Product FAQ

 

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How do I determine the amplification efficiency of my qPCR assay?
FAQ ID -2694
How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?
FAQ ID -2696
How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?
FAQ ID -2654
How does HotStart PCR help minimize nonspecific amplification events?
FAQ ID -2676
Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
3248 - What is the difference between sponge, cubic and lamellar phase?
3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
3217 - How long can I store my purified RNA?
3225 - How pure should my protein be for the NexTal CubicPhase kits?
3292-How do I start using the NeXtal CubicPhase kits?
3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
3298-Will the sequence of the QuantiFast Probe Assays be provided?
3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
3243 - What does the Investigator Nonaplex ESS Kit contain?
3244 -What is the average length of a QuantiFast Probe Assay?
3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
Is there also a miRNeasy Plus Universal Kit available?
3261 - Which purification kits are recommended for the therascreen Pyro kits?
3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
3260 - Which purification kits are recommended for the Pyro kits?
3259 - Which probe labels are available for the QuantiFast Probe Assays?
3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
What plug-ins are available for the therascreen Pyro kits?
FAQ ID - 3251
What plug-ins are available for the Pyrosequencing kits?
FAQ ID - 3250
3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
3253 - What species are QuantiTect Probe Assays offered for?
Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit?
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID - 3279
3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID - 3278
3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
3285-How are the genes in each iPSC qBiomarker PCR Array selected?
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT?
FAQ ID - 3282
3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID - 3276
3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
3272 - Are the QuantiFast Probe Assays wet-lab validated?
3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
3215 - What is the starting material for SBTexcellerator HLA Kits?
How can I find out if my gene of interest is expressed in a specific tissue type or cell line?
FAQ ID - 3214
What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
Why should I work with diluted or undiluted screens?
FAQ ID -2419
What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
Where are the somatic mutations published?
FAQ ID -2429
What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?
FAQ ID -2441
What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
What is the PAXgene 96 filter plate?
FAQ ID -2486
What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
How is the hypothesis test performed in the REST software?
FAQ ID -2459
How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?
FAQ ID -2452
How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?
FAQ ID -2421
How do I harvest the crystals from meso phase? Are special tools needed?
FAQ ID -2422
How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
FAQ ID -2495
What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
What dedicated QIAcube Kits are available?
FAQ ID -2337
What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
FAQ ID -2371
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
FAQ ID -2341
How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
Is a special processing protocol needed?
FAQ ID -2523
What is the detection limit for the Investigator Quantiplex Kit?
FAQ ID -2572
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
What are the dimensions of the TissueLyser LT?
FAQ ID -2545
On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?
FAQ ID -2570
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
FAQ ID -2568
What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
FAQ ID -2575
What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
At what temperature should I store the Investigator Quantiplex Kit?
FAQ ID -2578
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
How long is the fixation time?
FAQ ID -2519
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
How long does an Investigator Quantiplex run take?
FAQ ID -2566
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
How can I streamline the quantification workflow for the Investigator QuantiPlex Kit?
FAQ ID -2579
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
How long are the target sequences of Investigator Quantiplex?
FAQ ID -2576
For which real-time cyclers has the Investigator Quantiplex Kit been validated?
FAQ ID -2567
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?
FAQ ID -3095
Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?
FAQ ID -3092
Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples?
FAQ ID -3091
Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?
FAQ ID -3090
Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers?
FAQ ID -3089
What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?
FAQ ID -3096
Can additional positive controls be used for the Investigator IDplex GO! Kit?
FAQ ID -3094
Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?
FAQ ID -3088
Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit?
FAQ ID -3093
What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
Is it possible to fix over a weekend?
FAQ ID -3027
How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
How precise is the QIAgility?
FAQ ID -3063
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
Can I use specific primers with the FastLane Kits?
FAQ ID -3078
What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?
FAQ ID -3087
Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
Is a special processing protocol needed?
FAQ ID -3035
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
When should the N2 bottle used with the QIAxcel Advanced System be changed?
FAQ ID -3001
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
What does PAXgene Tissue FIX contain?
FAQ ID -3020
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
What is the "seed sequence" of a miRNA?
FAQ ID -2262
What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
What is the sample throughput of Pyrosequencing systems?
FAQ ID -2215
What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?
FAQ ID -2216
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
What format are the EasyXtal 15-well tools?
FAQ ID -2219
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
What is a miScript Precursor Assay?
FAQ ID -2214
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
What is a miScript Target Protector?
FAQ ID -2249
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
Does the miScript Target Protector match the target sequence?
FAQ ID -2250
Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
Effects of low A260/A230 ratios in RNA preparations on downstream applications
FAQ ID -2248
How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
How many miScript Precursor Assays do you offer?
FAQ ID -2213
Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
What applications are offered on the QIAcube?
FAQ ID -1403
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
Do I need to buy special kits for the QIAcube?
FAQ ID -1402
Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
What are the dimensions and weight of the QIAcube?
FAQ ID -1407
How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
Can I program my own protocols for the QIAcube?
FAQ ID -1405
How can I load new protocols onto the QIAcube?
FAQ ID -1421
How can I get software updates for the QIAcube?
FAQ ID -1413
How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
How can I decontaminate the QIAcube system?
FAQ ID -1419
Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?
FAQ ID -2031
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
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