?

FAQ ID -2169

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If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2129

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If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?

FAQ ID -2150

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Is it normal that the binding capacity of GST-resins decreases with flow rate?

FAQ ID -2174

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Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?

FAQ ID -2172

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Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?

FAQ ID -2196

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Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?

FAQ ID -2118

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Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?

FAQ ID -2122

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Why do I need normalization using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2198

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Why is there an asterisk for my miScript Primer Assays?

FAQ ID -2176

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Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?

FAQ ID -2121

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Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?

FAQ ID -2139

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Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?

FAQ ID -2124

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What is miRNA*? Do I need to consider this in my profiling experiment?

FAQ ID -2179

View

What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?

FAQ ID -2119

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What are possible reasons for reduced DNA yields with REPLI-g Kits?

FAQ ID -2148

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What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2199

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What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2158

View

What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?

FAQ ID -2109

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What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?

FAQ ID -2144

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What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?

FAQ ID -2145

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How should DNA isolated on the QIAsymphony be stored?

FAQ ID -2165

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How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2157

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How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?

FAQ ID -2193

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Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?

FAQ ID -2188

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Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?

FAQ ID -2173

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Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?

FAQ ID -2160

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What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2156

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Which plate formats are offered for QuantiTect Primer Assay Plates?

FAQ ID -2106

View

What species are QuantiTect Primer Assay Plates offered for?

FAQ ID -2110

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Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?

FAQ ID -2147

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Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?

FAQ ID -2154

View

How many reactions can be performed per well of the QuantiTect Primer Assay Plates?

FAQ ID -2107

View

Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?

FAQ ID -2190

View

Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?

FAQ ID -2108

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Can Glutathione Superflow resin be reused?

FAQ ID -2171

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Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?

FAQ ID -2138

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Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?

FAQ ID -2112

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Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?

FAQ ID -2168

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Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?

FAQ ID -2134

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Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2130

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2131

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Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?

FAQ ID -2142

View

Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?

FAQ ID -2135

View

Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?

FAQ ID -2191

View

Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

FAQ ID -2116

View

Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?

FAQ ID -2136

View

Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?

FAQ ID -2111

View

Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?

FAQ ID -2126

View

How does template quality influence the results with the Type-it HRM PCR Kit?

FAQ ID -2197

View

How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?

FAQ ID -2166

View

Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?

FAQ ID -2105

View

How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?

FAQ ID -2189

View

How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?

FAQ ID -2125

View

How many elution steps are recommended when using Glutathione Superflow resin?

FAQ ID -2175

View

How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?

FAQ ID -2192

View

Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?

FAQ ID -2104

View

Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2163

View

Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2162

View

Can Vapor-Lock be used with samples processed on the QIAgility?

FAQ ID -2153

View

Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?

FAQ ID -2123

View

Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?

FAQ ID -2117

View

Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2128

View

What main input voltage is required for the QIAxcel Analyzer?

FAQ ID -1821

View

What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

FAQ ID -1852

View

Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?

FAQ ID -1882

View

The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?

FAQ ID -1850

View

Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?

FAQ ID -1832

View

What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?

FAQ ID -1834

View

Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??

FAQ ID -1847

View

Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?

FAQ ID -1844

View

Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?

FAQ ID -1851

View

Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?

FAQ ID -1828

View

Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?

FAQ ID -1841

View

Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?

FAQ ID -1861

View

How can a positive threshold be set when running the QIAxcel System?

FAQ ID -1843

View

How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System?

FAQ ID -1827

View

How long will the intensity calibration procedure on the QIAxcel System take?

FAQ ID -1826

View

Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?

FAQ ID -1824

View

Will the QIAxcel System run using a green Data Review Software Key?

FAQ ID -1853

View

Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?

FAQ ID -1848

View

Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?

FAQ ID -1835

View

When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?

FAQ ID -1842

View

What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?

FAQ ID -1833

View

Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?

FAQ ID -1837

View

When should an intensity calibration be re-performed on the QIAxcel System?

FAQ ID -1825

View

When should the N2 bottle used with the QIAxcel System be changed?

FAQ ID -1830

View

Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?

FAQ ID -1855

View

Can the QIAxcel System or QIAxcel Advanced be connected to a network?

FAQ ID -1862

View

How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?

FAQ ID -1836

View

Can multiple QIAxcel Systems be run from one computer?

FAQ ID -1860

View

If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?

FAQ ID -1858

View

If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?

FAQ ID -1857

View

How often should the alignment marker for the QIAxcel System be changed?

FAQ ID -1839

View

How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?

FAQ ID -1840

View

In the results table generated with the QIAxcel System, what does "normalized area" mean?

FAQ ID -1854

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How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?

FAQ ID -1823

View

If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?

FAQ ID -1856

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How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?

FAQ ID -1838

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What is the composition of buffer QBT?

FAQ ID -411

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What is the composition of buffer QC?

FAQ ID -412

View

What is the composition of buffer P3?

FAQ ID -418

View

What is the composition of buffer QF?

FAQ ID -413

View

What is the composition of buffer TE?

FAQ ID -416

View

What is the composition of buffer QN?

FAQ ID -414

View

What is the composition of buffer OCD?

FAQ ID -423

View

What is the composition of buffer FWB2?

FAQ ID -417

View

What is the composition of buffer OBB?

FAQ ID -421

View

What is the composition of buffer OW2?

FAQ ID -419

View

What is the composition of buffer OL2?

FAQ ID -424

View

What is the composition of buffer OCL?

FAQ ID -422

View

Can RNAi experiments be performed in Drosophila?

FAQ ID -436

View

What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?

FAQ ID -470

View

Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?

FAQ ID -472

View

Has RNAi been successful using siRNA in Zebrafish and Xenopus?

FAQ ID -400

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How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?

FAQ ID -468

View

How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?

FAQ ID -498

View

Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?

FAQ ID -403

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Can QIAquick Kits be used to clean up RNA samples?

FAQ ID -490

View

Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?

FAQ ID -482

View

Can Ni-NTA resins be used to purify protein with an internal His-tag?

FAQ ID -496

View

What is the composition of buffer STE?

FAQ ID -415

View

Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?

FAQ ID -429

View

What is the composition of buffer OEB?

FAQ ID -420

View

Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?

FAQ ID -474

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Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?

FAQ ID -467

View

Why is the pQE DNA provided in QIAexpress Kits blue in color?

FAQ ID -487

View

Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?

FAQ ID -473

View

What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?

FAQ ID -432

View

What is the minimum elution volume when using the RNeasy Micro Kit?

FAQ ID -428

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What is the smallest sample size that can be used with RNeasy Mini Kits?

FAQ ID -485

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