Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance What kind of automation can be done with the EasyXtal 15-well Tool? FAQ ID -2220 View What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis? FAQ ID -2216 View What dyes are used in the KRAS and EGFR PCR kits? FAQ ID -2288 View What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits? FAQ ID -2298 View What format are the EasyXtal 15-well tools? FAQ ID -2219 View What do I need to provide when ordering a miScript Target Protector? FAQ ID -2251 View What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector? FAQ ID -2253 View What is a miScript Precursor Assay? FAQ ID -2214 View What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2203 View What is a miScript Target Protector? FAQ ID -2249 View Which transfection reagent is recommended for transfection of the miScript Target Protectors? FAQ ID -2252 View Which variants of the EasyXtal 15-well tool are available? FAQ ID -2221 View Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software? FAQ ID -2205 View Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software? FAQ ID -2204 View Why does the SeqTarget Prep Protocol require a 2-step procedure? FAQ ID -2238 View Why does the miScript Target Protector not induce the degradation process? FAQ ID -2263 View Why do I have wavy DNA bands on my agarose gel? FAQ ID -2260 View What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2? FAQ ID -2242 View When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them? FAQ ID -2294 View What type of samples can I use for the KRAS PCR and EGFR kits? FAQ ID -2293 View What voltage should be used to run my agarose gel with the GelPilot Agarose? FAQ ID -2261 View Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised? FAQ ID -2202 View Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance? FAQ ID -2267 View Does the miScript Target Protector match the target sequence? FAQ ID -2250 View Do the KRAS and EGFR PCR kits contain a passive reference dye? FAQ ID -2295 View Do I have to order at least 4 FlexiTube siRNA Premixes? FAQ ID -2266 View How do I store my FlexiTube siRNA Premix? FAQ ID -2270 View How is ARMS technology superior to alternative methods such as dye terminator sequencing? FAQ ID -2290 View Effects of low A260/A230 ratios in RNA preparations on downstream applications FAQ ID -2248 View How can I check the success of the normalization procedure for the SeqTarget System? FAQ ID -2245 View How do I search for all the miScript Target Protectors for one species? FAQ ID -2255 View Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick? FAQ ID -2240 View Can PCR fragments be normalized for the SeqTarget System without a previous purification step? FAQ ID -2246 View Can I use a 384 well plate for the KRAS and EGFR PCR kits? FAQ ID -2296 View Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used? FAQ ID -2234 View Can the miScript Precursor Assay differentiate between pri- and pre-miRNA? FAQ ID -2211 View Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System? FAQ ID -2244 View What are EasyXtal 15-well Tools used for? FAQ ID -2217 View What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software? FAQ ID -2200 View Is the SeqTarget System compatible with standard-size PCRs? FAQ ID -2241 View What are the amplicon lengths of the KRAS and EGFR PCR kits? FAQ ID -2289 View What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2201 View What does QIAGEN use for a positive control in precursor detection experiments? FAQ ID -2212 View What controls are used in the KRAS and EGFR PCR kits to ensure reliable results? FAQ ID -2291 View What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits? FAQ ID -2297 View What are the corresponding QIAGEN names for former Biotage instruments? FAQ ID -2285 View What buffer conditions give the best resolution for agarose gel electrophoresis? FAQ ID -2256 View What can be the reason for low DNA yields after normalization with the SeqTarget System? FAQ ID -2239 View How much buffer should be used for agarose gel electrophoresis? FAQ ID -2257 View How much DNA should be loaded per well of an agarose gel? FAQ ID -2259 View How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved? FAQ ID -2258 View How long does it take to generate results using the KRAS and EGFR PCR kits? FAQ ID -2299 View How long should PCR fragments be for the SeqTarget System? FAQ ID -2236 View How many miScript Precursor Assays do you offer? FAQ ID -2213 View Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix? FAQ ID -2264 View Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types? FAQ ID -2268 View Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types? FAQ ID -2269 View If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do? FAQ ID -2265 View Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed? FAQ ID -2235 View Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System? FAQ ID -2243 View Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits? FAQ ID -1484 View Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits? FAQ ID -1451 View What applications are offered on the QIAcube? FAQ ID -1403 View Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits? FAQ ID -1449 View Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits? FAQ ID -1446 View Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits? FAQ ID -1447 View Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers? FAQ ID -1442 View What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates? FAQ ID -1468 View Can purification columns from other suppliers be processed on the QIAcube? FAQ ID -1406 View Can QIAcube protocol runs be interrupted, and subsequently be continued to completion? FAQ ID -1414 View Are run reports and/or log files available on the QIAcube? FAQ ID -1412 View Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR? FAQ ID -1480 View Are FlexiPlate siRNAs available at a higher final concentration than 10 µM? FAQ ID -1476 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits? FAQ ID -1452 View Can different amounts of siRNA be ordered on one FlexiPlate? FAQ ID -1473 View Can 2 µl reaction volumes be used with QuantiFast PCR Kits? FAQ ID -1440 View Are there any specific cleaning solutions recommended for the QIAcube? FAQ ID -1416 View Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits? FAQ ID -1439 View Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex? FAQ ID -1437 View Do I need to buy special kits for the QIAcube? FAQ ID -1402 View Do I need to discard partially used QIAcube tip racks? FAQ ID -1418 View What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format? FAQ ID -1463 View What are the turnaround times for FlexiPlate siRNA orders? FAQ ID -1478 View What are the dimensions and weight of the QIAcube? FAQ ID -1407 View How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits? FAQ ID -1438 View How many reactions can I perform with the new QuantiFast Kits for real-time PCR? FAQ ID -1425 View If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs? FAQ ID -1477 View How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling? FAQ ID -1430 View Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube? FAQ ID -1415 View Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits? FAQ ID -1450 View Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration? FAQ ID -1443 View What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex? FAQ ID -1436 View What is the detection limit of the QuantiFast Kits for real-time PCR? FAQ ID -1432 View Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs? FAQ ID -1459 View Which cell lines have been tested with FastLane Cell RT-PCR Kits? FAQ ID -1485 View Can I adjust the ROX concentration in the QuantiFast master mix? FAQ ID -1427 View Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates? FAQ ID -1458 View Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates? FAQ ID -1482 View Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes? FAQ ID -1433 View Can FlexiPlate siRNA be ordered in solution on dry-ice? FAQ ID -1474 View Can I program my own protocols for the QIAcube? FAQ ID -1405 View How can I load new protocols onto the QIAcube? FAQ ID -1421 View How can I get software updates for the QIAcube? FAQ ID -1413 View How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR? FAQ ID -1441 View Do you offer trial-kit sizes for the new QuantiFast Kits? FAQ ID -1429 View How can I decontaminate the QIAcube system? FAQ ID -1419 View Can the QIAcube heater/shaker be used independently from protocol runs? FAQ ID -1422 View Can the QIAcube rotor adapters be autoclaved? FAQ ID -1409 View Can the QIAcube centrifuge be used independently from protocol runs? FAQ ID -1423 View Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options? FAQ ID -1428 View Can the QIAcube be connected to a laboratory information management system? FAQ ID -1411 View Can the QIAcube rotor and/or buckets be removed for cleaning? FAQ ID -1410 View Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity? FAQ ID -1444 View Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments? FAQ ID -1431 View Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye? FAQ ID -1435 View What are the Tms of PCR products obtained using the miScript System? FAQ ID -1599 View Do you have a protocol for cleanup of REPLI-g amplified DNA? FAQ ID -1545 View What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1580 View What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System? FAQ ID -1596 View What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits? FAQ ID -1554 View Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits? FAQ ID -1553 View Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent? FAQ ID -1572 View Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol? -8 View Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification? FAQ ID -1573 View Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis? FAQ ID -1587 View Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit? FAQ ID -1547 View Do you have a protocol for concentration of RNA in 96-well plates? FAQ ID -1546 View Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density? FAQ ID -1550 View Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit? FAQ ID -1592 View What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure? FAQ ID -1584 View What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit? FAQ ID -1591 View Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues? FAQ ID -1579 View What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System? FAQ ID -1597 View Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein? FAQ ID -1575 View Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers? FAQ ID -1555 View Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1583 View Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone? FAQ ID -1576 View Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit? FAQ ID -1577 View Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts? FAQ ID -1585 View Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies? FAQ ID -1571 View Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1590 View Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants? FAQ ID -1574 View Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1586 View How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System? FAQ ID -1598 View How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument? FAQ ID -1519 View What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies? FAQ ID -2010 View What is the advantage of using the Type-it Microsatellite PCR Kit? FAQ ID -2059 View What is the advantage of using the Type-it Mutation Detect PCR Kit? FAQ ID -2064 View What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit? FAQ ID -2005 View What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit? FAQ ID -2029 View What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K? -16 View What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP? FAQ ID -2028 View What is the principle behind the DNA Polymerase in the EpiTect MSP Kit? FAQ ID -2003 View Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit? FAQ ID -2072 View What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007 View Is magnetic particle carry-over in the eluates on the BioSprint 96 normal? FAQ ID -2073 View What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template? FAQ ID -2009 View Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP? FAQ ID -2031 View What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA? FAQ ID -2011 View What are the features of the expression vector used for QIAgenes Insect/Mammalia? FAQ ID -2050 View Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)? FAQ ID -2035 View Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit? FAQ ID -2068 View Why is there more than one QIAgene for my gene of interest? FAQ ID -2044 View Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon? FAQ ID -2043 View Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs? FAQ ID -2067 View Will the optimized DNA sequence for QIAgenes be provided? FAQ ID -2045 View Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp? FAQ ID -2060 View Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping? FAQ ID -2057 View What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits? FAQ ID -2037 View What sample types can be processed using the QIAsymphony Virus/Bacteria protocols? FAQ ID -2036 View Can data be analyzed on the Rotor-Gene Q while a run is in progress? FAQ ID -2091 View Are there two vectors for protein expression using QIAgenes in insect or mammalian cells? FAQ ID -2048 View Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit? FAQ ID -2066 View Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit? FAQ ID -2058 View Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP? FAQ ID -2030 View Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results? FAQ ID -2079 View Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run? FAQ ID -2092 View Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q? FAQ ID -2093 View What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit? FAQ ID -2004 View What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit? FAQ ID -2034 View Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs? FAQ ID -2049 View Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol? FAQ ID -2038 View What types of reaction vessels are required for use in the Rotor-Gene Q? FAQ ID -2085 View Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits? FAQ ID -2063 View Can the EpiTect PCR Control DNAs be used for mouse and rat assays? FAQ ID -2008 View Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification? FAQ ID -2006 View Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples? FAQ ID -2001 View Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument? FAQ ID -2027 View Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models? -15 View Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments? FAQ ID -2074 View Do all QIAsymphony DNA Investigator protocols use carrier RNA? FAQ ID -2033 View Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months? FAQ ID -2052 View Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp? FAQ ID -2065 View Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates? FAQ ID -2062 View Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled? FAQ ID -2082 View How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q? FAQ ID -2083 View Can membrane proteins be expressed with QIAgenes expression kits? FAQ ID -2047 View Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure? FAQ ID -2000 View Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run? FAQ ID -2040 View Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument? FAQ ID -2039 View Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q? FAQ ID -2081 View How can a C-terminal 6xHis-tagged QIAgene be expressed? FAQ ID -2041 View How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned? FAQ ID -2051 View Does the Rotor-Gene Q software have the capacity to export data? FAQ ID -2089 View Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL? FAQ ID -2032 View Can frozen samples be used with the MagAttract 96 cador Pathogen Kit? FAQ ID -2070 View How fast does the rotor spin during a run on the Rotor-Gene Q? FAQ ID -2080 View How is the log graph threshold value (Ct) for the Rotor-Gene Q determined? FAQ ID -2088 View How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit? FAQ ID -2002 View How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24? FAQ ID -2095 View How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware? FAQ ID -2071 View If orders cannot be placed online, how can QIAgenes be ordered? FAQ ID -2046 View If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes? FAQ ID -2087 View How long does a single run on the PyroMark Q24 take? FAQ ID -2096 View How much space does the PyroMark Q24 instrument need? FAQ ID -2098 View Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used? FAQ ID -2061 View How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094 View Is there a charge for user training on the QIAsymphony SP System? FAQ ID -1900 View Is the QIAsymphony RNA Kit compatible with stabilized samples? FAQ ID -1944 View Is sample pretreatment required for purifying RNA on the QIAsymphony SP? FAQ ID -1938 View Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols? FAQ ID -1920 View What are the expected DNA yields from tissue processed on the QIAsymphony SP? FAQ ID -1919 View QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean? FAQ ID -1982 View What are the dimensions and weight of the QIAsymphony SP System? FAQ ID -1901 View What is the range of elution volumes on the QIAsymphony SP? FAQ ID -1908 View How many PCR reactions can be performed for different scales of miScript Primer Assays? FAQ ID -1990 View How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP? FAQ ID -1940 View How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle? FAQ ID -1905 View How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate? FAQ ID -1994 View How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation? FAQ ID -1941 View How much sample can be used with the Allprep DNA/RNA 96 Kit? FAQ ID -1997 View How much sample can be used with the RNeasy Plus 96 Kit? FAQ ID -1992 View How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP? FAQ ID -1917 View What sample types can be used with the AllPrep DNA/RNA 96 Kit? FAQ ID -1996 View Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP? FAQ ID -1937 View What sample types can be used with the RNeasy Plus 96 Kit? FAQ ID -1991 View Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP? FAQ ID -1921 View What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP? FAQ ID -1918 View Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus? FAQ ID -1999 View Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit? FAQ ID -1993 View What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays? FAQ ID -1985 View Is it possible to modify QIAsymphony protocols? FAQ ID -1903 View What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP? FAQ ID -1939 View What are the expected yields of total RNA isolated with the QIAsymphony SP? FAQ ID -1942 View What kind of molecules are miScript miRNA Inhibitors? FAQ ID -1983 View What sample input volumes can be used on the QIAsymphony SP? FAQ ID -1907 View What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP? FAQ ID -1915 View What kind of molecules are miScript miRNA Mimics? FAQ ID -1986 View What RNA elution volumes are available with the QIAsymphony RNA Kit? FAQ ID -1943 View Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? FAQ ID -1916 View Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits? FAQ ID -1976 View Can uneven sample numbers be processed on the QIAsymphony SP module? FAQ ID -1906 View Does the QIAsymphony SP system generate run reports and/or log files? FAQ ID -1911 View Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors? FAQ ID -1984 View Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits? FAQ ID -1964 View Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit? FAQ ID -1953 View Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits? FAQ ID -1960 View Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits? FAQ ID -1969 View Can I program my own protocols for the QIAsymphony SP? FAQ ID -1904 View Are QuantiFast Multiplex PCR Kits available in trial sizes? FAQ ID -1981 View Are there any specific cleaning solutions recommended for the QIAsymphony SP? FAQ ID -1913 View Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit? FAQ ID -1978 View Can the QIAsymphony RNA Kit be used to purify RNA from blood? FAQ ID -1945 View Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)? FAQ ID -1909 View Can protocol runs on the QIAsymphony SP be interrupted and resumed? FAQ ID -1912 View Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes? FAQ ID -1979 View Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples? FAQ ID -1995 View Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits? FAQ ID -1950 View Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits? FAQ ID -1951 View Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits? FAQ ID -1959 View Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits? FAQ ID -1975 View Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits? FAQ ID -1946 View Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits? FAQ ID -1947 View Do you have a protocol for the purification of DNA from fruit flies? FAQ ID -1970 View Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits? FAQ ID -1974 View How can the QIAsymphony SP system be decontaminated? FAQ ID -1914 View Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits? FAQ ID -1958 View Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits? FAQ ID -1955 View Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits? FAQ ID -1954 View Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits? FAQ ID -1967 View Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits? FAQ ID -1968 View Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits? FAQ ID -1948 View Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits? FAQ ID -1972 View Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits? FAQ ID -1973 View Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits? FAQ ID -1971 View Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits? FAQ ID -1965 View Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits? FAQ ID -1952 View What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits? FAQ ID -1751 View What is the fidelity of TopTaq DNA Polymerase? FAQ ID -1739 View Is TopTaq DNA Polymerase a hot-start polymerase? FAQ ID -1748 View Which nucleotide analogs can be used with TopTaq DNA Polymerase? FAQ ID -1741 View Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit? FAQ ID -1755 View What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits? FAQ ID -1738 View What is the shelf-life of TopTaq DNA Polymerase and Master Mix? FAQ ID -1743 View What is the principle of the REPLI-g FFPE Kit? FAQ ID -1753 View What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit? FAQ ID -1752 View What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit? FAQ ID -1750 View Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit? FAQ ID -1737 View What is the recommended sample size for use with the REPLI-g FFPE Kit? FAQ ID -1756 View Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit? FAQ ID -1749 View Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits? FAQ ID -1702 View Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits? FAQ ID -1740 View At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done? FAQ ID -1758 View Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex? FAQ ID -1714 View How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit? FAQ ID -1734 View Do you have protocols for sample processing on the Autopure LS instrument? FAQ ID -1759 View Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order? FAQ ID -1754 View Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples? FAQ ID -1760 View Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48? FAQ ID -1733 View Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications? FAQ ID -1744 View Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions? FAQ ID -1761 View Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? FAQ ID -1745 View Is FlexiTube GeneSolution available for other scales than the 1 nmol scale? FAQ ID -1666 View What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit? FAQ ID -1601 View What is the binding capacity of the Ni-NTA Superflow Cartridges? FAQ ID -1603 View Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit? FAQ ID -1610 View Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination? FAQ ID -1697 View Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification? FAQ ID -1609 View Is sequence information for HP Validated siRNAs provided in the FlexiTube format? FAQ ID -1665 View Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit? FAQ ID -1614 View Which siRNA controls are available in FlexiTube format? FAQ ID -1661 View Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits? FAQ ID -1690 View Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit? FAQ ID -1620 View What is the composition of Buffer PE? FAQ ID -1691 View What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1616 View Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series? FAQ ID -1606 View Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused? FAQ ID -1607 View Can I store cDNA generated by the miScript System? FAQ ID -1602 View Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples? FAQ ID -1612 View Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants? FAQ ID -1615 View Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase? FAQ ID -1617 View Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time? FAQ ID -1618 View Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs? FAQ ID -1664 View Can I still reorder siRNA that has been removed from the GeneGlobe data base? FAQ ID -1668 View Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields? FAQ ID -1608 View Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution? FAQ ID -1667 View Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube? FAQ ID -1658 View Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits? FAQ ID -1611 View How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red? FAQ ID -1644 View How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges? FAQ ID -1605 View How long is cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1613 View How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit? FAQ ID -1623 View Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure? FAQ ID -1619 View Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer? FAQ ID -1659 View Do you have a protocol for purification of total RNA from plant latex? FAQ ID -1694 View Do I need to use the miScript Universal Primer for detection of mRNA? FAQ ID -1600 View Do you offer a QuantiFast Kit for one-step RT-PCR? FAQ ID -1695 View Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit? FAQ ID -1693 View How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol? FAQ ID -2159 View How often can the Strep-Tactin Superflow Plus resin be reused? FAQ ID -2169 View If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit? FAQ ID -2129 View If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software? FAQ ID -2150 View Is it normal that the binding capacity of GST-resins decreases with flow rate? FAQ ID -2174 View Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow? FAQ ID -2172 View Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit? FAQ ID -2196 View Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits? FAQ ID -2118 View Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits? FAQ ID -2122 View Why do I need normalization using Rotor-Gene ScreenClust HRM Software? FAQ ID -2198 View Why is there an asterisk for my miScript Primer Assays? FAQ ID -2176 View Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers? FAQ ID -2121 View Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits? FAQ ID -2139 View Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC? FAQ ID -2124 View What is miRNA*? Do I need to consider this in my profiling experiment? FAQ ID -2179 View What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits? FAQ ID -2119 View What are possible reasons for reduced DNA yields with REPLI-g Kits? FAQ ID -2148 View What is the residuals plot in the Rotor-Gene ScreenClust HRM Software? FAQ ID -2199 View What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2158 View What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates? FAQ ID -2109 View What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits? FAQ ID -2144 View What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit? FAQ ID -2145 View How should DNA isolated on the QIAsymphony be stored? FAQ ID -2165 View How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2157 View How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be? FAQ ID -2193 View Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis? FAQ ID -2188 View Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow? FAQ ID -2173 View Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2160 View What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony? FAQ ID -2156 View Which plate formats are offered for QuantiTect Primer Assay Plates? FAQ ID -2106 View What species are QuantiTect Primer Assay Plates offered for? FAQ ID -2110 View Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits? FAQ ID -2147 View Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol? FAQ ID -2154 View How many reactions can be performed per well of the QuantiTect Primer Assay Plates? FAQ ID -2107 View Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types? FAQ ID -2190 View Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate? FAQ ID -2108 View Can Glutathione Superflow resin be reused? FAQ ID -2171 View Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use? FAQ ID -2138 View Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax? FAQ ID -2112 View Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix? FAQ ID -2168 View Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits? FAQ ID -2134 View Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2130 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit? FAQ ID -2131 View Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers? FAQ ID -2142 View Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit? FAQ ID -2135 View Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis? FAQ ID -2191 View Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument? FAQ ID -2116 View Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)? FAQ ID -2136 View Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online? FAQ ID -2111 View Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits? FAQ ID -2126 View How does template quality influence the results with the Type-it HRM PCR Kit? FAQ ID -2197 View How does magnetic-particle carry-over in QIAsymphony eluates need to be handled? FAQ ID -2166 View Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits? FAQ ID -2105 View How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments? FAQ ID -2189 View How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits? FAQ ID -2125 View How many elution steps are recommended when using Glutathione Superflow resin? FAQ ID -2175 View How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit? FAQ ID -2192 View Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits? FAQ ID -2104 View Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2163 View Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol? FAQ ID -2162 View Can Vapor-Lock be used with samples processed on the QIAgility? FAQ ID -2153 View Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits? FAQ ID -2123 View Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment? FAQ ID -2117 View Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit? FAQ ID -2128 View What main input voltage is required for the QIAxcel Analyzer? FAQ ID -1821 View What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced? FAQ ID -1852 View Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits? FAQ ID -1882 View The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence? FAQ ID -1850 View Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System? FAQ ID -1832 View What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly? FAQ ID -1834 View Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced?? FAQ ID -1847 View Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced? FAQ ID -1844 View Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on? FAQ ID -1851 View Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System? FAQ ID -1828 View Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used? FAQ ID -1841 View Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate? FAQ ID -1861 View How can a positive threshold be set when running the QIAxcel System? FAQ ID -1843 View How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System? FAQ ID -1827 View How long will the intensity calibration procedure on the QIAxcel System take? FAQ ID -1826 View Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed? FAQ ID -1824 View Will the QIAxcel System run using a green Data Review Software Key? FAQ ID -1853 View Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer? FAQ ID -1848 View Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced? FAQ ID -1835 View When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider? FAQ ID -1842 View What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced? FAQ ID -1833 View Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced? FAQ ID -1837 View When should an intensity calibration be re-performed on the QIAxcel System? FAQ ID -1825 View When should the N2 bottle used with the QIAxcel System be changed? FAQ ID -1830 View Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System? FAQ ID -1855 View Can the QIAxcel System or QIAxcel Advanced be connected to a network? FAQ ID -1862 View How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced? FAQ ID -1836 View Can multiple QIAxcel Systems be run from one computer? FAQ ID -1860 View If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated? FAQ ID -1858 View If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created? FAQ ID -1857 View How often should the alignment marker for the QIAxcel System be changed? FAQ ID -1839 View How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced? FAQ ID -1840 View In the results table generated with the QIAxcel System, what does "normalized area" mean? FAQ ID -1854 View How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented? FAQ ID -1823 View If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created? FAQ ID -1856 View How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced? FAQ ID -1838 View What is the composition of buffer QBT? FAQ ID -411 View What is the composition of buffer QC? FAQ ID -412 View What is the composition of buffer P3? FAQ ID -418 View What is the composition of buffer QF? FAQ ID -413 View What is the composition of buffer TE? FAQ ID -416 View What is the composition of buffer QN? FAQ ID -414 View What is the composition of buffer OCD? FAQ ID -423 View What is the composition of buffer FWB2? FAQ ID -417 View What is the composition of buffer OBB? FAQ ID -421 View What is the composition of buffer OW2? FAQ ID -419 View What is the composition of buffer OL2? FAQ ID -424 View What is the composition of buffer OCL? FAQ ID -422 View Can RNAi experiments be performed in Drosophila? FAQ ID -436 View What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors? FAQ ID -470 View Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant? FAQ ID -472 View Has RNAi been successful using siRNA in Zebrafish and Xenopus? FAQ ID -400 View How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit? FAQ ID -468 View How do I calculate the percentage of silencing with real-time RT-PCR for siRNA? FAQ ID -498 View Are Northern Blots sensitive enough to detect siRNA-induced gene silencing? FAQ ID -403 View Can QIAquick Kits be used to clean up RNA samples? FAQ ID -490 View Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit? FAQ ID -482 View Can Ni-NTA resins be used to purify protein with an internal His-tag? FAQ ID -496 View What is the composition of buffer STE? FAQ ID -415 View Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction? FAQ ID -429 View What is the composition of buffer OEB? FAQ ID -420 View Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed? FAQ ID -474 View Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues? FAQ ID -467 View Why is the pQE DNA provided in QIAexpress Kits blue in color? FAQ ID -487 View Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? FAQ ID -473 View What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit? FAQ ID -432 View What is the minimum elution volume when using the RNeasy Micro Kit? FAQ ID -428 View What is the smallest sample size that can be used with RNeasy Mini Kits? FAQ ID -485 View What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit? FAQ ID -426 View What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System? FAQ ID -480 View What is a homogeneous LiquiChip assay? FAQ ID -476 View What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument? FAQ ID -479 View What is the binding capacity of the RNeasy Micro Kit column? FAQ ID -427 View What is the composition and size of the LiquiChip Beads? FAQ ID -475 View What is the binding capacity of the RNeasy MinElute Cleanup Kit column? FAQ ID -430 View Is dihydrofolate reductase (DHFR) immunogenic? FAQ ID -471 View What are some of the assays that are adaptable to the LiquiChip System? FAQ ID -477 View My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use? -20 View Will REPLI-g work at high temperatures? FAQ ID -656 View What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument? FAQ ID -622 View The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do? FAQ ID -652 View What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction? FAQ ID -663 View What is the shelf-life of my QIAGEN Kit? FAQ ID -651 View What is the recommended incubation time to stabilize tissue RNA in RNAprotect? FAQ ID -647 View What do you mean by “Quantification of locus representation” in REPLI-g WGA product? FAQ ID -698 View What is the average amount of DNA and RNA present in 1 ml normal serum? FAQ ID -635 View What is REPLI-g whole genome amplification? FAQ ID -654 View What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample? FAQ ID -631 View What do you recommend for the cleanup of genomic DNA (gDNA)? FAQ ID -618 View What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? FAQ ID -632 View What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System? FAQ ID -601 View What is the length of the MDA whole genome amplification product? FAQ ID -690 View What 96-well plates do you recommend for use with the LiquiChip Workstation? FAQ ID -630 View Is it possible to perform laser microdissection with RNAprotect-stabilized tissue? FAQ ID -608 View Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit? FAQ ID -602 View What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them? FAQ ID -646 View What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification? FAQ ID -665 View What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods? FAQ ID -686 View Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA? FAQ ID -675 View Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? FAQ ID -633 View Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit? FAQ ID -603 View Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit? FAQ ID -604 View Why do you recommend using Triton X for the purification of 6xHis-tagged protein? -100 View Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine? FAQ ID -644 View Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing? FAQ ID -637 View Will TA cloning work with REPLI-g WGA product? FAQ ID -672 View Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification? FAQ ID -664 View What is the stability of the REPLI-g MDA product? FAQ ID -693 View When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong? FAQ ID -653 View Where should I add a dye-label modification on the siRNA? FAQ ID -648 View What types of materials from environmental samples could interfere with WGA? FAQ ID -666 View What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit? FAQ ID -600 View Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent? FAQ ID -615 View Can I run less than 15 samples on the BioSprint 15 instrument? FAQ ID -621 View Can I re-amplify whole genome amplification product? FAQ ID -670 View Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit? FAQ ID -645 View Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples? FAQ ID -616 View Do you have an off-the shelf diagnostics kit for WGA? FAQ ID -678 View Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution? FAQ ID -619 View Can the computer for the LiquiChip Workstation be networked? FAQ ID -629 View Are there components in biological samples that can inhibit the REPLI-g reaction? FAQ ID -667 View Are the BioSprint 15/96 instruments stand-alone workstations? FAQ ID -620 View Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification? FAQ ID -683 View Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-? FAQ ID -638 View Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing? FAQ ID -643 View Can I do whole genome amplification from brain tissue using REPLI-g? FAQ ID -669 View Can FTA paper for dried blood spots be used with REPLI-g? FAQ ID -685 View Can I do whole genome amplification from mitochondrial DNA using REPLI-g? FAQ ID -668 View Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits? FAQ ID -671 View How can I minimize degradation of RNA from my pancreas sample? FAQ ID -624 View How can I quantify the amount of REPLI-g DNA I have amplified? FAQ ID -694 View How should I store the REPLI-g amplification product? FAQ ID -692 View Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification? FAQ ID -682 View How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent? FAQ ID -614 View Do I need to clean up the REPLI-g amplification product for downstream analysis? FAQ ID -691 View Does REPLI-g technology for whole genome amplification work with paraffin embedded samples? FAQ ID -673 View Do you have a kit for RNA isolation from any kind of sample type? FAQ ID -627 View How can I improve my RNA yield from liver sample when using RNeasy Kits? FAQ ID -623 View How can I determine the quality of my REPLI-g amplified products? FAQ ID -695 View Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA? FAQ ID -684 View What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits? FAQ ID -606 View Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit? FAQ ID -599 View I want to start with gene silencing (RNAi) experiments. Do you have informational literature? FAQ ID -580 View What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried? FAQ ID -572 View What is a quenched FRET assay? FAQ ID -542 View Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1? FAQ ID -573 View What do you recommend for isolating RNA from more than 100 mg of plant tissue? FAQ ID -570 View What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed? FAQ ID -514 View What has to be done to a RNA sample before loading it onto an Agilent Bioanalyzer? FAQ ID -528 View How should I dissolve the lyophilized Anti-His and Tag Antibodies? -100 View Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels? FAQ ID -576 View Is it possible to perform a UNG treatment when using QuantiTect kits? FAQ ID -564 View Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits? FAQ ID -563 View Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits? FAQ ID -519 View Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? FAQ ID -532 View Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits? FAQ ID -533 View Why are my realtime PCR amplification plots hook-shaped? FAQ ID -587 View How was it determined that the PhosphoSerine Antibody will bind phosphoserines only? FAQ ID -579 View If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards? FAQ ID -513 View How should I store cDNA produced using Omniscript Reverse Transcriptase? FAQ ID -561 View Which chloroform should I use for the RNeasy Lipid Tissue Kit? FAQ ID -516 View Will pancreas tissue swim on top of RNAprotect? FAQ ID -536 View Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C? FAQ ID - 525 View Why does my realtime PCR assay quality decrease over time? FAQ ID -589 View Why do replicates in real-time PCR have different plateau heights? FAQ ID -539 View What is the longest fragment that was cloned with the pQE UA Cloning Vector? -30 View What is the difference between BSA-free and BSA-containing RGS-His Antibody? FAQ ID -569 View What is the composition of Buffer ER? Is it available separately? FAQ ID -571 View What is the composition of the siRNA resuspension & annealing buffer? FAQ ID -522 View When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK? FAQ ID -534 View Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page? FAQ ID -562 View What reaction volume should I use with QuantiTect Kits? FAQ ID -551 View What is Tissue-Tek O.C.T., and what is it used for? FAQ ID -530 View What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior? FAQ ID -566 View How many template copies can I detect using QuantiTect Kits in real-time PCR? FAQ ID -543 View Can I use RNAiFect for co-transfection of plasmid DNA and siRNA? FAQ ID -515 View Does DEPC harm RNA? FAQ ID -529 View Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture? FAQ ID -527 View Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls? FAQ ID -523 View Can the MAPK1 control siRNA be used for rat cells? FAQ ID -547 View Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical? FAQ ID -581 View Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? FAQ ID -577 View After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that? FAQ ID -512 View Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use? FAQ ID -584 View Can I shorten the activation time for the HotStarTaq DNA Polymerase? FAQ ID -565 View Can I homogenize samples in QIAzol using the QIAshredder? FAQ ID -501 View Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative? FAQ ID -560 View How can I avoid primer-dimer formation during PCR amplification? FAQ ID -544 View Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder? FAQ ID -594 View Do you provide control proteins for use with the PhosphoProtein Purification Kit? FAQ ID -596 View How long should the amplicon be if I am using QuantiTect SYBR Green detection? FAQ ID -553 View How is an antibody epitope determined? FAQ ID -593 View Do I need to calibrate my real-time cycler if I want to use Yakima Yellow? FAQ ID -540 View How can I tell if I have primer-dimers in my PCR reaction? FAQ ID -552 View Do we recommend pooling siRNA sequences? FAQ ID -517 View Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit? FAQ ID -575 View Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit? FAQ ID -598 View Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture? FAQ ID -548 View Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1? FAQ ID -567 View Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit? FAQ ID -518 View Do you have a protocol for the removal of endotoxins from already purified plasmid DNA? FAQ ID -500 View Do you have a protocol for isolating RNA from yeast using RNeasy? FAQ ID -535 View Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells? FAQ ID -597 View Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit? FAQ ID -931 View Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells? FAQ ID -926 View Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System? FAQ ID -979 View Do you have a protocol for the isolation of DNA from tofu? FAQ ID -932 View Do you have a protocol for Streptokinase treatment of clotted blood? FAQ ID -912 View Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit? FAQ ID -973 View Do you have a protocol for the isolation of RNA from leukocytes in milk? FAQ ID -952 View Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1? FAQ ID -975 View Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1? FAQ ID -974 View Do you have a protocol for the isolation of total RNA from buccal swabs? FAQ ID -956 View Do you have a protocol for the isolation of viral DNA from stool? FAQ ID -929 View Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips? FAQ ID -949 View Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1? FAQ ID -976 View Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit? FAQ ID -951 View Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser? FAQ ID -924 View Do you have a protocol for the isolation of genomic DNA from sperm? FAQ ID -909 View Do you have a protocol for the isolation of DNA from formalin-preserved stool samples? FAQ ID -922 View Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1? FAQ ID -992 View Do you have a protocol for the isolation of genomic DNA from fungi? FAQ ID -911 View Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol? FAQ ID -955 View Do you have a protocol for the isolation of genomic DNA from frozen clotted blood? FAQ ID -900. Test View Do you have a protocol for the preparation of RNAprotect preserved tissues for pathological studies? FAQ ID -950 View Do you have a protocol for the purification of DNA from cells using the BioSprint System? FAQ ID -977 View Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System? FAQ ID -982 View Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips? FAQ ID -902 View Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent? FAQ ID -970 View Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System? FAQ ID -983 View Do you have a protocol for GMO testing of food samples? FAQ ID -921 View Do you have a protocol for isolation of bacterial DNA from soil? FAQ ID -923 View Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips? FAQ ID -901 View Do you have a protocol for isolation of genomic DNA from saliva and mouthwash? FAQ ID -917 View Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit? FAQ ID -919 View Do you have a protocol for isolation of genomic DNA from insects? FAQ ID -904 View Do you have a protocol for detection of HBV DNA by PCR? FAQ ID -915 View Can the BioSprint instruments be placed in a cool room? FAQ ID -938 View Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit? FAQ ID -937 View Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples? FAQ ID -913 View Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA? FAQ ID -969 View Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA? FAQ ID -959 View Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System? FAQ ID -965 View Do you have a protocol for cleanup of non-radioactive DNA samples? FAQ ID -945 View Do you have a protocol for radioactive labelling of cDNA? FAQ ID -960 View Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates? FAQ ID -971 View Do you have a protocol for the isolation of genomic DNA from bone? FAQ ID -908 View Do you have a protocol for the isolation of viral RNA from stool? FAQ ID -918 View Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)? FAQ ID -907 View Do you have protocols for multiple extractions of DNA fragments from agarose gels? FAQ ID -944 View Why is it not recommended to stabilize cells with RNAprotect? FAQ ID -941 View What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy? FAQ ID -942 View Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent? FAQ ID -997 View Do you have a protocol for the detection of Bordetella pertussis DNA by PCR? FAQ ID -914 View Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1? FAQ ID -985 View Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1? FAQ ID -990 View Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system? FAQ ID -966 View Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent? FAQ ID -972 View Do you have a protocol for polyacrylamide gel analysis of oligonucleotides? FAQ ID -961 View Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow? FAQ ID -967 View Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit? FAQ ID -934 View Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions? FAQ ID -947 View Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit? FAQ ID -935 View Do you have a protocol for the purification of PCR products using the BioSprint System? FAQ ID -984 View Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips? FAQ ID -905 View Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips? FAQ ID -903 View Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2? FAQ ID -397 View Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit? FAQ ID -358 View Is QIAGEN carrier RNA sold separately? FAQ ID -351 View I accidentally added RNAprotect to my cells, and now the cells are difficult to pellet. What can I do? FAQ ID -364 View What are the calculated molecular weights of the TAGZyme enzymes? FAQ ID -329 View Is it possible to store TAGzyme enzymes at°C? -80 View Is Q-Solution required for PCR with QIAGEN's PCR kits? FAQ ID -380 View How do I know if my plasmid is a high- or low copy number type? FAQ ID -350 View How do I perform a DNA precipitation to concentrate my sample? FAQ ID -305 View How can QIAGEN Protease and Proteinase K be inactivated? FAQ ID -315 View How complete is the removal of DAPase in the TAGZyme process? FAQ ID -319 View How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs? FAQ ID -392 View How many transfections can I perform with 20 nmol of HPP grade siRNA? FAQ ID -367 View How do I submit a siRNA order by telephone or online? FAQ ID -399 View What is the white insoluble precipitate in my resuspended plasmid DNA pellet? FAQ ID -352 View What kits does QIAGEN offer to extract RNA from whole blood? FAQ ID -304 View Why do I get genomic DNA contamination in my plasmid prep? FAQ ID -353 View Which primers can I use for sequencing pQE-expression vector constructs? FAQ ID -343 View What is the standard assay volume recommended for LiquiChip assays? FAQ ID -330 View What is the average molecular weight of a siRNA, and how do I convert uM to ug values? FAQ ID -388 View What are the dissociation constants for the Anti-His Antibodies? FAQ ID -385 View What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules? FAQ ID -307 View What is the origin of replication and the plasmid copy number of the pQE vectors? FAQ ID -338 View What is the acceptable maximum g force for centrifugation of Xa Removal Resin? FAQ ID -320 View What is the composition of Buffer FG3? FAQ ID -356 View What is the composition of PBS? FAQ ID -361 View How can I keep my centrifuge tubes endotoxin-free? FAQ ID -301 View Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits? FAQ ID -354 View 303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? View Can I store agarose gel slices containing DNA for gel extraction at a later point? FAQ ID -313 View Can I use a partial MinElute 96 UF plate? FAQ ID -355 View Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells? FAQ ID -372 View Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits? FAQ ID -306 View Are QIAprep and QIAquick Spin columns interchangeable? FAQ ID -311 View Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable? FAQ ID -308 View Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein? FAQ ID -359 View Are QIAGEN monoclonal Anti His Antibodies glycosylated? FAQ ID -334 View Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription? FAQ ID -366 View Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit? FAQ ID -332 View Can Buffers N3 and P3 be used interchangeably? FAQ ID -310 View Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR? FAQ ID -328 View How are siRNAs introduced into C. elegans? FAQ ID -396 View Do I need to anneal, deprotect or desalt my QIAGEN siRNA? FAQ ID -398 View Do pQE vectors contain the CAT gene? FAQ ID -362 View How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit? FAQ ID -374 View How can I improve the expression of proteins containing hydrophobic regions? FAQ ID -339 View How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution? FAQ ID -324 View How can I express toxic protein in E. coli? FAQ ID -373 View Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector? FAQ ID -323 View Can RNAprotect Tissue Reagent be used to store previously isolated RNA? FAQ ID -337 View Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil? FAQ ID -360 View Can siRNA silence bacterial genes? FAQ ID -395 View Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining? FAQ ID -312 View Does the 5x OneStep RT-PCR Buffer contain BSA? FAQ ID -326 View Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton? FAQ ID -327 View Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex? FAQ ID -318 View What are exo-resistant random hexamers used in the REPLI-g reaction? FAQ ID -710 View The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem? FAQ ID -776 View Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit? FAQ ID -797 View What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit? FAQ ID -721 View What are the expected average DNA yields from plant extractions on the BioSprint 96? FAQ ID -771 View What is the basic technology behind the Strep-tag Protein Purification System? FAQ ID -740 View What is the advantage of running an analytical gel with fractions of my plasmid preparation? FAQ ID -769 View What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728 View I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost? FAQ ID -756 View If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits? FAQ ID -718 View How to quantitate DNA purified using the BioSprint 96? FAQ ID -773 View How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead? FAQ ID -734 View I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20 View Is it possible to use LiquiChip beads for FACS applications? FAQ ID -733 View Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA? FAQ ID -785 View I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745 View Is GFP excited and detected by the green laser of the LiquiChip System? FAQ ID -737 View Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately? FAQ ID -763 View Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples? FAQ ID -755 View Why are some of my RNAprotect-stabilized samples frozen atºC while others are not? ID -758 View Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits? FAQ ID -719 View Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers? FAQ ID -715 View Why might my gene of interest drop out during WGA if it is not near a telomere or centromere? FAQ ID -703 View Will the random hexamers in the REPLI-g reaction interfere with downstream analysis? FAQ ID -713 View Why should DNA or cDNA targets be less than 250 bp long for real-time PCR? FAQ ID-751 View Why is my plasmid DNA yield low? FAQ ID -768 View What is the difference between Ni-NTA Agarose and Ni-NTA Superflow? FAQ ID -764 View What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? FAQ ID -761 View Why is there no detection of free reporter fluorescence in the LiquiChip System? FAQ ID -732 View What is the composition of buffer AE? FAQ ID -730 View What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR? FAQ ID -782 View What is the composition of Buffer N3? FAQ ID -767 View What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit? FAQ ID -779 View Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604? FAQ ID -766 View What type of Sequencing Services does QIAGEN offer? FAQ ID -781 View What is the enzyme used in the REPLI-g reaction? FAQ ID -704 View What is the recipe for SOC medium? FAQ ID -798 View What is the general sensitivity level of the LiquiChip System? FAQ ID -731 View How should fluorescent labeled probes be stored? FAQ ID -784 View Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing? FAQ ID -741 View Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen? FAQ ID -757 View Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size? FAQ ID -778 View Can lyophilized plant material be used for DNA extraction on the BioSprint 96? FAQ ID -772 View Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays? FAQ ID -717 View Can the REPLI-g WGA enzyme work with labeled dNTPs? FAQ ID -706 View Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately? FAQ ID -744 View Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells? FAQ ID -795 View Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates? FAQ ID -722 View Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately? FAQ ID -791 View Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa? FAQ ID -770 View Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable? FAQ ID -786 View Any data on the fidelity of the REPLI-g MDA technique? FAQ ID -707 View Are Centromeres and Telomeres amplified using REPLI-g WGA? FAQ ID -702 View Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region? FAQ ID -712 View Can I use REPLI-g for SNP Genotyping? FAQ ID -700 View Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit? FAQ ID -716 View Can I purchase Phi29 DNA polymerase only? FAQ ID -708 View How can I get specific information and a quote for my individual Sequencing Service needs? FAQ ID -792 View How can one perform multiplex real-time PCR analysis using the LightCycler 2.0? FAQ ID -780 View How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression? FAQ ID -788 View How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN? FAQ ID -762 View How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination? FAQ ID -783 View How many cells can be used with the FastLane Cell cDNA Kit? FAQ ID -796 View Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA? FAQ ID -746 View Do I have to switch off the laser of the LiquiChip Workstation overnight? FAQ ID -736 View Can frozen blood be used for whole genome amplification with REPLI-g? FAQ ID -794 View How many reporter dyes can be used in the same analysis using the LiquiChip Workstation? FAQ ID -738 View How much DNA is obtained in the average PCR reaction? FAQ ID -750 View Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits? FAQ ID -714 View Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA? FAQ ID -754 View Do you synthesize thiophosphate modified random hexamers? FAQ ID -711 View Has anyone verified whole genome amplification accuracy with Sequencing? FAQ ID -701 View How best to handle the light sensitive LiquiChip reagents? FAQ ID -735 View Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA? FAQ ID -793 View Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns? FAQ ID -759 View Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR? FAQ ID -729 View Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48? FAQ ID -1019 View Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation? FAQ ID -1040 View How can I prevent running out of M48 Kit reagents before the full number of samples are processed? FAQ ID -1042 View What are the additional plasmid bands I see on my gel? FAQ ID -1059 View Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA? FAQ ID -1063 View Is it possible to use the PhosphoProtein Purification Kit in batch format? FAQ ID -1069 View What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components? FAQ ID -1077 View What is the difference between Buffers RLT and RLT Plus? FAQ ID -1043 View What is the difference between TurboCapture Microplate Holders Type A and Type B? FAQ ID -1038 View Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits? FAQ ID -1071 View What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies? FAQ ID -1093 View Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit? FAQ ID -1049 View How can I transfect siRNA into insect cells such as Drosophila melanogaster S2? FAQ ID -1046 View How can I tell TurboCapture Microplate Holder Type A from Type B? FAQ ID -1039 View How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits? FAQ ID -1086 View I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that? FAQ ID -1045 View How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent? FAQ ID -1058 View What is the error rate of HotStar HiFidelity DNA Polymerase? FAQ ID -1048 View How do I perform an Acetone Precipitation for concentrating and desalting protein samples? FAQ ID -1035 View Which kit should I use for RNA isolation from Cartilage? FAQ ID -1026 View Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells? FAQ ID -1092 View Where can I find the serial number on QIAGEN's BioRobots? FAQ ID -1064 View Which kit can be used for simultaneous purification of DNA and RNA from biopsies? FAQ ID -1089 View Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature? FAQ ID -1083 View Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column? FAQ ID -1044 View Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit? FAQ ID -1054 View Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase? FAQ ID -1053 View Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits? FAQ ID -1084 View Where can I find a protocol for cleanup of already purified plasmid DNA? FAQ ID -1031 View What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit? FAQ ID -1090 View What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit? FAQ ID -1047 View What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve? FAQ ID -1079 View What is the recipe for 1x PBS solution? FAQ ID -1030 View What should I use as a standard for absolute quantification in real-time PCR? FAQ ID -1085 View What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)? FAQ ID -1055 View What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits? FAQ ID -1034 View What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit? FAQ ID -1067 View How can I prevent clogging of QIAfilter cartridges? FAQ ID -1060 View Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage? FAQ ID -1037 View Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays? FAQ ID -1065 View Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase? FAQ ID -1050 View Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase? FAQ ID -1052 View Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent? FAQ ID -1020 View Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48? FAQ ID -1017 View Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument? FAQ ID -1032 View Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96? FAQ ID -1007 View Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene? FAQ ID -1091 View Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel? FAQ ID -1051 View Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)? FAQ ID -1025 View Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit? FAQ ID -1078 View Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells? FAQ ID -1036 View Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably? FAQ ID -1066 View Can the AllPrep RNA/Protein Kit be used with tissue? FAQ ID -1095 View Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies? FAQ ID -1073 View Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis? FAQ ID -1056 View How can I check for purity of RNA isolated using RNeasy Kits? FAQ ID -1023 View How can air bubbles in PhosphoProtein Purification Columns be avoided? FAQ ID -1070 View How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits? FAQ ID -1062 View How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits? FAQ ID -1061 View How can I check the integrity of RNA purified using RNeasy Kits? FAQ ID -1024 View How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set? FAQ ID -1087 View Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot? FAQ ID - 1004 View Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect? FAQ ID -1012 View Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks? FAQ ID -1014 View Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent? FAQ ID -1013 View Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot? FAQ ID -1009 View Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot? FAQ ID -1008 View Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits? FAQ ID -1011 View Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot? FAQ ID -1010 View Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot? FAQ ID - 1003 View Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot? FAQ ID -1006 View What Disinfectant Solution do you recommend for the BioRobot MDx? FAQ ID -809 View What data documentation does QIAGEN's Sequencing Service provide for customers? FAQ ID -832 View What are the sequences of the FlexiTube siRNAs? FAQ ID -851 View What annealing temperature should be used with the QuantiTect Primer Assays? FAQ ID -849 View What is the proper way to turn on or turn off the EZ1 instrument? FAQ ID -870 View My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols? FAQ ID -872 View What is the Energy Mix in the EasyXpress Kits? FAQ ID -846 View What is the concentration of the primers in a reaction using the QuantiTect Primer Assays? FAQ ID -850 View I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA? FAQ ID -829 View Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit? FAQ ID -855 View Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit? FAQ ID -835 View Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate? FAQ ID -854 View Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit? FAQ ID -841 View Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit? FAQ ID -876 View Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit? FAQ ID -837 View Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity? FAQ ID -875 View Is the E. coli Host Strain M15[pREP4] resistant to Zeocin? FAQ ID -842 View Will acetone precipitation recommended in the Qproteome Protocols denature Protein? FAQ ID -807 View Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit? FAQ ID -878 View Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation? FAQ ID -862 View Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits? FAQ ID -860 View With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns? FAQ ID -864 View Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein? FAQ ID -877 View Will the sequences of the QuantiTect Primer Assays be provided? FAQ ID -804 View Which resin is used in the QIAexpress Ni-NTA Fast Start Columns? FAQ ID -836 View What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent? FAQ ID -861 View Will the FastLane Cell cDNA Kit work with suspension cells? FAQ ID -801 View What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in? FAQ ID -815 View What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit? FAQ ID -873 View What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment? FAQ ID -831 View Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx? FAQ ID -806 View Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent? FAQ ID -865 View Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit? FAQ ID -839 View Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit? FAQ ID -808 View Do I need to use RNase inhibitors with the RNeasy Kits? FAQ ID -813 View Where can I find QIAGEN products for a specific gene or gene product? FAQ ID -803 View Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent? FAQ ID -824 View Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR? FAQ ID -834 View Can the QuantiTect Primer Assays be used for detection of genomic DNA? FAQ ID -811 View Can the FastLane Cell cDNA Kit be used with tissue? FAQ ID -821 View Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits? FAQ ID -883 View Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis? FAQ ID -879 View 895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit? View Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4? -15 View Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit? FAQ ID -881 View Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins? FAQ ID -802 View Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1? FAQ ID -823 View Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples? FAQ ID -828 View Can I stabilize adipose tissue using RNAprotect prior to RNA isolation? FAQ ID -856 View Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast? FAQ ID -838 View Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits? FAQ ID -874 View Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems? FAQ ID -863 View Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit? FAQ ID -812 View Have you tested the effect of inhibitors on PCR performance? FAQ ID -818 View Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately? FAQ ID -825 View How can I separate PCR fragments that are small and very close in size on an agarose gel? FAQ ID -800 View Do you have information on in vivo RNA interference experiments? FAQ ID -817 View I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast? FAQ ID -822 View I left Buffer P1 at room temperature after addition of RNase A, what shall I do? FAQ ID -859 View How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits? FAQ ID -880 View How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit? FAQ ID -843 View Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.? FAQ ID -893 View Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii? FAQ ID -885 View Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum? FAQ ID -886 View Do you have a protocol for the isolation of plasmid DNA from Agrobacterium? FAQ ID -898 View Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.? FAQ ID -884 View Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis? FAQ ID -896 View Do you have a protocol for the isolation of plasmid DNA from yeast? FAQ ID -853 View Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.? FAQ ID -887 View Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits? FAQ ID -894 View Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.? FAQ ID -890 View Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans? FAQ ID -888 View Do you have a protocol for the isolation of plasmid DNA from Proteus spp.? FAQ ID -889 View What are the most commonly used protease inhibitors? FAQ ID -53 View How should I quantify RNA isolated with RNeasy Kits? FAQ ID -32 View Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription? FAQ ID -1 View How should I propagate pQE expression plasmids? FAQ ID -58 View What are the compatibilities of different reagents with Ni-NTA matrices? FAQ ID -49 View How is "Touchdown PCR" used to increase PCR specificity? FAQ ID -75 View What should the starting template DNA quality and quantity be for PCR? FAQ ID -74 View Why do I get smeared PCR products? FAQ ID -87 View Why does my isolated RNA have a low OD 260/280 ratio? FAQ ID -97 View When is chloramphenicol amplification of plasmids performed? FAQ ID -3 View How can I remove imidazole from a protein sample? FAQ ID -91 View How can I increase the amount of soluble recombinant protein in E. coli expression? FAQ ID -64 View How can I avoid little or no RNA yields when using an RNeasy Kit? FAQ ID -28 View What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit? FAQ ID -160 View What are the molecular weights of proteins in the 6xHis Protein Ladder? FAQ ID -169 View What are your recommendations for cotransfecting several plasmids? FAQ ID -124 View What is the composition of Buffer EB? FAQ ID -199 View Is mRNA isolation necessary for sensitive RT-PCR? FAQ ID -111 View What are the recommended culture and buffer volumes for a very low-copy plasmid? FAQ ID -168 View Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins? FAQ ID -147 View What is the difference between disruption and homogenization in the RNeasy System? FAQ ID -139 View Which Anti-His Antibody is the most sensitive for my protein of interest? FAQ ID -172 View When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit? FAQ ID -100 View Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits? FAQ ID -101 View What is the recommended culture medium for the QIAprep System? FAQ ID -154 View What is the RNase A concentration and composition of Buffer P1? FAQ ID -198 View What is the genotype of the EZ Competent Cells? FAQ ID -157 View What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit? FAQ ID -165 View What is the principle behind Effectene Transfection Reagent? FAQ ID -184 View What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips? FAQ ID -142 View How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification? FAQ ID -102 View How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology? FAQ ID -134 View How can I improve recoveries when using the QIAquick Kits? FAQ ID -180 View How can I extract DNA from a polyacrylamide (PAGE) gel? FAQ ID -120 View How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit? FAQ ID -141 View How can I isolate RNA from 1 gram of plant tissue? FAQ ID -128 View How can I improve the performance of the HiSpeed QIAprecipitator module? FAQ ID -144 View How can I improve transfection efficiency using Effectene Transfection Reagent? FAQ ID -181 View What can I use to isolate RNA smaller than 200 nucleotides? FAQ ID -115 View What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize? FAQ ID -170 View What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit? FAQ ID -167 View How should RNeasy Kits be stored and how long are they stable? FAQ ID -103 View Is it possible to cleave the 6xHis-tag from an expressed protein? FAQ ID -140 View How should I store plant material for DNA isolation using the DNeasy Plant Kit? FAQ ID -114 View How should QIAGEN Plasmid Purification Kits be stored and for how long? FAQ ID -192 View What are the features and benefits of the QIAexpress 6xHis Tag System? FAQ ID -193 View What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick? FAQ ID -148 View Do you have transfection data for QIAGEN Transfection Reagents? FAQ ID -158 View Do I need to use an RNase inhibitor in my RT reaction? FAQ ID -119 View Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit? FAQ ID -129 View How can I avoid variable transfection efficiencies with SuperFect Reagent? FAQ ID -179 View Does QIAGEN offer vectors for direct cloning of PCR products? FAQ ID -146 View Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel? FAQ ID -133 View Are QIAamp DNA isolation kits suitable for apoptosis studies? FAQ ID -149 View Can DyeEx be used to clean up labeled DNA other than sequencing templates? FAQ ID -137 View Are RNeasy spin columns sold separately? FAQ ID -159 View Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? FAQ ID -127 View Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup? FAQ ID -130 View Can I use a QIAfilter Cartridge for purifying large plasmids? FAQ ID -121 View Can I use Omniscript or Sensiscript RT's at a higher temperature? FAQ ID -116 View How does imidazole affect my quantitation of protein? FAQ ID -132 View How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent? FAQ ID -176 View How do you ensure that RNeasy buffers are RNase-free? FAQ ID -113 View How can I purify DNA from soil, food and sewage samples for PCR? FAQ ID -118 View How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit? FAQ ID -143 View How do I prepare an insert for pQE vectors? FAQ ID -185 View How should I store Anti·His and Tag·100 Antibodies? FAQ ID -194 View Do you have a protocol for purification of total DNA from insects? FAQ ID -1254 View Is your Nuclease-Free Water fluorescence-free? FAQ ID -1291 View Do you have a protocol for purification of total DNA from crude lysates? FAQ ID -1255 View How should the GelPilot DNA Loading Dye be handled? FAQ ID -1287 View What quality level does your Nuclease-Free Water have? FAQ ID -1292 View What is the pH value of QIAGEN's Nuclease-Free Water? FAQ ID -1290 View Does GelPilot DNA Loading Dye contain ethidium bromide? FAQ ID -1286 View Are MaXtract tubes siliconized? FAQ ID -1298 View Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time? FAQ ID -1299 View Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples? FAQ ID -1211 View What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit? FAQ ID -1205 View What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit? FAQ ID -1220 View What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II? FAQ ID -1221 View Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1? FAQ ID -1232 View Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit? FAQ ID -1213 View How should I prepare buffy coat samples for use on the BioRobot EZ1? FAQ ID -1249 View Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II? FAQ ID -1225 View Is training of lab personnel included with the purchase of a BioRobot EZ1? FAQ ID -1239 View Where can I find information about the worktable setup on the BioRobot EZ1? FAQ ID -1243 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care

What kind of automation can be done with the EasyXtal 15-well Tool?

FAQ ID -2220

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What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?

FAQ ID -2216

View

What dyes are used in the KRAS and EGFR PCR kits?

FAQ ID -2288

View

What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?

FAQ ID -2298

View

What format are the EasyXtal 15-well tools?

FAQ ID -2219

View

What do I need to provide when ordering a miScript Target Protector?

FAQ ID -2251

View

What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?

FAQ ID -2253

View

What is a miScript Precursor Assay?

FAQ ID -2214

View

What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2203

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What is a miScript Target Protector?

FAQ ID -2249

View

Which transfection reagent is recommended for transfection of the miScript Target Protectors?

FAQ ID -2252

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Which variants of the EasyXtal 15-well tool are available?

FAQ ID -2221

View

Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2205

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Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2204

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Why does the SeqTarget Prep Protocol require a 2-step procedure?

FAQ ID -2238

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Why does the miScript Target Protector not induce the degradation process?

FAQ ID -2263

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Why do I have wavy DNA bands on my agarose gel?

FAQ ID -2260

View

What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?

FAQ ID -2242

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When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?

FAQ ID -2294

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What type of samples can I use for the KRAS PCR and EGFR kits?

FAQ ID -2293

View

What voltage should be used to run my agarose gel with the GelPilot Agarose?

FAQ ID -2261

View

Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?

FAQ ID -2202

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Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?

FAQ ID -2267

View

Does the miScript Target Protector match the target sequence?

FAQ ID -2250

View

Do the KRAS and EGFR PCR kits contain a passive reference dye?

FAQ ID -2295

View

Do I have to order at least 4 FlexiTube siRNA Premixes?

FAQ ID -2266

View

How do I store my FlexiTube siRNA Premix?

FAQ ID -2270

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How is ARMS technology superior to alternative methods such as dye terminator sequencing?

FAQ ID -2290

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Effects of low A260/A230 ratios in RNA preparations on downstream applications

FAQ ID -2248

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How can I check the success of the normalization procedure for the SeqTarget System?

FAQ ID -2245

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How do I search for all the miScript Target Protectors for one species?

FAQ ID -2255

View

Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?

FAQ ID -2240

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Can PCR fragments be normalized for the SeqTarget System without a previous purification step?

FAQ ID -2246

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Can I use a 384 well plate for the KRAS and EGFR PCR kits?

FAQ ID -2296

View

Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?

FAQ ID -2234

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Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?

FAQ ID -2211

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Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?

FAQ ID -2244

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What are EasyXtal 15-well Tools used for?

FAQ ID -2217

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What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?

FAQ ID -2200

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Is the SeqTarget System compatible with standard-size PCRs?

FAQ ID -2241

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What are the amplicon lengths of the KRAS and EGFR PCR kits?

FAQ ID -2289

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What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2201

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What does QIAGEN use for a positive control in precursor detection experiments?

FAQ ID -2212

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What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?

FAQ ID -2291

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What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?

FAQ ID -2297

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What are the corresponding QIAGEN names for former Biotage instruments?

FAQ ID -2285

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What buffer conditions give the best resolution for agarose gel electrophoresis?

FAQ ID -2256

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What can be the reason for low DNA yields after normalization with the SeqTarget System?

FAQ ID -2239

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How much buffer should be used for agarose gel electrophoresis?

FAQ ID -2257

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How much DNA should be loaded per well of an agarose gel?

FAQ ID -2259

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How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?

FAQ ID -2258

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How long does it take to generate results using the KRAS and EGFR PCR kits?

FAQ ID -2299

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How long should PCR fragments be for the SeqTarget System?

FAQ ID -2236

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How many miScript Precursor Assays do you offer?

FAQ ID -2213

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Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?

FAQ ID -2264

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Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?

FAQ ID -2268

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Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?

FAQ ID -2269

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If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?

FAQ ID -2265

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Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?

FAQ ID -2235

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Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?

FAQ ID -2243

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Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?

FAQ ID -1484

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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?

FAQ ID -1451

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What applications are offered on the QIAcube?

FAQ ID -1403

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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?

FAQ ID -1449

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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?

FAQ ID -1446

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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?

FAQ ID -1447

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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?

FAQ ID -1442

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What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?

FAQ ID -1468

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Can purification columns from other suppliers be processed on the QIAcube?

FAQ ID -1406

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Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?

FAQ ID -1414

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Are run reports and/or log files available on the QIAcube?

FAQ ID -1412

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Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?

FAQ ID -1480

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Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?

FAQ ID -1476

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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?

FAQ ID -1452

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Can different amounts of siRNA be ordered on one FlexiPlate?

FAQ ID -1473

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Can 2 µl reaction volumes be used with QuantiFast PCR Kits?

FAQ ID -1440

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Are there any specific cleaning solutions recommended for the QIAcube?

FAQ ID -1416

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Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?

FAQ ID -1439

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Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?

FAQ ID -1437

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Do I need to buy special kits for the QIAcube?

FAQ ID -1402

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Do I need to discard partially used QIAcube tip racks?

FAQ ID -1418

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What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?

FAQ ID -1463

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What are the turnaround times for FlexiPlate siRNA orders?

FAQ ID -1478

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What are the dimensions and weight of the QIAcube?

FAQ ID -1407

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How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?

FAQ ID -1438

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How many reactions can I perform with the new QuantiFast Kits for real-time PCR?

FAQ ID -1425

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If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?

FAQ ID -1477

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How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?

FAQ ID -1430

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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

FAQ ID -1415

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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?

FAQ ID -1450

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Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?

FAQ ID -1443

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What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?

FAQ ID -1436

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What is the detection limit of the QuantiFast Kits for real-time PCR?

FAQ ID -1432

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Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?

FAQ ID -1459

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Which cell lines have been tested with FastLane Cell RT-PCR Kits?

FAQ ID -1485

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Can I adjust the ROX concentration in the QuantiFast master mix?

FAQ ID -1427

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Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?

FAQ ID -1458

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Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?

FAQ ID -1482

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Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?

FAQ ID -1433

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Can FlexiPlate siRNA be ordered in solution on dry-ice?

FAQ ID -1474

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Can I program my own protocols for the QIAcube?

FAQ ID -1405

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How can I load new protocols onto the QIAcube?

FAQ ID -1421

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How can I get software updates for the QIAcube?

FAQ ID -1413

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How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?

FAQ ID -1441

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Do you offer trial-kit sizes for the new QuantiFast Kits?

FAQ ID -1429

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How can I decontaminate the QIAcube system?

FAQ ID -1419

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Can the QIAcube heater/shaker be used independently from protocol runs?

FAQ ID -1422

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Can the QIAcube rotor adapters be autoclaved?

FAQ ID -1409

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Can the QIAcube centrifuge be used independently from protocol runs?

FAQ ID -1423

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Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?

FAQ ID -1428

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Can the QIAcube be connected to a laboratory information management system?

FAQ ID -1411

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Can the QIAcube rotor and/or buckets be removed for cleaning?

FAQ ID -1410

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Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?

FAQ ID -1444

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Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?

FAQ ID -1431

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Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?

FAQ ID -1435

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What are the Tms of PCR products obtained using the miScript System?

FAQ ID -1599

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Do you have a protocol for cleanup of REPLI-g amplified DNA?

FAQ ID -1545

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What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1580

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What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?

FAQ ID -1596

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What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?

FAQ ID -1554

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Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?

FAQ ID -1553

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Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?

FAQ ID -1572

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Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?

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Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?

FAQ ID -1573

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Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?

FAQ ID -1587

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Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?

FAQ ID -1547

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Do you have a protocol for concentration of RNA in 96-well plates?

FAQ ID -1546

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Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

FAQ ID -1550

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Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?

FAQ ID -1592

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What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?

FAQ ID -1584

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What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1591

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Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?

FAQ ID -1579

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What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?

FAQ ID -1597

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Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?

FAQ ID -1575

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Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?

FAQ ID -1555

View

Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1583

View

Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?

FAQ ID -1576

View

Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?

FAQ ID -1577

View

Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?

FAQ ID -1585

View

Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?

FAQ ID -1571

View

Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1590

View

Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?

FAQ ID -1574

View

Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1586

View

How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?

FAQ ID -1598

View

How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

FAQ ID -1519

View

What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?

FAQ ID -2010

View

What is the advantage of using the Type-it Microsatellite PCR Kit?

FAQ ID -2059

View

What is the advantage of using the Type-it Mutation Detect PCR Kit?

FAQ ID -2064

View

What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?

FAQ ID -2005

View

What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?

FAQ ID -2029

View

What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?

-16

View

What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?

FAQ ID -2028

View

What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?

FAQ ID -2003

View

Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?

FAQ ID -2072

View

What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?

FAQ ID -2007

View

Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?

FAQ ID -2073

View

What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?

FAQ ID -2009

View

Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?

FAQ ID -2031

View

What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?

FAQ ID -2011

View

What are the features of the expression vector used for QIAgenes Insect/Mammalia?

FAQ ID -2050

View

Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?

FAQ ID -2035

View

Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?

FAQ ID -2068

View

Why is there more than one QIAgene for my gene of interest?

FAQ ID -2044

View

Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?

FAQ ID -2043

View

Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?

FAQ ID -2067

View

Will the optimized DNA sequence for QIAgenes be provided?

FAQ ID -2045

View

Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?

FAQ ID -2060

View

Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?

FAQ ID -2057

View

What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?

FAQ ID -2037

View

What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?

FAQ ID -2036

View

Can data be analyzed on the Rotor-Gene Q while a run is in progress?

FAQ ID -2091

View

Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?

FAQ ID -2048

View

Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?

FAQ ID -2066

View

Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?

FAQ ID -2058

View

Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?

FAQ ID -2030

View

Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?

FAQ ID -2079

View

Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?

FAQ ID -2092

View

Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?

FAQ ID -2093

View

What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?

FAQ ID -2004

View

What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?

FAQ ID -2034

View

Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?

FAQ ID -2049

View

Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?

FAQ ID -2038

View

What types of reaction vessels are required for use in the Rotor-Gene Q?

FAQ ID -2085

View

Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?

FAQ ID -2063

View

Can the EpiTect PCR Control DNAs be used for mouse and rat assays?

FAQ ID -2008

View

Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?

FAQ ID -2006

View

Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?

FAQ ID -2001

View

Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?

FAQ ID -2027

View

Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?

-15

View

Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?

FAQ ID -2074

View

Do all QIAsymphony DNA Investigator protocols use carrier RNA?

FAQ ID -2033

View

Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?

FAQ ID -2052

View

Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?

FAQ ID -2065

View

Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?

FAQ ID -2062

View

Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?

FAQ ID -2082

View

How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?

FAQ ID -2083

View

Can membrane proteins be expressed with QIAgenes expression kits?

FAQ ID -2047

View

Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?

FAQ ID -2000

View

Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?

FAQ ID -2040

View

Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?

FAQ ID -2039

View

Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?

FAQ ID -2081

View

How can a C-terminal 6xHis-tagged QIAgene be expressed?

FAQ ID -2041

View

How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?

FAQ ID -2051

View

Does the Rotor-Gene Q software have the capacity to export data?

FAQ ID -2089

View

Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?

FAQ ID -2032

View

Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?

FAQ ID -2070

View

How fast does the rotor spin during a run on the Rotor-Gene Q?

FAQ ID -2080

View

How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?

FAQ ID -2088

View

How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?

FAQ ID -2002

View

How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?

FAQ ID -2095

View

How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?

FAQ ID -2071

View

If orders cannot be placed online, how can QIAgenes be ordered?

FAQ ID -2046

View

If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?

FAQ ID -2087

View

How long does a single run on the PyroMark Q24 take?

FAQ ID -2096

View

How much space does the PyroMark Q24 instrument need?

FAQ ID -2098

View

Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?

FAQ ID -2061

View

How accurate and reliable is PyroMark Q24 in mutation analysis?

FAQ ID -2094

View

Is there a charge for user training on the QIAsymphony SP System?

FAQ ID -1900

View

Is the QIAsymphony RNA Kit compatible with stabilized samples?

FAQ ID -1944

View

Is sample pretreatment required for purifying RNA on the QIAsymphony SP?

FAQ ID -1938

View

Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?

FAQ ID -1920

View

What are the expected DNA yields from tissue processed on the QIAsymphony SP?

FAQ ID -1919

View

QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?

FAQ ID -1982

View

What are the dimensions and weight of the QIAsymphony SP System?

FAQ ID -1901

View

What is the range of elution volumes on the QIAsymphony SP?

FAQ ID -1908

View

How many PCR reactions can be performed for different scales of miScript Primer Assays?

FAQ ID -1990

View

How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?

FAQ ID -1940

View

How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?

FAQ ID -1905

View

How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?

FAQ ID -1994

View

How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?

FAQ ID -1941

View

How much sample can be used with the Allprep DNA/RNA 96 Kit?

FAQ ID -1997

View

How much sample can be used with the RNeasy Plus 96 Kit?

FAQ ID -1992

View

How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?

FAQ ID -1917

View

What sample types can be used with the AllPrep DNA/RNA 96 Kit?

FAQ ID -1996

View

Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?

FAQ ID -1937

View

What sample types can be used with the RNeasy Plus 96 Kit?

FAQ ID -1991

View

Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?

FAQ ID -1921

View

What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?

FAQ ID -1918

View

Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?

FAQ ID -1999

View

Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?

FAQ ID -1993

View

What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?

FAQ ID -1985

View

Is it possible to modify QIAsymphony protocols?

FAQ ID -1903

View

What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?

FAQ ID -1939

View

What are the expected yields of total RNA isolated with the QIAsymphony SP?

FAQ ID -1942

View

What kind of molecules are miScript miRNA Inhibitors?

FAQ ID -1983

View

What sample input volumes can be used on the QIAsymphony SP?

FAQ ID -1907

View

What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?

FAQ ID -1915

View

What kind of molecules are miScript miRNA Mimics?

FAQ ID -1986

View

What RNA elution volumes are available with the QIAsymphony RNA Kit?

FAQ ID -1943

View

Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

FAQ ID -1916

View

Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?

FAQ ID -1976

View

Can uneven sample numbers be processed on the QIAsymphony SP module?

FAQ ID -1906

View

Does the QIAsymphony SP system generate run reports and/or log files?

FAQ ID -1911

View

Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?

FAQ ID -1984

View

Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?

FAQ ID -1964

View

Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?

FAQ ID -1953

View

Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?

FAQ ID -1960

View

Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?

FAQ ID -1969

View

Can I program my own protocols for the QIAsymphony SP?

FAQ ID -1904

View

Are QuantiFast Multiplex PCR Kits available in trial sizes?

FAQ ID -1981

View

Are there any specific cleaning solutions recommended for the QIAsymphony SP?

FAQ ID -1913

View

Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?

FAQ ID -1978

View

Can the QIAsymphony RNA Kit be used to purify RNA from blood?

FAQ ID -1945

View

Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?

FAQ ID -1909

View

Can protocol runs on the QIAsymphony SP be interrupted and resumed?

FAQ ID -1912

View

Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?

FAQ ID -1979

View

Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?

FAQ ID -1995

View

Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?

FAQ ID -1950

View

Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?

FAQ ID -1951

View

Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?

FAQ ID -1959

View

Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?

FAQ ID -1975

View

Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?

FAQ ID -1946

View

Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?

FAQ ID -1947

View

Do you have a protocol for the purification of DNA from fruit flies?

FAQ ID -1970

View

Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?

FAQ ID -1974

View

How can the QIAsymphony SP system be decontaminated?

FAQ ID -1914

View

Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?

FAQ ID -1958

View

Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?

FAQ ID -1955

View

Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?

FAQ ID -1954

View

Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?

FAQ ID -1967

View

Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?

FAQ ID -1968

View

Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?

FAQ ID -1948

View

Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?

FAQ ID -1972

View

Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?

FAQ ID -1973

View

Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?

FAQ ID -1971

View

Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?

FAQ ID -1965

View

Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?

FAQ ID -1952

View

What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?

FAQ ID -1751

View

What is the fidelity of TopTaq DNA Polymerase?

FAQ ID -1739

View

Is TopTaq DNA Polymerase a hot-start polymerase?

FAQ ID -1748

View

Which nucleotide analogs can be used with TopTaq DNA Polymerase?

FAQ ID -1741

View

Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?

FAQ ID -1755

View

What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?

FAQ ID -1738

View

What is the shelf-life of TopTaq DNA Polymerase and Master Mix?

FAQ ID -1743

View

What is the principle of the REPLI-g FFPE Kit?

FAQ ID -1753

View

What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?

FAQ ID -1752

View

What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?

FAQ ID -1750

View

Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?

FAQ ID -1737

View

What is the recommended sample size for use with the REPLI-g FFPE Kit?

FAQ ID -1756

View

Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?

FAQ ID -1749

View

Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?

FAQ ID -1702

View

Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?

FAQ ID -1740

View

At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?

FAQ ID -1758

View

Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?

FAQ ID -1714

View

How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?

FAQ ID -1734

View

Do you have protocols for sample processing on the Autopure LS instrument?

FAQ ID -1759

View

Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?

FAQ ID -1754

View

Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?

FAQ ID -1760

View

Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?

FAQ ID -1733

View

Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?

FAQ ID -1744

View

Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?

FAQ ID -1761

View

Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?

FAQ ID -1745

View

Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?

FAQ ID -1666

View

What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?

FAQ ID -1601

View

What is the binding capacity of the Ni-NTA Superflow Cartridges?

FAQ ID -1603

View

Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1610

View

Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?

FAQ ID -1697

View

Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?

FAQ ID -1609

View

Is sequence information for HP Validated siRNAs provided in the FlexiTube format?

FAQ ID -1665

View

Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1614

View

Which siRNA controls are available in FlexiTube format?

FAQ ID -1661

View

Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?

FAQ ID -1690

View

Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?

FAQ ID -1620

View

What is the composition of Buffer PE?

FAQ ID -1691

View

What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1616

View

Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?

FAQ ID -1606

View

Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?

FAQ ID -1607

View

Can I store cDNA generated by the miScript System?

FAQ ID -1602

View

Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?

FAQ ID -1612

View

Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?

FAQ ID -1615

View

Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?

FAQ ID -1617

View

Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?

FAQ ID -1618

View

Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?

FAQ ID -1664

View

Can I still reorder siRNA that has been removed from the GeneGlobe data base?

FAQ ID -1668

View

Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?

FAQ ID -1608

View

Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?

FAQ ID -1667

View

Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?

FAQ ID -1658

View

Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?

FAQ ID -1611

View

How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?

FAQ ID -1644

View

How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?

FAQ ID -1605

View

How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1613

View

How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?

FAQ ID -1623

View

Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?

FAQ ID -1619

View

Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?

FAQ ID -1659

View

Do you have a protocol for purification of total RNA from plant latex?

FAQ ID -1694

View

Do I need to use the miScript Universal Primer for detection of mRNA?

FAQ ID -1600

View

Do you offer a QuantiFast Kit for one-step RT-PCR?

FAQ ID -1695

View

Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?

FAQ ID -1693

View

How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?

FAQ ID -2159

View

How often can the Strep-Tactin Superflow Plus resin be reused?

FAQ ID -2169

View

If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2129

View

If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?

FAQ ID -2150

View

Is it normal that the binding capacity of GST-resins decreases with flow rate?

FAQ ID -2174

View

Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?

FAQ ID -2172

View

Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?

FAQ ID -2196

View

Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?

FAQ ID -2118

View

Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?

FAQ ID -2122

View

Why do I need normalization using Rotor-Gene ScreenClust HRM Software?

FAQ ID -2198

View

Why is there an asterisk for my miScript Primer Assays?

FAQ ID -2176

View

Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?

FAQ ID -2121

View

Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?

FAQ ID -2139

View

Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?

FAQ ID -2124

View

What is miRNA*? Do I need to consider this in my profiling experiment?

FAQ ID -2179

View

What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?

FAQ ID -2119

View

What are possible reasons for reduced DNA yields with REPLI-g Kits?

FAQ ID -2148

View

What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?

FAQ ID -2199

View

What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2158

View

What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?

FAQ ID -2109

View

What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?

FAQ ID -2144

View

What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?

FAQ ID -2145

View

How should DNA isolated on the QIAsymphony be stored?

FAQ ID -2165

View

How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2157

View

How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?

FAQ ID -2193

View

Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?

FAQ ID -2188

View

Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?

FAQ ID -2173

View

Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?

FAQ ID -2160

View

What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?

FAQ ID -2156

View

Which plate formats are offered for QuantiTect Primer Assay Plates?

FAQ ID -2106

View

What species are QuantiTect Primer Assay Plates offered for?

FAQ ID -2110

View

Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?

FAQ ID -2147

View

Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?

FAQ ID -2154

View

How many reactions can be performed per well of the QuantiTect Primer Assay Plates?

FAQ ID -2107

View

Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?

FAQ ID -2190

View

Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?

FAQ ID -2108

View

Can Glutathione Superflow resin be reused?

FAQ ID -2171

View

Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?

FAQ ID -2138

View

Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?

FAQ ID -2112

View

Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?

FAQ ID -2168

View

Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?

FAQ ID -2134

View

Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2130

View

Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2131

View

Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?

FAQ ID -2142

View

Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?

FAQ ID -2135

View

Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?

FAQ ID -2191

View

Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

FAQ ID -2116

View

Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?

FAQ ID -2136

View

Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?

FAQ ID -2111

View

Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?

FAQ ID -2126

View

How does template quality influence the results with the Type-it HRM PCR Kit?

FAQ ID -2197

View

How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?

FAQ ID -2166

View

Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?

FAQ ID -2105

View

How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?

FAQ ID -2189

View

How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?

FAQ ID -2125

View

How many elution steps are recommended when using Glutathione Superflow resin?

FAQ ID -2175

View

How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?

FAQ ID -2192

View

Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?

FAQ ID -2104

View

Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2163

View

Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?

FAQ ID -2162

View

Can Vapor-Lock be used with samples processed on the QIAgility?

FAQ ID -2153

View

Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?

FAQ ID -2123

View

Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?

FAQ ID -2117

View

Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?

FAQ ID -2128

View

What main input voltage is required for the QIAxcel Analyzer?

FAQ ID -1821

View

What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?

FAQ ID -1852

View

Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?

FAQ ID -1882

View

The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?

FAQ ID -1850

View

Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?

FAQ ID -1832

View

What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?

FAQ ID -1834

View

Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??

FAQ ID -1847

View

Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?

FAQ ID -1844

View

Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?

FAQ ID -1851

View

Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?

FAQ ID -1828

View

Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?

FAQ ID -1841

View

Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?

FAQ ID -1861

View

How can a positive threshold be set when running the QIAxcel System?

FAQ ID -1843

View

How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System?

FAQ ID -1827

View

How long will the intensity calibration procedure on the QIAxcel System take?

FAQ ID -1826

View

Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?

FAQ ID -1824

View

Will the QIAxcel System run using a green Data Review Software Key?

FAQ ID -1853

View

Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?

FAQ ID -1848

View

Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?

FAQ ID -1835

View

When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?

FAQ ID -1842

View

What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?

FAQ ID -1833

View

Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?

FAQ ID -1837

View

When should an intensity calibration be re-performed on the QIAxcel System?

FAQ ID -1825

View

When should the N2 bottle used with the QIAxcel System be changed?

FAQ ID -1830

View

Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?

FAQ ID -1855

View

Can the QIAxcel System or QIAxcel Advanced be connected to a network?

FAQ ID -1862

View

How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?

FAQ ID -1836

View

Can multiple QIAxcel Systems be run from one computer?

FAQ ID -1860

View

If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?

FAQ ID -1858

View

If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?

FAQ ID -1857

View

How often should the alignment marker for the QIAxcel System be changed?

FAQ ID -1839

View

How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?

FAQ ID -1840

View

In the results table generated with the QIAxcel System, what does "normalized area" mean?

FAQ ID -1854

View

How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?

FAQ ID -1823

View

If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?

FAQ ID -1856

View

How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?

FAQ ID -1838

View

What is the composition of buffer QBT?

FAQ ID -411

View

What is the composition of buffer QC?

FAQ ID -412

View

What is the composition of buffer P3?

FAQ ID -418

View

What is the composition of buffer QF?

FAQ ID -413

View

What is the composition of buffer TE?

FAQ ID -416

View

What is the composition of buffer QN?

FAQ ID -414

View

What is the composition of buffer OCD?

FAQ ID -423

View

What is the composition of buffer FWB2?

FAQ ID -417

View

What is the composition of buffer OBB?

FAQ ID -421

View

What is the composition of buffer OW2?

FAQ ID -419

View

What is the composition of buffer OL2?

FAQ ID -424

View

What is the composition of buffer OCL?

FAQ ID -422

View

Can RNAi experiments be performed in Drosophila?

FAQ ID -436

View

What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?

FAQ ID -470

View

Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?

FAQ ID -472

View

Has RNAi been successful using siRNA in Zebrafish and Xenopus?

FAQ ID -400

View

How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?

FAQ ID -468

View

How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?

FAQ ID -498

View

Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?

FAQ ID -403

View

Can QIAquick Kits be used to clean up RNA samples?

FAQ ID -490

View

Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?

FAQ ID -482

View

Can Ni-NTA resins be used to purify protein with an internal His-tag?

FAQ ID -496

View

What is the composition of buffer STE?

FAQ ID -415

View

Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?

FAQ ID -429

View

What is the composition of buffer OEB?

FAQ ID -420

View

Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?

FAQ ID -474

View

Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?

FAQ ID -467

View

Why is the pQE DNA provided in QIAexpress Kits blue in color?

FAQ ID -487

View

Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?

FAQ ID -473

View

What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?

FAQ ID -432

View

What is the minimum elution volume when using the RNeasy Micro Kit?

FAQ ID -428

View

What is the smallest sample size that can be used with RNeasy Mini Kits?

FAQ ID -485

View

What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?

FAQ ID -426

View

What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?

FAQ ID -480

View

What is a homogeneous LiquiChip assay?

FAQ ID -476

View

What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?

FAQ ID -479

View

What is the binding capacity of the RNeasy Micro Kit column?

FAQ ID -427

View

What is the composition and size of the LiquiChip Beads?

FAQ ID -475

View

What is the binding capacity of the RNeasy MinElute Cleanup Kit column?

FAQ ID -430

View

Is dihydrofolate reductase (DHFR) immunogenic?

FAQ ID -471

View

What are some of the assays that are adaptable to the LiquiChip System?

FAQ ID -477

View

My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?

-20

View

Will REPLI-g work at high temperatures?

FAQ ID -656

View

What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?

FAQ ID -622

View

The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?

FAQ ID -652

View

What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?

FAQ ID -663

View

What is the shelf-life of my QIAGEN Kit?

FAQ ID -651

View

What is the recommended incubation time to stabilize tissue RNA in RNAprotect?

FAQ ID -647

View

What do you mean by “Quantification of locus representation” in REPLI-g WGA product?

FAQ ID -698

View

What is the average amount of DNA and RNA present in 1 ml normal serum?

FAQ ID -635

View

What is REPLI-g whole genome amplification?

FAQ ID -654

View

What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?

FAQ ID -631

View

What do you recommend for the cleanup of genomic DNA (gDNA)?

FAQ ID -618

View

What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?

FAQ ID -632

View

What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?

FAQ ID -601

View

What is the length of the MDA whole genome amplification product?

FAQ ID -690

View

What 96-well plates do you recommend for use with the LiquiChip Workstation?

FAQ ID -630

View

Is it possible to perform laser microdissection with RNAprotect-stabilized tissue?

FAQ ID -608

View

Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?

FAQ ID -602

View

What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?

FAQ ID -646

View

What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?

FAQ ID -665

View

What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?

FAQ ID -686

View

Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?

FAQ ID -675

View

Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?

FAQ ID -633

View

Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?

FAQ ID -603

View

Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?

FAQ ID -604

View

Why do you recommend using Triton X for the purification of 6xHis-tagged protein?

-100

View

Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?

FAQ ID -644

View

Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?

FAQ ID -637

View

Will TA cloning work with REPLI-g WGA product?

FAQ ID -672

View

Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?

FAQ ID -664

View

What is the stability of the REPLI-g MDA product?

FAQ ID -693

View

When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?

FAQ ID -653

View

Where should I add a dye-label modification on the siRNA?

FAQ ID -648

View

What types of materials from environmental samples could interfere with WGA?

FAQ ID -666

View

What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?

FAQ ID -600

View

Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?

FAQ ID -615

View

Can I run less than 15 samples on the BioSprint 15 instrument?

FAQ ID -621

View

Can I re-amplify whole genome amplification product?

FAQ ID -670

View

Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?

FAQ ID -645

View

Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?

FAQ ID -616

View

Do you have an off-the shelf diagnostics kit for WGA?

FAQ ID -678

View

Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?

FAQ ID -619

View

Can the computer for the LiquiChip Workstation be networked?

FAQ ID -629

View

Are there components in biological samples that can inhibit the REPLI-g reaction?

FAQ ID -667

View

Are the BioSprint 15/96 instruments stand-alone workstations?

FAQ ID -620

View

Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?

FAQ ID -683

View

Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?

FAQ ID -638

View

Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?

FAQ ID -643

View

Can I do whole genome amplification from brain tissue using REPLI-g?

FAQ ID -669

View

Can FTA paper for dried blood spots be used with REPLI-g?

FAQ ID -685

View

Can I do whole genome amplification from mitochondrial DNA using REPLI-g?

FAQ ID -668

View

Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?

FAQ ID -671

View

How can I minimize degradation of RNA from my pancreas sample?

FAQ ID -624

View

How can I quantify the amount of REPLI-g DNA I have amplified?

FAQ ID -694

View

How should I store the REPLI-g amplification product?

FAQ ID -692

View

Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?

FAQ ID -682

View

How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?

FAQ ID -614

View

Do I need to clean up the REPLI-g amplification product for downstream analysis?

FAQ ID -691

View

Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?

FAQ ID -673

View

Do you have a kit for RNA isolation from any kind of sample type?

FAQ ID -627

View

How can I improve my RNA yield from liver sample when using RNeasy Kits?

FAQ ID -623

View

How can I determine the quality of my REPLI-g amplified products?

FAQ ID -695

View

Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?

FAQ ID -684

View

What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?

FAQ ID -606

View

Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?

FAQ ID -599

View

I want to start with gene silencing (RNAi) experiments. Do you have informational literature?

FAQ ID -580

View

What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?

FAQ ID -572

View

What is a quenched FRET assay?

FAQ ID -542

View

Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?

FAQ ID -573

View

What do you recommend for isolating RNA from more than 100 mg of plant tissue?

FAQ ID -570

View

What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?

FAQ ID -514

View

What has to be done to a RNA sample before loading it onto an Agilent Bioanalyzer?

FAQ ID -528

View

How should I dissolve the lyophilized Anti-His and Tag Antibodies?

-100

View

Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?

FAQ ID -576

View

Is it possible to perform a UNG treatment when using QuantiTect kits?

FAQ ID -564

View

Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?

FAQ ID -563

View

Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?

FAQ ID -519

View

Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?

FAQ ID -532

View

Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?

FAQ ID -533

View

Why are my realtime PCR amplification plots hook-shaped?

FAQ ID -587

View

How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?

FAQ ID -579

View

If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?

FAQ ID -513

View

How should I store cDNA produced using Omniscript Reverse Transcriptase?

FAQ ID -561

View

Which chloroform should I use for the RNeasy Lipid Tissue Kit?

FAQ ID -516

View

Will pancreas tissue swim on top of RNAprotect?

FAQ ID -536

View

Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?

FAQ ID - 525

View

Why does my realtime PCR assay quality decrease over time?

FAQ ID -589

View

Why do replicates in real-time PCR have different plateau heights?

FAQ ID -539

View

What is the longest fragment that was cloned with the pQE UA Cloning Vector?

-30

View

What is the difference between BSA-free and BSA-containing RGS-His Antibody?

FAQ ID -569

View

What is the composition of Buffer ER? Is it available separately?

FAQ ID -571

View

What is the composition of the siRNA resuspension & annealing buffer?

FAQ ID -522

View

When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?

FAQ ID -534

View

Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?

FAQ ID -562

View

What reaction volume should I use with QuantiTect Kits?

FAQ ID -551

View

What is Tissue-Tek O.C.T., and what is it used for?

FAQ ID -530

View

What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?

FAQ ID -566

View

How many template copies can I detect using QuantiTect Kits in real-time PCR?

FAQ ID -543

View

Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?

FAQ ID -515

View

Does DEPC harm RNA?

FAQ ID -529

View

Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?

FAQ ID -527

View

Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?

FAQ ID -523

View

Can the MAPK1 control siRNA be used for rat cells?

FAQ ID -547

View

Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?

FAQ ID -581

View

Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?

FAQ ID -577

View

After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?

FAQ ID -512

View

Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?

FAQ ID -584

View

Can I shorten the activation time for the HotStarTaq DNA Polymerase?

FAQ ID -565

View

Can I homogenize samples in QIAzol using the QIAshredder?

FAQ ID -501

View

Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?

FAQ ID -560

View

How can I avoid primer-dimer formation during PCR amplification?

FAQ ID -544

View

Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?

FAQ ID -594

View

Do you provide control proteins for use with the PhosphoProtein Purification Kit?

FAQ ID -596

View

How long should the amplicon be if I am using QuantiTect SYBR Green detection?

FAQ ID -553

View

How is an antibody epitope determined?

FAQ ID -593

View

Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?

FAQ ID -540

View

How can I tell if I have primer-dimers in my PCR reaction?

FAQ ID -552

View

Do we recommend pooling siRNA sequences?

FAQ ID -517

View

Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?

FAQ ID -575

View

Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?

FAQ ID -598

View

Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?

FAQ ID -548

View

Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?

FAQ ID -567

View

Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?

FAQ ID -518

View

Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?

FAQ ID -500

View

Do you have a protocol for isolating RNA from yeast using RNeasy?

FAQ ID -535

View

Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?

FAQ ID -597

View

Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?

FAQ ID -931

View

Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?

FAQ ID -926

View

Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?

FAQ ID -979

View

Do you have a protocol for the isolation of DNA from tofu?

FAQ ID -932

View

Do you have a protocol for Streptokinase treatment of clotted blood?

FAQ ID -912

View

Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?

FAQ ID -973

View

Do you have a protocol for the isolation of RNA from leukocytes in milk?

FAQ ID -952

View

Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?

FAQ ID -975

View

Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?

FAQ ID -974

View

Do you have a protocol for the isolation of total RNA from buccal swabs?

FAQ ID -956

View

Do you have a protocol for the isolation of viral DNA from stool?

FAQ ID -929

View

Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?

FAQ ID -949

View

Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?

FAQ ID -976

View

Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?

FAQ ID -951

View

Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?

FAQ ID -924

View

Do you have a protocol for the isolation of genomic DNA from sperm?

FAQ ID -909

View

Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?

FAQ ID -922

View

Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?

FAQ ID -992

View

Do you have a protocol for the isolation of genomic DNA from fungi?

FAQ ID -911

View

Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?

FAQ ID -955

View

Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

FAQ ID -900. Test

View

Do you have a protocol for the preparation of RNAprotect preserved tissues for pathological studies?

FAQ ID -950

View

Do you have a protocol for the purification of DNA from cells using the BioSprint System?

FAQ ID -977

View

Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?

FAQ ID -982

View

Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?

FAQ ID -902

View

Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?

FAQ ID -970

View

Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?

FAQ ID -983

View

Do you have a protocol for GMO testing of food samples?

FAQ ID -921

View

Do you have a protocol for isolation of bacterial DNA from soil?

FAQ ID -923

View

Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?

FAQ ID -901

View

Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?

FAQ ID -917

View

Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?

FAQ ID -919

View

Do you have a protocol for isolation of genomic DNA from insects?

FAQ ID -904

View

Do you have a protocol for detection of HBV DNA by PCR?

FAQ ID -915

View

Can the BioSprint instruments be placed in a cool room?

FAQ ID -938

View

Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -937

View

Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?

FAQ ID -913

View

Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?

FAQ ID -969

View

Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?

FAQ ID -959

View

Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?

FAQ ID -965

View

Do you have a protocol for cleanup of non-radioactive DNA samples?

FAQ ID -945

View

Do you have a protocol for radioactive labelling of cDNA?

FAQ ID -960

View

Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?

FAQ ID -971

View

Do you have a protocol for the isolation of genomic DNA from bone?

FAQ ID -908

View

Do you have a protocol for the isolation of viral RNA from stool?

FAQ ID -918

View

Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?

FAQ ID -907

View

Do you have protocols for multiple extractions of DNA fragments from agarose gels?

FAQ ID -944

View

Why is it not recommended to stabilize cells with RNAprotect?

FAQ ID -941

View

What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?

FAQ ID -942

View

Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?

FAQ ID -997

View

Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?

FAQ ID -914

View

Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?

FAQ ID -985

View

Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?

FAQ ID -990

View

Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?

FAQ ID -966

View

Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?

FAQ ID -972

View

Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?

FAQ ID -961

View

Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?

FAQ ID -967

View

Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?

FAQ ID -934

View

Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?

FAQ ID -947

View

Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?

FAQ ID -935

View

Do you have a protocol for the purification of PCR products using the BioSprint System?

FAQ ID -984

View

Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?

FAQ ID -905

View

Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?

FAQ ID -903

View

Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?

FAQ ID -397

View

Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?

FAQ ID -358

View

Is QIAGEN carrier RNA sold separately?

FAQ ID -351

View

I accidentally added RNAprotect to my cells, and now the cells are difficult to pellet. What can I do?

FAQ ID -364

View

What are the calculated molecular weights of the TAGZyme enzymes?

FAQ ID -329

View

Is it possible to store TAGzyme enzymes at°C?

-80

View

Is Q-Solution required for PCR with QIAGEN's PCR kits?

FAQ ID -380

View

How do I know if my plasmid is a high- or low copy number type?

FAQ ID -350

View

How do I perform a DNA precipitation to concentrate my sample?

FAQ ID -305

View

How can QIAGEN Protease and Proteinase K be inactivated?

FAQ ID -315

View

How complete is the removal of DAPase in the TAGZyme process?

FAQ ID -319

View

How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?

FAQ ID -392

View

How many transfections can I perform with 20 nmol of HPP grade siRNA?

FAQ ID -367

View

How do I submit a siRNA order by telephone or online?

FAQ ID -399

View

What is the white insoluble precipitate in my resuspended plasmid DNA pellet?

FAQ ID -352

View

What kits does QIAGEN offer to extract RNA from whole blood?

FAQ ID -304

View

Why do I get genomic DNA contamination in my plasmid prep?

FAQ ID -353

View

Which primers can I use for sequencing pQE-expression vector constructs?

FAQ ID -343

View

What is the standard assay volume recommended for LiquiChip assays?

FAQ ID -330

View

What is the average molecular weight of a siRNA, and how do I convert uM to ug values?

FAQ ID -388

View

What are the dissociation constants for the Anti-His Antibodies?

FAQ ID -385

View

What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?

FAQ ID -307

View

What is the origin of replication and the plasmid copy number of the pQE vectors?

FAQ ID -338

View

What is the acceptable maximum g force for centrifugation of Xa Removal Resin?

FAQ ID -320

View

What is the composition of Buffer FG3?

FAQ ID -356

View

What is the composition of PBS?

FAQ ID -361

View

How can I keep my centrifuge tubes endotoxin-free?

FAQ ID -301

View

Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?

FAQ ID -354

View

303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?

View

Can I store agarose gel slices containing DNA for gel extraction at a later point?

FAQ ID -313

View

Can I use a partial MinElute 96 UF plate?

FAQ ID -355

View

Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?

FAQ ID -372

View

Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?

FAQ ID -306

View

Are QIAprep and QIAquick Spin columns interchangeable?

FAQ ID -311

View

Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?

FAQ ID -308

View

Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?

FAQ ID -359

View

Are QIAGEN monoclonal Anti His Antibodies glycosylated?

FAQ ID -334

View

Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?

FAQ ID -366

View

Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?

FAQ ID -332

View

Can Buffers N3 and P3 be used interchangeably?

FAQ ID -310

View

Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?

FAQ ID -328

View

How are siRNAs introduced into C. elegans?

FAQ ID -396

View

Do I need to anneal, deprotect or desalt my QIAGEN siRNA?

FAQ ID -398

View

Do pQE vectors contain the CAT gene?

FAQ ID -362

View

How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

FAQ ID -374

View

How can I improve the expression of proteins containing hydrophobic regions?

FAQ ID -339

View

How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?

FAQ ID -324

View

How can I express toxic protein in E. coli?

FAQ ID -373

View

Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?

FAQ ID -323

View

Can RNAprotect Tissue Reagent be used to store previously isolated RNA?

FAQ ID -337

View

Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?

FAQ ID -360

View

Can siRNA silence bacterial genes?

FAQ ID -395

View

Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?

FAQ ID -312

View

Does the 5x OneStep RT-PCR Buffer contain BSA?

FAQ ID -326

View

Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?

FAQ ID -327

View

Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?

FAQ ID -318

View

What are exo-resistant random hexamers used in the REPLI-g reaction?

FAQ ID -710

View

The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?

FAQ ID -776

View

Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?

FAQ ID -797

View

What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?

FAQ ID -721

View

What are the expected average DNA yields from plant extractions on the BioSprint 96?

FAQ ID -771

View

What is the basic technology behind the Strep-tag Protein Purification System?

FAQ ID -740

View

What is the advantage of running an analytical gel with fractions of my plasmid preparation?

FAQ ID -769

View

What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

FAQ ID -728

View

I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?

FAQ ID -756

View

If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?

FAQ ID -718

View

How to quantitate DNA purified using the BioSprint 96?

FAQ ID -773

View

How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?

FAQ ID -734

View

I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?

-20

View

Is it possible to use LiquiChip beads for FACS applications?

FAQ ID -733

View

Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?

FAQ ID -785

View

I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?

FAQ ID -745

View

Is GFP excited and detected by the green laser of the LiquiChip System?

FAQ ID -737

View

Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?

FAQ ID -763

View

Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?

FAQ ID -755

View

Why are some of my RNAprotect-stabilized samples frozen atºC while others are not?

ID -758

View

Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?

FAQ ID -719

View

Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?

FAQ ID -715

View

Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?

FAQ ID -703

View

Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?

FAQ ID -713

View

Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?

FAQ ID-751

View

Why is my plasmid DNA yield low?

FAQ ID -768

View

What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?

FAQ ID -764

View

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

FAQ ID -761

View

Why is there no detection of free reporter fluorescence in the LiquiChip System?

FAQ ID -732

View

What is the composition of buffer AE?

FAQ ID -730

View

What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?

FAQ ID -782

View

What is the composition of Buffer N3?

FAQ ID -767

View

What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?

FAQ ID -779

View

Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?

FAQ ID -766

View

What type of Sequencing Services does QIAGEN offer?

FAQ ID -781

View

What is the enzyme used in the REPLI-g reaction?

FAQ ID -704

View

What is the recipe for SOC medium?

FAQ ID -798

View

What is the general sensitivity level of the LiquiChip System?

FAQ ID -731

View

How should fluorescent labeled probes be stored?

FAQ ID -784

View

Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?

FAQ ID -741

View

Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?

FAQ ID -757

View

Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?

FAQ ID -778

View

Can lyophilized plant material be used for DNA extraction on the BioSprint 96?

FAQ ID -772

View

Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?

FAQ ID -717

View

Can the REPLI-g WGA enzyme work with labeled dNTPs?

FAQ ID -706

View

Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?

FAQ ID -744

View

Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?

FAQ ID -795

View

Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?

FAQ ID -722

View

Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?

FAQ ID -791

View

Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?

FAQ ID -770

View

Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?

FAQ ID -786

View

Any data on the fidelity of the REPLI-g MDA technique?

FAQ ID -707

View

Are Centromeres and Telomeres amplified using REPLI-g WGA?

FAQ ID -702

View

Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?

FAQ ID -712

View

Can I use REPLI-g for SNP Genotyping?

FAQ ID -700

View

Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?

FAQ ID -716

View

Can I purchase Phi29 DNA polymerase only?

FAQ ID -708

View

How can I get specific information and a quote for my individual Sequencing Service needs?

FAQ ID -792

View

How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?

FAQ ID -780

View

How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?

FAQ ID -788

View

How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?

FAQ ID -762

View

How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?

FAQ ID -783

View

How many cells can be used with the FastLane Cell cDNA Kit?

FAQ ID -796

View

Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?

FAQ ID -746

View

Do I have to switch off the laser of the LiquiChip Workstation overnight?

FAQ ID -736

View

Can frozen blood be used for whole genome amplification with REPLI-g?

FAQ ID -794

View

How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?

FAQ ID -738

View

How much DNA is obtained in the average PCR reaction?

FAQ ID -750

View

Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?

FAQ ID -714

View

Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?

FAQ ID -754

View

Do you synthesize thiophosphate modified random hexamers?

FAQ ID -711

View

Has anyone verified whole genome amplification accuracy with Sequencing?

FAQ ID -701

View

How best to handle the light sensitive LiquiChip reagents?

FAQ ID -735

View

Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?

FAQ ID -793

View

Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?

FAQ ID -759

View

Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?

FAQ ID -729

View

Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?

FAQ ID -1019

View

Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?

FAQ ID -1040

View

How can I prevent running out of M48 Kit reagents before the full number of samples are processed?

FAQ ID -1042

View

What are the additional plasmid bands I see on my gel?

FAQ ID -1059

View

Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?

FAQ ID -1063

View

Is it possible to use the PhosphoProtein Purification Kit in batch format?

FAQ ID -1069

View

What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?

FAQ ID -1077

View

What is the difference between Buffers RLT and RLT Plus?

FAQ ID -1043

View

What is the difference between TurboCapture Microplate Holders Type A and Type B?

FAQ ID -1038

View

Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?

FAQ ID -1071

View

What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?

FAQ ID -1093

View

Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?

FAQ ID -1049

View

How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?

FAQ ID -1046

View

How can I tell TurboCapture Microplate Holder Type A from Type B?

FAQ ID -1039

View

How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?

FAQ ID -1086

View

I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?

FAQ ID -1045

View

How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?

FAQ ID -1058

View

What is the error rate of HotStar HiFidelity DNA Polymerase?

FAQ ID -1048

View

How do I perform an Acetone Precipitation for concentrating and desalting protein samples?

FAQ ID -1035

View

Which kit should I use for RNA isolation from Cartilage?

FAQ ID -1026

View

Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?

FAQ ID -1092

View

Where can I find the serial number on QIAGEN's BioRobots?

FAQ ID -1064

View

Which kit can be used for simultaneous purification of DNA and RNA from biopsies?

FAQ ID -1089

View

Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?

FAQ ID -1083

View

Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?

FAQ ID -1044

View

Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?

FAQ ID -1054

View

Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?

FAQ ID -1053

View

Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?

FAQ ID -1084

View

Where can I find a protocol for cleanup of already purified plasmid DNA?

FAQ ID -1031

View

What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?

FAQ ID -1090

View

What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?

FAQ ID -1047

View

What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?

FAQ ID -1079

View

What is the recipe for 1x PBS solution?

FAQ ID -1030

View

What should I use as a standard for absolute quantification in real-time PCR?

FAQ ID -1085

View

What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?

FAQ ID -1055

View

What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?

FAQ ID -1034

View

What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?

FAQ ID -1067

View

How can I prevent clogging of QIAfilter cartridges?

FAQ ID -1060

View

Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?

FAQ ID -1037

View

Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?

FAQ ID -1065

View

Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?

FAQ ID -1050

View

Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?

FAQ ID -1052

View

Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?

FAQ ID -1020

View

Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?

FAQ ID -1017

View

Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?

FAQ ID -1032

View

Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?

FAQ ID -1007

View

Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?

FAQ ID -1091

View

Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?

FAQ ID -1051

View

Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?

FAQ ID -1025

View

Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -1078

View

Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?

FAQ ID -1036

View

Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?

FAQ ID -1066

View

Can the AllPrep RNA/Protein Kit be used with tissue?

FAQ ID -1095

View

Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?

FAQ ID -1073

View

Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?

FAQ ID -1056

View

How can I check for purity of RNA isolated using RNeasy Kits?

FAQ ID -1023

View

How can air bubbles in PhosphoProtein Purification Columns be avoided?

FAQ ID -1070

View

How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?

FAQ ID -1062

View

How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?

FAQ ID -1061

View

How can I check the integrity of RNA purified using RNeasy Kits?

FAQ ID -1024

View

How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?

FAQ ID -1087

View

Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?

FAQ ID - 1004

View

Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?

FAQ ID -1012

View

Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?

FAQ ID -1014

View

Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?

FAQ ID -1013

View

Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?

FAQ ID -1009

View

Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?

FAQ ID -1008

View

Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?

FAQ ID -1011

View

Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?

FAQ ID -1010

View

Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?

FAQ ID - 1003

View

Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?

FAQ ID -1006

View

What Disinfectant Solution do you recommend for the BioRobot MDx?

FAQ ID -809

View

What data documentation does QIAGEN's Sequencing Service provide for customers?

FAQ ID -832

View

What are the sequences of the FlexiTube siRNAs?

FAQ ID -851

View

What annealing temperature should be used with the QuantiTect Primer Assays?

FAQ ID -849

View

What is the proper way to turn on or turn off the EZ1 instrument?

FAQ ID -870

View

My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?

FAQ ID -872

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What is the Energy Mix in the EasyXpress Kits?

FAQ ID -846

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What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?

FAQ ID -850

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I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?

FAQ ID -829

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Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?

FAQ ID -855

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Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?

FAQ ID -835

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Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?

FAQ ID -854

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Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?

FAQ ID -841

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Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?

FAQ ID -876

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Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?

FAQ ID -837

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Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?

FAQ ID -875

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Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?

FAQ ID -842

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Will acetone precipitation recommended in the Qproteome Protocols denature Protein?

FAQ ID -807

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Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?

FAQ ID -878

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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?

FAQ ID -862

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Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?

FAQ ID -860

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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?

FAQ ID -864

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Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?

FAQ ID -877

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Will the sequences of the QuantiTect Primer Assays be provided?

FAQ ID -804

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Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?

FAQ ID -836

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What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?

FAQ ID -861

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Will the FastLane Cell cDNA Kit work with suspension cells?

FAQ ID -801

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What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?

FAQ ID -815

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What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?

FAQ ID -873

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What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?

FAQ ID -831

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Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?

FAQ ID -806

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Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?

FAQ ID -865

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Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?

FAQ ID -839

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Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?

FAQ ID -808

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Do I need to use RNase inhibitors with the RNeasy Kits?

FAQ ID -813

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Where can I find QIAGEN products for a specific gene or gene product?

FAQ ID -803

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Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?

FAQ ID -824

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Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?

FAQ ID -834

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Can the QuantiTect Primer Assays be used for detection of genomic DNA?

FAQ ID -811

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Can the FastLane Cell cDNA Kit be used with tissue?

FAQ ID -821

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Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?

FAQ ID -883

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Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?

FAQ ID -879

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895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?

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Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?

-15

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Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?

FAQ ID -881

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Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?

FAQ ID -802

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Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?

FAQ ID -823

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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?

FAQ ID -828

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Can I stabilize adipose tissue using RNAprotect prior to RNA isolation?

FAQ ID -856

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Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?

FAQ ID -838

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Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?

FAQ ID -874

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Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?

FAQ ID -863

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Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?

FAQ ID -812

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Have you tested the effect of inhibitors on PCR performance?

FAQ ID -818

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Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?

FAQ ID -825

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How can I separate PCR fragments that are small and very close in size on an agarose gel?

FAQ ID -800

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Do you have information on in vivo RNA interference experiments?

FAQ ID -817

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I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?

FAQ ID -822

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I left Buffer P1 at room temperature after addition of RNase A, what shall I do?

FAQ ID -859

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How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?

FAQ ID -880

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How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?

FAQ ID -843

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Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?

FAQ ID -893

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Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?

FAQ ID -885

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Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?

FAQ ID -886

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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?

FAQ ID -898

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Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?

FAQ ID -884

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Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?

FAQ ID -896

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Do you have a protocol for the isolation of plasmid DNA from yeast?

FAQ ID -853

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Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?

FAQ ID -887

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Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?

FAQ ID -894

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Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?

FAQ ID -890

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Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?

FAQ ID -888

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Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?

FAQ ID -889

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What are the most commonly used protease inhibitors?

FAQ ID -53

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How should I quantify RNA isolated with RNeasy Kits?

FAQ ID -32

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Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?

FAQ ID -1

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How should I propagate pQE expression plasmids?

FAQ ID -58

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What are the compatibilities of different reagents with Ni-NTA matrices?

FAQ ID -49

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How is "Touchdown PCR" used to increase PCR specificity?

FAQ ID -75

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What should the starting template DNA quality and quantity be for PCR?

FAQ ID -74

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Why do I get smeared PCR products?

FAQ ID -87

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Why does my isolated RNA have a low OD 260/280 ratio?

FAQ ID -97

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When is chloramphenicol amplification of plasmids performed?

FAQ ID -3

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How can I remove imidazole from a protein sample?

FAQ ID -91

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How can I increase the amount of soluble recombinant protein in E. coli expression?

FAQ ID -64

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How can I avoid little or no RNA yields when using an RNeasy Kit?

FAQ ID -28

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What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?

FAQ ID -160

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What are the molecular weights of proteins in the 6xHis Protein Ladder?

FAQ ID -169

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What are your recommendations for cotransfecting several plasmids?

FAQ ID -124

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What is the composition of Buffer EB?

FAQ ID -199

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Is mRNA isolation necessary for sensitive RT-PCR?

FAQ ID -111

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What are the recommended culture and buffer volumes for a very low-copy plasmid?

FAQ ID -168

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Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?

FAQ ID -147

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What is the difference between disruption and homogenization in the RNeasy System?

FAQ ID -139

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Which Anti-His Antibody is the most sensitive for my protein of interest?

FAQ ID -172

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When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?

FAQ ID -100

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Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?

FAQ ID -101

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What is the recommended culture medium for the QIAprep System?

FAQ ID -154

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What is the RNase A concentration and composition of Buffer P1?

FAQ ID -198

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What is the genotype of the EZ Competent Cells?

FAQ ID -157

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What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?

FAQ ID -165

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What is the principle behind Effectene Transfection Reagent?

FAQ ID -184

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What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?

FAQ ID -142

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How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?

FAQ ID -102

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How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?

FAQ ID -134

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How can I improve recoveries when using the QIAquick Kits?

FAQ ID -180

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How can I extract DNA from a polyacrylamide (PAGE) gel?

FAQ ID -120

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How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?

FAQ ID -141

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How can I isolate RNA from 1 gram of plant tissue?

FAQ ID -128

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How can I improve the performance of the HiSpeed QIAprecipitator module?

FAQ ID -144

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How can I improve transfection efficiency using Effectene Transfection Reagent?

FAQ ID -181

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What can I use to isolate RNA smaller than 200 nucleotides?

FAQ ID -115

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What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?

FAQ ID -170

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What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?

FAQ ID -167

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How should RNeasy Kits be stored and how long are they stable?

FAQ ID -103

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Is it possible to cleave the 6xHis-tag from an expressed protein?

FAQ ID -140

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How should I store plant material for DNA isolation using the DNeasy Plant Kit?

FAQ ID -114

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How should QIAGEN Plasmid Purification Kits be stored and for how long?

FAQ ID -192

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What are the features and benefits of the QIAexpress 6xHis Tag System?

FAQ ID -193

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What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?

FAQ ID -148

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Do you have transfection data for QIAGEN Transfection Reagents?

FAQ ID -158

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Do I need to use an RNase inhibitor in my RT reaction?

FAQ ID -119

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Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?

FAQ ID -129

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How can I avoid variable transfection efficiencies with SuperFect Reagent?

FAQ ID -179

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Does QIAGEN offer vectors for direct cloning of PCR products?

FAQ ID -146

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Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?

FAQ ID -133

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Are QIAamp DNA isolation kits suitable for apoptosis studies?

FAQ ID -149

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Can DyeEx be used to clean up labeled DNA other than sequencing templates?

FAQ ID -137

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Are RNeasy spin columns sold separately?

FAQ ID -159

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Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?

FAQ ID -127

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Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?

FAQ ID -130

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Can I use a QIAfilter Cartridge for purifying large plasmids?

FAQ ID -121

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Can I use Omniscript or Sensiscript RT's at a higher temperature?

FAQ ID -116

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How does imidazole affect my quantitation of protein?

FAQ ID -132

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How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?

FAQ ID -176

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How do you ensure that RNeasy buffers are RNase-free?

FAQ ID -113

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How can I purify DNA from soil, food and sewage samples for PCR?

FAQ ID -118

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How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?

FAQ ID -143

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How do I prepare an insert for pQE vectors?

FAQ ID -185

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How should I store Anti·His and Tag·100 Antibodies?

FAQ ID -194

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Do you have a protocol for purification of total DNA from insects?

FAQ ID -1254

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Is your Nuclease-Free Water fluorescence-free?

FAQ ID -1291

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Do you have a protocol for purification of total DNA from crude lysates?

FAQ ID -1255

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How should the GelPilot DNA Loading Dye be handled?

FAQ ID -1287

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What quality level does your Nuclease-Free Water have?

FAQ ID -1292

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What is the pH value of QIAGEN's Nuclease-Free Water?

FAQ ID -1290

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Does GelPilot DNA Loading Dye contain ethidium bromide?

FAQ ID -1286

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Are MaXtract tubes siliconized?

FAQ ID -1298

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Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?

FAQ ID -1299

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Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?

FAQ ID -1211

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What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?

FAQ ID -1205

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What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?

FAQ ID -1220

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What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?

FAQ ID -1221

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Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?

FAQ ID -1232

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Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?

FAQ ID -1213

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How should I prepare buffy coat samples for use on the BioRobot EZ1?

FAQ ID -1249

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Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?

FAQ ID -1225

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Is training of lab personnel included with the purchase of a BioRobot EZ1?

FAQ ID -1239

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Where can I find information about the worktable setup on the BioRobot EZ1?

FAQ ID -1243

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